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Abstract The aim of this study was to assess the sleep status of middle-aged and elderly adults in Bengbu City, Anhui Province, China, to identify the major dietary patterns, and to analyze the association of different sleep duration and dietary patterns with metabolic syndrome (MetS) and its related influencing factors, as well as to explore the predictive value of sleep duration and dietary patterns. A cross-sectional analysis was performed utilizing data collected from the Community-based Cardiovascular and Health Promotion Study 2019 (COCHPS 2019) conducted in Bengbu. The definition of MetS adhered to the criteria of Guidelines for the Prevention and Treatment of Dyslipidemia in Chinese Adults (2016 Revision). Dietary information was obtained using the Food Frequency Questionnaire (FFQ) to assess dietary intake over the past year. Principal component analysis (PCA) was performed to identify dominant dietary patterns. A logistic regression model was developed to analyze the associations among sleep duration, dietary patterns, and MetS, and a decision tree (DT) model was developed to compare factors affecting MetS and screen people at high risk for MetS. The prevalence of MetS was 13.4% among the 9132 middle-aged and elderly residents over 45 years of age included in COCHPS 2019. Participants were divided into short ( 8 h/d) groups based on their daily sleep duration. Three dietary patterns were identified by PCA, the fruit-milk pattern, the tubers-meat pattern, and the vegetable-cereal pattern. After adjusting for covariables, logistic regression analysis showed that long sleep duration was significantly negatively associated with MetS. The fruit-milk and vegetable-cereal patterns were negatively associated with MetS, whereas the tubers-meat pattern was positively correlated with MetS. The results of the DT model analysis showed that the vegetable-cereal pattern is the most important factor impacting MetS, followed by marital status, the tubers-meat pattern, the fruit-milk pattern, exercise, sleep duration, and gender. The DT model also screened out five types of MetS high-risk groups. The results of our study indicate that normal sleep duration and consumption of either a fruit-milk or vegetable-cereal diet may lower the likelihood of developing MetS in middle-aged and elderly adults.

Abstract Metabolic dysfunction-associated steatohepatitis (MASH) poses challenges for targeted delivery and retention of therapeutic proteins due to excess extracellular matrix (ECM). Here we present a new approach to treat MASH, termed “Fibrosis overexpression and retention (FORT)”. In this strategy, we design (1) retinoid-derivative lipid nanoparticle (LNP) to enable enhanced mRNA overexpression in fibrotic regions, and (2) mRNA modifications which facilitate anchoring of therapeutic proteins in ECM. LNPs containing carboxyl-retinoids, rather than alcohol- or ester-retinoids, effectively deliver mRNA with over 10-fold enhancement of protein expression in fibrotic livers. The carboxyl-retinoid rearrangement on the LNP surface improves protein binding and membrane fusion. Therapeutic proteins are then engineered with an endogenous collagen-binding domain. These fusion proteins exhibit increased retention in fibrotic lesions and reduced systemic toxicity. In vivo, fibrosis-targeting LNPs encoding fusion proteins demonstrate superior therapeutic efficacy in three clinically relevant male-animal MASH models. This approach holds promise in fibrotic diseases unsuited for protein injection.

Objective The existing theoretical calculation method of earth pressure does not take into account the discrete characteristics of pebble stratum such as large porosity and loose structure, and the calculated results are different from the actual earth pressure. Therefore, it is necessary to study the earth pressure calculation method suitable for pebble stratum in Chengdu area. Method Based on Chengdu Rail Transit Line 7, the differences between the earth pressure in deep foundation pit of pebble stratum and the theoretical Rankine value are analyzed, and an earth pressure calculation method for deep foundation pit excavation is established. Using discrete element numerical simulation and statistical data analysis, the distribution characteristics of static earth pressure, passive earth pressure and active earth pressure under the three basic displacement modes of the enclosure structure translation, rotation around the wall top and rotation around wall bottom are obtained. By comparing with the calculation results from Rankine earth pressure theory, the correction coefficient of the earth pressure under three different displacement modes are obtained, and the calculation method of earth pressure correction for deep foundation pit is established. The proposed earth pressure calculation method is verified by further comparison with the on-site measured data. Result & Conclusion The distribution of active earth pressure and passive earth pressure shows strong nonlinearity, which can be described by double fold lines, and the intersection points are located at the buried depth of 8m and 9m respectively. The static earth pressure correction coefficient is distributed between 0.91 and 0.96, not need for correction in use. The active earth pressure correction coefficient is distributed between 0.33 and 0.78, and the passive earth pressure correction coefficient is between 0.52 and 0.91, should be corrected in use. The calculated results of earth pressure in deep foundation pit of pebble stratum are in good agreement with the on-site measured results, providing a reference for similar rail transit projects.

BackgroundWhile observational research has highlighted a possible link between ankylosing spondylitis (AS) and type 2 diabetes (T2DM), the quality of evidence remains limited, and the causal relationship is yet to be established. This study aims to explore the causal link between AS and T2DM, as well as its impact on traits related to glucose metabolism.MethodTo infer a causal relationship between AS and various diabetes-related traits, including type 1 diabetes (T1DM), T2DM, blood glucose levels, fasting glucose, glycated hemoglobin, and fasting insulin, we employed Mendelian randomization (MR) analysis. We sourced GWAS summary data for both exposure and outcome variables from the IEU OpenGWAS database, GWAS Catalog, and FinnGen database. To synthesize the results of the MR analyses, we applied meta-analysis techniques using either a fixed or random effects model. For identifying and excluding instrumental variants (IVs) that exhibit horizontal pleiotropy with the outcomes, we utilized the MR-PRESSO method. Sensitivity analyses were conducted using the MR-Egger method, along with Q and I^2 tests, to ensure the robustness of our findings.ResultsOur analysis revealed a significant association between AS and an increased risk of T1DM with an odds ratio (OR) of 1.5754 (95% CI: 1.2935 to 1.9187) and T2DM with an OR of 1.0519 (95% CI: 1.0059 to 1.1001). Additionally, AS was associated with elevated levels of fasting glucose (beta coefficient = 0.0165, 95% CI: 0.0029 to 0.0301) and blood glucose (beta coefficient = 0.0280, 95% CI: 0.0086 to 0.0474), alongside a decrease in fasting insulin levels (beta coefficient = -0.0190, 95% CI: -0.0330 to -0.0050).ConclusionOur findings collectively underscore the detrimental impact of AS on the development of diabetes, highlighting the critical influence of autoimmune disorders in diabetes onset. This provides profound insights into the pathogenesis of diabetes from an immunological standpoint.

Thoracic ossification of the ligamentum flavum (TOLF) is characterized by ectopic ossification of the ligamentum flavum in the thoracic spine and is considered the main cause of thoracic spinal stenosis and spinal cord disease. Osteoblast specific transcription factor Osterix (Osx) is required for bone formation, and there is no bone formation or ossification without Osx. Surgical intervention is recognized as the only effective method for TOLF treatment with set of complications. However, underlying mechanisms of TOLF are not well understood. This paper summarizes the pathogenesis of TOLF. Some relevant factors have been discussed, such as mechanical stress, genetic susceptibility genes, endocrine and trace element metabolism abnormalities, which may associate with TOLF. More recent studies using proteomics technology and RNA sequencing approach have discovered that some new factors participate in TOLF by upregulation of Osx gene expression including inflammatory factors. TOLF is a unique disease involving multiple factors. On the other hand, studies on TOLF pathogenic mechanism may provide new ideas for finding possible upstream regulatory factors of Osx and further developing novel drugs to stimulate new bone formation to treat osteoporosis.

Maintaining a reliable electricity supply amidst the integration of diverse energy sources necessitates optimizing the stability of power systems. This paper introduces a groundbreaking method to enhance the efficiency and resilience of power grids. The increasing dependence on renewable energy sources poses significant challenges to traditional power networks, thereby demanding innovative solutions to uphold their stability and security. To address these challenges, we propose an architecture that seamlessly unifies dynamic, transient, and static rotor angle stability (RAS) controls into a single, streamlined system. Utilizing reinforcement learning and real-time decision-making, we present Lazy Deep Q Networks (LDQNs) as a novel approach to RAS control. LDQNs provide real-time rotor angle instructions to RAS devices, enabling precise and efficient stability management. The incorporation of mass-distributed energy storage further augments the system's responsiveness and flexibility, mitigating fluctuations and promoting overall stability. This study advances the application of AI methods to RAS control, building on prior research in frequency and voltage stability frameworks. The proposed system outperforms conventional RAS control methods by integrating LDQNs with mass-distributed energy storage, offering superior performance and adaptability. Case studies validate the effectiveness of the unified RAS framework, demonstrating its advantages over traditional approaches across various power system configurations.

Heterotopic ossification of the elbow (HOE) is a complicated pathologic process characterized by extra bone formation in the elbow. Bone formation is a complex developmental process involving the differentiation of mesenchymal stem cells into osteoblasts. The aim of this study was to explore the cellular origin and progression of HOE by single-cell RNA sequencing. We identified 13 clusters of cells in HOE and further analyzed the subclusters for 4 of the main cell types. Six subclusters of osteoblasts, nine subclusters of chondrocytes, six subclusters of fibroblasts, and five subclusters of mononuclear phagocytes (MPs) were identified and analyzed. The new findings on osterix (OSX) and SOX9 expression in osteoblast subclusters and chondrocyte subclusters indicate that HOE is mediated through endochondral ossification. Further identification of the corresponding signature gene sets of distinct subclusters indicated that subclusters of osteoblasts_3, osteoblasts_4, osteoblasts_5, and osteoblasts_6 are relatively more mature during the osteoblastic progression of HOE. The trajectory analysis of the osteoblasts demonstrated that some genes were gradually downregulated, such as CRYAB, CCL3, SFRP4, WIF1, and IGFBP3, while other critical genes were upregulated, such as VCAN, IGFBP4, FSTL1, POSTN, MDK, THBS2, and ALPL, suggesting that these factors may participate in HOE progression. Cell–cell communication networks revealed extensive molecular interactions among the 13 HOE clusters. Ligand–receptor pairs for IL6, COL24A1, COL22A1, VWF, FZD6, FGF2, and NOTCH1 were identified, suggesting that multiple signaling pathways may be involved in HOE. In conclusion, this study provided the cellular atlas for HOE. We have established a greater extent of the heterogeneity of HOE cells than previously known through transcriptomic analysis at the single-cell level. We have observed gradual patterns of signature gene expression during the differentiation and maturation progression of osteoblasts from stem cells in HOE with higher resolution. The cell heterogeneity of HOE deserves further investigation to pave the way for identification of potential targets for HOE early diagnosis and therapeutic treatment.

The neurological complications following cardiovascular surgery can significantly affect the prognosis of patients. As a convenient and non⁃invasive intraoperative neurological monitoring method, transcranial Doppler ultrasonography (TCD) can provide real⁃time assessment of cerebral hemodynamics and microemboli signals, guide the development of neuroprotective strategies, and reduce the brain injury events. This review systematically summarizes the basic methods and technical performance of TCD monitoring, its clinical application in cardiovascular surgery, as well as the main limitations and future trends of this technology, in order to improve perioperative safety.

Abstract Background Transseptal puncture (TSP) is a crucial technique for catheter ablation of atrial fibrillation (AF). Although intracardiac echo (ICE) facilitates a safe and accurate TSP, it is not widely used in developing countries because of the expense. This study evaluated the efficiency and safety of a novel cardiac multidetector computed tomography (MDCT)‐guided TSP during AF catheter ablation. Methods The study consisted of two cohorts. In the index cohort, TSP procedure was performed under the guidance of ICE, and we recorded the angulation of right anterior oblique of X‐ray projection. In the validation cohort, we compared the efficiency and safety of TSP guided by MDCT‐calculated angulation with propensity‐score‐matched patients who underwent TSP guided by ICE. Results We included 50 patients in the index cohort, and the mean angles of interatrial septum (IAS) measured from MDCT and ICE were 34.8 ± 6.3 and 35.1 ± 6.5, respectively. In the validation cohort, 376 patients were enrolled in the MDCT‐guided group and ICE‐guided group. Both groups had 1 case of cardiac tamponade. The mean axial plane angle was 35.46 ± 6.17 degrees, which was not influenced by age, gender, BMI, and LA size, while a moderate positive linear correlation between EF and the axial plane angle (R2 = 0.14, p = .006). Conclusion Cardiac MDCT can provide a clear vision of IAS orientation, and provide the appropriate RAO angle and height for TSP. The efficiency and safety of our MDCT‐guided TSP were comparable to ICE‐guided TSP, which may serve as an alternative method for TSP with ICE unavailable.

Nucleoside free radicals have important functions in organisms and play a crucial role in DNA damage. Accurately understanding the structure of nucleoside free radicals is crucial for studying their properties, reaction mechanisms, and interactions with other biomolecules. The high vacuum in the mass spectrometer provides an inert environment for free radicals. Further tandem mass spectrometry combined with theoretical calculation is crucial for achieving their structural informations. This article reviewed gas phase studies of base and nucleoside radicals based on mass spectrometry in the past two decades, and provided a detailed introduction and comparison of their production methods, structural research methods and progress. Different methods were developed for the generation of the free radical ions, including collision induced dissociation of precursor ions of ternary transition metal complexes or those introducing free radical initiators, electron transfer dissociation of precursor ions charge-tagged, and UV photodissociation of precursor ions including C-I bonds. Besides, the methods for studying the structure of free radical ions in gas phase have gradually enriched, including commonly used tandem mass spectrometry, UV-visible and IR photodissociation spectroscopy. Based on the above methods, many different base and nucleoside free radical ions were generated and structurally determined in gas phase. On the other hand, the research of the highly active free radical intermediates and the selective generation of free radical isomers still pose challenges, and there is an urgent need to develop new experimental method.

Abstract Objective By reviewing the literature analyzing vancomycin powder for preventive surgery, the effect of this method on reducing the infection rate after TJA was systematically evaluated to provide a basis for future clinical work. Methods Using PubMed, Medline, Elsevier, and CNKI, with the following mesh words: “vancomycin”, “local / intraoperative / topical / intrawound”, “TJA”, “TKA”, “THA”, “total joint arthroplasty”, “total knee arthroplasty”, “total hip arthroplasty”, “infection”, and “SSI”, to search for case-control research papers on the impact of prophylactic application of vancomycin powder on the incidence of postoperative infection, we compared the overall infection rate in the literature by using RevMan 5.3 meta-analysis software and analyzed the impact of vancomycin on the infection rate of different parts and types of TJA according to different subgroups. Results A total of 22 qualified studies were selected; twenty-five studies compared the effect of prophylactic use of vancomycin powder on infection rates after TJA. There were 23,363 cases in total, including 9545 cases in the vancomycin group and 13,818 cases in the control group. The results of the meta-analysis showed that the possibility of postoperative infection after prophylactic use of vancomycin powder was significantly lower than that without vancomycin risk ratio: 0.38 [0.23,0.59], P

In response to the complex geographical environment and great difficulty in risk control of high consequence areas(HCAs) of mountainous pipelines, accurate identification of the HCA type and grade, if achievable, could effectively improve the operation safety of pipelines. However, the existing identification standards have certain limitations when applied to the mountainous oil and gas pipelines.Therefore, it was necessary that Myanmar-China Oil and Gas Pipeline(China section) was employed for the case study. According to the Oil and Gas Pipeline Integrity Management Specification(GB 32167—2015), the HCAs of oil and gas pipelines were identified and analyzed to identify the HCA types and the characteristics of identification items to be concerned. The analysis and classification were made for the environmental characteristics in mountainous areas, so as to find out the influence of the mountainous environment on oil and gas pipeline leakage. Additionally, the limitations of the current HCA identification standards for mountainous oil and gas pipelines were outlined here.The study results have certain reference significance for the identification and management of HCAs of mountainous pipelines, as well as the potential standard revisions.

Traditional audio processing technology often faces the challenge of efficiency and accuracy in the face of large-scale audio data and complex sound content. The purpose of this study is to develop the key technology of audio retrieval and vocal music teaching system based on sensor network, aiming at the rich audio data and real-time monitoring function provided by sensor network. The sensors suitable for audio retrieval and vocal music teaching were selected and rationally arranged in the designated position to obtain accurate and comprehensive audio data. The algorithm is also optimized and improved to improve the performance and stability of the system. The accuracy and efficiency of audio retrieval can be effectively improved by using sensor networks. The vocal music teaching system based on sensor networks can provide comprehensive and accurate sound evaluation and guidance. Good results have been obtained in the practical application of this method, which proves its feasibility and effectiveness in the field of audio retrieval and vocal music teaching.

ObjectiveThis study analyzed the current research hotspots and future development trends of the therapeutic effects of microRNA on PNI axonal regeneration through bibliometric methods. Moreover, the current advantages and disadvantages of this field as well as future development prospects are discussed in depth.MethodsCiteSpace V and VOSviewer were used as bibliometric tools to complete the analysis of the research focus and direction of the published articles. To supplement, sort out, and summarize, we analyzed the research status of the study on the application of microRNAs for axonal regeneration after peripheral nerve injury from 2013 to 2023.ResultsA total of 207 publications were retrieved from the Web of Science database. After exclusion and screening, a final selection of 174 articles that met the research criteria. These 174 articles were authored by a total of 846 individuals, representing 24 countries and 199 institutions. Additionally, this study presents information on the annual publication output, country distribution, top 5 contributing authors, top 5 most cited articles, and top 10 contributing institutions.ConclusionAs one of the hottest topics today, microRNAs have become the current research hotspot in neural inflammation, neural cell repair and regeneration, neural protection, and functional recovery. With more investment in research in this field, more high-quality articles will be published in both domestic and international outstanding journals, which will bring a new era for the treatment of peripheral nerve injury.

Brain and heart comorbidity is one of the major public health problems in China, which seriously threatens national health. After many years of exploration, the concept of "synchronous treatment for brain and heart" has become more and more accepted. This article summarizes the pathophysiological mechanism of brain ⁃ heart interaction, the clinical and research practice of the theory of "synchronous treatment for brain and heart", and its future development direction, and jointly help the diagnosis and treatment of brain and heart comorbidity.

Abstract Objective The persistence of HPV infection is a significant etiological factor in the development of cervical cancer. The present study investigated the prevalence and genotype distribution of human papillomavirus (HPV) in a cohort of 164,137 unvaccinated women from Wenzhou, aiming to provide guidance for clinics in the cervical cancer screening and HPV vaccination strategies. Methods The present retrospective study included a total of 164,137 women, comprising 118,484 outpatients and 45,653 healthy female subjects recruited from 2015 to 2020. Cervical exfoliated cells were collected from these participants for subsequent DNA extraction. The extracted DNA samples were underwent analysis using a fluorescence in situ hybridization method, encompassing the detection of 27 HPV genotypes. Results The overall prevalence of HPV was 17.35%; this corresponded to a prevalence of 19.10% in the outpatient group and 12.82% in the healthy female group. Among the outpatient group, the five most prevalent HPV genotypes were identified as HPV 52, 58, 16, 53, and 61. In the healthy female group, the five most common HPV genotypes were found to be HPV 52, 53, 58, 61, and 81. Additionally, it was estimated that the highest rate of HPV infection occurred among individuals aged between 10 and 19 years old (44.65%) and those aged between 60 and 69 years old (27.35%). Conclusions The prevalence of HPV in this region is substantial; therefore, it is imperative to implement scientifically sound and rational clinical interventions such as vaccination. Routine cervical screening should be performed to prevent the development of cervical intraepithelial neoplasia resulting from persistent infection with high-risk HPV, particularly in women with gynecological diseases and those over 60 years old.

Objective: This study aimed to improve the oxidation, exploitation, and utilization of pumpkin seed oil (PSO). Methods: Pea protein isolate (PPI) -Tannic acid (TA) stabilized Pickering emulsion with Maltodextrin (MD) was used as the filling material, and prepared pumpkin seed oil microcapsules by spray drying. Results: After spray drying the Pickering emulsion, the smooth surface of the medium shell structure of microcapsule powder can be obtained. By increasing TA concentration, microcapsules showed smaller particle size [(32.00±0.28) μm], lower moisture content [(1.970±0.043)%], and higher bulk density [（0.725±0.014) g/cm3]. The FFA release rate of pumpkin seed oil microcapsules with different TA content was in the range of 39.63%~69.91%, and it slowed down when TA concentration increased. Compared with the PSO encapsulated in PPI, the PPI-TA composite used as the shell material improved the thermal stability, oxidation resistance and oxidation stability of PSO. Conclusion: These results indicated that PPI-TA-MD microencapsulation technology can improve the antioxidant capacity and oxidation stability of PSO.

Post-acidification is one of the most important factors affecting the sensory quality, flavor characteristics and stability of yogurt during shelf-life. Non-thermal treatments are novel methods to control post-acidification, which can maintain the original nutritional properties and sensory quality of food to the maximum extent. In this paper, the mechanism of post-acidification of lactic acid bacteria was reviewed. The mechanism and recent research progress of non-heat treatment processes such as ultra-high pressure, ultrasound, pulsed electric field, CO2 treatment, irradiation were analyzed and provided theoretical guidance for the development and industrial application of non-heat acid control technology.

Graphitic carbon nitride(g-C3N4) loaded by metal atom has been applied as an effective single atom catalyst. In the work, the complexation behaviors of g-C3N4(CN) with metal ions have been examined by relativistic density functional theory. The formed ［M(CN)］z+(M=Sc, Y, La, Ac and U; z=2 and 3) have been explored for their structures, metal-ligand interactions and redox properties. It is found that the metal ion has been rendered by six M-N dative bonds, each of which has the bonding strength ranging from -0.79 to -0.47 eV. Regarding Sc complexes, one-electron reduction mainly occurs around the metal center, while La and U complexes have metal-modified ligand reduction mechanism; both metal and ligand in Y complex are approximately equally reduced; in contrast, the ligand of Ac complex gets the reducing electron, and the metal is redox-innocent. This agrees with the fact that there are no experimental reports on Ac(Ⅱ) complexes. Electronic-structure analyses indicate that ［U(CN)］3+ bears three high-lying single U(5f) occupied orbitals, suggesting the trivalent uranium center. However, despite having four single U(5f) occupied orbitals, ［U(CN)］2+ still possesses considerable ligand contribution, leading to the uranium oxidation state mediating between Ⅱ and Ⅲ.

Abstract Accurate and efficient detection of canopy gaps is essential for understanding species regeneration and community dynamics in forests. Unoccupied aerial vehicles (UAVs) equipped with visible light (e.g., RGB) cameras have the potential to be one of the most cost‐effective approaches for detecting gaps. However, current gap‐detection methods based on spectral, textural, and/or structural information derived from UAV RGB imagery are unreliable in species‐rich forests with complex terrain due to high spectral complexity and topographic shadowing. Here, we compared the performance of four methods, including pixel‐based supervised classification (PBSC), object‐based classification (OBIA), Canopy Height Model thresholding classification, and HSTAC [a novel method we developed which combines Photographic Height (H), Spectral (S), and Textural (T) information for Automatic Classification (AC)] for characterizing canopy gaps in a 20‐ha permanent subtropical forest plot of eastern China. All classification results were evaluated through a comparison with canopy gaps detected from both field surveys and UAV‐borne LiDAR data. Among the four classification methods, HSTAC performed best in terms of detection efficiency (96% overall accuracy when compared to field data and 85% when compared to the LiDAR data), classification accuracy (3–18% improvement compared to alternative methods), and speed (1–1.5 h faster on the same machine). Of the four topographic factors (elevation, slope, aspect, and convexity), elevation was the one that most affected the accuracy of canopy gap detection. The errors of PBSC classification mainly came from the gaps at low elevations, while OBIA located the position of gaps well but overestimated their sizes. Overall, HSTAC avoids many of the inherent limitations of current state‐of‐the‐art methods and can accurately map canopy gaps in diverse subtropical forests with complex terrain. Our study provides a suitable way for long‐term forest canopy monitoring, real‐time applications, and contributes to a better understanding of forest plant community assembly and succession dynamics.

Functional dystonia is one of the most common phenotypes of functional movement disorder (FMD). Multiple clinical manifestations of functional dystonia are complex and some symptoms overlap with idiopathic dystonia. The diagnosis of functional dystonia is challenging and the overall progenosis is poor. This article reviews the clinical manifestations, auxiliary examination, diagnosis and differential diagnosis, treatment and prognosis of functional dystonia, in order to improve clinical cognition and promote the multi‑disciplinary team (MDT) of functional dystonia.

Abstract This study was aimed at assessing the risk factors for hypertension (HTN) and prehypertension (PHT) in order to clarify the role of lipid accumulation product (LAP) in the development of HTN among nondiabetic individuals. A large cross‐sectional study was conducted in community health service centers in urban Bengbu, Anhui Province, China. All participants completed an interview questionnaire and procedures to obtain physical measurements and biochemical indicators. Multivariate logistic regression was used to test for the prevalence of HTN and PHT in relation to each quartile increase in LAP level and family history of HTN. The resulting interaction effects were evaluated by relative excess risk of interaction (RERI), attributable proportion due to interaction (AP), and synergy index (SI). A total of 7733 subjects were enrolled in the study. The overall prevalence rates of PHT and HTN were 37.1% and 24.8%, respectively. After adjusting for confounding factors, multinomial logistic regression analysis showed that compared with subjects in LAP quartile 1, those in quartile 3 (OR, 1.257; 95% CI, 1.062–1.494) and quartile 4 (OR, 1.323; 95% CI, 1.101–1.592) had a significantly higher risk for HTN (p for trend

Generalized Lyapunov matrix equations appear in the fields of controllability analysis and model reduction of bilinear systems, stability analysis and optimal stabilization of linear stochastic systems, etc. The author studies the numerical solution of the generalized Lyapunov matrix equation combined with the evaluation of the Russian spoken language self-study system. Taking the empirical analysis as the research object, we selected 120 oral test samples from 12 colleges and universities in the 2020 national four-level unified test of Russian majors, compared with the teaching syllabus and the examination syllabus, the data were analyzed from the perspectives of speech speed, vocabulary, grammar and vocabulary errors, and a series of important conclusions were drawn, according to the specific problems exposed by the students in the oral test, the current situation of oral Russian teaching is discussed from the aspects of oral teaching materials, oral teachers and oral teaching methods.

Abstract Background The triglyceride glucose (TyG) index has been regarded as an effective proxy of Insulin resistance (IR). Studies on the TyG index, obesity and the risk of prehypertension (PHT) in elderly people are not apparent currently. The study sought to investigate the predictive value of TyG index and the associations with PHT risk and obesity. Methods A community-based cross-sectional study was conducted in Bengbu City, Anhui province, China. Participants older than 65 years accepted questionnaire surveys, physical examinations and blood biochemistry tests. Based on the testing results, indicators including BMI (body mass index), WC (waist circumference), WHtR (waist-to-height-ratio), LAP(Lipid accumulation products) and TyG were calculated. Residents were classified into quartiles by their TyG indexes. Receiver operating characteristic curve (ROC) analysis was carried out to predict obesity indices for PHT. The three additive interaction indicators, RERI (relative excess risk due to interaction), AP (attributable proportion due to interaction) and S (synergy index) were used to assess the interaction impacts. Results Two thousand six hundred sixty-six eligible elderly people were included in study and the prevalence of PHT was 71.04% (n = 1894). With increasing TyG index quartile, PHT became more prevalent. After adjusting for confounding factors, the prevalence of PHT risk with TyG levels in the fourth quartile (Q4, male: 2.83, 95%CI: 1.77–4.54; female: 2.75, 95%CI:1.91–3.97) was greater than that in the first quartile (Q1:ref). TyG index (AUC: 0.626, 95%CI: 0.602 to 0.650) was superior than BMI (AUC: 0.609, 95%CI: 0.584 to 0.633) in predicting PHT among females. Eventually, there were significant interactions of TyG index with obesity in males (General obesity: AP = 0.87, 95%CI: 0.72 to 1.02, S = 10.48, 95%CI: 3.43 to 31.97; Abdominal obesity: AP = 0.60,95%CI: 0.38 to 0.83, S = 3.53, 95%CI: 1.99 to 6.26) and females (General obesity: AP = 0.89, 95%CI: 0.79 to 0.98, S = 12.46, 95%CI: 5.61 to 27.69; Abdominal obesity: AP = 0.66, 95%CI: 0.51 to 0.82, S = 3.89, 95%CI:2.54 to 5.98). Conclusion TyG index and PHT risk are tightly correlated. The risk of chronic disease in the elderly can be decreased by early detection of PHT utilizing the TyG index. In this research, the TyG index was more predictable than other indicators of obesity.

Ovarian cancer (OC) represents a significant health challenge, characterized by a particularly unfavorable prognosis for affected women. Accumulating evidence supports the notion that inflammation-related factors impacting the normal ovarian epithelium may contribute to the development of OC. However, the precise role of inflammatory response-related genes (IRRGs) in OC remains largely unknown. To address this gap, we performed an integration of mRNA expression profiles from 7 cohorts and conducted univariate Cox regression analysis to screen 26 IRRGs. By utilizing these IRRGs, we categorized patients into subtypes exhibiting diverse inflammatory responses, with subtype B displaying the most prominent immune infiltration. Notably, the elevated abundance of Treg cells within subtype B contributed to immune suppression, resulting in an unfavorable prognosis for these patients. Furthermore, we validated the distribution ratios of stromal cells, inflammatory cells, and tumor cells using whole-slide digitized histological slides. We also elucidated differences in the activation of biological pathways among subtypes. In addition, machine learning algorithms were employed to predict the likelihood of survival in OC patients based on the expression of prognostic IRRGs. Through rigorous testing of over 100 combinations, we identified CXCL10 as a crucial IRRG. Single-cell analysis and vitro experiments further confirmed the potential secretion of CXCL10 by macrophages and its involvement in lymphangiogenesis within the tumor microenvironment. Overall, the study provides new insights into the role of IRRGs in OC and may have important implications for the development of novel therapeutic approaches.

Objective: In order to improve the accuracy and operating efficiency of the egg crack detection method based on computer vision. Methods: Used poultry egg simulation impact equipment to obtain cracked eggs, and collected images of cracked eggs and intact eggs from different angles through egg dynamic image acquisition equipment. Then, the YOLO-v5, ResNet and SuffleNet models for cracked egg image recognition were established for the original and the preprocessed egg images, respectively. After that, the recognition accuracy and the adaptability for original egg images recognition of different models were compared. Results: The YOLO-v5, ResNet and SuffleNet models could effectively identify the preprocessed cracked egg images, and the accuracy rates of verification set were 98.8%, 97.8% and 99.4% respectively. For original eggs images, the ResNet model had a low recognition accuracy, while the SuffleNet model had the highest recognition accuracy, which was up to 99%. Conclusion: Among the convolutional neural network models, SuffleNet model is most suitable for cracked egg image recognition, and the egg image preprocess is not necessary. This study provides a reference for the further improvement of crack egg detection methods based on computer vision.

Objective: A solid phase extraction-gas chromatography/mass spectrometry （GC-MS） method was developed for the simultaneous determination of 24 pesticide residues in coffee peel. Methods: The sample was wetted with water, extracted with acetonitrile, the extract solution is concentrated and then purified with ProElut TPC solid phase extraction column, eluted with acetonitrile and toluene=3∶1, after concentration, the sample was determinated by GC-MS and the peak area was measured by internal standard method. Results: The 24 pesticides were separated well within 28 minutes. The residue of pesticide was in the range of 0.016～0.400 mg/kg, the linearity of the method was good, the correlation coefficient r was between 0.997 9～0.999 9, the quantitative limit was between 0.01～0.03 mg/kg, when the concentration of pesticide is 0.03 mg/kg and 0.30 mg/kg, the recovery rate is between 65.2%～108.5% and 81.4%～105.7%, The relative standard deviation of intra day precision is between 3.7%~6.8% and 2.5%~5.9%, and the relative standard deviation of daytime precision is between 4.2%~7.2% and 2.8%~6.4%, compared with acetonitrile∶toluene=3∶1, as eluting solvent and ProElut TPC as purification column, the elution and purification efficiency were the highest, and the recovery was 95%. Conclusion: The performance parameters of the method meet the requirements of national standards, and the determination results are accurate and reliable. It can be applied to the qualitative and quantitative analysis of multi-component pesticide residues in dried coffee peel.

Objective: This study aimed to explore the differences in the aroma of watermelon seeds under different thermal processes. Methods: The analysis technics such as Headspace-Solid Phase Micro Extraction-Gas Chromatography-Olfactory-Mass Spectrometry (HS-SPME-GC-O-MS) and Electronic Nose (E-nose) were applied. Results: Thermal processing changed the flavor of watermelon seeds from light fragrance to strong roasted flavor; A total of 18 categories including 115 volatile compounds were detected with the help of HS-SPME-GC-O-MS, among which pyrazines, alcohols and aldehydes accounted for 34.25%, 21.07% and 10.99% of the total volatile compounds, respectively; In addition, 63 volatile compounds were discovered in watermelon seeds after microwave treatment, and pyrazines accounted for 53.31% among all these volatile compounds. Conclusion: Thermal processing treatment showed significant influence on the aroma of watermelon seeds, and the aroma of watermelon seeds after microwave treatment was more intense than air-frying and baking treatment.

Compared with the advanced and sophisticated industrial production, agriculture and food production appear backward and sloppy, and agricultural production management methods are lacking. The fourth industrial revolution, represented by information technologies such as intelligent perception, Internet of Things, block chain, big data and artificial intelligence, has empowered traditional industries, and agriculture has stepped into a new digital, intelligence and even wisdom, which could provide a new opportunity to improve agricultural production management. Existing agricultural production management research is mostly limited to the study of concept definition, applicable theory and other issues. The development of information technology has built up a low-level Internet of Things with certain coverage, which brings some convenience, but cannot effectively support agricultural management and decision-making due to data silos, data waste, data abuse, data falsification and other thorny issues. From the perspective of top-level design and system management, this paper addressed the problems in the development of smart agriculture, conceptualized the characteristics of the event and natural event chains in the agricultural industry chain. This paper also proposed for the first time an event-correlation management approach and its wisdom strategy. The implementation of the event-correlation method included five aspects, including goal alignment, index systematization, asset linkage, process linkage and effect traceability. The application of the event-correlation management approach and its wisdom in the 5T management standard designation for Jilin rice brand building and the “six-step fresh rice precision control technology” innovation system of Wilmar rice industry have shown that it can improve the culture of rice cultivation, and systematically reduce losses to improve quality and increase economic benefits.

The influence of science and technology on cognitive change has a long history, which lead to forming philosophy of science and philosophy of technology respectively. The development of grain storage technology in China has gone through three stages: the initial exploration before the reform and opening up, the construction of intellingence in the past 10 years modern granaries in 30 years after the reform and opening up, and the creation of digital. The scientific and technological progress of grain storage in China has been witnessed by all, including building a modern granary with "four in one" new grain storage technology, to forming the world's largest grain and food networking with information technology, original multi-field coupling and bio-field theory, and realizing the dynamic supervision of grain reserves with grain cloud image scanning technology. In the new era, the political and economic situations have not changed significantly in the past century, and the new requirements of China's second centenary goal for food security and the impact of the fourth industrial revolution have made the trend of food science and technology becoming more and more intelligent. Based on the accumulation of scientific and technological achievements in grain storage practice for more than 40 years in China, combined with the team's research on multi-field coupling and bio-field theory of grain storage, dynamic supervision of cloud image of grain reserves, 5T information management of natural block chain for grain storage, and intelligent EAT window measurement and control methods for grain drying, this paper attempted to take the basic idea of "one divides into two, two divides into three, and three generates changes" on the basis of deconstructing the micro and macro ternary microstructure of grain storage system. Taking the joint driving of mechanism and data as the intelligent strategy, the granary philosophy is introduced to improve the recognition of existing scientific and technological achievements. The granary philosophy also guided the exploration of grain storage technology in the new era, and provided support for building a high-quality intelligent food system. The granary philosophy is a deep reflection on the practical situation of grain storage, and has certain universality, which originates from grain storage practice, but is not limited to grain storage practice.

Probiotic fermented milk is an important carrier of probiotics. It enhances the nutritional and health properties of fermented milk by improving the balance of host intestinal microflora and promoting the metabolism of beneficial ingredients. This review expounds the auxiliary effects of probiotics fermented milk in improving intestinal function, regulating body immunity, assisting in treating metabolic diseases and improving mental system diseases, etc., in order to provide theoretical support for expanding the application of probiotics fermented milk.

A recent study of ours reported that pH of common salts varies with concentration at room temperature. It can either increase in case of basic salts or decrease in case of acidic salts. In this study, pH values of four kind of potassium salts were investigated and their pH values increased with salt concentrations. It was reasoned that the concentration of hydrogen ions equalled the concentration of positive charges/protons in a solution, so a relationship between the activity of hydrogen ions and charge activity was developed. Further, the well-established equations for pH with hydrogen-ion activity and electrostatic/cell potential were extended to charge activity. Potential energy, the product of charge activity and electrostatic/cell potential, was presented and discussed.

Objective: This study aimed to explore the effect of processing methods on the quality of pumpkin seed kernels. Methods： In this study, pumpkin seed kernels were chosen as the research object and the effects of air-frying （160 ℃, 0~7 min） and microwave treatment （700 W, 0~7 min） on the physical and chemical properties of proteins were investigated. Results： The experimental results showed that: after air-frying and microwave treatment, polyphenol, flavone, polysaccharide contents, as well as sensory scores all increased at the beginning, then decreased gradually, and reached the peak values when being treated by air-frying for 4 min and microwave treatment for 5 min, respectively. Under the optimal conditions, the degree of lipid oxidation was lower after microwave treatment, the protein aggregation phenomenon was more obvious, and the structure tended to be disordered and stretched, with a higher degree of lipid oxidation and better digestibility, while air-fried nuts had a stronger roasted flavor. Conclusion： The results indicated that proper air-frying and microwave treatment could improve the flavor and digestive properties of pumpkin seed kernels, and then improve the quality of pumpkin seed kernels.

The nozzle is one of the key components in the integrated design of the aircraft body/propulsion,which directly affects the thrust-drag matching and pitching moment matching and other characteristics of the aircraft.In order to improve the efficiency of the optimal design of the nozzle,a multi-objective optimization model based on multi-fidelity surrogate model is established.The CFD results based on coarse mesh and inviscid model are used as low-fidelity data,and the CFD results based on fine mesh and SST k-ωturbulence model are used as high-fidelity data,the Cokriging models are constructed with the maximization of thrust coefficient,lift coefficient and pitching moment coefficient as optimization objectives,and the Pareto solution set is obtained by combining NSGA-Ⅱalgorithm.The optimization results show that the three aforementioned objectives are improved by 2.94%,13.0%and 40.6%respectively with the error less than 0.5%,and the sensitivity analysis is completed;the optimized flow field wave system structure is more complex which changes the wall pressure distribution law;compared with Kriging model,Cokriging model has considerable prediction performance and saves 62%of the construction cost.

Objective To investigate the clinical efficacy of intra-articular irrigation combining with passive mouth-opening training in the treatment of acute temporomandibular anterior disc displacement without reduction concomitant with limited mouth opening in elderly patients. Methods From January 2019 to January 2022, 16 elderly patients diagnosed as acute temporomandibular anterior disc displacement concomitant with limited mouth opening and underwent intra-articular irrigation and passive muscle stretching were retrospectively reviewed. General information, past history, history of present disease, clinical and radiologic examinations were collected. Maximum mouth opening and pain degree (VAS score) were recorded before, immediately after and 1 week after treatment. Results The mean maximum mouth opening of 16 patients was (25. 13±1. 93) mm before treatment, (37. 31±1. 82) mm immediately after treatment and (43. 56±2. 61) mm at 1 week, respectively, with significant difference (P

By using image analysis technology and automatic control technology, the artificial intelligence technology was applied to the detection of unsound wheat kernels. The automatic, rapid and nondestructive detection instrument for unsound wheat kernels was developed. Through the verification of accuracy, repeatability, stability, difference between two instruments and other related performance parameters, the results showed that the instrument had stable performance. The accuracy, repeatability, stability and difference between two instruments met the requirements of industry standards. The operation was simple, and the detection speed was fast. It can overcome the problems of strong subjectivity, poor repeatability and poor inspection consistency among different personnel with manual checking, achieving the automatic and rapid nondestructive testing of unsound wheat kernels, and meeting the testing demand of grain collection and storage enterprises, processing enterprises and inspection agencies.

Objective To observe the effect of Tonifying deficiency and Zhengqi porridge on immune function reconstruction of HIV/AIDS patients with Qi deficiency. Methods Sixty (Human Immunodeficiency Virus/ Acquired Immunodeficiency Syndrome)HIV/AIDS patients admitted to the center for Disease Control and prevention in Huichuan District of Zunyi City were randomly divided into control group and treatment group by random number table. All patients received the highly active anti-retroviral therapy, and those in the treatment group were given Tonifying deficiency and Zhengqi porridge. The CD4+ T-cell count was measured before, 3 months and 6 months after intervention. The Effective reconstitution of immune function was analyzed. Results After 3 and 6 months of treatment, the immune reconstitution in the treatment group was better than that in the control group for patients with CD4+ T-cell count of 100-500/mm3 (P＜0. 05). Conclusion Tonifying deficiency and Zhengqi porridge can promote the reconstruction of immune function in HIV / AIDS patients with Qi deficiency and help to restore immune function. (目的 探讨补虚正气粥对人类免疫缺陷病毒(HIV)感染及艾滋病(AIDS)气虚型患者的免疫功能恢复作用。方法 选择遵义市汇川区疾病预防控制中心60名HIV/AIDS患者, 随机将患者分为治疗组和对照组, 各30例。对照组按传统方法进行HAART(高效抗反转录病毒)治疗, 治疗组则在对照组治疗的基础上加用补虚正气粥。于干预前、干预后3个月和干预后6个月比较两组患者CD4+T淋巴细胞计数值和免疫功能重建有效率。结果 对于干预前CD4+T淋巴细胞计数100～500个/mm3的患者, 在介入3个月和6个月, 治疗组免疫功能的恢复较对照组好(P＜0. 05)。结论 补虚正气粥对HIV/AIDS气虚型患者免疫功能重建有促进作用, 有助于免疫功能的重建。)

Abstract Background Lipid accumulation product (LAP) is considered to be a new convenient useful indicator to assess the visceral fat. Therefore, we aimed to evaluate the risk factors of impaired fasting glucose (IFG) and diabetes, and explore the possible interacting influences of LAP with other factors on the risk of IFG and diabetes among Chinese normotension adults. Methods A multistage stratified cluster sampling method was conducted to select urban residents in Bengbu, China. For each eligible participant, data on questionnaire survey, anthropometric measurements and laboratory tests were obtained. The effects of body mass index (BMI), waist circumference (WC), waist to height ratio (WHtR) and LAP for predicting IFG and diabetes were performed by multiple logistic regressions and receiver operating characteristic (ROC) analyses. The interaction effects were evaluated by relative excess risk of interaction (RERI), attributable proportion due to interaction (AP) and synergy index (SI). Results Six thousand, four hundred sixty-seven normotension subjects (2695 men and 3772 women) were enrolled in our study, the prevalence of IFG and diabetes were 9.37% and 14.33%, respectively. When assessed using ROC curve analysis, LAP exhibited higher diagnostic accuracy for identifying IFG and diabetes than BMI, the area under the AUC curve was 0.650 (95% CI: 0.637 to 0.662). After adjustment for age, sex, educational level and other confounding factors, multivariate logistic regression analyses indicated that subjects with the fourth quartile of LAP were more likely to develop IFG (adjusted OR: 2.735, 95% CI: 1.794–4.170) and diabetes (adjusted OR: 1.815, 95% CI: 1.297–2.541) than those with the first quartile. A significant interaction between LAP and family history of diabetes was observed in participants (RERI = 1.538, 95%CI: 0.167 to 3.612; AP = 0.375, 95%CI: 0.118 to 0.631; SI = 1.980, 95%CI: 1.206 to 3.251). However, a significant interaction between LAP and abdominal obesity was indicated by the value of RERI (1.492, 95%CI: 0.087 to 3.723) and AP (0.413, 95%CI: 0.014 to 0.756), but not the value of SI (1.824, 95%CI: 0.873 to 3.526). Conclusion Our results demonstrated that there might be synergistic effect between LAP and family history of diabetes on the risk of IFG and diabetes.

The measurement of gas phase collision cross section (CCS) of ions can provide complementary structure information to those typically obtained from mass spectrometry or tandem mass spectrometry. Thus, the ion mobility spectrometry is typically coupled with a mass spectrometer to achieve the advantage after the combination. Fourier transform-ion cyclotron resonance mass spectrometry (FT-ICR MS) is characterized by its ultra high resolution and tandem mass spectrometry capabilities. Due to the collisions with neutral molecules, the image current caused by the cyclotron motion of ions in the analysis cell gradually decays under high vacuum condition. Based on this feature, the CCS of ions can be calculated by selecting an appropriate theoretical model and combining with the corresponding algorithm. This method can be directly applied to obtain the value of CCS of the selected ion through the analysis of high-resolution mass spectrometry data without increasing the cost of instrument hardware. With the development and promotion of FT-ICR MS instruments in recent years, such methods have developed rapidly in the past decade and have attracted extensive attention. There are three different ion-neutral collision models (Langevin collision model, hard-sphere collision model and energetic hard-sphere model) which have been applied to connect ion CCS and image current of FT-ICR MS instruments. Time-domain analysis, frequency-domain analysis and time-frequency analysis are three types of data analysis method used to measure ion CCS based on FT-ICR MS. Although the reliability and accuracy of the CCS data provided by such methods need to be further improved, it has been demonstrated its unique advantages in the dynamic CCS measurement and potentials in isomerization study. Such methods can be further combined with ion mobility technology to provide multi-dimensional ion structure information with the help of FT-ICR′s superior mass resolution and superior ion manipulation capabilities.

Calcified aortic valve disease (CAVD) is a common cardiovascular disease in elderly individuals. Although it was previously considered a degenerative disease, it is, in fact, a progressive disease involving multiple mechanisms. Aortic valve endothelial cells, which cover the outermost layer of the aortic valve and are directly exposed to various pathogenic factors, play a significant role in the onset and progression of CAVD. Hemodynamic changes can directly damage the structure and function of valvular endothelial cells (VECs). This leads to inflammatory infiltration and oxidative stress, which promote the progression of CAVD. VECs can regulate the pathological differentiation of valvular interstitial cells (VICs) through NO and thus affect the process of CAVD. Under the influence of pathological factors, VECs can also be transformed into VICs through EndMT, and then the pathological differentiation of VICs eventually leads to the formation of calcification. This review discusses the role of VECs, especially the role of oxidative stress in VECs, in the process of aortic valve calcification.

Objective: In this study, the finite element simulation was integrated into random vibration experiment to provide a new way of thinking about transportation packaging. Methods: The red wine inner packaging spacer, made of three different cushioning materials, was used as the research object, and the vibration response was studied through random vibration experiment. Then the finite element simulation was used to analyze the correlation between the experiment and the simulation results. Results: The results of random vibration test were highly consistent with the results of finite element simulation, and the finite element simulation was less material-and time-consuming, compared with the random test. Conclusion: The combination of random experiment and finite element method can improve the efficiency of the experiment. The finite element simulation method can provide effective guidance for the analysis and design of product inner packaging under the vibration environment of transportation.

PEDOT-Ag/AgCl is a highly promising material with dual functions of hydrogen evolution reaction (HER) and supercapacitors. In this study, a simple low-temperature stirring and light irradiation method was used to synthesize PEDOT-Ag/AgCl on the surface. Then, PEDOT-Ag/AgCl was analyzed using X-ray diffraction, scanning electron microscopy, X-ray photoelectron spectroscopy, and transmission electron microscopy. PEDOT-Ag/AgCl reacted in 1 M KOH alkaline electrolyte with an overpotential of 157 mV at 20 mA·cm−2 and a Tafel slope of 66.95 mv·dec−1. Owing to the synergistic effect of PEDOT and Ag/AgCl, this material had a small resistance (1.7 Ω) and a large specific capacitance (978 F·g−1 at current density of 0.5 A·g−1). The synthesis method can prepare nanostructured PEDOT with uniformly-distributed Ag nanoparticles and rod-shaped AgCl on the surface, which can be used as both HER electrocatalysts and supercapacitor electrodes.

Objective To investigate whether serum cystatin C (Cys C) is a potential risk factor for motoric cognitive risk syndrome (MCR) in Chinese elderly. Methods A total of 1592 elderly people were included in the subcohort from Rugao Longevity and Aging Study (RuLAS) at the third follow⁃up in 2019. The presence of subjective memory complaints was assessed by Geriatric Depression Scale (GDS⁃15) and the presence of slow walking speed was assessed by the 5⁃Meter Walking Speed Test. The presence of both symptoms was determined as MCR (n = 132). According to the serum Cys C level, the patients with serum Cys C level < 1.06 mg/L were classified as low level group (n = 794), and the patients with serum Cys C level ≥ 1.06 mg/L were classified as middle and high level group (n = 798). Univariate and multivariate stepwise Logistic regression were used to analyze whether elevated serum Cys C levels increased the risk of MCR. Results Compared with non⁃MCR group, MCR group had higher age (P = 0.041), female proportion (P = 0.027), farmer occupation (P = 0.044), married status (P = 0.034), cerebral vascular disease (P = 0.015), higher Cys C level (P = 0.023). Compared with the Cys C low level group, the Cys C middle and high level group had higher age (P = 0.000), male (P = 0.000), hypertension (P = 0.006), MCR (P = 0.049), and lower farmer proportion (P = 0.001); there was significant difference in smoking status (P = 0.041). Logistic regression analysis showed that increased Cys C level was a risk factor for the occurrence of MCR (OR = 1.419, 95%CI: 1.027-1.962; P = 0.034). Conclusions The incidence of MCR is higher in older people, female, farmers and the elderly with the history of cerebral vascular disease, and increased Cys C level is a potential risk factor for MCR.

Abstract Background With accumulating evidence suggesting that CVD has its origins in childhood obesity. The purpose of this study was to determine the effect of a real-world school-based high-intensity interval training intervention on body composition, cardiorespiratory fitness and cardiometabolic markers in obese boys aged 10 to 13 years. Methods Forty-five adolescent boys with obesity (age = 11.2 ± 0.7 years, BMI = 24.2 ± 1.0 kg/m2), were randomized to high-intensity interval training group (HIIT, n = 15), moderate-intensity continuous training group (MICT, n = 15), or a control group (CON, n = 15). The intervention groups performed three weekly exercise sessions over 12 weeks. HIIT group performed two sets of eight bouts of 15 s run at high-intensity [90 ~ 100% maximal aerobic speed (MAS)] separated by eight bouts of 15 s recovery run at low-intensity (50% MAS), MICT group performed 30 min run at moderate intensity (60 ~ 70% MAS) and CON group were instructed to continue their normal behaviors. All participants had indices of body composition, cardiorespiratory fitness (CRF) and cardiometabolic markers measured at baseline and post-intervention. Statistical differences between and within groups were determined by use of two-way analysis of variance (ANOVA) with repeated measures. Results Following the school-based training program, BMI and body fat mass decreased (BMI: − 1.8 kg/m2 vs. – 1.2 kg/m2, P

Abstract Background Pseudomembranous necrotizing laryngotracheobronchitis refers to an acute diffuse necrotizing inflammation in the mucosa of the larynx, trachea, and bronchus. It often occurs in infants and children having viral infections secondary to bacterial infections. Mycoplasma pneumoniae (M. pneumoniae) is a common pathogen that causes pneumonia in children. In recent years, serious complications due to M. pneumoniae infection, including necrotizing pneumonia, pulmonary embolism, and pleural effusion, have been increasingly reported. Case presentation An 11-year-old girl was admitted to our unit with cough, fever, and hoarseness persistent for a week. The results of the M. pneumoniae serological test, PCR examination with bronchial aspirate and bronchoalveolar lavage fluid (BALF), next-generation sequencing (mNGS) for BALF, all suggested the presence of M. pneumoniae infection. High-resolution CT scanning of the chest showed inflammation of the middle and lower lobes of the right lung. By bronchoscopy, the necrosis of the vocal cords, trachea, and bronchial mucosa was observed; each bronchial lumen contained a large amount of white viscous sputum. Pathological findings for bronchial mucosa suggested inflammatory necrosis. After administration of azithromycin and glucocorticoids, the symptoms of the patients were ameliorated. After 2 weeks post-discharge, the X-ray scan of her chest indicated the pneumonia resolution in the right lung. Conclusions In patients with pneumonia due to M. pneumoniae infection, which causes obvious hoarseness, bronchoscopy is necessary even if the lung lesions are not massively consolidated. When necrotizing lesions of the larynx, trachea, and bronchi are detected by bronchoscopy, the necrotic tissues in the corresponding parts should be conducted tissue biopsy for pathological examination. Apart from macrolide antibiotics, the administration of small doses of glucocorticoids is necessary.

This article addresses the ethical issues of respect for autonomy and informed consent, focusing on how clinicians should deal with these concepts in clinical practice. We first clarify the concept of respect for autonomy and its practical application, as well as informed consent. We then track the origin and development of informed consent both in western countries and in China. Followed by describing several challenges that limit the practice of informed consent, we make our suggestions so as to help clinicians handle the informed consent in daily practice.

To investigate the effect of substitution sites of the nitro group on the UV photodissociation (UVPD) of nitrobenzimidazole isomers, UVPD mass spectra and spectra of protonated ions of 2-nitrobenzimidazole （2-NBI）, 5-nitrobenzimidazole (5-NBI) and 7-nitrobenzimidazole (7-NBI) were studied by a Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometer combined with a tunable UV laser. Experimental results showed that the method of UVPD can distinguish the three isomers effectively. Although the UVPD spectrum of 2-NBI extended to about 360 nm, the UVPD spectra of 5-NBI and 7-NBI ended at 290 and 300 nm, respectively. As to UVPD mass spectra, the dissociation products were more diverse than the ones observed in CID experiments. And the yields of some ions significantly depended on the wavelength applied. Combined with theoretical calculations, the most important dissociation channels for the three ions were investigated in detail. For 2-NBI, two fragment ions of m/z 147 and 146 were observed, which were formed by the loss of OH• and H2O resulting from one and two H atom transfers on the N atom of imidazole ring, respectively. For 5-NBI, the process of H transfer couldn’t happen due to the long distance between the nitro group and the imidazole ring. Therefore, only fragment ions of m/z 148 by the loss of O atom were observed. For 7-NBI, its nitro group located between the benzene ring and the imidazole ring, thus only one H atom could be transferred. Therefore, only the m/z 147 (OH• loss) fragment ions could be formed in the process. The channels for the generation of fragment ions of m/z 134 and 106 were also analyzed and discussed in this paper.

BackgroundThe dysfunction of immune system and inflammation contribute to the Parkinson’s disease (PD) pathogenesis. Cytokines, oxidative stress, neurotoxin and metabolism associated enzymes participate in neuroinflammation in PD and the genes involved in them have been reported to be associated with the risk of PD. In our study, we performed a quantitative and causal analysis of the relationship between inflammatory genes and PD risk.MethodsStandard process was performed for quantitative analysis. Allele model (AM) was used as primary outcome analysis and dominant model (DM) and recessive model (RM) were applied to do the secondary analysis. Then, for those genes significantly associated with the risk of PD, we used the published GWAS summary statistics for Mendelian Randomization (MR) to test the causal analysis between them.ResultsWe included 36 variants in 18 genes for final pooled analysis. As a result, IL-6 rs1800795, TNF-α rs1799964, PON1 rs854560, CYP2D6 rs3892097, HLA-DRB rs660895, BST1 rs11931532, CCDC62 rs12817488 polymorphisms were associated with the risk of PD statistically with the ORs ranged from 0.66 to 3.19 while variants in IL-1α, IL-1β, IL-10, MnSOD, NFE2L2, CYP2E1, NOS1, NAT2, ABCB1, HFE and MTHFR were not related to the risk of PD. Besides, we observed that increasing ADP-ribosyl cyclase (coded by BST1) had causal effect on higher PD risk (OR[95%CI] =1.16[1.10-1.22]) while PON1(coded by PON1) shown probably protective effect on PD risk (OR[95%CI] =0.81[0.66-0.99]).ConclusionSeveral polymorphisms from inflammatory genes of IL-6, TNF-α, PON1, CYP2D6, HLA-DRB, BST1, CCDC62 were statistically associated with the susceptibility of PD, and with evidence of causal relationships for ADP-ribosyl cyclase and PON1 on PD risk, which may help understand the mechanisms and pathways underlying PD pathogenesis.