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The hydraulic fracturing in-situ stress testing technology was used to test two boreholes (500-meter and 520-meter deep) at the Taiyuan pumped storage power station in Shanxi Province. The in-situ stress state of critical areas was obtained, and the ground stress level, underground building layout, and lining form in the project area were analyzed. The results show that the maximum horizontal principal stress ranges from 10.98 to 18.09 MPa, the minimum horizontal principal stress from 6.79 to 11.32 MPa, and the vertical principal stress from 9.61 to 13.57 MPa. Compared with the high and low in-situ stress values at the north and south ends of Shanxi Province, respectively, the measured values are between; Compared with the simulated in-situ stress field in the Qinshui Basin, the test results are basically consistent. The vertical stress values are between the maximum horizontal principal stress values and the minimum horizontal principal stress values (SH>Sv>Sh), which means the maximum horizontal stress at the measuring point is the maximum principal stress and is in the strike-slip stress state. Its lateral pressure coefficient Kav is between 0.92 and 1.09, reflecting that the tectonic action in the engineering area is not intense. In the range of 330–506 meters, the saturated uniaxial compressive strength of the two boreholes is betwwen 35 and 107 MPa, with an average of 63.79 MPa, and the ratio of the saturated strength to the maximum principal stress (\begin{document}$ {{R}_{\mathrm{b}}} $\end{document}/σm) is between 3.54 and 5.81, belonging to the medium–high stress level. The direction of the maximum horizontal principal stress in the project area is NE 43° to NE 70.5°, and the average is NE 59.5°, consistent with the regional focal mechanism solution and GPS displacement data. From the perspective of in-situ stress orientation, the average direction of the maximum principal stress in the engineering area is NE 59.5°, and the direction of the long axis of the underground powerhouse is between NE 29.5° and NE 89.5°, which is conducive to the stability of the surrounding rock of the powerhouse. The maximum water head PH of the underground hub project is about 4.62 MPa (i.e., PH

Flowering phenology is closely related to plant reproduction, and its response to climate change is obvious; therefore, revealing its influencing factors and interspecific changes has become an important issue in global change ecology and physical geography. Previous studies have reported that interspecific changes in flowering phenology are affected by climatic factors (such as temperature and precipitation) and related to plant functional traits. However, the magnitude of the correlation between functional traits and flowering phenology varies greatly among different regions and climatic zones, and the relationship between them remains unclear. In this study, to explore whether functional traits affect interspecific changes in the flowering phenology of subtropical evergreen broadleaf species, 67 woody species in the Ningbo Botanical Garden were chosen as the study objects. Four types of flowering phenological characteristics (initial flowering time, full bloom time, falling flower time, and duration of the flowering period) were observed. General statistical analyses (i.e., maximum, minimum, mean, coefficient of variation, and frequency) were used to test the distribution patterns of flowering phenology. Four statistical methods (independent samples t-test, one-way analysis of variance, linear fitting, and generalized linear mixed effect regression models) were used to analyze the differences in flowering phenology between different types (tree vs. shrubs, different types of flower color, and fruit type) and the contributions of different functional traits (i.e., potential maximum tree height, life form, flower color, and fruit type) to the changes in flowering phenology. The results showed that: (1) the initial flowering time, full bloom time, falling flower time, and duration of the flowering period of 67 woody plants were March 28 ± 21.64 d, March 30 ± 27.02 d, April 15 ± 30.78 d, and 23.82 ± 13.31 d, respectively. (2) The initial flowering time and full bloom time of trees were significantly earlier than those of shrubs (P < 0.05); however, no significant difference was observed between them in the falling flower time or duration of the flowering period (P > 0.05). The standard deviation of the flowering phenology of trees was lower than that of shrubs, indicating that the flowering phenology of trees was more stable than that of shrubs. (3) The results of generalized linear mixed-effect regression models showed that the effects of the four functional traits on flowering phenology differed. More specifically, flower color significantly affected falling-flower time (P < 0.01). The falling flower time of colored flowers was earlier than that of white flowers. Life form and potential maximum tree height significantly affected the duration of the flowering period (P < 0.01). Plants with higher potential maximum heights have longer flowering periods. In conclusion, our study indicates that the flowering phenology of 67 woody species in the Ningbo Botanical Garden is affected by functional traits. However, the magnitude of the influence is related to the type of functional trait. Flower color and potential maximum tree height contributed more to interspecific changes in flowering phenology than other functional traits. Our results help us understand the variation in flowering phenology among woody species in subtropical regions and provide a new perspective for the theoretical exploration of phenology.

Objective To investigate the occurrence of stress depression caused by retinal cells death driven by activation of photosensitive STIM1 calcium channel and the changes in brain inflammation related to the visually-associated brain regions. Methods A mouse model of retinal injury driven by light was constructed by injecting STIM1 virus into the vitreous body of mouse eyeball. HE staining and depression-related behavior test were performed to assess the severity of retinopathy and incidence of depression. Reverse transcription-PCR (RT-PCR), Western blotting and immunofluorescence assay were used to analyze the changes of microglial cells, inflammatory factors (IL-6 and MIP-1α), nerve cell damage marker (S100B), apoptotic protein (Caspase-3) and myelin basic protein (MBP) in the depressive mice. Results ① The cell density in each layer of the mouse retina were decreased significantly under the light mode of 3 h/d for 7 consecutive days (P < 0.05). ② In the open field test, the times of entering the central area, moving distance in the central area and total distance of movement were obviously decreased (P < 0.05), the index of sucrose preference of the sucrose preference test was significantly decreased (P < 0.01), and the immobile time in the forced swimming test and the tail suspension test was notably increased (P < 0.001) in the mice from the photosensitive group. The positive rate of depression in all the 4 tests (open field test, sucrose preference test, forced swimming test and tail suspension test) was 23.26%. ③The density of IBa-1+ cells in the visual cortex and dorsolateral geniculate nucleus was elevated in the depressive mice of the photosensitive group (P < 0.05). The expression of IL-6 and MIP-1α at mRNA level was obviously elevated (P < 0.000 1), as well as the expression of S100B and Caspase-3 at protein level was significantly enhanced (P < 0.001), while the MBP protein in the white matter was clearly weakened (P < 0.05). The intensity of MBP fluorescence signal was evidently reduced in the corpus callosum (cc) area and cingulate gyrus (cg) area in white matter (P < 0.000 1). Conclusion A stress depression-like mouse model caused by light-driven retinal injury is successfully constructed. Pathogenesis of depression is closely associated with inflammatory environment and neuronal apoptosis in the vision-related brain regions and demyelinating injury in white matter.

Few therapeutic drugs and increased drug resistance have aggravated the current treatment difficulties of Cryptococcus in recent years. To better understand the antifungal drug resistance mechanism and treatment strategy of cryptococcosis. In this review, by combining the fundamental features of Cryptococcus reproduction leading to changes in its genome, we review recent research into the mechanism of four current anti-cryptococcal agents, coupled with new therapeutic strategies and the application of advanced technologies WGS and CRISPR-Cas9 in this field, hoping to provide a broad idea for the future clinical therapy of cryptococcosis.

China’s construction of high-speed rail has accomplished outstanding achievements that have drawn attention from around the world and have played a significant role in driving economic and social development. To investigate the reasons behind the competition between neighboring cities in the region during the process of setting up a high-speed railroad station, the economic principles of high-speed railway construction and operation station setup and its role effect are analyzed using the theories of investment multiplier, spatial spillover, and pole-axis. This is intended to solve the mystery surrounding the competition for the station between neighboring regions and offer theoretical support for the science of high-speed railway station setup. The result of the study demonstrates that the construction and operation of high-speed railway has significant spatial spillover effects that effectively enhance local employment levels and promote the coordinated development of the regional economy along the routes, in addition to having multiplier effects on national income that cause the regional economy to grow exponentially.

Stuttering is a disorder that affects about 1% of the population and manifests as speech disfluencies. Reading difficulties and disabilities are commonly found in this population. Nonetheless, speech disfluencies have not been explored in adult struggling readers (ASRs). In the current study, we examined the rate of stuttering in ASRs as well as the relationships between their speech fluency and reading skills. A total of 120 participants were interviewed about their experiences with reading and administered standardized reading and reading-related assessments. Speech fluency and the criterion for stuttering were based on the interview. About 18.3% of the sample met the criterion for stuttering. ASRs who stutter (ASRs-S) and ASRs who do not stutter (ASRs-NS) did not differ in their reading and reading-related skills. ASRs-S had higher rates of negative correlations between reading and reading-related skills compared with ASRs-NS. Correlation patterns between performance on standardized assessments point to higher rates of uneven skills or dissociations in ASRs-S. These findings may have implications for the assessment and instruction for ASRs. [For the corresponding grantee submission, see ED620471.]

Since 1975, researchers have conducted interventions to improve the word-problem performance of elementary school students facing mathematics difficulties. The current study reports a meta-analysis of 52 studies that examined the effect of these interventions. We estimated multivariate, random-effects models (REM) with robust variance estimation (RVE) with and without outliers. Results showed a large, positive, and significant mean weighted effect size (g = 1.01 for the model with outliers; g = 0.81 for the model without outliers). Findings of meta-regression analyses showed several moderators, such as sample composition, group size, intervention dosage, group assignment approach, interventionist, year of publication, and dependent measure type, significantly explained heterogeneity in effects across studies. A sensitivity analysis showed these results were generally robust to outliers. We offer possible explanations for the findings and discuss study limitations. Finally, we propose recommendations for future research and classroom practice.