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In addition to addressing the labor shortage due to an aging population, the transition to autonomous vehicle (AV)-based mobility services offers enhanced efficiency and operational flexibility for public transportation. However, much of the existing focus has been on improving AV safety without fully considering road conditions and real-world service demand. This study contributes to the literature by proposing a comprehensive framework for efficiently integrating AV-based mobility services at the network level, addressing these gaps. The framework analyzes and optimizes service networks by incorporating actual demand patterns, quantifying road segment difficulty from an AV perspective, and developing an optimization model based on these factors. The framework begins by quantifying the operational difficulty of road segments through an evaluation of Operational Design Domains (ODDs), providing a precise measure of AV suitability under varying road conditions. It then introduces a quantitative metric to assess operational feasibility, considering factors such as the service margin, costs, and safety risks. Using these metrics alongside Genetic Algorithms (GAs), the framework identifies an optimal service network that balances safety, efficiency, and profitability. By analyzing real-world data from different mobility services, such as taxis, Demand-Responsive Transport (DRT), and Special Transportation Services (STSs), this study highlights the need for service-specific strategies to optimize AV deployment. The findings show that optimal networks vary with demand patterns and road difficulty, demonstrating the importance of tailored network designs. This research provides a scalable, data-driven approach for integrating AV services into public transportation systems and lays the foundation for further improvements by incorporating dynamic factors and broader urban contexts. [ABSTRACT FROM AUTHOR]