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Abstract In mammals, the production of mature oocytes necessitates rigorous regulation of the discontinuous meiotic cell-cycle progression at both the transcriptional and post-transcriptional levels. However, the factors underlying this sophisticated but explicit process remain largely unclear. Here we characterize the function of N-acetyltransferase 10 (Nat10), a writer for N4-acetylcytidine (ac4C) on RNA molecules, in mouse oocyte development. We provide genetic evidence that Nat10 is essential for oocyte meiotic prophase I progression, oocyte growth and maturation by sculpting the maternal transcriptome through timely degradation of poly(A) tail mRNAs. This is achieved through the ac4C deposition on the key CCR4-NOT complex transcripts. Importantly, we devise a method for examining the poly(A) tail length (PAT), termed Hairpin Adaptor-poly(A) tail length (HA-PAT), which outperforms conventional methods in terms of cost, sensitivity, and efficiency. In summary, these findings provide genetic evidence that unveils the indispensable role of maternal Nat10 in oocyte development.

It is of great significance to explore the physical properties of optical propagation, thus the scholars are keen on the physical essence represented by classical mathematical physics equations. In this paper, a higher order nonlinear Schrödinger equation describing the behavior of polarization mode in optical fibers is firstly analyzed qualitatively, and the existence of periodic and soliton solutions is proved by using bifurcation method. Secondly, all chirped wave patterns with special form for the equation are obtained by using the complete discrimination system for polynomial method and direct integral method, the chirped rational functions and some elliptic functions wave patterns are initially found. As a result, the parameter stability of these patterns is given for the first time, which shows the variant of patterns as the parameters change, and the graphs of several typical patterns are drawn.

Abstract Heating furnace is an important energy‐consuming equipment in oilfield. In order to evaluate the operation status of heating furnace more accurately, in this paper, the production process of heating furnace in an oilfield is studied by numerical simulation technology, and the specific reasons affecting its thermal efficiency are deeply explored. First, the numerical simulation model of oilfield heating furnace production process is established, which is to digitize the geometric structure of the heating furnace, study the characteristics of the geometric shape, and generate the appropriate calculation grid. Second, numerical simulation and analysis are carried out for the working process of the heating furnace under rated and nonrated conditions, respectively. The medium flow characteristics, flame shape, temperature distribution, and pipe temperature distribution in the heating furnace under different conditions are systematically discussed. Finally, through the simulation analysis, it is found that the burner rotating device is beneficial to strengthen the heat transfer, and the change of load will affect the efficiency of the heating furnace. Under the rated working condition, design exhaust temperature is 260℃, numerical result is 225.8℃, and the error is −13.2%; design export temperature of water is 60℃, numerical result is 58.1℃, and the error is −3.2%; design temperature difference between inlet and outlet of water is 30℃, numerical result is 28.1℃, and the error is −6.3%. The working process of oilfield heating furnace is a complex coupling heat transfer process of combustion, flow, and heat transfer. The numerical results show that the working process of oilfield heating furnace could be successfully realized by means of numerical simulation. Under variable load conditions, in order to ensure that the export temperature of water is close to design temperature, it is necessary to change the fuel quantity in equal proportion to the water quantity. It is hoped that the reported results provide interesting information among the oilfield heating furnace operators.

Summary: Germline development is challenging to study due to the diversity of cell types in mammalian testis. Here, we present an optimized protocol, namely centrifugal elutriation, that allows the simultaneous isolation of mouse germ cells at different stages with high purity within ∼2 h. This approach exploits the JE-5.0 centrifugal elutriation system that fractionates cells based on differential sedimentation gravity. We herein provide the optimized parameters and procedures for isolation of elongating spermatids, round spermatids, and pachytene spermatocytes from adult mouse testes.For complete details on the use and execution of this protocol, please refer to Bao et al. (2018). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

ObjectiveThis study aimed to investigate the clinical efficacy of a balloon compression day-surgery operation under the guidance of spiral computed tomography (CT) three-dimensional (3D) reconstruction for the treatment of trigeminal neuralgia.MethodsThe clinical efficacy and related indexes of 380 patients with trigeminal neuralgia treated by a spiral CT-guided balloon compression day-surgery operation in the pain department of Beijing TianTan Hospital, from October 2017 to March 2021, were retrospectively analyzed.ResultsFive patients failed due to foramen ovale puncture or in placing the balloon; two patients had ineffective results after the operation and re-entered the hospital for secondary balloon compression. The initial effective rate of the operation in the 380 patients was 98.16%. All patients were discharged on the day of the operation, the average operation time was 26.46 ± 12.15 min, and the average interval from the completion of the operation to discharge was 2.67 ± 0.95 h. During the follow-up period (1–41 months), 12 patients had pain recurrence, and a Kaplan–Meier analysis revealed that the cumulative pain-free recurrence survival rate at 41 months after the operation was 80.64%. No complications related to foramen ovale puncture occurred.ConclusionThe spiral CT-guided balloon compression day-surgery operation is safe, effective, and worthy of clinical promotion.

Emotions are one of the pillars of all human beings which can play a vital role in providing education. Emotions can affect all aspects of education. The feeling of creativity is one of the subsets of emotions. This feeling strongly affects the performance of education and the level of involvement of students. Student involvement has different aspects: social aspect; individual aspect, and emotional aspect. The present review shows that the emotional aspect of L2 engagement plays a pivotal role in the process of learning the language in English as a foreign language (EFL) and English as a second language (ESL) context. In dealing with the emotional aspect of teachers, the personal, social, and environmental aspects of the individual should be considered. The paper concludes with some pedagogical implications and provides some suggestions for future research.

The switch machine, an essential element of railway infrastructure, is crucial in maintaining the safety of railway operations. Traditional methods for fault diagnosis are constrained by their dependence on extensive labeled datasets. Semi-supervised learning (SSL), although a promising solution to the scarcity of samples, faces challenges such as the imbalance of pseudo-labels and inadequate data representation. In response, this paper presents the Semi-Supervised Adaptive Matrix Machine (SAMM) model, designed for the fault diagnosis of switch machine. SAMM amalgamates semi-supervised learning with adaptive technologies, leveraging adaptive low-rank regularizer to discern the fundamental links between the rows and columns of matrix data and applying adaptive penalty items to correct imbalances across sample categories. This model methodically enlarges its labeled dataset using probabilistic outputs and semi-supervised, automatically adjusting parameters to accommodate diverse data distributions and structural nuances. The SAMM model's optimization process employs the alternating direction method of multipliers (ADMM) to identify solutions efficiently. Experimental evidence from a dataset containing current signals from switch machines indicates that SAMM outperforms existing baseline models, demonstrating its exceptional status diagnostic capabilities in situations where labeled samples are scarce. Consequently, SAMM offers an innovative and effective approach to semi-supervised classification tasks involving matrix data. [ABSTRACT FROM AUTHOR]

Background: Patients infected with Acinetobacter baumannii (AB) bacteremia in hospital have high morbidity and mortality. We analyzed the clinical characteristics of pneumonia and nonpneumonia-related AB bloodstream infections (AB BSIs) and explored the possible independent risk factors for the incidence and prognosis of pneumonia-related AB BSIs. Methods: A retrospective monocentric observational study was performed. All 117 episodes of hospital-acquired AB bacteremia sorted into groups of pneumonia-related AB BSIs (n = 45) and nonpneumonia-related AB BSIs (n = 72) were eligible. Univariate/multivariate logistic regression analysis was used to explore the independent risk factors. The primary outcome was the antibiotic susceptibility in vitro of pneumonia-related AB BSIs group. The secondary outcome was the independent risk factor for the pneumonia-related AB BSIs group. Results: Among 117 patients with AB BSIs, the pneumonia-related group had a greater risk of multidrug resistant A. baumannii (MDRAB) infection (84.44%) and carbapenem-resistant A. baumannii (CRAB) infection (80%). Polymyxin, minocycline and amikacin had relatively high susceptibility rates (> 80%) in the nonpneumonia-related group. However, in the pneumonia-related group, only polymyxin had a drug susceptibility rate of over 80%. Univariate analysis showed that survival time (day), CRAB, MDRAB, length of hospital stay prior to culture, length of ICU stay prior to culture, immunocompromised status, antibiotics used prior to culture (n > = 3 types), endotracheal tube, fiberoptic bronchoscopy, PITT, SOFA and invasive interventions (n > = 3 types) were associated with pneumonia-related AB bacteremia. The multivariate logistic regression analysis revealed that recent surgery (within 1 mo) [P = 0.043; 0.306 (0.098–0.962)] and invasive interventions (n > = 3 types) [P = 0.021; 0.072 (0.008–0.671)] were independent risk factors related to pneumonia-related AB bacteremia. Multivariate logistic regression analysis revealed that length of ICU stay prior to culture [P = 0.009; 0.959 (0.930–0.990)] and recent surgery (within 1 mo) [P = 0.004; 0.260 (0.105–0.646)] were independent risk factors for mortality in patients with pneumonia-related AB bacteremia. The Kaplan‒Meier curve and the timing test showed that patients with pneumonia-related AB bacteremia had shorter survival time compared to those with nonpneumonia-related AB bacteremia. Conclusions: Our study found that A. baumannii had a high rate of antibiotic resistance in vitro in the pneumonia-related bacteremia group, and was only sensitive to polymyxin. Recent surgery was a significantly independent predictor in patients with pneumonia-related AB bacteremia. [ABSTRACT FROM AUTHOR]

In the research on the new generation of intelligent rail transit system, the prevention of train collision accidents is still a focus. Virtual coupling technology controls the running speed and interval distance of two or more trains cooperatively through Train-to-Train (T2T) direct communication technology. The aim is to form a coordinated logical system and jointly complete the train control and dispatching. In this paper, a virtual coupling train formation control method based on the model predictive control (MPC) framework is proposed. From the establishment of the virtual coupling system, the construction of an MPC model and decoupling method, how to integrate virtual coupling technology into the rail transit train collision protection is studied. The safety and reliability of the system are calculated through fault tree and the method of constructing reliability function. The research shows that if virtual coupling technology is used for train collision protection, the overall safety and reliability of the train control system is improved. [ABSTRACT FROM AUTHOR]