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The problems of uneven load and low operating efficiency in the oil-gathering system of old oilfields lead to higher operating costs. In order to reduce operating costs, the layout-reconfiguration optimization model is established, and the minimum comprehensive investment is taken as the objective function. The multi-constraint conditions, such as the current situation of the oil-gathering system, the processing capacity, the possibility of pipeline failure, and the obstacles, are considered. The hybrid arithmetic–fireworks optimization algorithm (AFOA) is proposed to solve the model. Combined with the experience of the hybrid metaheuristic algorithm, using hybrid metaheuristics, the hybrid of the arithmetic optimization algorithm (AOA) and the operator of the fireworks algorithm (FWA) is considered, and some improved operators of FWA are integrated into AOA to form a new algorithm (AFOA) to achieve a better solution effect. Compared with the 11 other algorithms, AFOA has better solution efficiency. This method is applied to the actual case of an old oilfield. The optimized scheme increases the average load rate of the station by 15.9% and reduces the operating costs by 38.1% per year. Overall, the reconstruction costs will be recovered in a short period.

Abstract In mammals, the production of mature oocytes necessitates rigorous regulation of the discontinuous meiotic cell-cycle progression at both the transcriptional and post-transcriptional levels. However, the factors underlying this sophisticated but explicit process remain largely unclear. Here we characterize the function of N-acetyltransferase 10 (Nat10), a writer for N4-acetylcytidine (ac4C) on RNA molecules, in mouse oocyte development. We provide genetic evidence that Nat10 is essential for oocyte meiotic prophase I progression, oocyte growth and maturation by sculpting the maternal transcriptome through timely degradation of poly(A) tail mRNAs. This is achieved through the ac4C deposition on the key CCR4-NOT complex transcripts. Importantly, we devise a method for examining the poly(A) tail length (PAT), termed Hairpin Adaptor-poly(A) tail length (HA-PAT), which outperforms conventional methods in terms of cost, sensitivity, and efficiency. In summary, these findings provide genetic evidence that unveils the indispensable role of maternal Nat10 in oocyte development.

Abstract Heating furnace is an important energy‐consuming equipment in oilfield. In order to evaluate the operation status of heating furnace more accurately, in this paper, the production process of heating furnace in an oilfield is studied by numerical simulation technology, and the specific reasons affecting its thermal efficiency are deeply explored. First, the numerical simulation model of oilfield heating furnace production process is established, which is to digitize the geometric structure of the heating furnace, study the characteristics of the geometric shape, and generate the appropriate calculation grid. Second, numerical simulation and analysis are carried out for the working process of the heating furnace under rated and nonrated conditions, respectively. The medium flow characteristics, flame shape, temperature distribution, and pipe temperature distribution in the heating furnace under different conditions are systematically discussed. Finally, through the simulation analysis, it is found that the burner rotating device is beneficial to strengthen the heat transfer, and the change of load will affect the efficiency of the heating furnace. Under the rated working condition, design exhaust temperature is 260℃, numerical result is 225.8℃, and the error is −13.2%; design export temperature of water is 60℃, numerical result is 58.1℃, and the error is −3.2%; design temperature difference between inlet and outlet of water is 30℃, numerical result is 28.1℃, and the error is −6.3%. The working process of oilfield heating furnace is a complex coupling heat transfer process of combustion, flow, and heat transfer. The numerical results show that the working process of oilfield heating furnace could be successfully realized by means of numerical simulation. Under variable load conditions, in order to ensure that the export temperature of water is close to design temperature, it is necessary to change the fuel quantity in equal proportion to the water quantity. It is hoped that the reported results provide interesting information among the oilfield heating furnace operators.

It is of great significance to explore the physical properties of optical propagation, thus the scholars are keen on the physical essence represented by classical mathematical physics equations. In this paper, a higher order nonlinear Schrödinger equation describing the behavior of polarization mode in optical fibers is firstly analyzed qualitatively, and the existence of periodic and soliton solutions is proved by using bifurcation method. Secondly, all chirped wave patterns with special form for the equation are obtained by using the complete discrimination system for polynomial method and direct integral method, the chirped rational functions and some elliptic functions wave patterns are initially found. As a result, the parameter stability of these patterns is given for the first time, which shows the variant of patterns as the parameters change, and the graphs of several typical patterns are drawn.

Summary: Germline development is challenging to study due to the diversity of cell types in mammalian testis. Here, we present an optimized protocol, namely centrifugal elutriation, that allows the simultaneous isolation of mouse germ cells at different stages with high purity within ∼2 h. This approach exploits the JE-5.0 centrifugal elutriation system that fractionates cells based on differential sedimentation gravity. We herein provide the optimized parameters and procedures for isolation of elongating spermatids, round spermatids, and pachytene spermatocytes from adult mouse testes.For complete details on the use and execution of this protocol, please refer to Bao et al. (2018). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

ObjectiveThis study aimed to investigate the clinical efficacy of a balloon compression day-surgery operation under the guidance of spiral computed tomography (CT) three-dimensional (3D) reconstruction for the treatment of trigeminal neuralgia.MethodsThe clinical efficacy and related indexes of 380 patients with trigeminal neuralgia treated by a spiral CT-guided balloon compression day-surgery operation in the pain department of Beijing TianTan Hospital, from October 2017 to March 2021, were retrospectively analyzed.ResultsFive patients failed due to foramen ovale puncture or in placing the balloon; two patients had ineffective results after the operation and re-entered the hospital for secondary balloon compression. The initial effective rate of the operation in the 380 patients was 98.16%. All patients were discharged on the day of the operation, the average operation time was 26.46 ± 12.15 min, and the average interval from the completion of the operation to discharge was 2.67 ± 0.95 h. During the follow-up period (1–41 months), 12 patients had pain recurrence, and a Kaplan–Meier analysis revealed that the cumulative pain-free recurrence survival rate at 41 months after the operation was 80.64%. No complications related to foramen ovale puncture occurred.ConclusionThe spiral CT-guided balloon compression day-surgery operation is safe, effective, and worthy of clinical promotion.

Photocatalytic technology has received widespread attention as an effective means of treating organic pollutants in wastewater, but the development of effective and sustainable photocatalysts remains a serious challenge. In this study, a novel Mn0.2Cd0.8S (MCS)/Bi4O5Br2 (BOB) heterojunction photocatalyst was successfully prepared by a two-step solvothermal method. The photocatalytic activity of the MCS/BOB heterojunction photocatalyst for degrading Rhodamine B (RhB) is significantly enhanced relative to Mn0.2Cd0.8S and Bi4O5Br2. When the molar content of Mn0.2Cd0.8S was 15% of Bi4O5Br2, the prepared 15% MCS/BOB had the best photocatalytic performance toward RhB with the degradation rate of 91.7% under 60 min of visible light irradiation. The apparent rate constant of 15% MCS/BOB is 10 and 7 times higher than that of Mn0.2Cd0.8S and Bi4O5Br2, respectively. The excellent photocatalytic performance is associated with the increased specific surface area, extended light absorption range, and proper construction of Z-scheme heterojunction that facilitate effective charge separation and excellent redox capacity. In addition, the heterojunction photocatalyst shows remarkable photocatalytic stability. This study constructs a novel and effective Z-scheme heterojunction photocatalyst and promotes the exploration of photocatalyst in the purification of organic wastewater. [ABSTRACT FROM AUTHOR]

ABSTRACT Iron plays critical roles in many cellular functions, including energy production, metabolism, and cell proliferation. However, the role of iron in maintaining oocyte quality remains unclear. In this study, DMT1 was identified as a key iron transporter during porcine oocyte maturation. The results demonstrated that iron deficiency in porcine oocyte led to aberrant meiotic progression, accompanied by increased gene expression of DMT1. Inhibition of DMT1 resulted in the failure of cumulus cell expansion and oocyte maturation, along by the abnormal actin and microtubule assembly. Furthermore, loss of DMT1 function caused disruption in mitochondrial function and dynamics, resulting in oxidative stress and Ca2+ dyshomeostasis. Additionally, the absence of DMT1 function activated PINK1/Parkin‐dependent mitophagy in porcine oocyte. These findings suggested that DMT1 played a crucial role in safeguarding oocyte quality by protecting against iron‐deficiency‐induced mitochondrial dysfunction and autophagy. This study provided compelling evidence that DMT1 and iron homeostasis were crucial for maintaining the capacity of porcine oocyte maturation. Moreover, the results hinted at the potential of DMT1 as a novel therapeutic target for treating iron deficiency‐related female reproductive disorders. [ABSTRACT FROM AUTHOR]