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Your search keyword '"Kuwahara, Mizu"' showing total 9 results
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9 results on '"Kuwahara, Mizu"'

3. Anti-CD80/86 antibodies inhibit inflammatory reaction and improve graft survival in a high-risk murine corneal transplantation rejection model

Author

Zhu, Jun, Inomata, Takenori, Nakamura, Masahiro, Fujimoto, Keiichi, Akasaki, Yasutsugu, Fujio, Kenta, Yanagawa, Ai, Uchida, Koichiro, Sung, Jaemyoung, Negishi, Naoko, Nagino, Ken, Okumura, Yuichi, Miura, Maria, Shokirova, Hurramhon, Kuwahara, Mizu, Hirosawa, Kunihiko, Midorikawa-Inomata, Akie, Eguchi, Atsuko, Huang, Tianxiang, Yagita, Hideo, Habu, Sonoko, Okumura, Ko, and Murakami, Akira

Published
2022
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4. Biological effects of stored platelet-rich plasma eye-drops in corneal wound healing

Author

Okumura, Yuichi, Inomata, Takenori, Fujimoto, Keiichi, Fujio, Kenta, Zhu, Jun, Yanagawa, Ai, Shokirova, Hurramhon, Saita, Yoshitomo, Kobayashi, Yohei, Nagao, Masahi, Nishio, Hirofumi, Sung, Jaemyoung, Midorikawa-Inomata, Akie, Eguchi, Atsuko, Nagino, Ken, Akasaki, Yasutsugu, Hirosawa, Kunihiko, Huang, Tianxiang, Kuwahara, Mizu, and Murakami, Akira

Abstract

Background/aimsThis study aimed to assess the efficacy and sterility of stored platelet-rich plasma (PRP) eye-drops for corneal epithelial wound healing compared with those of autologous serum (AS) eye-drops.MethodsAt our single institution, PRP and AS eye-drops were prepared using peripheral blood obtained from six healthy volunteers and stored at 4°C. Platelet and leucocyte counts and transforming growth factor (TGF)-β1, epidermal growth factor (EGF), and fibronectin levels were assessed during storage for up to 4 weeks. Sterility was assessed by culturing 4-week poststorage samples. PRP, AS, and phosphate-buffered saline (PBS) eye-drop efficacies were compared using corneal epithelial wound healing assays in vitroand in vivoand monitoring wound areas under a microscope every 3 hours.ResultsHigher platelet and lower leucocyte counts were seen in PRP than in whole blood on the day of preparation. After storage, TGF-β1, EGF, and fibronectin levels were significantly higher in PRP than in AS eye-drops. In vitroand in vivo, PRP eye-drops used on the day of preparation significantly promoted corneal epithelial wound healing compared with PBS. Moreover, PRP eye-drops stored for 4 weeks significantly promoted corneal wound healing compared with PBS and AS eye-drops.ConclusionPRP eye-drops stored at 4°C for 4 weeks promoted corneal epithelial wound healing with higher levels of growth factors than those observed in AS eye-drops, while maintaining sterility, suggesting that this preparation satisfies the unmet medical needs in the treatment of refractory keratoconjunctival epithelial disorders.

Published
2024
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5. Biological effects of stored platelet-rich plasma eye-drops in corneal wound healing

Author

Okumura, Yuichi, primary, Inomata, Takenori, additional, Fujimoto, Keiichi, additional, Fujio, Kenta, additional, Zhu, Jun, additional, Yanagawa, Ai, additional, Shokirova, Hurramhon, additional, Saita, Yoshitomo, additional, Kobayashi, Yohei, additional, Nagao, Masahi, additional, Nishio, Hirofumi, additional, Sung, Jaemyoung, additional, Midorikawa-Inomata, Akie, additional, Eguchi, Atsuko, additional, Nagino, Ken, additional, Akasaki, Yasutsugu, additional, Hirosawa, Kunihiko, additional, Huang, Tianxiang, additional, Kuwahara, Mizu, additional, and Murakami, Akira, additional

Published
2022
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6. Characteristics and Clinical Ocular Manifestations in Patients with Acute Corneal Graft Rejection after Receiving the COVID-19 Vaccine: A Systematic Review

Author

Fujio, Kenta, primary, Sung, Jaemyoung, additional, Nakatani, Satoru, additional, Yamamoto, Kazuko, additional, Iwagami, Masao, additional, Fujimoto, Keiichi, additional, Shokirova, Hurramhon, additional, Okumura, Yuichi, additional, Akasaki, Yasutsugu, additional, Nagino, Ken, additional, Midorikawa-Inomata, Akie, additional, Hirosawa, Kunihiko, additional, Miura, Maria, additional, Huang, Tianxiang, additional, Morooka, Yuki, additional, Kuwahara, Mizu, additional, Murakami, Akira, additional, and Inomata, Takenori, additional

Published
2022
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7. Prevalence of Comorbidity between Dry Eye and Allergic Conjunctivitis: A Systematic Review and Meta-Analysis

Author

Akasaki, Yasutsugu, primary, Inomata, Takenori, additional, Sung, Jaemyoung, additional, Nakamura, Masahiro, additional, Kitazawa, Koji, additional, Shih, Kendrick Co, additional, Adachi, Takeya, additional, Okumura, Yuichi, additional, Fujio, Kenta, additional, Nagino, Ken, additional, Midorikawa-Inomata, Akie, additional, Kuwahara, Mizu, additional, Hirosawa, Kunihiko, additional, Huang, Tianxiang, additional, Morooka, Yuki, additional, Shokirova, Hurramhon, additional, Eguchi, Atsuko, additional, and Murakami, Akira, additional

Published
2022
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8. Application of Animal Models in Interpreting Dry Eye Disease

Author

Zhu, Jun, primary, Inomata, Takenori, additional, Shih, Kendrick Co, additional, Okumura, Yuichi, additional, Fujio, Kenta, additional, Huang, Tianxiang, additional, Nagino, Ken, additional, Akasaki, Yasutsugu, additional, Fujimoto, Keiichi, additional, Yanagawa, Ai, additional, Miura, Maria, additional, Midorikawa-Inomata, Akie, additional, Hirosawa, Kunihiko, additional, Kuwahara, Mizu, additional, Shokirova, Hurramhon, additional, Eguchi, Atsuko, additional, Morooka, Yuki, additional, Chen, Fang, additional, and Murakami, Akira, additional

Published
2022
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9. Biological effects of stored platelet-rich plasma eye-drops in corneal wound healing.

Author

Okumura Y, Inomata T, Fujimoto K, Fujio K, Zhu J, Yanagawa A, Shokirova H, Saita Y, Kobayashi Y, Nagao M, Nishio H, Sung J, Midorikawa-Inomata A, Eguchi A, Nagino K, Akasaki Y, Hirosawa K, Huang T, Kuwahara M, and Murakami A

Subjects
Humans, Epidermal Growth Factor metabolism, Epidermal Growth Factor pharmacology, Fibronectins metabolism, Ophthalmic Solutions, Cell Proliferation, Wound Healing, Corneal Injuries metabolism, Platelet-Rich Plasma metabolism, Infertility metabolism
Abstract

Background/aims: This study aimed to assess the efficacy and sterility of stored platelet-rich plasma (PRP) eye-drops for corneal epithelial wound healing compared with those of autologous serum (AS) eye-drops., Methods: At our single institution, PRP and AS eye-drops were prepared using peripheral blood obtained from six healthy volunteers and stored at 4°C. Platelet and leucocyte counts and transforming growth factor (TGF)-β1, epidermal growth factor (EGF), and fibronectin levels were assessed during storage for up to 4 weeks. Sterility was assessed by culturing 4-week poststorage samples. PRP, AS, and phosphate-buffered saline (PBS) eye-drop efficacies were compared using corneal epithelial wound healing assays in vitro and in vivo and monitoring wound areas under a microscope every 3 hours., Results: Higher platelet and lower leucocyte counts were seen in PRP than in whole blood on the day of preparation. After storage, TGF-β1, EGF, and fibronectin levels were significantly higher in PRP than in AS eye-drops. In vitro and in vivo , PRP eye-drops used on the day of preparation significantly promoted corneal epithelial wound healing compared with PBS. Moreover, PRP eye-drops stored for 4 weeks significantly promoted corneal wound healing compared with PBS and AS eye-drops., Conclusion: PRP eye-drops stored at 4°C for 4 weeks promoted corneal epithelial wound healing with higher levels of growth factors than those observed in AS eye-drops, while maintaining sterility, suggesting that this preparation satisfies the unmet medical needs in the treatment of refractory keratoconjunctival epithelial disorders., Competing Interests: Competing interests: YO, TI,and AM have a patent pending for the manufacturing method of PRP eye-drops (pending patent application 2020-164360)., (© Author(s) (or their employer(s)) 2024. No commercial re-use. See rights and permissions. Published by BMJ.)

Published
2023
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