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Cold stress is a potent abiotic stress resulting in cotton yield and fiber quality loss. We hypothesized that GhGTG1 could increase low temperature stress tolerance by improving the sensitivity to ABA. Genome-wide identification, biochemical traits and functional characterization of GhGTG1 , were investigated under different levels of ABA and cold stress. GhGTG1 had two highly conserved functional domains (ABA_GPCR, GPHR_N) in all cotton species. Under ABA treatment, over expression of GhGTG1 in Arabidopsis had a lower germination rate and root elongation. While under 4 ℃ stress, transgenic plants had a higher germination rate and root elongation. This indicate that GhGTG1 improved sensitivity to ABA under cold stress. In transgenic plants, CAT and SOD activity was increased, while H 2 O 2 and MDA content decreased. Less trypan blue and DAB staining area suggested that GhGTG1 enhances cold stress resistance. Under 4 ℃ treatment, silence cotton seedlings through VIGS method, resulted in a poor growth, lower relative leaf water content, chlorophyll content but a higher ion leakage and excised leaf water loss. The activity of CAT and SOD decreased, H 2 O 2 and MDA content was increased. Two marker genes (OST1 and ABCG40) are associated with the stomatal movement, but these genes were substantially suppressed. This suggesting that GhGTG1 can induce cold stress tolerance by improving ABA sensitivity Via., maintaining stomatal movement. These data will provide new insight of cold resistance mechanism in cotton and further will help the breeder to develop new cotton cold stress tolerance germplasm. [Display omitted] • GhGTG1 increased ABA sensitivity to improve cold stress tolerance in Arabidopsis. • GhGTG1 can improve cold tolerance by regulating stomata opening and closure through ABA pathway in cotton. • GhGTG1 can induce cold stress tolerance by improving ABA sensitivity to control the stomatal movement. [ABSTRACT FROM AUTHOR]