Search

Your search keyword '"Klas Karre"' showing total 9 results
Start Over Author "Klas Karre" Publication Year Range Last 3 years
9 results on '"Klas Karre"'

1. Supplementary Table 4. from Accumulation of Circulating CCR7+ Natural Killer Cells Marks Melanoma Evolution and Reveals a CCL19-Dependent Metastatic Pathway

Author

Ennio Carbone, Matilde Todaro, Paolo A. Ascierto, Klas Karre, Alessandro Moretta, Aroldo Rizzo, Elio Gulletta, Francesco Saverio Costanzo, Valter Agosti, Genny Del Zotto, Silvia Pesce, Emanuela Marcenaro, Rossana Tallerico, Antonio M. Grimaldi, Emilia Dora Giovannone, Cinzia Garofalo, Domenico Mallardo, Gabriele Madonna, Mariaelena Capone, Tiziana Apuzzo, Valeria Ventura, Alice Turdo, and Costanza Maria Cristiani

Abstract

Univariate analysis of biomarkers identified by OPLS-DA.

Published
2023

2. Supplementary Table 3. from Accumulation of Circulating CCR7+ Natural Killer Cells Marks Melanoma Evolution and Reveals a CCL19-Dependent Metastatic Pathway

Author

Ennio Carbone, Matilde Todaro, Paolo A. Ascierto, Klas Karre, Alessandro Moretta, Aroldo Rizzo, Elio Gulletta, Francesco Saverio Costanzo, Valter Agosti, Genny Del Zotto, Silvia Pesce, Emanuela Marcenaro, Rossana Tallerico, Antonio M. Grimaldi, Emilia Dora Giovannone, Cinzia Garofalo, Domenico Mallardo, Gabriele Madonna, Mariaelena Capone, Tiziana Apuzzo, Valeria Ventura, Alice Turdo, and Costanza Maria Cristiani

Abstract

Immune, biological and clinical variables used to built OPLS-DA model

Published
2023

3. Supplementary Table 1. from Accumulation of Circulating CCR7+ Natural Killer Cells Marks Melanoma Evolution and Reveals a CCL19-Dependent Metastatic Pathway

Author

Ennio Carbone, Matilde Todaro, Paolo A. Ascierto, Klas Karre, Alessandro Moretta, Aroldo Rizzo, Elio Gulletta, Francesco Saverio Costanzo, Valter Agosti, Genny Del Zotto, Silvia Pesce, Emanuela Marcenaro, Rossana Tallerico, Antonio M. Grimaldi, Emilia Dora Giovannone, Cinzia Garofalo, Domenico Mallardo, Gabriele Madonna, Mariaelena Capone, Tiziana Apuzzo, Valeria Ventura, Alice Turdo, and Costanza Maria Cristiani

Abstract

Primary melanoma cell lines

Published
2023

4. Supplementary Table 2. from Accumulation of Circulating CCR7+ Natural Killer Cells Marks Melanoma Evolution and Reveals a CCL19-Dependent Metastatic Pathway

Author

Ennio Carbone, Matilde Todaro, Paolo A. Ascierto, Klas Karre, Alessandro Moretta, Aroldo Rizzo, Elio Gulletta, Francesco Saverio Costanzo, Valter Agosti, Genny Del Zotto, Silvia Pesce, Emanuela Marcenaro, Rossana Tallerico, Antonio M. Grimaldi, Emilia Dora Giovannone, Cinzia Garofalo, Domenico Mallardo, Gabriele Madonna, Mariaelena Capone, Tiziana Apuzzo, Valeria Ventura, Alice Turdo, and Costanza Maria Cristiani

Abstract

Antibodies used for flow cytometry analysis

Published
2023

5. Data from Accumulation of Circulating CCR7+ Natural Killer Cells Marks Melanoma Evolution and Reveals a CCL19-Dependent Metastatic Pathway

Author

Ennio Carbone, Matilde Todaro, Paolo A. Ascierto, Klas Karre, Alessandro Moretta, Aroldo Rizzo, Elio Gulletta, Francesco Saverio Costanzo, Valter Agosti, Genny Del Zotto, Silvia Pesce, Emanuela Marcenaro, Rossana Tallerico, Antonio M. Grimaldi, Emilia Dora Giovannone, Cinzia Garofalo, Domenico Mallardo, Gabriele Madonna, Mariaelena Capone, Tiziana Apuzzo, Valeria Ventura, Alice Turdo, and Costanza Maria Cristiani

Abstract

Immune checkpoint blockade therapy has changed prognoses for many melanoma patients. However, immune responses that correlate with clinical progression of the disease are still poorly understood. To identify immune responses correlating with melanoma clinical evolution, we analyzed serum cytokines as well as circulating NK and T-cell subpopulations from melanoma patients. The patients' immune profiles suggested that melanoma progression leads to changes in peripheral blood NK and T-cell subsets. Stage IV melanoma was characterized by an increased frequency of CCR7+CD56bright NK cells as well as high serum concentrations of the CCR7 ligand CCL19. CCR7 expression and CCL19 secretion were also observed in melanoma cell lines. The CCR7+ melanoma cell subpopulation coexpressed PD-L1 and Galectin-9 and had stemness properties. Analysis of melanoma-derived cancer stem cells (CSC) showed high CCR7 expression; these CSCs were efficiently recognized and killed by NK cells. An accumulation of CCR7+, PD-L1+, and Galectin-9+ melanoma cells in melanoma metastases was demonstrated ex vivo. Altogether, our data identify biomarkers that may mark a CCR7-driven metastatic melanoma pathway.

Published
2023

6. Supplementary Figure 1. Immunomodulatory ligands on melanoma cells. from Accumulation of Circulating CCR7+ Natural Killer Cells Marks Melanoma Evolution and Reveals a CCL19-Dependent Metastatic Pathway

Author

Ennio Carbone, Matilde Todaro, Paolo A. Ascierto, Klas Karre, Alessandro Moretta, Aroldo Rizzo, Elio Gulletta, Francesco Saverio Costanzo, Valter Agosti, Genny Del Zotto, Silvia Pesce, Emanuela Marcenaro, Rossana Tallerico, Antonio M. Grimaldi, Emilia Dora Giovannone, Cinzia Garofalo, Domenico Mallardo, Gabriele Madonna, Mariaelena Capone, Tiziana Apuzzo, Valeria Ventura, Alice Turdo, and Costanza Maria Cristiani

Abstract

Frequency and expression of the indicated molecules on CCR7- and CCR7+ melanoma cells.

Published
2023

7. Overall survival of patients stratified for CCL19 serum concentration. from Accumulation of Circulating CCR7+ Natural Killer Cells Marks Melanoma Evolution and Reveals a CCL19-Dependent Metastatic Pathway

Author

Ennio Carbone, Matilde Todaro, Paolo A. Ascierto, Klas Karre, Alessandro Moretta, Aroldo Rizzo, Elio Gulletta, Francesco Saverio Costanzo, Valter Agosti, Genny Del Zotto, Silvia Pesce, Emanuela Marcenaro, Rossana Tallerico, Antonio M. Grimaldi, Emilia Dora Giovannone, Cinzia Garofalo, Domenico Mallardo, Gabriele Madonna, Mariaelena Capone, Tiziana Apuzzo, Valeria Ventura, Alice Turdo, and Costanza Maria Cristiani

Abstract

Kaplan-Meier survival curves in 9 patients with low and 13 with high CCL19 serum concentration.

Published
2023

8. CSCs characterization. from Accumulation of Circulating CCR7+ Natural Killer Cells Marks Melanoma Evolution and Reveals a CCL19-Dependent Metastatic Pathway

Author

Ennio Carbone, Matilde Todaro, Paolo A. Ascierto, Klas Karre, Alessandro Moretta, Aroldo Rizzo, Elio Gulletta, Francesco Saverio Costanzo, Valter Agosti, Genny Del Zotto, Silvia Pesce, Emanuela Marcenaro, Rossana Tallerico, Antonio M. Grimaldi, Emilia Dora Giovannone, Cinzia Garofalo, Domenico Mallardo, Gabriele Madonna, Mariaelena Capone, Tiziana Apuzzo, Valeria Ventura, Alice Turdo, and Costanza Maria Cristiani

Abstract

Negative controls for CD44, CD271, ABCB5, and CD166 antibodies on melanoma CSCs.

Published
2023

9. PD-1 expression on mouse intratumoral NK cells and its effects on NK cell phenotype

Author

Arnika K. Wagner, Nadir Kadri, Chris Tibbitt, Koen van de Ven, Sunitha Bagawath-Singh, Denys Oliinyk, Eric LeGresley, Nicole Campbell, Stephanie Trittel, Peggy Riese, Ulf Ribacke, Tatyana Sandalova, Adnane Achour, Klas Kärre, and Benedict J. Chambers

Subjects
Immunology, Components of the immune system, Cancer, Transcriptomics, Science
Abstract

Summary: Although PD-1 was shown to be a hallmark of T cells exhaustion, controversial studies have been reported on the role of PD-1 on NK cells. Here, we found by flow cytometry and single cell RNA sequencing analysis that PD-1 can be expressed on MHC class I-deficient tumor-infiltrating NK cells in vivo. We also demonstrate distinct alterations in the phenotype of PD-1-deficient NK cells and a more mature phenotype which might reduce their capacity to migrate and kill in vivo. Tumor-infiltrating NK cells that express PD-1 were highly associated with the expression of CXCR6. Furthermore, our results demonstrate that PD-L1 molecules in membranes of PD-1-deficient NK cells migrate faster than in NK cells from wild-type mice, suggesting that PD-1 and PD-L1 form cis interactions with each other on NK cells. These data demonstrate that there may be a role for the PD-1/PD-L1 axis in tumor-infiltrating NK cells in vivo.

Published
2022
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results