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Your search keyword '"KishoreKumar Reddy"' showing total 3 results
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3 results on '"KishoreKumar Reddy"'

1. Functional characterization of a new ORF βV1 encoded by radish leaf curl betasatellite

Author

Neha Gupta, Kishorekumar Reddy, Prabu Gnanasekaran, Ying Zhai, Supriya Chakraborty, and Hanu R. Pappu

Subjects
begomovirus, radish leaf curl betasatellite, hypersensitive response, βV1, reactive oxygen species, tomato leaf curl New Delhi virus, Plant culture, SB1-1110
Abstract

Whitefly-transmitted begomoviruses infect and damage a wide range of food, feed, and fiber crops worldwide. Some of these viruses are associated with betasatellite molecules that are known to enhance viral pathogenesis. In this study, we investigated the function of a novel βV1 protein encoded by radish leaf curl betasatellite (RaLCB) by overexpressing the protein using potato virus X (PVX)-based virus vector in Nicotiana benthamiana. βV1 protein induced lesions on leaves, suggestive of hypersensitive response (HR), indicating cell death. The HR reaction induced by βV1 protein was accompanied by an increased accumulation of reactive oxygen species (ROS), free radicals, and HR-related transcripts. Subcellular localization through confocal microscopy revealed that βV1 protein localizes to the cellular periphery. βV1 was also found to interact with replication enhancer protein (AC3) of helper virus in the nucleus. The current findings suggest that βV1 functions as a protein elicitor and a pathogenicity determinant.

Published
2022
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2. Rice transcriptome upon infection with Xanthomonas oryzae pv. oryzae relative to its avirulent T3SS-defective strain exposed modulation of many stress responsive genes

Author

Kalyan K. Mondal, Aditya Kulshreshtha, Pratap J. Handique, Debashis Borbora, Yuvika Rajrana, Geeta Verma, Ankita Bhattacharya, Aarzoo Qamar, Amrutha Lakshmi, KishoreKumar Reddy, Madhvi Soni, Thungri Ghoshal, E. R. Rashmi, S. Mrutyunjaya, N. S. Kalaivanan, and Chander Mani

Subjects
Xanthomonas oryzae pv. oryzae, Xanthomonas oryzae pv, Environmental Science (miscellaneous), Agricultural and Biological Sciences (miscellaneous), Type III secretion system, oryzae, Infectious Diseases, Differentially expressed genes, Genetics, 2.1 Biological and endogenous factors, Original Article, Biochemistry and Cell Biology, Aetiology, Infection, Bacterial blight, Biotechnology
Abstract

Xanthomonas oryzae pv. oryzae (Xoo) is a destructive pathogen that causes bacterial blight disease of rice worldwide. Xoo uses T3SS (type III secretion system) effectors to subvert rice innate immunity. However, the comprehensive knowledge of rice genes involved in T3SS effectors-mediated interaction remains unclear. In this study, the transcriptome profiles of rice infected with a virulent Xoo strain from North-eastern region of India relatives to its avirulent strain (that lacks functional T3SS) were analyzed at early (2–6 hpi) and late (16–24 hpi) hours of infection. Out of total 255 differentially expressed genes (DEGs), during early infection, 62 and 70 genes were upregulated and downregulated, respectively. At late infection, 70 and 53 genes were upregulated and downregulated, respectively. The transcriptomic data identified many differentially expressed resistant genes, transposons, transcription factors, serine/threonine protein kinase, cytochrome P450 and peroxidase genes that are involved in plant defense. Pathway analysis revealed that these DEGs are involved in hormone signaling, plant defense, cellular metabolism, growth and development processes. DEGs associated with plant defense were also validated through quantitative real-time PCR. Our study brings a comprehensive picture of the rice genes that are being differentially expressed during bacterial blight infection. Nevertheless, the DEG-associated pathways would provide sensible targets for developing resistance to bacterial blight. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s13205-022-03193-4.

Published
2022

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