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Simple Summary: Livestock herding is an essential and time-honored practice in Balochistan, playing a pivotal role in the region's economy, culture, and way of life. The livestock sector is of utmost important in the province, catering to nearly 20% of the national stock. Over centuries, large predators and their prey species, including livestock, have coexisted in these mountainous landscapes. Consequently, large carnivores are more likely to interact with humans due to their extensive home ranges. This research aims to explore the impact of livestock depredation by large predators on livelihoods and conservation efforts in two districts of Balochistan, Pakistan. A human–carnivore conflict survey was conducted from July to September 2019, gathering data from 311 residents in the selected study area. Large predators in the region preyed on a total of 876 livestock over a year, comprising 560 goats, 292 sheep, 19 cows, and 5 donkeys. The gray wolf emerged as the primary predator, accounting for 66.3% of livestock depredation, which was followed by the caracal (24.3%), Asiatic jackal (8.9%), and striped hyena (0.6%). The economic loss totaled USD 78,694. Notably, 80% of respondents held negative perceptions toward wolves compared to 24.4% for caracals. Only 20.6% of respondents were aware of the importance of conserving carnivores. Livestock depredation by carnivores has fostered negative perceptions among locals toward these animals. There exists a lack of awareness regarding the significance of conserving carnivore species and their ecological roles. It is crucial to raise awareness among communities about the ecological importance of predators like the gray wolf, caracal, Asiatic jackal, striped hyena, and Balochistan black bear through community meetings and educational seminars. Furthermore, providing basic education to herders on effective livestock guarding practices is recommended to mitigate human–carnivore conflicts and promote coexistence between wildlife and local communities in Balochistan. Livestock herding is a vital practice in Balochistan, contributing to the economy and culture. The livestock sector is significant in Balochistan, providing 20% of the national stock. Large predators and their prey species, including livestock, have coexisted in these mountainous landscapes for centuries. The aim of the present research is to investigate the impacts of livestock depredation by large predators on livelihoods and predator conservation in two districts of Balochistan, Pakistan. A human–carnivore conflict survey was conducted from July to September 2019, collecting data from 311 residents in a selected study area. Large predators in the study area preyed on a total of 876 livestock during a one-year period, including 560 goats, 292 sheep, 19 cows, and 5 donkeys. The gray wolf is the leading predator, responsible for 66.3% of livestock depredation, followed by the caracal (24.3%), Asiatic jackal (8.9%), and striped hyena (0.6%). The total economic loss was USD 78,694. Overall, 80% of respondents had a negative perception of wolves compared to 24.4% for caracals. Only 20.6% of respondents knew about the importance of conserving carnivores. Livestock depredation by carnivores in the study area created a negative perception of these animals among people. There is a lack of awareness about the importance of conserving carnivore species and their role in the ecosystem. This lack of understanding has ultimately led to detrimental effects on predator populations. It is imperative to raise awareness among people about the ecological significance of carnivores through community meetings, seminars in educational institutions, and providing basic education to herders about effective livestock guarding practices. [ABSTRACT FROM AUTHOR]

The article considers several generations of Pakistani immigrants from Karachi to various cities in the United States such as Chicago and New York and explores how chronic diseases are shaped by the reverberations of conflict in kin relations in the US and Pakistan. Instead of examining the experience of chronic illness in isolation, the article considers how webs of evolving relations, tensions, competition, and conflicts among distant and close kin are experienced by the vulnerable and those who try to care for them. These conflicts are shaped by the experience of absence and also in the lives of intimate kin, and often appear in violent form. In navigating these relations, caregivers of those with illnesses develop strategies to maintain communications with wider kinship networks while also shielding the sick from the ramifications of ongoing disputes. [ABSTRACT FROM AUTHOR]

Diclofenac sodium was extensively used for treating arthritis, osteoarthritis and skeletal muscular injuries, which ultimately caused troubles for aquatic organisms as well as human beings. In this study, homogeneous catalytic advanced oxidation processes, including Fe2+/persulfate, Fe2+/peroxymonosulfate and Fe2+/H2O2, were used for the degradation of diclofenac sodium in water, without using UV-C light. About 89, 82 and 54% DCF sodium was decomposed by Fe2+/persulfate, Fe2+/peroxymonosulfate and Fe2+/H2O2, respectively, in 60 min. The degradation of diclofenac sodium followed the pseudo first-order kinetics, in all cases. The degradation efficiency of diclofenac sodium was significantly affected in the presence of various anions, such as NO3−, HCO3− and SO42−. The mineralization studies revealed 62, 45 and 32% total carbon removal by Fe2+/persulfate, Fe2+/peroxymonosulfate and Fe2+/H2O2, respectively, in 60 min. In addition, the degradation byproducts of diclofenac sodium were determined by FTIR analysis. The results revealed that the Fe2+/oxidant system, particularly Fe2+/persulfate, was a promising technology for the elimination of toxic pharmaceuticals, such as diclofenac sodium, from the water environment. [ABSTRACT FROM AUTHOR]

The removal of water pollutants by photocatalysis is a promising technique, mainly due to its environmentally friendly and sustainable nature. In this study, the degradation of a recalcitrant organic pollutant, malachite green (MG), was investigated in water by a microstructured silver oxide photocatalyst. The silver oxide (Ag2O) microparticles (MPs) were synthesized by a low-cost, green method, mediated by green tea leaves extract. The surface, morphological and optical properties of the synthesized Ag2O MPs were determined by scanning electron microscopy (SEM), energy dispersive X-ray (EDX) analysis, X-ray diffraction (XRD) analysis, Fourier transform infrared (FTIR) spectroscopy, and ultraviolet-visible (UV-visible) spectrophotometry. The synthesized Ag2O MPs showed good photoactivity, represented by 83% degradation of malachite green (MG) ([C]0 = 0.4 mM, Ag2O loading = 0.1 g L−1) at neutral pH, in 3 h. Persulfate ions (PS) showed a strong synergistic effect on the efficiency of solar/Ag2O photocatalysis, represented by complete MG removal in 15 min, in the presence of 1.6 mM PS. The results revealed that solar/Ag2O, particularly solar/Ag2O/PS photocatalysis is a promising method for the elimination of toxic organic pollutants, such as malachite green, from the water environment. [ABSTRACT FROM AUTHOR]

The Helicobacter pylori have ancestral relation with the family of Proteobacteria, order Campylobacterales, family Helicobacteraceae. It is the first and most important cause of gastric carcinoma along with peptic ulcer and dyspepsia carcinoma. H. pylori is also responsible for mucosa associated with lymphoid tissue (MALT) and Known Hodgkin Lymphoma. It is assessed that patients having H. pylori are a 30% to 40% possibility of evolving gastric ulcer and a 10% to 20% possibility of developing distal gastric cancer. It causes chronic inflammation and significantly increases the risk of duodenal, gastric ulcer, and cancer. It is the second leading cause of cancer-related deaths in the world. In the current study, a total of 210 gastric biopsy specimens were collected from the patient with a history of gastritis from Chughtai Lab in 10% NBF. All tissue size was measured and gross examined these samples were processed in an automated tissue processor Tissue-Tek VIP VI (Japan). After processing, the embedding of tissues was done in paraffin wax at Tissue-Tek TEC. 2- 3 µm sections were prepared using rotary microtome Leica 201 USA. A comparison was carried out between H&E and IHC. Helicobacter pylori were detected in 88 cases out of 200 while 09 samples were either poorly preserved or autolyzed that's why further processing and diagnosis were not possible. One case was diagnosed as poorly differentiated adenocarcinoma; the sample was collected from a female patient of 56 years. Out of 88 positive cases, 66 (75%) were initially screened as positive for H.pyloriby H&E staining due to the presence of mild or moderate colonization while the remaining 25% cases were negative by H&E staining but with suspicion of neutrophils and lymphoid aggregates. All 88 cases were further confirmed by IHC. Our findings confirmed that IHC is a batter reliable and more accurate method in the diagnosis of H.pylori and it should be considered the gold standard. [ABSTRACT FROM AUTHOR]

Indian squash (Praecitrullus fistulosus) crop faces heat and drought during its growth that is considered the most important abiotic stress in semi-arid areas. Seed priming with growth regulators enhances stress tolerance; hence, mitigates the adverse effects of unpredictable stresses due to adverse weather conditions. This two-year (2019 and 2020) study was conducted to infer the role of seed priming in improving heat tolerance of Indian squash (cultivar Sahavi) through improvement in physiological and antioxidant defense systems. Six treatments that included no priming (control), hydropriming, priming with indole acetic acid (IAA) at 100 mg L-1, salicylic acid (SA) at 50 mg L-1, ascorbic acid (AA) at 100 mg L-1 and thiourea at 500 mg L-1 each for 06 hours) were included in the study. Results revealed that priming with AA and SA significantly (P ≤ 0.05) enhanced germination (39 and 47%), germination index (57 and 58%), plant height (23 and 22%), vine length (15 and 14%), number of fruits per plant (62%), fruit weight per plant (66 and 67%), economic yield (32%), photosynthesis rate (18 and 17%), protein content (10%), proline (23%), glycine betaine (3%), malondialdehyde content (11 and 10%) and catalase activity (24%) compared to control treatment. Furthermore, seed priming with AA and SA significantly (P ≤ 0.05) shortened the mean germination time (25 and 28%) compared to the control. The results indicated that AA and SA had significant potential to mitigate adverse effects of heat stress in Indian squash. Findings from this study showed that seed priming with AA and SA promoted heat-stress tolerance and enhanced growth and productivity of Indian squash. [ABSTRACT FROM AUTHOR]

Interferon/Ribavirin therapy has been replaced by Direct Acting Antivirals (DAAs) due to emergence of Resistance Associated Variants (RAVs) and decrease Sustain Virologic Response (SVR). Current study investigated treatment response of Sofosbuvir and Ribavirin in chronic HCV patients. Total 256 HCV patients with genotype 1a, 2 and 3a received sofosbuvir/ribavirin according to international standards. HCV RNA presence in serum was used as marker for end treatment response (ETR) and sustain virologic response after 24 weeks of treatment (SVR24) in each case. Response to treatment with SOF + RBV was found statistically significant among different HCV genotypes (GT) as out of 47 HCV GT1 patients 42(89.36%) resulted into good ETR but 4(9.52%) of these relapsed and 5(10.63%) led into virologic failure. 5(100%) HCV GT2 patients resulted into SVR24 whereas, out of 204 HCV GT3 patients 194(95.69%) achieved good ETR however, 8(4.12%) of these relapsed and 10(4.90%) resulted in to virologic failure. Efficacy of therapy was found non-significant in treatment naïve and treatment experienced patients as in this study out of 145 treatment naïve patients 139(95.86%) achieved good ETR where 4(2.87%) relapsed while 6(4.13%) led into virologic break through on the other hand among 111 treatment experienced patients 102(91.89%) resulted into good ETR but 8(7.84%) relapsed whereas 9(8.10%) lead into virologic failure. Current study also propose that various liver and spleen complications/liver cirrhosis are related to response of HCV patients to SOF + RBV therapy whereas, variables like old age, gender is not compromising treatment response to DAAs therapy. Various mild side effects encountered by patients during treatment were fatigue, insomnia, headache, nausea, burning body, diarrhea, cough. Overall, this study reported 89.45% efficacy of SOF + RBV regime in chronic HCV Pakistani patients. Current study suggests hunting for possible reasons of resistance so that SOF + RBV therapy may not share the same fortune as previous therapies in near future. [ABSTRACT FROM AUTHOR]