46 results on '"Kaye, M."'
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2. An Application of Queueing Theory on the Ticketing Booth of Light Rail Transit 1 (LRT-1) Central Station.
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Eunice Gabrielle A. Reyes, Gerome Kaye M. Cangco, Shiela Mae C. Ilagan, Hazel P. Pacunayen, Jeunise A. Piamonte, and Josephine D. German
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- 2023
- Full Text
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3. A Comparison on the Usability of Mobile e-Wallet Applications: GCash, Maya, Grabpay.
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Ma. Janice J. Gumasing, Gerome Kaye M. Cangco, Shiela Mae C. Ilagan, and Eunice Gabrielle A. Reyes
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- 2023
- Full Text
- View/download PDF
4. Microplastics in surface water of Laguna de Bay: first documented evidence on the largest lake in the Philippines
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Arcadio, Cris Gel Loui A., Navarro, Carl Kenneth P., Similatan, Kaye M., Inocente, Sherley Ann T., Ancla, Sheila Mae B., Banda, Marybeth Hope T., Capangpangan, Rey Y., Torres, Armi G., and Bacosa, Hernando P.
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- 2023
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5. Retrospective review of recent ASA prescribing practices for primary prevention of major adverse cardiovascular events in the Canadian Fabry Disease Initiative cohort
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Théberge, Emilie, primary, Selvage, Caroline, additional, LeMoine, Kaye M., additional, Robichaud, Rebecca, additional, Zhou, Lily, additional, Yeung, Darwin, additional, West, Michael L., additional, Sirrs, Sandra, additional, and Lehman, Anna, additional
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- 2024
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6. Chest pain subtype prevalence in the British Columbia cohort of the Canadian Fabry Disease Initiative
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Théberge, Emilie, primary, Selvage, Caroline, additional, LeMoine, Kaye M., additional, Robichaud, Rebecca, additional, West, Michael L., additional, Sirrs, Sandra, additional, Sedlak, Tara, additional, Yeung, Darwin, additional, and Lehman, Anna, additional
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- 2024
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7. Evaluation of the WDVCAS Hearing Support Pilot
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Wangmann, J, Kaye, M, Thomas, K, Wangmann, J, Kaye, M, and Thomas, K
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This report sets out the findings of the independent evaluation of the Women’s Domestic Violence Court Advocacy Service (WDVCAS) Hearing Support Pilot Program (the ‘Pilot’). In August 2022 the NSW and Commonwealth governments announced funding under the National Partnership Agreement on Family, Domestic and Sexual Violence Responses 2021-23, for a 12-month pilot to provide non-legal court support for women who are victims in domestic and family violence (DFV) matters that proceed to a defended hearing in the Local Court of NSW. This includes hearings for criminal charges and/or Apprehended Domestic Violence Order (ADVO) matters. The funding for the Pilot is administered by Legal Aid NSW through the Women’s Domestic Violence Court Advocacy Program (WDVCAP). The Pilot commenced in November 2022 and initially funded 14 of the 27 WDVCASs covering 73 courts. After the Evaluation commenced, the Pilot was extended for a further 12 months and expanded to every WDVCAS (from November 2023). The Evaluation focuses on the original 14 Pilot sites, with the remaining 13 sites referred to as ‘non-Pilot sites’ in this report. The Evaluation was commissioned by Legal Aid NSW to assess whether the Pilot was implemented as intended, whether and to what extent it achieved its objectives, and provide advice on whether the Pilot should be continued and/or expanded.
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- 2024
8. The Help-Seeking Behavior of Male College Students in Kabankalan Catholic College.
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Javilla, Roy B., Pineda, Jewel M., Quinto, Leah Grace B., Roque, Ma. Christine S., Vergara, Rile M., Vinson, Kisha Kaye M., Degillo, Jose Leonardo L., and Paglomutan, Paul Melcar C.
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HELP-seeking behavior ,MALE college students ,CATHOLIC universities & colleges ,PSYCHOLOGICAL tests ,ACADEMIC departments ,SCHOOL year - Abstract
This study is intended to understand extent of help-seeking behavior of male college students. This study used descriptive quantitative research design. The participants were the male college students of the College Department of Kabankalan Catholic College, enrolled in the Academic Year 2022 - 2023. Moreover, this study used Attitude Toward Seeking Professional Psychological Help Test, a standardized instrument measuring the help-seeking behavior of the participants. The results revealed that male college students opt to seek psychological help when they are psychologically distressed. Moreover, it was also found out that the perception towards seeking help in matters pertaining to the time needed for the process hinders the participants to seek help. In addition, help-seeking inside the institution is free and readily accessible, yet the data gathered shown that participants still thought that to seek help may cost them a lot. Awareness for access to where the participants can seek help could be a great factor of this occurrence. Thus, the study suggests that further investigation discussing the factors of help-seeking behavior of certain population is significant to shed light on this phenomenon. Furthermore, this study highly recommends to all institutions to initiate programs and projects that will help combat the misconception of seeking help to the appropriate professionals in order to continuously promote accessible psychological help to all. [ABSTRACT FROM AUTHOR]
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- 2024
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9. Microplastic ingestion by adult milkfish Chanos chanos (Forsskål, 1775) in aquaculture system: The case of Butuan Bay, Philippines
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Similatan, Kaye M., primary, Arcadio, Cris Gel Loui A., additional, Navarro, Carl Kenneth P., additional, Capangpangan, Rey Y., additional, and Bacosa, Hernando P., additional
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- 2023
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10. From Surface Water to the Deep Sea: A Review on Factors Affecting the Biodegradation of Spilled Oil in Marine Environment
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Hernando Pactao Bacosa, Sheila Mae B. Ancla, Cris Gel Loui A. Arcadio, John Russel A. Dalogdog, Dioniela Mae C. Ellos, Heather Dale A. Hayag, Jiza Gay P. Jarabe, Ahl Jimhar T. Karim, Carl Kenneth P. Navarro, Mae Princess I. Palma, Rodolfo A. Romarate, Kaye M. Similatan, Jude Albert B. Tangkion, Shann Neil A. Yurong, Jhonamie A. Mabuhay-Omar, Chihiro Inoue, and Puspa L. Adhikari
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oil spills ,oil weathering ,bioremediation ,polycyclic aromatic hydrocarbons (PAHs) ,fates of spilled oil ,environment ,Naval architecture. Shipbuilding. Marine engineering ,VM1-989 ,Oceanography ,GC1-1581 - Abstract
Over the past century, the demand for petroleum products has increased rapidly, leading to higher oil extraction, processing and transportation, which result in numerous oil spills in coastal-marine environments. As the spilled oil can negatively affect the coastal-marine ecosystems, its transport and fates captured a significant interest of the scientific community and regulatory agencies. Typically, the environment has natural mechanisms (e.g., photooxidation, biodegradation, evaporation) to weather/degrade and remove the spilled oil from the environment. Among various oil weathering mechanisms, biodegradation by naturally occurring bacterial populations removes a majority of spilled oil, thus the focus on bioremediation has increased significantly. Helping in the marginal recognition of this promising technique for oil-spill degradation, this paper reviews recently published articles that will help broaden the understanding of the factors affecting biodegradation of spilled oil in coastal-marine environments. The goal of this review is to examine the effects of various environmental variables that contribute to oil degradation in the coastal-marine environments, as well as the factors that influence these processes. Physico-chemical parameters such as temperature, oxygen level, pressure, shoreline energy, salinity, and pH are taken into account. In general, increase in temperature, exposure to sunlight (photooxidation), dissolved oxygen (DO), nutrients (nitrogen, phosphorous and potassium), shoreline energy (physical advection—waves) and diverse hydrocarbon-degrading microorganisms consortium were found to increase spilled oil degradation in marine environments. In contrast, higher initial oil concentration and seawater pressure can lower oil degradation rates. There is limited information on the influences of seawater pH and salinity on oil degradation, thus warranting additional research. This comprehensive review can be used as a guide for bioremediation modeling and mitigating future oil spill pollution in the marine environment by utilizing the bacteria adapted to certain conditions.
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- 2022
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11. Ziritaxestat, a novel autotaxin inhibitor, and lung function in idiopathic pulmonary fibrosis
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Maher, TM, Ford, P, Brown, KK, Costabel, U, Cottin, V, Danoff, SK, Groenveld, I, Helmer, E, Jenkins, RG, Milner, J, Molenberghs, G, Penninckx, B, Randall, MJ, Van Den Blink, B, Fieuw, A, Vandenrijn, C, Rocak, S, Seghers, I, Shao, L, Taneja, A, Jentsch, G, Watkins, TR, Wuyts, WA, Kreuter, M, Verbruggen, N, Prasad, N, Wijsenbeek, MS, Chambers, D, Chia, M, Corte, T, Glaspole, I, Goh, N, Holmes, M, Malouf, M, Thien, F, Veitch, E, Bondue, B, Dahlqvist, C, Froidure, A, Slabbynck, H, Wuyts, W, Cartagena Salinas, C, Feijoó Seoane, R, Martínez, V, Maturana, R, Pavie Gallegos, J, Rosenblut, A, Silva, R, Undurraga Pereira, A, Doubkova, M, Pauk, N, Plackova, M, Sterclova, M, Bendstrup, E, Shaker, SB, Titlestad, I, Budweiser, S, Grohé, C, Koschel, D, Prasse, A, Weber, M, Wirtz, H, Antoniou, K, Daniil, Z, Gaga, M, Papakosta, D, Izumi, S, Okamoto, M, Guerreros Benavides, A, Iberico Barrera, C, Peña Villalobos, AM, Campo Ezquibela, A, Cifrian Martinez, JM, Fernandez Fabrellas, E, Leiro, V, Molina-Molina, M, Nieto Barbero, A, Sellares Torres, J, Valenzuela, C, Cheng, S-L, Kuo, P-H, Lee, K-Y, Sheu, C-C, Gunen, H, Mogulkoc Bishop, N, Nayci, S, Adamali, H, Bianchi, S, Chaudhuri, N, Gibbons, M, Hart, S, Molyneaux, P, Parfrey, H, Saini, G, Spencer, LG, Wiscombe, S, Antin-Ozerkis, D, Bascom, R, Belperio, J, Britt, E, Fitzgerald, J, Gomez Manjarres, D, Gotfried, M, Gupta, N, Hotchkin, D, Kaye, M, Kreider, M, Kureishy, S, Lacamera, P, Lancaster, L, Lasky, J, Lorch, D, Mannem, H, Morrow, L, Moua, T, Nambiar, A, Raghu, G, Raj, R, Ramaswamy, M, Reddy, R, Russell, T, Scholand, MB, Shea, B, Suliman, S, Swigris, J, Thavarajah, K, Tolle, L, Tomic, R, Warshoff, N, Wesselius, L, Yung, G, Bergna, M, De Salvo, M, Fernandez Acquier, M, Rodriguez, A, Saez Scherbovsky, P, Assayag, D, Dhar, A, Khalil, N, Morisset, J, Provencher, S, Ryerson, C, Shapera, S, Bourdin, A, Crestani, B, Lebargy, F, Reynaud-Gaubert, M, Bonella, FT, Claussen, M, Hammerl, P, Karagiannidis, C, Keller, C, Randerath, W, Stubbe, B, Csánky, E, Medgyasszay, B, Muller, V, Adir, Y, Bar-Shai, A, Berkman, N, Fink, G, Kramer, M, Shitrit, D, Bargagli, E, Gasparini, S, Harari, S, Ravaglia, C, Richeldi, L, Vancheri, C, Ebina, M, Fujita, M, Ichikado, K, Inoue, Y, Ishikawa, N, Kato, M, Kawamura, T, Kondoh, Y, Nishioka, Y, Ogura, T, Owan, I, Saito, T, Sakamoto, N, Sakamoto, K, Shirai, M, Suda, T, Tomii, K, Chung, MP, Jeong, SH, Park, CS, Park, JS, Song, JW, Uh, S-T, Chavarria Martinez, U, Montano Gonzalez, E, Ramirez, A, Selman Lama, ME, Bresser, P, Kramer, H, Mostard, R, Nossent, E, Veltkamp, M, Wijsenbeek, M, Beckert, L, Chang, CL, Veale, A, Wilsher, M, Bednarek, M, Gasior, G, Jasieniak-Pinis, G, Jassem, E, Mroz, R, Piotrowski, W, Abdullah, I, Ambaram, A, Irusen, E, Van der Linden, M, Van Zyl-Smit, R, Williams, P, Allen, J, Averill, F, Belloli, E, Brown, A, Case, A, Chaudhary, S, Criner, G, DeBoer, K, Dilling, D, Dorf, J, Enelow, R, Ettinger, N, Feldman, J, Gibson, K, Golden, J, Hamblin, M, Hunninghake, G, Karunakara, R, Kim, H, Luckhardt, T, Menon, P, Morrison, L, Oldham, J, Patel, N, Schmidt, S, Strek, M, Summer, R, Sussman, R, Tita, J, Veeraraghavan, S, Whelan, T, and Zibrak, J
- Abstract
Importance There is a major need for effective, well-tolerated treatments for idiopathic pulmonary fibrosis (IPF). Objective To assess the efficacy and safety of the autotaxin inhibitor ziritaxestat in patients with IPF. Design, Setting, and Participants The 2 identically designed, phase 3, randomized clinical trials, ISABELA 1 and ISABELA 2, were conducted in Africa, Asia-Pacific region, Europe, Latin America, the Middle East, and North America (26 countries). A total of 1306 patients with IPF were randomized (525 patients at 106 sites in ISABELA 1 and 781 patients at 121 sites in ISABELA 2). Enrollment began in November 2018 in both trials and follow-up was completed early due to study termination on April 12, 2021, for ISABELA 1 and on March 30, 2021, for ISABELA 2. Interventions Patients were randomized 1:1:1 to receive 600 mg of oral ziritaxestat, 200 mg of ziritaxestat, or placebo once daily in addition to local standard of care (pirfenidone, nintedanib, or neither) for at least 52 weeks. Main Outcomes and Measures The primary outcome was the annual rate of decline for forced vital capacity (FVC) at week 52. The key secondary outcomes were disease progression, time to first respiratory-related hospitalization, and change from baseline in St George’s Respiratory Questionnaire total score (range, 0 to 100; higher scores indicate poorer health-related quality of life). Results At the time of study termination, 525 patients were randomized in ISABELA 1 and 781 patients in ISABELA 2 (mean age: 70.0 [SD, 7.2] years in ISABELA 1 and 69.8 [SD, 7.1] years in ISABELA 2; male: 82.4% and 81.2%, respectively). The trials were terminated early after an independent data and safety monitoring committee concluded that the benefit to risk profile of ziritaxestat no longer supported their continuation. Ziritaxestat did not improve the annual rate of FVC decline vs placebo in either study. In ISABELA 1, the least-squares mean annual rate of FVC decline was –124.6 mL (95% CI, −178.0 to −71.2 mL) with 600 mg of ziritaxestat vs –147.3 mL (95% CI, −199.8 to −94.7 mL) with placebo (between-group difference, 22.7 mL [95% CI, −52.3 to 97.6 mL]), and –173.9 mL (95% CI, −225.7 to −122.2 mL) with 200 mg of ziritaxestat (between-group difference vs placebo, −26.7 mL [95% CI, −100.5 to 47.1 mL]). In ISABELA 2, the least-squares mean annual rate of FVC decline was –173.8 mL (95% CI, −209.2 to −138.4 mL) with 600 mg of ziritaxestat vs –176.6 mL (95% CI, −211.4 to −141.8 mL) with placebo (between-group difference, 2.8 mL [95% CI, −46.9 to 52.4 mL]) and –174.9 mL (95% CI, −209.5 to −140.2 mL) with 200 mg of ziritaxestat (between-group difference vs placebo, 1.7 mL [95% CI, −47.4 to 50.8 mL]). There was no benefit with ziritaxestat vs placebo for the key secondary outcomes. In ISABELA 1, all-cause mortality was 8.0% with 600 mg of ziritaxestat, 4.6% with 200 mg of ziritaxestat, and 6.3% with placebo; in ISABELA 2, it was 9.3% with 600 mg of ziritaxestat, 8.5% with 200 mg of ziritaxestat, and 4.7% with placebo. Conclusions and Relevance Ziritaxestat did not improve clinical outcomes compared with placebo in patients with IPF receiving standard of care treatment with pirfenidone or nintedanib or in those not receiving standard of care treatment. Trial Registration ClinicalTrials.gov Identifiers: NCT03711162 and NCT03733444
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- 2023
12. An Application of Queueing Theory on the Ticketing Booth of Light Rail Transit 1 (LRT-1) Central Station
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Reyes, Eunice Gabrielle A., primary, Cangco, Gerome Kaye M., additional, Ilagan, Shiela Mae C., additional, Pacunayen, Hazel P., additional, Piamonte, Jeunise A., additional, and German, Josephine D., additional
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- 2023
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13. Early therapy in Fabry disease: Outcomes from the Canadian Fabry Disease Initiative (CFDI) registry
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West, Michael L., primary, Bichet, Daniel G., additional, Iwanochko, Mark, additional, Khan, Aneal, additional, Sirrs, Sandra, additional, and LeMoine, Kaye M., additional
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- 2023
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14. A Comparison on the Usability of Mobile e-Wallet Applications: GCash, Maya, Grabpay
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Gumasing, Ma. Janice J., primary, Cangco, Gerome Kaye M, additional, Ilagan, Shiela Mae C, additional, and Reyes, Eunice Gabrielle A., additional
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- 2023
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15. What is 'good' domestic violence lawyering? Views from specialist legal services in Australia
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Wangmann, J, Bartlett, F, Batagol, B, Booth, T, Douglas, H, Kaye, M, Seear, K, Wangmann, J, Bartlett, F, Batagol, B, Booth, T, Douglas, H, Kaye, M, and Seear, K
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There is increasing recognition that many lawyers, regardless of their practice area, will represent people who have both used and experienced violence in their intimate relationships. This suggests that being informed about domestic and family violence (DFV) is an important attribute of competent lawyering. Despite repeated reports and research drawing attention to poor practices, many lawyers lack DFV training. There is limited research about what it means to be a ‘good’ DFV lawyer. To explore this, we conducted focus groups with lawyers employed in specialist women’s legal services in Australia. Much work undertaken in these services involves working with clients who have experienced DFV, so these lawyers are well placed to identify features of ‘good’ DFV lawyering. The attributes for competent lawyering with people who have experienced DFV, include: being knowledgeable about DFV, being trauma-informed/responsive, being knowledgeable about the law and the relevance of violence, adopting a safety lens and providing a holistic service. Our study identifies a number of areas that require further research including understanding the difference between DFV-informed lawyering and trauma-informed/responsive lawyering, and whether specific competencies are required when representing people who experience DFV or have used DFV. Despite years of research and various inquiries pointing to inadequacies and making recommendations for improvement, this article draws attention to continuing gaps in this area.
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- 2023
16. Redressing the balance: how Australia’s approach under the Hague Abduction Convention is still endangering victims of domestic violence
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Gray, A, Kaye, M, Gray, A, and Kaye, M
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This article explores how Australian courts consider allegations of domestic violence under Article 13(1)(b) of the Hague Convention on Child Abduction. As is now well known, there has been a paradigm shift since the Convention's introduction, which has seen the circumstances and underlying reasons for international child abduction change. Through an examination of Australian case law from the past 5 years, this article reveals the issues that taking mothers continue to face in Australian return proceedings and the concerning reality that the courts continue to prioritise a prompt return over the safety and well-being of women and children exposed to domestic violence. This is despite feminist legal scholars and activists demonstrating for over 20 years that domestic violence is not being adequately dealt with in return proceedings and calling for change. The article discusses how the huge inequality in legal representation for taking mothers and left-behind fathers adds to the imbalance in these matters. Unfortunately, the recent Hague Conference Guide to Good Practice in such cases is unlikely to affect any substantive change to redress that imbalance. Suggestions for ways to redress the balance between taking mothers and left-behind fathers are suggested.
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- 2023
17. Prenuptial agreements — What’s happening?
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Kaye, M, Sarmas, L, Fehlberg, B, Smyth, B, Kaye, M, Sarmas, L, Fehlberg, B, and Smyth, B
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Pre or early relationship (‘prenup’) financial agreements have been available in Australia for some years now, but there is still a dearth of research regarding how they are being used by lawyers and their clients. This article draws on interviews with family lawyers regarding prenup agreements, with a focus on who is using them and why, and what lawyers think about them. Key findings include that while most participants considered that power imbalance usually or often existed between the parties and was not overcome by the process of entering agreements, most also supported the continued availability of Family Law Act financial agreements — a position that, in our view, indicates the need for further legislative reform.
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- 2023
18. Synthesis of Bioethanol from Pineapple Fruit Rejects: Prospect for Fuel Blends in Spark-Ignition Engines
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Lonamie D. Carreon, Angel Kaye M. Arazo, John Michael A. Valdon, Johnah Marie D Vieña, Maribel S. Tizo, Alexander L. Ido, and Renato O. Arazo
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Chemistry (miscellaneous) ,Environmental Chemistry ,Physical and Theoretical Chemistry ,Catalysis - Published
- 2023
19. Microplastics in surface water of Laguna de Bay: first documented evidence on the largest lake in the Philippines
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Arcadio, Cris Gel Loui A., primary, Navarro, Carl Kenneth P., additional, Similatan, Kaye M., additional, Inocente, Sherley Ann T., additional, Ancla, Sheila Mae B., additional, Banda, Marybeth Hope T., additional, Capangpangan, Rey Y., additional, Torres, Armi G., additional, and Bacosa, Hernando P., additional
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- 2022
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20. Unraveling Microplastic Pollution in Mangrove Sediments of Butuan Bay, Philippines
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Navarro, Carl Kenneth P., primary, Arcadio, Cris Gel Loui A., additional, Similatan, Kaye M., additional, Inocente, Sherley Ann T., additional, Banda, Marybeth Hope T., additional, Capangpangan, Rey Y., additional, Torres, Armi G., additional, and Bacosa, Hernando P., additional
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- 2022
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21. Unraveling Microplastic Pollution in Mangrove Sediments of Butuan Bay, Philippines
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Carl Kenneth P. Navarro, Cris Gel Loui A. Arcadio, Kaye M. Similatan, Sherley Ann T. Inocente, Marybeth Hope T. Banda, Rey Y. Capangpangan, Armi G. Torres, and Hernando P. Bacosa
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Renewable Energy, Sustainability and the Environment ,Geography, Planning and Development ,Building and Construction ,Management, Monitoring, Policy and Law ,microplastics ,mangroves ,sediment ,ATR–FTIR ,Mindanao ,Philippines - Abstract
The mass production and consumption of plastics have serious effects on the environment, human health, and livelihood. Hence, global efforts to reduce plastic generation must be realized. This study aimed to determine the prevalence of microplastics in mangrove sediments of Cabadbaran, Buenavista, and Nasipit in Butuan Bay, Philippines. Seventy-two (72) microplastic particles were extracted from mangrove sediments dominated by fibrous type (71%) and blue (35%) as the most common color. Attenuated total reflectance–Fourier transform infrared (ATR–FTIR) spectroscopy was used to assess the polymer type of microplastics. Results reveal a total of six polymer types including high-density polyethylene, low-density polyethylene, polyethylene terephthalate, ethylene-vinyl acetate, polyamide, and polypropylene, with the latter comprising 39% of samples, the highest among the extracted particles. Overall, Nasipit (71.1/kg) obtained the highest microplastic density followed by Buenavista (48.9/kg) and Cabadbaran (40.0/kg). These data will serve as a piece of baseline information in crafting important environmental policies to address plastic pollution issues in the area. Long-term studies are recommended to better understand, monitor, and prevent further microplastic pollution in Butuan Bay.
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- 2022
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22. Microplastics in surface water of Laguna de Bay: First documented evidence on the largest lake in the Philippines
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Cris Gel Loui A. Arcadio, Carl Kenneth P. Navarro, Kaye M. Similatan, Sherley Ann T. Inocente, Sheila Mae B. Ancla, Marybeth Hope T. Banda, Rey Y. Capangpangan, Armi G. Torres, and Hernando P. Bacosa
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Health, Toxicology and Mutagenesis ,Environmental Chemistry ,General Medicine ,Pollution - Abstract
The pollution of aquatic systems by microplastics is a well-known environmental problem. However, limited studies have been conducted in freshwater systems, especially in the Philippines. Here we determined for the first time the amount of microplastics in the Philippines’ largest freshwater lake, the Laguna de Bay. Ten (10) sampling stations on the lake's surface water were sampled using a plankton net. Samples were extracted and analyzed using Fourier transformed infrared spectroscopy (FTIR). A total of 100 microplastics were identified from 10 sites with a mean density of 14.29 items/m3. The majority of microplastics were fibers (57%), while blue-colored microplastics predominated in the sampling areas (53%). There were 11 microplastic polymers identified predominantly polypropylene (PP), ethylene vinyl acetate copolymer (EVA), and polyethylene terephthalate (PET), which together account for 65% of the total microplastics in the areas. The results show that there is a higher microplastic density in areas with high relative population density which necessitates the implementation of proper plastic waste management measures in the communities operating on the lake and in its vicinity to protect the lake's ecosystem services. Furthermore, future research should also focus on the environmental risks posed by these microplastics, especially on the fisheries and aquatic resources.
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- 2022
23. Quitting is such a sweet sorrow: a grounded theory study of unassisted smoking cessation among Filipino adult smokers
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Domingo, Jochelle Sylvia F., primary, Reyes, Kirtana Dasi T., additional, Villegas, Cathrine Kaye M., additional, Icarangal, Allison Joseph S., additional, and Valencia-Raymundo, Agnes, additional
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- 2022
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24. Early therapy in Fabry disease: Outcomes from the Canadian Fabry Disease Initiative (CFDI) registry
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Michael L. West, Daniel G. Bichet, Mark Iwanochko, Aneal Khan, Sandra Sirrs, and Kaye M. LeMoine
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Endocrinology ,Endocrinology, Diabetes and Metabolism ,Genetics ,Molecular Biology ,Biochemistry - Published
- 2023
25. Addressing the problem of direct cross-examination in Australian family law proceedings
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Wangmann, J, Kaye, M, Booth, T, Wangmann, J, Kaye, M, and Booth, T
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Difficulties experienced by victims of family violence who are cross-examined by the unrepresented perpetrator of that violence (or vice versa) in family law proceedings are well-documented. Such direct cross-examination can be traumatic and unlikely to generate high quality evidence. In 2019 this problem was addressed in Australia by the Family Violence and Cross-Examination Scheme (‘Scheme’). Under this Scheme, direct cross-examination by self-represented litigants is prohibited on a mandatory or discretionary basis in certain family law cases involving allegations of family violence. This article examines the implementation of the Scheme by drawing on data from a large ethnographic project that was concerned with self-representation in family law proceedings involving allegations of family violence and an analysis of recent case law. We highlight issues in the early administration of the Scheme as well as more complex ongoing issues. This article provides an evidence base to guide policy and legislative developments in this area.
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- 2022
26. The university teaching of family law
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Kaye, M, Jones, J, Kaye, M, and Jones, J
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We have co-taught a family law property subject over the last 2 years. The experience has led us to reflect upon the content, structure and teaching methodology of family law courses. Reflecting upon our teaching has cemented our views that some knowledge of family law should be core knowledge for all law graduates. In particular, we believe that competencies required for good family law practice (understanding family violence; child-abuse; trauma-informed practice; and cultural awareness) are essential requirements for all legal graduates. The remainder of the article is a conversation outlining our thoughts and experiences in relation to the teaching of Family law which we hope will contribute to and inform broader debates about the role of the legal academy, legal education, and the place of vocational qualifications within them. Such a conversation is particularly important in the age of the neoliberal university and the recasting of law as a purely vocational skill. It is hoped that the article starts a conversation about the future purpose and meaning of the university study of family law.
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- 2022
27. Family violence and family law property division: How can the system be improved?
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Kaye, M and Kaye, M
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This article explores how the economic impacts of violence can continue long after a relationship has ended and how the family law system is a generally unhelpful tool for financial recovery from abuse. Hurdles in the legal system such as the unavailability of legal aid and the absence of affordable legal representation, lack of legislative reforms in relation to property division in cases of violence, and difficulties in enforcing orders, compound experiences of violence and entrench poverty. Reforms to improve the family law property system for victims of violence are suggested.
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- 2022
28. From Surface Water to the Deep Sea: A Review on Factors Affecting the Biodegradation of Spilled Oil in Marine Environment
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Bacosa, Hernando Pactao, primary, Ancla, Sheila Mae B., additional, Arcadio, Cris Gel Loui A., additional, Dalogdog, John Russel A., additional, Ellos, Dioniela Mae C., additional, Hayag, Heather Dale A., additional, Jarabe, Jiza Gay P., additional, Karim, Ahl Jimhar T., additional, Navarro, Carl Kenneth P., additional, Palma, Mae Princess I., additional, Romarate, Rodolfo A., additional, Similatan, Kaye M., additional, Tangkion, Jude Albert B., additional, Yurong, Shann Neil A., additional, Mabuhay-Omar, Jhonamie A., additional, Inoue, Chihiro, additional, and Adhikari, Puspa L., additional
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- 2022
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29. Effects of Coffee Consumption on the Psychological Wellbeing of Undergraduate Students during Online Learning.
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Bajar, Raven Gio Charles A., Cangco, Gerome Kaye M., Dantis, Jimmuel R., Marcos, Stephen Shadrach P., and Gumasing, Mariya Janice J.
- Subjects
COFFEE ,ONLINE education ,UNDERGRADUATES ,PSYCHOLOGICAL well-being ,MENTAL health - Abstract
This study evaluates undergraduate students' coffee consumption levels and investigates how it affects their psychological well-being during online learning. Caffeine is a psychostimulant that boosts body energy levels, and coffee is its primary medium in beverages, so it is popular among students due to their need for help studying, especially during online classes. However, caffeine also has adverse effects. The researchers gathered data from a sample of 100 college students through a digital survey that aimed to know their level of coffee consumption and general mental health status using the GHQ-12 questionnaire. The GHQ-12 generates social dysfunction, anxiety and depression, and loss of confidence. The data obtained from the respondents were analyzed using descriptive and inferential statistics, and measurements of this study have determined that age and social dysfunction are associated with coffee consumption. It was concluded that age is directly proportional to coffee consumption, and students with high social dysfunction tend to consume a high amount of coffee. The researchers recommend that educational institutions must hold seminars for their students to discuss this awareness and encourage dosage limits of coffee to students. [ABSTRACT FROM AUTHOR]
- Published
- 2022
30. Quitting is such a sweet sorrow: a grounded theory study of unassisted smoking cessation among Filipino adult smokers
- Author
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Jochelle Sylvia F. Domingo, Kirtana Dasi T. Reyes, Cathrine Kaye M. Villegas, Allison Joseph S. Icarangal, and Agnes Valencia-Raymundo
- Subjects
Public Health, Environmental and Occupational Health ,Emergency Medicine - Published
- 2022
31. Mental Health in Medicine: A novel stepped care model in medical psychiatry and the implementation of measurement-based care.
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Wiercigroch, D., Wright, S., Bangloy, N., Bush, S., Wai, A., Koshy, D., Thomson, L., Abdel Gawad, N., Abbey, S., Kaye, M., Kelsey, M., Tan, A., Delwo, J., and Sheehan, K.
- Subjects
OUTPATIENT medical care ,SOCIAL workers ,PATIENT reported outcome measures ,TERTIARY care ,ERYTHROCYTES ,CONSULTATION-liaison psychiatry - Abstract
Introduction: Individuals with co-occurring mental and physical health issues have worse health outcomes in both domains. Integration improves outcomes and aligns with patient preference, but health services tend to be siloed. The Mental Health in Medicine Clinic (MHiM) supports patients receiving inpatient or outpatient medical or surgical care at a tertiary academic hospital in Toronto, Canada. The predominantly virtual clinic has an interdisciplinary team offering services via stepped care, matching patient need with service intensity. Measurement-based care (MBC), the systematic evaluation of patient reported outcomes, was not initially used routinely in the clinic, but its implementation may improve treatment decision-making and may be useful in allocating patients within a stepped care model. Objectives: 1) To describe the stepped care model, referral patterns, diagnoses, and level of care provided since implementation of stepped care. 2) To conduct a quality improvement initiative to implement MBC in the clinic, with a goal of 50% of patients completing at the time of first assessment and prior to discharge from the clinic. Methods: We reviewed the electronic medical record for referral source, diagnoses, and level of stepped care within the clinic. We conducted semi-structured interviews with stakeholders (clinicians, administrative staff, patients) to explore barriers to implementation of MBC. Interviews were analyzed for themes around barriers and facilitators to MBC. Plan, Do, Study, Act cycles were carried out around change concepts informed by stakeholder interviews and relevant literature. Results: The MHiM clinic began operations in August 2020. The clinic operated on a physician-only model until March 2022 and then shifted to a stepped care model with an interdisciplinary team. The most frequent referral sources were internal medicine, COVID19 clinics, consultation-liaison psychiatry, red blood cell disorders clinic and cardiology. Since the implementation of stepped care, 250 referrals were assessed. 58% of new referrals were assessed by the psychiatrist, 42% were managed by the NP, and 25% consulted with the social worker. Referrals consisted of trauma and stress-related disorders (32%), depression (21%) or anxiety disorders (20%). Personality, substance use, and psychotic disorders accounted for less than 10% of referrals combined. Some patients did not have any diagnosis (6%). Results from the quality improvement initiative to implement MBC will also be presented. Conclusions: The MHiM clinic provides an integrated care pathway addressing comorbid mental and physical health conditions. We describe a novel stepped care model and the implementation of MBC. Future directions include ongoing quality improvement of MBC and its integration within the clinic to assess and re-assess service intensity. Disclosure of Interest: None Declared [ABSTRACT FROM AUTHOR]
- Published
- 2024
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32. Factorial validity and gender invariance of the Patient Health Questionnaire (PHQ-9) in student-athletes.
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Harenberg S, Keenan L, Ingram Y, Wilson S, Vosloo J, and Kaye M
- Abstract
Background/purpose: Depressive symptoms are prevalent in student-athletes. Evidence for the factorial validity of measures assessing depressive symptoms in student-athletes is presently absent from the literature. This study examined the best fitting factorial structure and invariance across sexes of the PHQ-9., Methods: Data were collected from 1,524 student-athletes from institutions in the North-Eastern United States. Confirmatory factor analyses of 4 factorial structures of the Patient Health Questionnaire-9 (PHQ-9) were conducted, followed by invariance analyses across sexes., Results: All tested models showed an excellent fit for all tested models and a unidimensional model of the PHQ-9 was chosen for further analyses. Metric and scalar invariance for the identified model across sexes was supported. The participants in this study reported an unusually low (3.13%) prevalence of elevated depressive symptoms, which may indicate underreporting of symptoms., Conclusion: Using a unidimensional, composite score via the PHQ-9 is a valid assessment of depressive symptoms in male and female student-athletes.
- Published
- 2024
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33. PROTAC-Mediated Dual Degradation of BCL-xL and BCL-2 Is a Highly Effective Therapeutic Strategy in Small-Cell Lung Cancer.
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Khan S, Cao L, Wiegand J, Zhang P, Zajac-Kaye M, Kaye FJ, Zheng G, and Zhou D
- Subjects
- Humans, Mice, Animals, bcl-X Protein metabolism, Proto-Oncogene Proteins c-bcl-2 metabolism, Disease Models, Animal, Lung Neoplasms drug therapy, Small Cell Lung Carcinoma pathology, Antineoplastic Agents pharmacology, Thrombocytopenia, Aniline Compounds, Sulfonamides, Bridged Bicyclo Compounds, Heterocyclic
- Abstract
BCL-xL and BCL-2 are validated therapeutic targets in small-cell lung cancer (SCLC). Targeting these proteins with navitoclax (formerly ABT263, a dual BCL-xL/2 inhibitor) induces dose-limiting thrombocytopenia through on-target BCL-xL inhibition in platelets. Therefore, platelet toxicity poses a barrier in advancing the clinical translation of navitoclax. We have developed a strategy to selectively target BCL-xL in tumors, while sparing platelets, by utilizing proteolysis-targeting chimeras (PROTACs) that hijack the cellular ubiquitin proteasome system for target ubiquitination and subsequent degradation. In our previous study, the first-in-class BCL-xL PROTAC, called DT2216, was shown to have synergistic antitumor activities when combined with venetoclax (formerly ABT199, BCL-2-selective inhibitor) in a BCL-xL/2 co-dependent SCLC cell line, NCI-H146 (hereafter referred to as H146), in vitro and in a xenograft model. Guided by these findings, we evaluated our newly developed BCL-xL/2 dual degrader, called 753b, in three BCL-xL/2 co-dependent SCLC cell lines and the H146 xenograft models. 753b was found to degrade both BCL-xL and BCL-2 in these cell lines. Importantly, it was considerably more potent than DT2216, navitoclax, or DT2216 + venetoclax in reducing the viability of BCL-xL/2 co-dependent SCLC cell lines in cell culture. In vivo, 5 mg/kg weekly dosing of 753b was found to lead to significant tumor growth delay, similar to the DT2216 + venetoclax combination in H146 xenografts, by degrading both BCL-xL and BCL-2. Additionally, 753b administration at 5 mg/kg every four days induced tumor regressions. At this dosage, 753b was well tolerated in mice, without observable induction of severe thrombocytopenia as seen with navitoclax, and no evidence of significant changes in mouse body weights. These results suggest that the BCL-xL/2 dual degrader could be an effective and safe therapeutic for a subset of SCLC patients, warranting clinical trials in future.
- Published
- 2024
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34. Key learnings from the INBUILD trial in patients with progressive pulmonary fibrosis.
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Mira-Avendano I and Kaye M
- Subjects
- Humans, Vital Capacity, Severity of Illness Index, Treatment Outcome, Randomized Controlled Trials as Topic, Glucocorticoids administration & dosage, Glucocorticoids adverse effects, Glucocorticoids therapeutic use, Lung drug effects, Lung physiopathology, Lung diagnostic imaging, Lung Diseases, Interstitial drug therapy, Lung Diseases, Interstitial physiopathology, Lung Diseases, Interstitial diagnosis, Idiopathic Pulmonary Fibrosis drug therapy, Idiopathic Pulmonary Fibrosis physiopathology, Idiopathic Pulmonary Fibrosis diagnosis, Male, Protein Kinase Inhibitors adverse effects, Protein Kinase Inhibitors therapeutic use, Protein Kinase Inhibitors administration & dosage, Indoles adverse effects, Indoles therapeutic use, Indoles administration & dosage, Disease Progression, Pulmonary Fibrosis drug therapy, Pulmonary Fibrosis physiopathology, Tomography, X-Ray Computed
- Abstract
In a patient with interstitial lung disease (ILD) of known or unknown etiology other than idiopathic pulmonary fibrosis (IPF), progressive pulmonary fibrosis (PPF) is defined by worsening lung fibrosis on high-resolution computed tomography (HRCT), decline in lung function, and/or deterioration in symptoms. The INBUILD trial involved 663 patients with PPF who were randomized to receive nintedanib or placebo. The median exposure to trial medication was approximately 19 months. The INBUILD trial provided valuable learnings about the course of PPF and the efficacy and safety of nintedanib. The relative effect of nintedanib on reducing the rate of forced vital capacity decline was consistent across subgroups based on ILD diagnosis, HRCT pattern, and disease severity at baseline, and between patients who were and were not taking glucocorticoids or disease-modifying anti-rheumatic drugs and/or glucocorticoids at baseline. The adverse events most frequently associated with nintedanib were gastrointestinal, particularly diarrhea, but nintedanib was discontinued in only a minority of cases. The results of the INBUILD trial highlight the importance of prompt detection and treatment of PPF and the utility of nintedanib as a treatment option.
- Published
- 2024
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35. Corrigendum: Targeting thymidylate synthase enhances the chemosensitivity of triple-negative breast cancer towards 5-FU-based combinatorial therapy.
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Haritha NH, Nawab A, Vijayakurup V, Anto NP, Liju VB, Alex VV, Amrutha AN, Aiswarya SU, Swetha M, Vinod BS, Sundaram S, Guijarro MV, Herlevich T, Krishna A, Nestory NK, Bava SV, Sadasivan C, Zajac-Kaye M, and Anto RJ
- Abstract
[This corrects the article DOI: 10.3389/fonc.2021.656804.]., (Copyright © 2023 Haritha, Nawab, Vijayakurup, Anto, Liju, Alex, Amrutha, Aiswarya, Swetha, Vinod, Sundaram, Guijarro, Herlevich, Krishna, Nestory, Bava, Sadasivan, Zajac-Kaye and Anto.)
- Published
- 2023
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36. Australian National Enterovirus Reference Laboratory annual report, 2022.
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Kaye M, Hobday L, Ibrahim A, Bruggink L, and Thorley B
- Subjects
- Child, Humans, alpha-Fetoproteins, Australia epidemiology, Disease Notification, Feces, Enterovirus, Enterovirus Infections epidemiology, Poliomyelitis epidemiology, Poliomyelitis prevention & control
- Abstract
Abstract: Australia monitors its polio-free status by conducting surveillance for cases of acute flaccid paralysis (AFP) in children less than 15 years of age, as recommended by the World Health Organization (WHO). Cases of AFP in children are notified to the Australian Paediatric Surveillance Unit or the Paediatric Active Enhanced Disease Surveillance System, and faecal specimens are referred for virological investigation to the National Enterovirus Reference Laboratory. In 2022, no cases of poliomyelitis were reported from clinical surveillance and Australia reported 1.69 non-polio AFP cases per 100,000 children, thereby meeting the WHO's performance criterion for a sensitive surveillance system. The non-polio enteroviruses coxsackievirus A2, coxsackievirus A6, coxsackievirus A10, echovirus 18, enterovirus A71 and enterovirus C96 were identified from clinical specimens collected from AFP cases. Australia also performs enterovirus and environmental surveillance to complement the clinical system focussed on children. In 2022, thirty cases of wild poliovirus were reported from three countries (Afghanistan, Mozambique and Pakistan); 24 countries also reported cases of poliomyelitis due to circulating vaccine-derived poliovirus., (© Commonwealth of Australia CC BY-NC-ND.)
- Published
- 2023
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37. TYMS promotes genomic instability and tumor progression in Ink4a/Arf null background.
- Author
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Guijarro MV, Nawab A, Dib P, Burkett S, Luo X, Feely M, Nasri E, Seifert RP, Kaye FJ, and Zajac-Kaye M
- Subjects
- Animals, Humans, Mice, Cell Transformation, Neoplastic genetics, Cyclin-Dependent Kinase Inhibitor p16 genetics, Cyclin-Dependent Kinase Inhibitor p16 metabolism, Genomic Instability, Tumor Suppressor Protein p14ARF, Neoplasms genetics, Thymidylate Synthase genetics
- Abstract
We previously showed that elevated TYMS exhibits oncogenic properties and promotes tumorigenesis after a long latency, suggesting cooperation with sequential somatic mutations. Here we report the cooperation of ectopic expression of human TYMS with loss of Ink4a/Arf, one of the most commonly mutated somatic events in human cancer. Using an hTS/Ink4a/Arf
-/- genetically engineered mouse model we showed that deregulated TYMS expression in Ink4a/Arf null background accelerates tumorigenesis and metastasis. In addition, tumors from TYMS-expressing mice were associated with a phenotype of genomic instability including enhanced double strand DNA damage, aneuploidy and loss of G1/S checkpoint. Downregulation of TYMS in vitro decreased cell proliferation and sensitized tumor cells to antimetabolite chemotherapy. In addition, depletion of TYMS in vivo by TYMS shRNA reduced tumor incidence, delayed tumor progression and prolonged survival in hTS/Ink4a/Arf-/- mice. Our data shows that activation of TYMS in Ink4a/Arf null background enhances uncontrolled cell proliferation and tumor growth, supporting the development of new agents and strategies targeting TYMS to delay tumorigenesis and prolong survival., (© 2023. The Author(s).)- Published
- 2023
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38. First-in-class multifunctional TYMS nonclassical antifolate inhibitor with potent in vivo activity that prolongs survival.
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Guijarro MV, Kellish PC, Dib PE, Paciaroni NG, Nawab A, Andring J, Kulemina L, Borrero NV, Modenutti C, Feely M, Nasri E, Seifert RP, Luo X, Bennett RL, Shabashvili D, Licht JD, McKenna R, Roitberg A, Huigens RW 3rd, Kaye FJ, and Zajac-Kaye M
- Subjects
- Mice, Animals, Humans, Enzyme Inhibitors pharmacology, Drug Resistance, Thymidylate Synthase, Folic Acid Antagonists pharmacology, Folic Acid Antagonists therapeutic use, Folic Acid Antagonists chemistry
- Abstract
Although thymidylate synthase (TYMS) inhibitors have served as components of chemotherapy regimens, the currently available inhibitors induce TYMS overexpression or alter folate transport/metabolism feedback pathways that tumor cells exploit for drug resistance, limiting overall benefit. Here we report a small molecule TYMS inhibitor that i) exhibited enhanced antitumor activity as compared with current fluoropyrimidines and antifolates without inducing TYMS overexpression, ii) is structurally distinct from classical antifolates, iii) extended survival in both pancreatic xenograft tumor models and an hTS/Ink4a/Arf null genetically engineered mouse tumor model, and iv) is well tolerated with equal efficacy using either intraperitoneal or oral administration. Mechanistically, we verify the compound is a multifunctional nonclassical antifolate, and using a series of analogs, we identify structural features allowing direct TYMS inhibition while maintaining the ability to inhibit dihydrofolate reductase. Collectively, this work identifies nonclassical antifolate inhibitors that optimize inhibition of thymidylate biosynthesis with a favorable safety profile, highlighting the potential for enhanced cancer therapy.
- Published
- 2023
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39. Co-targeting BCL-X L and MCL-1 with DT2216 and AZD8055 synergistically inhibit small-cell lung cancer growth without causing on-target toxicities in mice.
- Author
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Khan S, Kellish P, Connis N, Thummuri D, Wiegand J, Zhang P, Zhang X, Budamagunta V, Hua N, Yang Y, De U, Jin L, Zhang W, Zheng G, Hromas R, Hann C, Zajac-Kaye M, Kaye FJ, and Zhou D
- Abstract
Small-cell lung cancer (SCLC) is an aggressive malignancy with limited therapeutic options. The dismal prognosis in SCLC is in part associated with an upregulation of BCL-2 family anti-apoptotic proteins, including BCL-X
L and MCL-1. Unfortunately, the currently available inhibitors of BCL-2 family anti-apoptotic proteins, except BCL-2 inhibitors, are not clinically relevant because of various on-target toxicities. We, therefore, aimed to develop an effective and safe strategy targeting these anti-apoptotic proteins with DT2216 (our platelet-sparing BCL-XL degrader) and AZD8055 (an mTOR inhibitor) to avoid associated on-target toxicities while synergistically optimizing tumor response. Through BH3 mimetic screening, we identified a subset of SCLC cell lines that is co-dependent on BCL-XL and MCL-1. After screening inhibitors of selected tumorigenic pathways, we found that AZD8055 selectively downregulates MCL-1 in SCLC cells and its combination with DT2216 synergistically killed BCL-XL /MCL-1 co-dependent SCLC cells, but not normal cells. Mechanistically, the combination caused BCL-XL degradation and suppression of MCL-1 expression, and thus disrupted MCL-1 interaction with BIM leading to an enhanced apoptotic induction. In vivo, the DT2216 + AZD8055 combination significantly inhibited the growth of cell line-derived and patient-derived xenografts and reduced tumor burden accompanied by increased survival in a genetically engineered mouse model of SCLC without causing appreciable thrombocytopenia or other normal tissue injuries. Thus, these preclinical findings lay a strong foundation for future clinical studies to test DT2216 + mTOR inhibitor combinations in a subset of SCLC patients whose tumors are co-driven by BCL-XL and MCL-1., (© 2023. The Author(s).)- Published
- 2023
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40. Thymidylate synthase accelerates Men1-mediated pancreatic tumor progression and reduces survival.
- Author
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Vijayakurup V, Maeng K, Lee HS, Meyer B, Burkett S, Nawab A, Dougherty MW, Jobin C, Mahmud I, Garrett TJ, Feely M, Lee KB, Kaye FJ, Guijarro MV, and Zajac-Kaye M
- Subjects
- Animals, Humans, Mice, Pancreas pathology, Proto-Oncogene Proteins genetics, Thymidylate Synthase genetics, Neuroendocrine Tumors genetics, Neuroendocrine Tumors metabolism, Pancreatic Neoplasms pathology
- Abstract
Clinical studies of cancer patients have shown that overexpression or amplification of thymidylate synthase (TS) correlates with a worse clinical outcome. We previously showed that elevated TS exhibits properties of an oncogene and promotes pancreatic neuroendocrine tumors (PanNETs) with a long latency. To study the causal impact of elevated TS levels in PanNETs, we generated a mouse model with elevated human TS (hTS) and conditional inactivation of the Men1 gene in pancreatic islet cells (hTS/Men1-/-). We demonstrated that increased hTS expression was associated with earlier tumor onset and accelerated PanNET development in comparison with control Men1-/- and Men1+/ΔN3-8 mice. We also observed a decrease in overall survival of hTS/Men1+/- and hTS/Men1-/- mice as compared with control mice. We showed that elevated hTS in Men1-deleted tumor cells enhanced cell proliferation, deregulated cell cycle kinetics, and was associated with a higher frequency of somatic mutations, DNA damage, and genomic instability. In addition, we analyzed the survival of 88 patients with PanNETs and observed that high TS protein expression independently predicted worse clinical outcomes. In summary, elevated hTS directly participates in promoting PanNET tumorigenesis with reduced survival in Men1-mutant background. This work will refocus attention on new strategies to inhibit TS activity for PanNET treatment.
- Published
- 2022
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41. Microbial community profiling by next-generation DNA sequencing of adenocarcinoma of the prostate with evidence of ochratoxin A producing fungi.
- Author
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Fry SE, Kaye M, Missan DS, Becker C, Shabilla M, Martinez D, Bossert E, and Ellis J
- Subjects
- Humans, Male, Middle Aged, Prostate, Fungi genetics, DNA, Ribosomal genetics, Sequence Analysis, DNA, Microbiota genetics, Adenocarcinoma genetics
- Abstract
Background: Prostatic carcinomas are a leading cancer and leading cause of mortality in the developed world. The etiology is diverse with underlying patient genetics, environmental factors, and microbial associations. Sequencing DNA for microbes allows the detection of potential disease relationships., Objective: Targeted 16S (prokaryotic) and 18S (eukaryotic) rDNA sequencing was performed to map the tumor microbial flora., Design: Twelve patients undergoing elective laparoscopic prostatectomy for biopsy proven adenocarcinoma of the prostate were enrolled. PCR and amplicon based sequencing was conducted; a portion of the sequencing results were confirmed by special stains., Setting: Patients were recruited by the urologist were prospectively scheduled for radical prostatectomy by 'Da Vinci' robotically assisted procedure in an outpatient setting. Samples were portioned in the hospital surgical suite at the time of prostatectomy., Participants: Male patients were requested to enter the study on a first come basis., Outcome Measurement and Statistical Analysis: Average age of the 12 participants was 64.3 years., Results and Limitations: DNA reads were detected and by 'best match' were identified belonging to Perkinsus, Hydrurus, Diversispora and Funneliformis genera, few samples displayed bacteria. Out of the 12 total patients, 11 patients had detectable DNA sequences matching arbuscular mycorrhizal fungi in the Glomeromycetes Class; Funneliformis mosseae and Diversasporum versiformis. Specific PCR for arbuscular mycorrhizal fungi failed to confirm Glomeromycetes Class; in-depth taxonomic analysis suggests a newer fungal grouping, not falling within an accepted Phylum of fungi. Calcoflour white staining of histological sections confirmed potential fungal markers in all 12 cases. Ochratoxin A antigen was identified by immunofluorescence in all 12 patient samples. The study was limited by the low sample volume and disease free normal controls., Conclusions: Fungi may play a significant role in adenocarcinoma of the prostate., Competing Interests: Declaration of Competing Interest Stephen E. Fry – owner of Fry Laboratories, BioID Genomics, and founder of The Southwest Center for Chronic Disease. All other authors have no conflicts: We acknowledge the Southwest Center for Chronic Disease for their support., (Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2022
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42. Concomitant Pulmonary Embolism and Anterior Myocardial Infarction as the Initial Presentation of Antiphospholipid Syndrome.
- Author
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Bahuva R, Khan HA, Amuthan R, Arrieta JC, Farooq W, Mir A, Kaye M, and Phadke K
- Abstract
Competing Interests: The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
- Published
- 2022
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43. A case series to examine the perinatal outcomes of infants conceived by intravaginal culture (IVC).
- Author
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Kaye M, Williams E, Anderson A, Arredondo F, Pike J, and Mak W
- Subjects
- Birth Weight, Female, Fertilization in Vitro, Humans, Infant, Newborn, Infant, Premature, Population Surveillance, Pregnancy, Pregnancy Outcome, Pregnancy, Twin, Reproductive Techniques, Assisted, Retrospective Studies, Infertility, Premature Birth
- Abstract
Purpose: In vitro fertilization (IVF) has been a well-established method for treating infertility for over four decades. The mainstay method of culture of oocytes and embryos has been in gas incubators. More recently, the novel use of a gas-permeable closed vessel to culture oocytes and embryos in the vagina, intravaginal culture (IVC), has been introduced as a viable lower-cost option for infertility patients. Several studies have studied the efficacy of IVC; however, there is no data on the perinatal outcomes of the babies born using this newer technology., Methods: Our study is a retrospective case series (n = 66) from a single center, uniquely examining the perinatal outcomes of infants born after IVC., Results: There were 50 singleton and 16 twin gestations in this case series. For singleton infants conceived via IVC (n = 50), the mean gestational age at delivery was 38 weeks and 4 days, and the mean birth weight was 3159.1 + / - 501.5 g. Four infants were born with low birth weight, three were born preterm, and one was born macrosomic. The twin pregnancies had a mean gestational age at delivery of 33 weeks 4 days and a mean birth weight of 1992.9 + / - 620.7 g. Twenty-seven infants met the criteria for low birthweight, and twenty-four infants delivered preterm. No twin infants met the criteria for macrosomia., Conclusion: This case series provides an initial description of the perinatal outcomes of IVC conceived infants, which shows no concerning trends in adverse birth outcomes for singleton infants. As expected, IVC twin gestations had a high rate of low birth weight and preterm delivery. Continued larger studies are essential to provide more comprehensive data on perinatal outcomes of infants conceived by this new technology., (© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
- Published
- 2022
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44. BCL-X L PROTAC degrader DT2216 synergizes with sotorasib in preclinical models of KRAS G12C -mutated cancers.
- Author
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Khan S, Wiegand J, Zhang P, Hu W, Thummuri D, Budamagunta V, Hua N, Jin L, Allegra CJ, Kopetz SE, Zajac-Kaye M, Kaye FJ, Zheng G, and Zhou D
- Subjects
- Humans, bcl-X Protein genetics, bcl-X Protein metabolism, Mutation, Piperazines, Proteolysis, Proto-Oncogene Proteins p21(ras) genetics, Pyridines, Pyrimidines, Carcinoma, Non-Small-Cell Lung drug therapy, Carcinoma, Non-Small-Cell Lung genetics, Lung Neoplasms drug therapy, Lung Neoplasms genetics
- Abstract
KRAS mutations are the most common oncogenic drivers. Sotorasib (AMG510), a covalent inhibitor of KRAS
G12C , was recently approved for the treatment of KRASG12C -mutated non-small cell lung cancer (NSCLC). However, the efficacy of sotorasib and other KRASG12C inhibitors is limited by intrinsic resistance in colorectal cancer (CRC) and by the rapid emergence of acquired resistance in all treated tumors. Therefore, there is an urgent need to develop novel combination therapies to overcome sotorasib resistance and to maximize its efficacy. We assessed the effect of sotorasib alone or in combination with DT2216 (a clinical-stage BCL-XL proteolysis targeting chimera [PROTAC]) on KRASG12C -mutated NSCLC, CRC and pancreatic cancer (PC) cell lines using MTS cell viability, colony formation and Annexin-V/PI apoptosis assays. Furthermore, the therapeutic efficacy of sotorasib alone and in combination with DT2216 was evaluated in vivo using different tumor xenograft models. We observed heterogeneous responses to sotorasib alone, whereas its combination with DT2216 strongly inhibited viability of KRASG12C tumor cell lines that partially responded to sotorasib treatment. Mechanistically, sotorasib treatment led to stabilization of BIM and co-treatment with DT2216 inhibited sotorasib-induced BCL-XL /BIM interaction leading to enhanced apoptosis in KRASG12C tumor cell lines. Furthermore, DT2216 co-treatment significantly improved the antitumor efficacy of sotorasib in vivo. Collectively, our findings suggest that due to cytostatic activity, the efficacy of sotorasib is limited, and therefore, its combination with a pro-apoptotic agent, i.e., DT2216, shows synergistic responses and can potentially overcome resistance., (© 2022. The Author(s).)- Published
- 2022
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45. Renal and dietary factors associated with hypertension in a setting of disadvantage in rural India.
- Author
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Evans RG, Subasinghe AK, Busingye D, Srikanth VK, Kartik K, Kalyanram K, Suresh O, Arabshahi S, Curkpatrick I, O'Dea K, Walker KZ, Kaye M, Yang J, Thomas N, Arulappan G, and Thrift AG
- Subjects
- Adult, Blood Pressure, Humans, India epidemiology, Sodium, Hypertension epidemiology, Rural Population
- Abstract
Using a case-control design, we determined risk factors associated with hypertension in a disadvantaged rural population in southern India. Three hundred adults with hypertension and 300 age- and sex-matched controls were extensively phenotyped. Underweight (29%, body mass index < 18.0 kg m
-2 ), chronic kidney disease (25%, estimated glomerular filtration rate <60 ml min-1 1.73 m-2 ) and anemia (82%) were highly prevalent. The ratio of sodium to potassium excretion was high (8.2). In multivariable conditional logistic regression of continuous variables dichotomized by their median value, hypertension was independently associated with greater abdominal adiposity as assessed by waist-hip ratio [odds ratio (95% confidence interval), 1.89 (1.21-2.97)], lesser protein intake as assessed by 24 h urea excretion [0.39 (0.24-0.65)], and lesser plasma renin activity [0.54 (0.35-0.84)]. Hypertension tended to be independently associated with lesser serum potassium concentration [0.66 (0.44-1.01), P = 0.06]. Furthermore, those with hypertension reported less frequent intake of vegetables and urinary sodium-potassium ratio correlated positively with serum sodium-potassium ratio (r = 0.18). Hypertension was also independently associated with lesser blood hemoglobin concentration [0.48 (0.26-0.88)]. Blood hemoglobin concentration was positively associated with serum iron (r = 0.41) and ferritin (r = 0.25) concentration and negatively associated with total iron binding capacity (r = -0.17), reflecting iron-deficiency anemia. Our findings indicate potential roles for deficient intake of potassium and protein, and iron-deficiency anemia, in the pathophysiology of hypertension in a setting of disadvantage in rural India. Imbalanced intake of potassium and sodium may be driven partly by deficient intake of vegetables or fruit., (© 2021. The Author(s), under exclusive licence to Springer Nature Limited part of Springer Nature.)- Published
- 2021
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46. Australian National Enterovirus Reference Laboratory annual report, 2020.
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Kaye M, Garcia-Clapes A, Hobday L, Ibrahim A, Chanthalavanh P, Bruggink L, and Thorley B
- Subjects
- Australia epidemiology, Child, Disease Notification, Feces, Humans, Laboratories, Enterovirus
- Abstract
Abstract: Australia monitors its polio-free status by conducting surveillance for cases of acute flaccid paralysis (AFP) in children less than 15 years of age, as recommended by the World Health Organization (WHO). Cases of AFP in children are notified to the Australian Paediatric Surveillance Unit or the Paediatric Active Enhanced Disease Surveillance System and faecal specimens are referred for virological investigation to the National Enterovirus Reference Laboratory. In 2020, no cases of poliomyelitis were reported from clinical surveillance; Australia reported 1.09 non-polio AFP cases per 100,000 children, thereby meeting the WHO's performance criterion for a sensitive surveillance system. The non-polio enteroviruses coxsackievirus A10 and coxsackievirus A16 were identified from clinical specimens collected from AFP cases. Australia also performs enterovirus surveillance and environmental surveillance to complement the clinical system focussed on children. In 2020, there were 140 cases of wild poliovirus reported from the two remaining endemic countries: Afghanistan and Pakistan. Another 28 countries reported cases of circulating vaccine-derived poliovirus., (© Commonwealth of Australia CC BY-NC-ND.)
- Published
- 2021
- Full Text
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