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Malignant melanoma is aggressive cancer with a high rate of local invasiveness and metastasis. Currently, the treatment options for patients with advanced-stage and metastatic oral melanoma are limited. A promising treatment option is oncolytic viral therapy. This study aimed to evaluate novel therapies for malignant melanoma using a canine model. Oral melanoma, which frequently occurs in dogs is used as a model for human melanoma, was isolated and cultured and used for the evaluation of the tumor lytic effect induced by viral infection. We constructed a recombinant Newcastle disease virus (rNDV) that promotes the extracellular release of IFNγ from the virus-infected melanoma. The expression of oncolytic and apoptosis-related genes, the immune response by lymphocytes, and IFNγ expression were evaluated in virus-infected melanoma cells. The results showed that the rate of rNDV infection varied according to the isolated melanoma cells and the oncolytic effect differed between melanoma cells owing to the infectivity of the virus. The oncolytic effect tended to be greater for the IFNγ-expressing virus than for the GFP-expressing prototype virus. Additionally, lymphocytes co-cultured with the virus showed induced expression of Th1 cytokines. Therefore, recombinant NDV expressing IFNγ is expected to induce cellular immunity and oncolytic activity. This oncolytic treatment shows promise as a therapeutic approach for melanoma treatment once evaluated using clinical samples from humans.

Mosquitoes are an important arthropod vector of arboviruses. Family Phenuiviridae includes several medically important arboviruses, such as Rift Valley fever phlebovirus and Toscana phlebovirus. Recent comprehensive genetic analyses identified many novel mosquito-specific viruses that are phylogenetically related to Phenuiviridae. We collected mosquitoes in Hokkaido, northern Japan, and conducted reverse transcription polymerase chain reactions (RT-PCRs) targeting the RNA-dependent RNA polymerase (RdRp) gene of Phenuiviridae. A total of 285 pools comprising 3,082 mosquitoes from two genera and eight species were collected. Partial RdRp sequences were detected from 97 pools, which allowed us to classify the viruses into three clusters provisionally designated Etutanne virus (ETTV) 1, 2, and 3. The closest related virus was Narangue virus (family Phenuiviridae, genus Mobuvirus), which was detected from Mansonia mosquitoes, and the similarities of its nucleotide and amino acid sequences with those of the ETTVs were 58.4%-66.2% and 64.7%-86.7%, respectively. PCRs and RT-PCRs using DNA and RNase digestion methods showed that the ETTVs were RNA viruses, but they do not form non-retroviral integrated RNA virus sequences in the mosquito genome.

Autoimmune, allergic, and respiratory inflammatory diseases are some of the most important health issues worldwide. Disorders of the gut microbiota have been associated with the induction of allergic and inflammatory diseases, and probiotics are being tested for disease prevention. We examined functional Lactiplantibacillus plantarum RGU (Lp-1) to mice with ovalbumin (OVA)-induced asthma model to elucidate the inhibitory effect on pathological progression in asthma model. Prior to the experiments, the intestinal lactic acid bacteria were reduced by administering multiple antibiotics (MAB) to evaluate the administration effect of lactic acid bacteria. Mice were administered with Lp-1 or comparative control lactic acid bacteria in each group. After that, OVA-induced asthma was induced, and cytokine gene expression and histological findings were compared. Exacerbation of lung lesions was confirmed in the MAB group. The Lp-1 group mice had alleviated lung lesions with a decrease in IL-1β, IL-13, IL-17 and an increase in IL-10 of the splenocytes and bronchial lymph nodes compared with the MAB group, but not in the other groups. In OVA-induced asthma, administration of specific Lactiplantibacillus was confirmed to induce anti-inflammatory cytokines.

The thermal stability of relevant viruses in gelatin liquid formulations for medical research and application is poorly understood. Bovine herpesvirus (BHV) was used as a model virus to examine the molecular weight (MW), concentration and gelatin type and to optimize virus stability in liquid formulations at 25 °C and 4 °C. Using the model virus stable liquid formulation, the stability of multiple enveloped and nonenveloped RNA and DNA viruses, including parainfluenza virus (PIV), reovirus (RV), BHV, and adenovirus (AdV), was monitored over up to a 30-week storage period. The BHV model virus was considered stable after 3 weeks in hydrolyzed gelatin (MW: 4000) with a 0.8 LRV (log10 reduction value) at 25 °C or a 0.2 LRV at 4 °C, compared to the stabilities observed in higher MW gelatin (60000 and 160000) with an LRV above 1. Based on the gelatin type, BHV in B-type gelatin samples were unexpectantly more stable than in A-type gelatin sample. All four viruses exhibited stability at 4 °C for at least 8 weeks, BHV or AdV remained stable for over 30 weeks of storage, and at 25 °C, AdV and RV remained stable for 8 weeks. The results demonstrated that 5% hydrolyzed gelatin can act as a relevant stabilizer for the thermal stability of viruses in medical research and application.

The Gobi Desert is a major source of Asian dust events, and the resulting health hazards have increased significantly in recent years. We reported that a variety of live bacteria were distributed in the Gobi Desert in relation to land use. Bacterial distribution was confirmed in the environment and on the land used by animals, however, bacterial saltation due to dust events has not been investigated in detail. In this study, to understand the distribution of surface bacteria in the atmosphere by dust saltation, live bacteria in four dust-generating areas in the Gobi area were monitored using an artificial dust generating device. The live bacteria were detected by experimental saltation at a wind speed of 6.5–8 m/s in all areas. A certain number of live bacteria are constantly saltated by dust events, and these bacteria depend on land use. Moreover, the bacterial saltation strain depended on land use and diversity, indicating that live bacteria are lifted into the environment by dust events. These findings indicate that dust events saltate environmental bacteria on the ground, suggest the risk of animal-derived bacterial saltation affected by land use, and present cross-border public health challenges to be considered in the future.