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Global economic growth and population expansion contribute to heightened solid waste production, creating environmental challenges. Sustainable waste management, notably incineration, is crucial for volume reduction, energy recovery, and resource utilization. The escalating reliance on waste incineration underlines the critical necessity for detailed waste characterization. Our study focuses on industrial and hazardous incineration residues (IHWA) and provides essential insights into their mineral and chemical composition employing various analytical techniques, including sieve analyses, laser diffraction, X-ray powder diffraction, FT-IR spectroscopy, SEM-EDS, and leaching behavior, thus contributing to filling a gap in the current knowledge. The majority of IHWA particles (41%) were smaller than 500 μm, with a significant portion (34%) in the 200-400 μm range, which increases their leaching potential. Toxic elements, including Cr, Cu, and Zn, were detected at elevated levels, with Zn concentrations exceeding EU soil limits by over 250 times, what underscore environmental risks and demand proper waste treatment. The complexity of mineral composition and bonds within the sample indicates the necessity for a thorough environmental assessment to understand the potential impacts on the ecosystem. Sequential extraction revealed that Zn (90.32%), Cd (64.22%), and Hg were predominantly found in the mobile fractions, indicating a high potential for leaching and environmental hazards. In contrast, elements like Cu, Cr, and Pb were primarily associated with immobile fractions. Mineral phases such as calcite and halite dissolved during the first extraction step, while other crystalline phases, including quartz and magnetite, remained stable throughout the process. The stability of other mineral phases in all results and the low leaching potential of elements in IHWA indicate that some mineral phases could suppress the leaching efficiency of elements., Competing Interests: Declarations. Competing interests: The authors declare no competing interests., (© 2024. The Author(s).)

Introduction: The oriental fruit fly Bactrocera dorsalis is one of the most destructive agricultural pests worldwide, with highly debated species delimitation, origin, and global spread routes., Objectives: Our study intended to (i) resolve the taxonomic uncertainties between B. dorsalis and B. carambolae, (ii) reveal the population structure and global invasion routes of B. dorsalis across Asia, Africa, and Oceania, and (iii) identify genomic regions that are responsible for the thermal adaptation of B. dorsalis., Methods: Based on a high-quality chromosome-level reference genome assembly, we explored the population relationship using a genome-scale single nucleotide polymorphism dataset generated from the resequencing data of 487 B. dorsalis genomes and 25 B. carambolae genomes. Genome-wide association studies and silencing using RNA interference were used to identify and verify the candidate genes associated with extreme thermal stress., Results: We showed that B. dorsalis originates from the Southern India region with three independent invasion and spread routes worldwide: (i) from Northern India to Northern Southeast Asia, then to Southern Southeast Asia; (ii) from Northern India to Northern Southeast Asian, then to China and Hawaii; and (iii) from Southern India toward the African mainland, then to Madagascar, which is mainly facilitated by human activities including trade and immigration. Twenty-seven genes were identified by a genome-wide association study to be associated with 11 temperature bioclimatic variables. The Cyp6a9 gene may enhance the thermal adaptation of B. dorsalis and thus boost its invasion, which tended to be upregulated at a hardening temperature of 38 °C. Functional verification using RNA interference silencing against Cyp6a9, led to the specific decrease in Cyp6a9 expression, reducing the survival rate of dsRNA-feeding larvae exposed to extreme thermal stress of 45 °C after heat hardening treatments in B. dorsalis., Conclusion: This study provides insights into the evolutionary history and genetic basis of temperature adaptation in B. dorsalis., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Production and hosting by Elsevier B.V.)

Sustainable production of pumpkin (Cucurbita maxima Duchesne) partly relies on integrated pest management (IPM) and pollination services. A farmer-managed field study was carried out in Yatta and Masinga Sub-Counties of Machakos County, Kenya, to determine the effectiveness of a recommended IPM package and its interaction with stingless bee colonies (Hypotrigona sp.) for pollinator supplementation (PS). The IPM package comprised Lynfield traps with cuelure laced with the organophosphate malathion, sprays of Metarhizium anisopliae (Mechnikoff) Sorokin isolate ICIPE 69, the most widely used fungal biopesticide in sub-Saharan Africa, and protein baits incorporating spinosad. Four treatments-IPM, PS, integrated pest and pollinator management (which combined IPM and PS), and control-were replicated 4 times. The experiment was conducted in 600 m2 farms in 2 normalized difference vegetation index (NDVI) classes during 2 growing seasons (October 2019-March 2020 and March-July 2020). Fruits showing signs of infestation were incubated for emergence, fruit fly trap catches were counted weekly, and physiologically mature fruits were harvested. There was no effect of IPM, PS, and NDVI on yield across seasons. This study revealed no synergistic effect between IPM and PS in suppressing Tephritid fruit fly population densities and damage. Hypotrigona sp. is not an efficient pollinator of pumpkin. Therefore, we recommend testing other African stingless bees in pumpkin production systems for better pollination services and improved yields., (© The Author(s) 2023. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)

Growing concern over mineral resources supply forces us to search for alternative sources of Phosphorus. The possibility to recover phosphorus from incinerated sewage sludge ashes appears to be an important aspect in anthropogenic phosphorus cycle and sustainable economy. To make phosphorus recovery efficient it is important to learn the chemical and mineral composition of ash and phosphorus speciation. The phosphorus content in the ash was over 7%, what corresponds to medium rich phosphorus ores. The main phosphorus rich mineral phases were phosphate minerals. The most widespread was tri-calcium phosphate Whitlockite with various Fe, Mg and Ca proportions. In minority Fe-PO 4 and Mg-PO 4 were detected. Whitlockite commonly overgrown with hematite, influences negatively mineral solubility and thus recovery potential and indicates low bioavailability of phosphorus. Considerable amount of phosphorus was found in the low crystalline matrix where phosphorus content was around 10 wt% however low crystallinity and dispersed phosphorus also does not strengthen the potential to recover this element., (© 2023. The Author(s).)

A sewage sludge incineration ash contains large amounts of phosphorus, which are considered as a novel anthropogenic waste-based substitute for phosphorus natural resources. Phosphorus is accumulated at most in phosphate minerals of whitlockite structure, that contain Fe, Ca, and Mg and in the matrix composed of Si, Al, Fe, Ca, P, Mg, K, Na in various proportions. The goal of this study was to estimate phosphorus recovery potential. A four-stage sequential extraction, following the modified Golterman procedure, was applied. Separation of four independent fractions enabled to understand better the manner of phosphorus occurrence in the studied ash. The results of the extraction indicated the greatest release of phosphorus combined with organic matter using sulfuric acid. The release was on average at the level of 64%. The chelating Na-EDTA compound indicated lower ability to extract phosphorus (at the level of 35%), and the highest ability to extract heavy metals and potentially toxic elements (As, Zn, Mo). The sequential extraction led to the total recovery of phosphorus of around 40-60., (© 2022. The Author(s).)

Silk sericin (SS) has different physicochemical properties depending on the extraction technique. In this study, SS was isolated in the presence of ingredients, including 5 to 10% ethanol (EtOH) and 5 to 10% glycine. Furthermore, temperature conditions of 80 °C, 100 °C, and 120 °C were used for 1, 3, and 5 h to evaluate the extraction rates. The extraction, gelation, structural, and cytotoxicity properties of SS extracted under different conditions were investigated. Extraction at 100 °C and 120 °C were found to have the highest SS yield, with 80 °C being the lowest. SS isolated at 100 °C and 120 °C for 1 and 3 h in water, and EtOH gelled at 4 °C in 2 to 3 days and 37 °C in 40 min. Glycine SS extracts were obtained at 100 °C and 120 °C for 1 h, gelled at 4 °C for 20 days and 37 °C for 16 h. SS was observed at 80 °C, with no gelation occurring. Glycine SS extracts obtained for 3, and 5 h at 120 °C showed no gelation. Circular dichroism (CD) results show glycine in SS induces α-helix and random coil structure. SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and fast performance liquid chromatography (FPLC) were used to quantify the molecular weight distribution at 63 and 70 kDa, respectively. The MMT assay (3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) revealed no cytotoxicity in macrophage RAW 264.7 cells treated with this method SS; these findings present the significance and possibility of using selected extraction ingredients in SS that allow for the application of native SS at an initial extraction viscosity.