The purpose of this study was to assess the feasibility of testing binocular visual acuity using the automated acuity card procedure (AACP)-a new automated method that uses an eye-tracking system. We included participants aged 5 to 36 months old. Binocular grating acuity was tested using the AACP and Teller Acuity Cards (TACs) with a uniform testing distance (55 cm) in random order. Electronic stimuli in the AACP were similar in size and form to TACII and roughly equivalent to the printed TACII stimuli. The AACP tracked the participant's gaze from the recorded image sequences and automatically determined the grating acuity. Differentiation, correlation, and consistency were compared between the AACP and TACs. Ultimately, 77.11% (155/201) of participants completed both tests. Fewer participants failed the TAC test than the AACP (15 [7.46%] vs. 31 [15.42%]). The average duration of the AACP (median interquartile range [IQR] = 60 [IQR = 41] seconds) was significantly shorter than that of the TAC (median [IQR], 185 [IQR = 66] seconds, p0.001). AACP and TAC visual acuities were robustly correlated (r = 0.83, p0.001). Bland-Altman plots revealed a mean difference between AACP and TAC visual acuities of 0.10 cycles per degree (cpd; 95% limits of agreement = 7.70 cpd). Both the AACP and TACs indicated improved visual acuity with age progression (both, p0.001), with no significant differences between the tests. Electronic stimuli were presented using the AACP yielded clinically useful data on grating acuity in infants and toddlers. AACP performance was comparable to that of TACs, the current clinical gold standard for assessing infant vision regarding testability, reliability, and accuracy.