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Your search keyword '"Jia, Zhong Qiang"' showing total 8 results
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8 results on '"Jia, Zhong Qiang"'

3. Sublethal doses of broflanilide prevents molting in the fall armyworm, Spodoptera frugiperda via altering molting hormone biosynthesis

Author

Jia, Zhong Qiang, Zhan, En Ling, Zhang, Su Gui, Jones, Andrew K., Zhu, Lin, Wang, Ying Nan, Huang, Qiu Tang, Han, Zhao Jun, Zhao, Chun Qing, Jia, Zhong Qiang, Zhan, En Ling, Zhang, Su Gui, Jones, Andrew K., Zhu, Lin, Wang, Ying Nan, Huang, Qiu Tang, Han, Zhao Jun, and Zhao, Chun Qing

Abstract

Broflanilide is a novel insecticide with a unique mode of action on the insect GABA receptor and is registered worldwide for the control of agricultural pests. It shows high efficacy in controlling the fall armyworm (FAW) Spodoptera frugiperda, which is a destructive pest to various crops. FAW were exposed to sublethal concentrations of broflanilide to determine its impact on insect development. Sublethal doses (LD10 and LD30) caused failure of ecdysis, reduced body length of larvae, malformation of pupae, and vestigial wing formation in adults. Also, broflanilide at LD30 significantly reduced the amount of molting hormone (MH) . After exposure to LD10 or LD30 broflanilide, expression of five Halloween genes, which participate in MH biosynthesis, were found to be altered. Specifically, the transcript levels of SfrCYP307A1 (Spook), SfrCYP314A1 (Shade) and SfrCYP315A1 (Shadow) in 3rd day larvae were significantly decreased as well as SfrCYP302A1 (Disembodied) and SfrCYP306A1 (Phantom) in 5th day pupae. In contrast, the transcript levels of SfrCYP302A1 in 3rd day larvae, SfrCYP307A1 and SfrCYP314A1 in 5th day pupae, and SfrCYP306A1, SfrCYP307A1 and SfrCYP315A1 in 0.5th day adults were significantly increased. Our results demonstrate that broflanilide caused the failure of ecdysis in FAW possibly by influencing the intake of cholesterol through inhibition of feeding and also via altering expression of genes important for MH biosynthesis.

Published
2022

4. Broflanilide prolongs the development of fall armyworm Spodoptera frugiperda by regulating biosynthesis of juvenile hormone

Author

Jia Zhong Qiang, Zhan En Ling, Zhang Su Gui, Wang Ying, Song Ping Ping, Jones, Andrew K., Han Zhao Jun, Zhao Chun Qing, Jia Zhong Qiang, Zhan En Ling, Zhang Su Gui, Wang Ying, Song Ping Ping, Jones, Andrew K., Han Zhao Jun, and Zhao Chun Qing

Abstract

Broflanilide, a novel meta-diamide insecticide, has been registered worldwide to control agricultural pests, and cause negative influence to insect under not only lethal but also sublethal level in the field. Fall armyworm (FAW) Spodoptera frugiperda is a worldwide distributed insect frequently controlled by using insecticides, and the potential regulating mechanism of broflanilide on this key insect prompt careful characterization. In the present study, we exposed FAW larvae to sublethal dose (LD5), low lethal doses (LD10 and LD30) and median lethal dose (LD50) of broflanilide and measured various subsequent physiological sublethal effects. FAW larvae body length became shorter (LD5-LD50), the larvae and pupae duration were increased by 0.96-4.63 days (LD5-LD50), and the juvenile hormone (JH) titer significantly increased up to 134.46% (LD10-LD30). Meanwhile, the JH acid methyltransferase gene (JHAMT) and farnesyl diphosphate synthase 1 (FPPS1), which are critical enzymes of JH biosynthesis, increased by 2.07- and 2.18- fold in LD10 broflanilide-treated group, and by 2.22- and 1.78- fold in LD30 broflanilide-treated group in 3rd day larvae of FAW, respectively. In the 12-hour-old adults, SfrFPPS1 increased to 1.92-fold in LD30 broflanilide-treated group. Broflanilide induced multiple physiological sublethal effects on the biosynthesis of JH by regulating the expression of SfrFPPS and SfrJHAMT genes in FAW and likely in other insects (both pests and non-target organisms). Therefore, its potential for Integrated Pest Management should be further assessed.

Published
2022

5. Physiological Function of RDL1 and RDL2 Subunits of the Ionotropic GABA Receptor in the Spodoptera liturawith the CRISPR/Cas9 System In Vivo

Author

Jia, Zhong Qiang, Zhang, Su Gui, Wang, Ying, Pan, Jun Heng, Liu, Fei Fan, Zhan, En Ling, Fouad, Eman Atef, Fu, Ya Li, Pan, Qi Rui, and Zhao, Chun Qing

Abstract

In insect ionotropic γ-aminobutyric acid receptor (iGABAR) subunits, only resistance to dieldrin (RDL) can be individually and functionally expressed in vitro. In lepidopteran, two to three RDL subtypes are identified; however, their physiological roles have not been distinguished in vivo. In this study, SlRdl1 and SlRdl2 of S. liturawere individually knocked out using CRISPR/Cas9, respectively. The mortality and larval and pupal duration of KOSlRdl1 and KOSlRdl2 were increased. The flight time and distance were increased by 43.30%–80.66% and 58.96%–198.22%, respectively, in KOSlRdl1. The GABA-induced current was significantly decreased by 53.57%–74.28% and 46.91%–63.34% in the ventral nerve cord, and the GABA titer was significantly reduced by 17.65%–28.05% and 19.85%–42.46% in KOSlRdl1 and KOSlRdl2, respectively. In conclusion, SlRdl1 and SlRdl2 are necessary for the transmission of GABA-induced neural signals; however, only SlRdl1 could regulate the flight capability of S. litura. Our results provided a new avenue to study lepidopteran iGABARs.

Published
2023
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8. Neuroligin 3 from common cutworm enhances the GABA‐induced current of recombinant SlRDL1 channel.

Author

Wang, Ying, Zhang, Yi‐Chi, Zhang, Ke‐Xin, Jia, Zhong‐Qiang, Tang, Tao, Zheng, Lin‐Lin, Liu, Di, and Zhao, Chun‐Qing

Subjects
CELL adhesion molecules, SPODOPTERA littoralis, GABA receptors, XENOPUS laevis, NEURONS
Abstract

BACKGROUND: Neuroligin (NLG) protein is a nerve cell adhesion molecule and plays a key role in the precision apposition of presynaptic domains on inhibitory and excitatory synapses. Existing studies mainly focused on the function of NLG3 against the excitatory channel. However, the interaction between insect NLG3 and ionotropic GABA receptor, which is the main inhibitory channel, remains unclear. In this study, the Nlg3 of common cutworm (CCW), Spodoptera litura Fabricius, one important agricultural Lepidopteron, is selected to explore its function in the inhibitory channel. RESULTS: The SlNlg3 was obtained and the SlNLG3 contains the characteristic features including transmembrane domain, PDZ‐binding motif and type‐B carboxylesterases signature 2 motif. The SlNlg3 messenger RNA (mRNA) was most abundant in midgut, and exhibited multiple expression patterns in different developmental stages and tissues or body parts. Compared with the single injection of SlRDL1, the median effective concentration value of GABA in activating currents was smaller in Xenopus laevis oocytes co‐injected with SlRDL1 and SlNlg3. In addition, SlNlg3 could enhance the GABA‐induced current of homomeric SlRDL1 channel from −391.86 ± 15.41 to −2152.51 ± 30.09 nA. DsSlNlg3 depressed the expression level of SlNlg3 mRNA more than 64.29% at 6 h. After exposure to median lethal dose of fluralaner, the mortality of CCW injected with dsSlNlg3 was significantly decreased by 13.34% and 30.00% at 24 and 48 h, respectively, compared to injection of dsEGFP. CONCLUSION: NLG3 should have physiological function on ionotropic GABA receptor in vitro, which provided a favorable foundation for further research on the physiological function of Nlg gene in Lepidopteron. © 2021 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Published
2022
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