Search

Although Apiospora Sacc. has previously been considered a sexual morph of Arthrinium species on the basis of phylogenetic, morphological, and ecological diagnoses, a recent study delimited these as different species. Recently, 14 species, including eight new species, of marine Arthrinium have been reported from Korea. Six known species have previously been renamed as species in the genus Apiospora (A. arundinis, A. marii, A. piptatheri, A. rasikravindrae, A. sacchari, and A. saccharicola). However, the eight new species of marine Arthrinium (Ar. agari, Ar. arctoscopi, Ar. fermenti, Ar. koreanum, Ar. marinum, Ar. pusillispermum, Ar. sargassi, and Ar. taeanense) are yet to be studied, and thus the taxonomic status of these species remains to be clarified. In this study, we conducted phylogenetic analyses using the internal transcribed spacer, 28S large subunit ribosomal RNA gene, translation elongation factor 1-alpha, and beta-tubulin regions to confirm the phylogenetic position of these eight species. Based on these analyses, we re-identified the eight Arthrinium species as new combinations in Apiospora. Additionally, among the six known Apiospora species, two (A. piptatheri and A. rasikravindrae) have not previously been recorded in Korea. On the basis of morphological and molecular analyses, we report these as new species in Korea. Herein, we present scanning electron micrographs detailing the morphologies of these species, along with phylogenetic trees and detailed descriptions. [ABSTRACT FROM AUTHOR]

Objective: The biochip method allows microscale multiparametric analysis of macromolecular samples using a matrix of immobilized molecular probes. Selection of materials for biochip fabrication, functionalization of the carrier surface, and the method of immobilization of molecular probes are the key tasks of biochip technology. Methods: Methods of obtaining polymer coating from polyvinyl acetate on the surface of polyethylene terephthalate polymer substrates and subsequent production of brush polymers by photoinduced radical copolymerization of acrylate monomers have been studied. Cell matrices with numerous reactive chemical groups for subsequent immobilization of proteins were formed by photolithography method. Methods of activation of carboxyl groups on brush polymers attached to the surface of polyethylene terephthalate were tested. Immobilization of model protein streptavidin labeled with fluorescent dye Su3 was performed to test the method of activation of carboxyl groups. Results and Discussion: A variant of the immunofluorescence assay in a biological microarray format was tested on the model "streptavidin–biotinylated immunoglobulin." Conclusions: Streptavidin immobilized in brush polymer cells retains functionality and spatial accessibility for binding to biotinylated immunoglobulin and subsequent manifestation by antibodies fluorescently labeled with Cy5 dye, which opens prospects for the use of biological microarrays with brush polymer cells on polyethylene terephthalate substrates for immunofluorescence analysis of various protein targets. [ABSTRACT FROM AUTHOR]

Pathogens can be collected from air and detected in samples by many methods. However, merely detecting pathogens does not answer whether they can spread disease. To fully assess health risks from exposure to airborne pathogens, the infectivity of those agents must be assessed. Air samplers which operate by growing particles through water vapor condensation and subsequently collecting them into a liquid medium have proven effective at conserving the viability of microorganisms. We present a study that assessed performance improvement of one such sampler, BioSpot-GEM™, gained by augmenting it with an upstream virtual impactor (VI) designed to concentrate particles in aerosols. We demonstrate that such an integrated unit improved the collection of live Escherichia coli by a median Concentration Factor (CF) of 1.59 and increased the recovery of viable human coronavirus OC43 (HCoV OC43) by a median CF of 12.7 as compared to the sampler without the VI. Our results also show that OC43 can be concentrated in this way without significant loss of infectivity. We further present that the small BioSpot-GEM™ bioaerosol sampler can collect live E. coli at an efficiency comparable to the larger BioSpot-VIVAS™ bioaerosol sampler. Our analyses show potential benefits toward improving the collection of viable pathogens from the air using a more portable water-based condensation air sampler while also highlighting challenges associated with using a VI with concentrated bioaerosols. This work can aid further investigation of VI usage to improve the collection of pathogens from air, ultimately to better characterize health risks associated with airborne pathogen exposures. Copyright © 2024 American Association for Aerosol Research [ABSTRACT FROM AUTHOR]

Developmental Endothelial Locus-1 (DEL-1), also known as EGF-like repeat and discoidin I-like domain-3 (EDIL3), is increasingly recognized for its multifaceted roles in immunoregulation and vascular biology. DEL-1 is a protein that is mainly produced by endothelial cells. It interacts with various integrins to regulate the behavior of immune cells, such as preventing unnecessary recruitment and inflammation. DEL-1 also helps in resolving inflammation by promoting efferocytosis, which is the process of clearing apoptotic cells. Its potential as a therapeutic target in immune-mediated blood disorders, cardiovascular diseases, and cancermetastasis has been spotlighted due to its wide-ranging implications in vascular integrity and pathology. However, there are still unanswered questions about DEL-1's precise functions and mechanisms. This review provides a comprehensive examination of DEL-1's activity across different vascular contexts and explores its potential clinical applications. It underscores the need for further research to resolve existing controversies and establish the therapeutic viability of DEL-1 modulation. [ABSTRACT FROM AUTHOR]

Liver fibrosis is a reparative response triggered by liver injury. Non‐invasive assessment and staging of liver fibrosis in patients with chronic liver disease are of paramount importance, as treatment strategies and prognoses depend significantly on the degree of fibrosis. Although liver fibrosis has traditionally been staged through invasive liver biopsy, this method is prone to sampling errors, particularly when biopsy sizes are inadequate. Consequently, there is an urgent clinical need for an alternative to biopsy, one that ensures precise, sensitive, and non‐invasive diagnosis and staging of liver fibrosis. Non‐invasive imaging assessments have assumed a pivotal role in clinical practice, enjoying growing popularity and acceptance due to their potential for diagnosing, staging, and monitoring liver fibrosis. In this comprehensive review, we first delved into the current landscape of non‐invasive imaging technologies, assessing their accuracy and the transformative impact they have had on the diagnosis and management of liver fibrosis in both clinical practice and animal models. Additionally, we provided an in‐depth exploration of recent advancements in ultrasound imaging, computed tomography imaging, magnetic resonance imaging, nuclear medicine imaging, radiomics, and artificial intelligence within the field of liver fibrosis research. We summarized the key concepts, advantages, limitations, and diagnostic performance of each technique. Finally, we discussed the challenges associated with clinical implementation and offer our perspective on advancing the field, hoping to provide alternative directions for the future research. [ABSTRACT FROM AUTHOR]

A new high-flow, compact aerosol concentrator, using rapid, turbulent mixing to grow aerosol particles into droplets for dry spot sample collection, has been designed and tested. The "TCAC (Turbulent-mixing, Condensation Aerosol Concentrator)" is composed of a saturator for generating hot vapor, a mixing section where the hot vapor mixes with the cold aerosol flow, a growth tube where condensational droplet growth primarily occurs, and a converging nozzle that focuses the droplets into a beam. The prototype concentrator utilizes an aerosol sample flow rate of 4 L min−1. The TCAC was optimized by varying the operating conditions, such as relative humidity of the aerosol flow, mixing flow ratio, vapor temperature, and impaction characteristics. The results showed that particles with a diameter ≥ 25 nm can be grown to a droplet diameter > 1400 nm with near 100% efficiency. Complete activation and growth were observed at relative humidity ≥ 25% of the aerosol sample flow. A consistent spot sample with a diameter of D90 = 1.4 mm (the diameter of a circle containing 90% of the deposited particles) was obtained regardless of the aerosol particle diameter (dp = 20–1900 nm). For fiber counting applications using phase contrast microscopy, the TCAC can reduce the sampling time, or counting uncertainty, by two to three orders of magnitude, compared to the 25-mm-filter collection. The study shows that the proposed mixing-flow scheme enables a compact spot sample collector suitable for handheld or portable applications, while still allowing for high flow rates. [ABSTRACT FROM AUTHOR]

The current treatment options for peripheral arterial disease (PAD) are limited due to a lack of significant high-level evidence to inform clinical decisions and unfavorable outcomes in terms of cost-effectiveness and amputation rates. In order to suggest the use of the commercially available L-Ornithine-L-Aspartate (LOLA) for treating PAD, we induced hind limb ischemia (HLI) by unilaterally ligating the femoral artery in a rat model. The rats were randomly divided into three groups, with seven rats assigned to each group: group 1 (control), group 2 (sorbitol), and group 3 (LOLA). Intraperitoneal injections were administered five times on post-operative days (PODs) 3, 5, 7, 10, and 12. Perfusion imaging was conducted on PODs 7 and 14 and compared to pre-operative perfusion imaging. Immunohistochemistry staining and Western blotting were performed after the final perfusion imaging. Group 3 showed a significant increase in perfusion, high CD31-positive capillary lumen density, and substantial overexpression of VEGF in the ischemic limb during the subacute phase of HLI. In conclusion, this study provides the first documented evidence of angiogenesis and perfusion recovery in the subacute phase of the HLI model following the administration of LOLA. With LOLA readily available on the commercial market, the implementation of LOLA treatment for PAD in humans can be expedited compared to other therapies still in the developmental stage. [ABSTRACT FROM AUTHOR]

Melt-blown polypropylene (PP) is extensively used in air filtration due to its low cost, low weight, and easy processing, but there are increasing environmental concerns due to its non-degradability. On the other side biodegradable polymers such as polylactic acid (PLA) present insufficient strength and limited toughness. Polymer blending is a well-known approach to reach optimum properties from at least two polymers. This study aims to produce biodegradable PP-PLA-based filter materials that possess enhanced elasticity and superior filtration performance. The addition of PLA raises the average fiber diameter (AFD), causing the PP-PLA filters to have AFD ranging from 0.73 to 0.91 μm. However, the incorporation of zinc stearate (ZnSt) decreased the melt viscosity, resulting in thinner fiber formations with AFD ranging from 0.6 to 0.75 μm for PP-PLA-ZnSt. The efficiency of the corona-charged optimized sample (double-layer 75PP-25PLA-ZnSt) showed 97.42% particle capture efficiency and filtration performance of 0.12 mmH2O−1. Despite the presence of hydrophobic surfaces in all filter materials, the addition of ZnSt further improves the resistance to surface wettability. 75PP-25PLA-ZnSt filter material exhibits high stretchability, with a maximum tensile strength of 380 ± 70 kPa. The proposed tricomponent (PP-PLA-ZnSt) approach would be used to reduce the environmental impact of non-degrading polymers. [ABSTRACT FROM AUTHOR]

Introduction: Image-guided invasive procedures on the liver require a steep learning curve to acquire the necessary skills. The best and safest way to achieve these skills is through hands-on courses that include simulations and phantoms of different complications, without any risks for patients. There are many liver phantoms on the market made of various materials; however, there are few multimodal liver phantoms, and only two are cast in a 3D-printed mold. Methods: We created a virtual liver and 3D-printed mold by segmenting a CT scan. The InVesalius and Autodesk Fusion 360 software packages were used for segmentation and 3D modeling. Using this modular mold, we cast and tested silicone- and gelatin-based liver phantoms with tumor and vascular formations inside. We tested the gelatin liver phantoms for several procedures, including ultrasound diagnosis, elastography, fibroscan, ultrasound-guided biopsy, ultrasound-guided drainage, ultrasound-guided radio-frequency ablation, CT scan diagnosis, CT–ultrasound fusion, CT-guided biopsy, and MRI diagnosis. The phantoms were also used in hands-on ultrasound courses at four international congresses. Results: We evaluated the feedback of 33 doctors regarding their experiences in using and learning on liver phantoms to validate our model for training in ultrasound procedures. Conclusions: We validated our liver phantom solution, demonstrating its positive impact on the education of young doctors who can safely learn new procedures thus improving the outcomes of patients with different liver pathologies. [ABSTRACT FROM AUTHOR]

Background. Using a toxin‐induced lethal acute liver failure (ALF) monkey model, we have recently shown that early peripheral infusion of human umbilical cord mesenchymal stem cells (hUC‐MSCs) can alleviate liver damage and improve animal survival by suppressing the activation of circulating monocytes and the subsequent cytokine storm. Here, we explored whether the administration of hUC‐MSCs could still improve ALF when the cytokine storm is fully developed. Method. We treated ALF monkeys with peripheral delivery of hUC‐MSCs at 48 hr after toxin challenge. Liver indices, histology, imaging, and animal survival were recorded and analyzed. Results. In our cohort study, we conducted and demonstrated that the infusion of hUC‐MSCs significantly improved liver histology, effectively controlled inflammatory cytokine storms, and increased survival rates. Additionally, the administration of a higher dose of hUC‐MSCs (2 × 107/monkey) yielded superior outcomes compared to a lower dose (1 × 107/monkey). Conclusion. Treatment of hUC‐MSCs can significantly improve the pathological and survival outcomes of ALF even when the cytokine storm has been fully developed, indicating a promising clinical solution for ALF. [ABSTRACT FROM AUTHOR]

Although, written corrective feedback (hereafter referred to as CF) is applauded in many writing courses for fostering students' quality writing, its impact on grammatical accuracy in L2 students' writing remains a debated topic. Thus, this study looked into the effect of CF types on L2 students' grammatical accuracy in writing. To achieve this objective, the design of this study was quasi-experiment. During the intervention of the study three groups: two experimental groups and one control group were participated with a total of 150 students. Over the intervention of 8 weeks, the students received pretest, immediate post-test and a delayed post-test was given. The data collected through the writing tests was analyzed using one-way ANOVA and Scheffe post hoc tests. The findings indicated that although CF types have positive effects during the immediate posttest scores, they did not have any positive effect on L2 students' grammatical accuracy in writing context in the delayed posttest scores. This implies that CF alone is not sufficient for improving the grammatical accuracy of high school students in Ethiopia. Although the CF literature discussed its importance in the language acquisition, teachers are advised to focus on students' additional exposures in writing accuracy rather than grammatical correction in their writing classes and use longer treatment to allow language learners' engagement with the CF types provided. [ABSTRACT FROM AUTHOR]

Congenital heart diseases (CHDs) represent a heterogeneous group of congenital defects, with high prevalence worldwide. Non-invasive imaging is essential to guide medical and surgical planning, to follow the patient over time in the evolution of the disease, and to reveal potential complications of the chosen treatment. The application of cardiac magnetic resonance imaging (CMRI) in this population allows for obtaining detailed information on the defects without the necessity of ionizing radiations. This review emphasizes the central role of CMR in the overall assessment of CHDs, considering also the limitations and challenges of this imaging technique. CMR, with the application of two-dimensional (2D) and tri-dimensional (3D) steady-state free precession (SSFP), permits the obtaining of very detailed and accurate images about the cardiac anatomy, global function, and volumes' chambers, giving essential information in the intervention planning and optimal awareness of the postoperative anatomy. Nevertheless, CMR supplies tissue characterization, identifying the presence of fat, fibrosis, or oedema in the myocardial tissue. Using a contrast agent for angiography sequences or 2D/four-dimensional (4D) flows offers information about the vascular, valvular blood flow, and, in general, the cardiovascular system hemodynamics. Furthermore, 3D SSFP CMR acquisitions allow the identification of coronary artery abnormalities as an alternative to invasive angiography and cardiovascular computed tomography (CCT). However, CMR requires expertise in CHDs, and it can be contraindicated in patients with non-conditional devices. Furthermore, its relatively longer acquisition time and the necessity of breath-holding may limit its use, particularly in children under eight years old, sometimes requiring anesthesia. The purpose of this review is to elucidate the application of CMR during the pediatric age. [ABSTRACT FROM AUTHOR]

Background: Patients with liver pathology benefit from image-guided interventions. Training for interventional procedures is recommended to be performed on liver phantoms until a basic proficiency is reached. In the last 40 years, several attempts have been made to develop materials to mimic the imaging characteristics of the human liver in order to create liver phantoms. There is still a lack of accessible, reproducible and cost-effective soft liver phantoms for image-guided procedure training. Methods: Starting from a CT-scan DICOM file, we created a 3D-printed liver mold using InVesalius (Centro de Tecnologia da informação Renato Archer CTI, InVesalius 3 open-source software, Campinas, Brazil) for segmentation, Autodesk Fusion 360 with Netfabb (Autodesk software company, Fusion 360 2.0.19426 with Autodesk Netfabb Premium 2023.0 64-Bit Edition, San Francisco, CA, USA) for 3D modeling and Stratasys Fortus 380 mc 3D printer (Stratasys 3D printing company, Fortus 380 mc 3D printer, Minneapolis, MN, USA). Using the 3D-printed mold, we created 14 gelatin-based liver phantoms with 14 different recipes, using water, cast sugar and dehydrated gelatin, 32% fat bovine milk cream with intravenous lipid solution and technical alcohol in different amounts. We tested all these phantoms as well as ex vivo pig liver and human normal, fatty and cirrhotic liver by measuring the elasticity, shear wave speed, ultrasound attenuation, CT-scan density, MRI signal intensity and fracture force. We assessed the results of the testing performed, as well as the optical appearance on ultrasound, CT and MRI, in order to find the best recipe for gelatin-based phantoms for image-guided procedure training. Results: After the assessment of all phantom recipes, we selected as the best recipe for transparent phantoms one with 14 g of gelatin/100 mL water and for opaque phantom, the recipes with 25% cream. Conclusions: These liver gelatin-based phantom recipes are an inexpensive, reproducible and accessible alternative for training in image-guided and diagnostic procedures and will meet most requirements for valuable training. [ABSTRACT FROM AUTHOR]

This paper describes the development of a dual hazard spectrum for use in the dynamic analysis of steel frames subject to the combined effects of earthquakes and wind. The proposed spectrum is obtained by combining the power spectra of earthquakes and wind using the square root of the sum of squares (SRSS) combination method. An equivalent time excitation function is then computed using an inverse fast Fourier transform (IFFT) and serves as input for the dynamic analysis. Using time-history analysis on the OpenSees platform, the dynamic responses expressed in terms of peak and residual inter-story and roof drifts for two multistory steel frames located in two US cities (Los Angeles and Charleston) are obtained to demonstrate that designing these buildings based on just one hazard may not be adequate. For frames that are considered under-designed, an energy-based design procedure that uses buckling-restrained braces (BRBs) to dissipate the excess energy imparted to these frames is proposed so they will satisfy the FEMA 356 recommended drift limits for the performance levels of immediate occupancy and life safety. [ABSTRACT FROM AUTHOR]

Sweet taste is the most powerful taste modality shaping feeding behavior and influencing homeostasis. This review summarizes data on the reception and encoding of taste signals at the level of taste buds and cerebral centers during the consumption of sweet substances. The main focus of attention is on the molecular and cellular mechanisms underlying identification of sweet taste and detection of the caloric composition of food, including the role of T1R2/T1R3 membrane protein receptors and the associated intracellular enzyme cascade, along with the metabolic mechanism assessing the concentration of glucose entering the cytoplasm. The genetic aspects of sensitivity to sweetness and the influence of sweet taste receptor gene polymorphism on sensitivity to sugars and low-calorie sweeteners are described. We present results from current studies of the endocrine, paracrine, and autocrine modulation of the reception and perception of sweet taste depending on the metabolic status of the body. A suggestion is made regarding a promising direction of research in this area. [ABSTRACT FROM AUTHOR]

(1) Background: Phytochemicals are crucial antioxidants that play a significant role in preventing cancer. (2) Methods: We explored the use of methyl jasmonate (MeJA) in the in vitro cultivation of D. morbifera adventitious roots (DMAR) and evaluated its impact on secondary metabolite production in DMAR, optimizing concentration and exposure time for cost-effectiveness. We also assessed its anti-inflammatory and anti-lung cancer activities and related gene expression levels. (3) Results: MeJA treatment significantly increased the production of the phenolic compound 3,5-Di-caffeoylquinic acid (3,5-DCQA). The maximum 3,5-DCQA production was achieved with a MeJA treatment at 40 µM for 36 h. MeJA-DMARE displayed exceptional anti-inflammatory activity by inhibiting the production of nitric oxide (NO) and reactive oxygen species (ROS) in LPS-induced RAW 264.7 cells. Moreover, it downregulated the mRNA expression of key inflammation-related cytokines. Additionally, MeJA-DMARE exhibited anti-lung cancer activity by promoting ROS production in A549 lung cancer cells and inhibiting its migration. It also modulated apoptosis in lung cancer cells via the Bcl-2 and p38 MAPK pathways. (4) Conclusions: MeJA-treated DMARE with increased 3,5-DCQA production holds significant promise as a sustainable and novel material for pharmaceutical applications thanks to its potent antioxidant, anti-inflammatory, and anti-lung cancer properties. [ABSTRACT FROM AUTHOR]

The increasing prevalence of steatotic liver disease (SLD) in potential living donors is concerning, as it limits donor's availability amid rising demand. OPTIFAST very low-calorie diet (VLCD), a meal replacement product, effectively reduces weight and hepatic steatosis before transplantation. However, data on the outcomes of recipients of VLCD-treated donors are lacking. We conducted a single-center, retrospective study on 199 living donor liver transplant recipients at Toronto General Hospital, Canada, between January 2015 and January 2020. We compared the 1-year posttransplant outcomes between recipients who received organs from donors treated with VLCD (N = 34) for either weight loss or steatosis reduction, with those who did not require treatment (N = 165). Our analysis revealed no statistically significant differences in the rates of postoperative complications (23% vs 32.4%, p = 0.3) or intensive care unit stays (70.9% vs 70.6%, p = 1) between recipients of non-VLCD and VLCD grafts. Following adjusted multivariate logistic regression, receipt of VLCD grafts was not associated with increased hospital length of stay. In addition, one-year mortality did not differ between the two groups (4.2% non-VLCD recipients vs 2.9% VLCD recipients, p = 0.6). OPTIFAST VLCD treatment for liver donors demonstrates positive and safe outcomes in recipients, expanding the pool of potential living donors for increased organ availability. [ABSTRACT FROM AUTHOR]

Installation time for precast concrete (PC) components varies due to component-, location-, and weather-related factors. Because such variability affects the cost, time, and sustainability performance of PC projects, it is important to identify and classify the factors influencing installation time for different types of PC components. Against this background, this study aims to empirically investigate variability in installation time for PC components and different work steps and then identify the factors that affect particular work steps most significantly. To achieve the goal, this study gathered installation time and other influencing factors data from an actual project for 25 days and analyzed the data using a descriptive approach and the recursive feature elimination with cross-validation (RFECV) analysis. The analysis result provides empirical evidence that different PC types and work steps are involved with different levels of variability in installation time. The highest level of variability was shown in PC beams and assembly work. Regarding the factors influencing installation time, it is shown that different work steps are affected by different sets of influencing factors, with temperature and wind speed as the most common influencing factors across different PC types and work steps. This study contributes to the body of knowledge of off-site construction management by offering empirical evidence on the variability in PC installation time and the specific factors affecting PC installation time. [ABSTRACT FROM AUTHOR]

Background: Nicotinamide (NAM+) regulates redox and metabolic activities in the mitochondria. The intention of the research was to identify key genes that relate to nicotinamide in hepatocellular carcinoma (HCC). Methods: Relevant clinical information were collected as well as RNA-seq data using the Cancer Genome Atlas (TCGA) database. Differential analysis was used to discover the genes that were differently expressed. On the key genes associated with NAM, functional enrichment analysis was carried out. Next, receiver operating characteristic (ROC) and prognosis Kaplan-Meier (K-M) curve analyses were used to evaluate the importance of important gene expression, respectively. The immune cell signatures were estimated using the CIBERSORT algorithm. Finally, the anticancer impact of NAM on HCC was experimentally confirmed, and important genes NADSYN1 and NT5C were validated at the protein level in clinical specimens. Results: Six prognostic key genes (NAXE, NADSYN1, NT5C, NT5C3A, PNP and NT5E) were identified. There is an association between the level of key gene expression and the clinical prognosis. Four key genes (NAXE, NADSYN1, NT5C and NT5C3A) have statistical significance of survival prognosis. Finally, the expression of NAM-related genes and the inhibitory effect of NAM on HCC were verified by experiments. Conclusion: The study first found some Nicotinamide metabolism-related differentially expressed genes (NMRDEGs) that are related to HCC can contribute to predicting survival and monitoring the treatment. [ABSTRACT FROM AUTHOR]

[Objective] In recent years, high-yield gas wells, such as those in eastern Sichuan, Tarim Oilfield, and Dongfang Gas in the South China Sea, have been increasingly common. However, these wells are not without their challenges. The production pipe string of Sangao gas wells is prone to vibration failure due to the impact of high-speed gas. Taking the Kuqa high-pressure gas area of the Tarim Oilfield as an example, 19 failure incidents have been recorded out of 15 wells, resulting in huge economic property losses and, regrettably, casualties. The primary cause of these failures has been identified as the excessive dynamic load caused by pipe string vibration. Therefore, understanding the vibration characteristics of the production pipe string has become a crucial study area. The production string in oil and gas wells possesses its unique characteristics. Its large slenderness ratio results in a very low overall stiffness, which is difficult to simulate in a typical small experimental system. [Methods] In this study, we established a test bench for large production string evaluation. We developed an extensive, elongated string model using Polyethylene pipe to replicate the vibration characteristics of the production string under high-speed fluid impact. [Results] The experimental results show that the production string in Sangao gas wells vibrates at a constant frequency under the influence of a high-speed fluid. The vibration amplitude is most pronounced in the middle and bottom parts of the string. It was also observed that an increase in gas yield correlates with an increase in the severity of the pipe column's vibration. An effective method of curtailing this vibration amplitude involves increasing the wall thickness of the tubing. However, it is important to note that while this may reduce the tubing amplitude, it does not change the vibration frequency of the tubing string. The packer installation position also plays a pivotal role in vibration suppression. If the packer is installed too high or too low, it fails to effectively suppress vibration. The optimal placement for a single packer has been found to be at the 8/10 position of the string, where it can efficiently suppress vibration. This paper further delves into the analysis of displacement, frequency, and vibration characteristics of simulated tubing when subjected to a high-speed fluid within the tubes. [Conclusions] This study further explores the effects of pipe wall thickness, gas yield, and packer setting positions on the vibration characteristics of simulated tubing. The following conclusions were drawn from the research. When the fluid Reynolds number within the tube reaches 21 430, the simulated tubing presents a second-order vibration mode. This results in a substantial amplitude in the middle and bottom sections of the simulated tubing, which are prone to contact and collision with the casing wall, potentially resulting in pipe string failure. The wall thickness of the tubing exerts little effect on the vibration frequency of the simulated tubing. However, it significantly affects the vibration amplitude. The thicker the tubing, the smaller the vibration amplitude of the simulated tubing. Therefore, tubing vibration can be effectively suppressed by increasing tubing thickness during the actual production process. The larger the gas production, the greater the frequency and amplitude of the simulated tubing vibration. When gas production is excessively large, the tubing is likely to collide severely with the casing wall, thereby increasing the risk of tubing failure. Therefore, it is crucial in the actual production process to determine the daily gas output reasonably, keeping in mind the bearing limit of the tubing. Packers suppress string vibration by limiting the lateral displacement of the string. Installing multiple packers proves to be more effective than a single packer in suppressing vibration. [ABSTRACT FROM AUTHOR]

BACKGROUND: The increasing prevalence of food sensitivities has been attributed to changes in gut microenvironment; however, ubiquitous environmental triggers such as inorganic nanoparticles (NPs) used as food additives have not been thoroughly investigated. OBJECTIVES: We explored the impact of the NP-structured food-grade silicon dioxide (푓푔-SiO2) on intestinal immune response involved in oral tolerance (OT) induction and evaluated the consequences of oral chronic exposure to this food-additive using a mouse model of OT to ovalbumin (OVA) and on gluten immunopathology in mice expressing the celiac disease risk gene, HLA-DQ8. METHODS: Viability, proliferation, and cytokine production of mesenteric lymph node (MLN) cells were evaluated after exposure to 푓푔-SiO2. C57BL/ 6J mice and a mouse model of OT to OVA were orally exposed to 푓푔-SiO2 or vehicle for 60 d. Fecal lipocalin-2 (Lcn-2), anti-OVA IgG, cytokine production, and immune cell populations were analyzed. Nonobese diabetic (NOD) mice expressing HLA-DQ8 (NOD/DQ8), exposed to 푓푔-SiO2 or vehicle, were immunized with gluten and immunopathology was investigated. RESULTS: MLN cells exposed to 푓푔-SiO2 presented less proliferative T cells and lower secretion of interleukin 10 (IL-10) and transforming growth factor beta (TGF-β) by T regulatory and CD45+ CD11b+ CD103+ cells compared to control, two factors mediating OT. Mice given  푓&#119892-SiO2 exhibited intestinal Lcn-2 level and interferon gamma (IFN-γ) secretion, showing inflammation and less production of IL-10 and TGF-β. These effects were also observed in OVA-tolerized mice exposed to 푓푔-SiO2, in addition to a breakdown of OT and a lower intestinal frequency of T cells. In NOD/DQ8 mice immunized with gluten, the villus-to-crypt ratio was decreased while the CD3+ intraepithelial lymphocyte counts and the Th1 inflammatory response were aggravated after 푓푔-SiO2 treatment. DISCUSSION: Our results suggest that chronic oral exposure to 푓푔-SiO2 blocked oral tolerance induction to OVA, and worsened gluten-induced immunopathology in NOD/DQ8 mice. The results should prompt investigation on the link between SiO2 exposure and food sensitivities in humans. [ABSTRACT FROM AUTHOR]

Hepatocellular carcinoma (HCC) is among the main causes of death by cancer worldwide, representing about 80–90% of all liver cancers. Treatments available for advanced HCC include atezolizumab, bevacizumab, sorafenib, among others. Atezolizumab and bevacizumab are immunological options recently incorporated into first-line treatments, along with sorafenib, for which great treatment achievements have been reached. However, sorafenib resistance is developed in most patients, and therapeutical combinations targeting cancer hallmark mechanisms and intracellular signaling have been proposed. In this review, we compiled evidence of the mechanisms of cell death caused by sorafenib administered alone or in combination with valproic acid and metformin and discussed them from a molecular perspective. [ABSTRACT FROM AUTHOR]

In the context of advancing technological development, chatbots have emerged as an innovative tool in the field of mental health, offering new possibilities to provide therapy and emotional support in an accessible and convenient manner. The aim of this study was to develop and evaluate a chatbot implemented in a web application designed to provide emotional support to an adult population, specifically targeting young people and adults over the age of 18. The research focused on user satisfaction with the chatbot experience. Using a qualitative approach and non-random convenience sampling, we collected feedback on the chatbot’s performance from 15 users through an online questionnaire. The results showed a positive assessment, with an average satisfaction score of 4.09 on a scale of 1 to 5. The participants expressed their approval of the emotional support provided by the chatbot, emphasizing the sense of understanding and trust generated by the therapeutic interventions and emotional support. In conclusion, this study successfully assessed user satisfaction with the emotional support chatbot, emphasizing its significance in the realm of digital mental health. The scope of this study was solely focused on user satisfaction. For future research, it is recommended to expand the scope to investigate the correlation between user satisfaction and therapeutic outcomes. Additionally, there is a need to tailor these systems to meet the specific emotional requirements of diverse user groups and enhance the efficacy of mental health patient care. [ABSTRACT FROM AUTHOR]

Advancements in bioprinting technology are driving the creation of complex, functional tissue constructs for use in tissue engineering and regenerative medicine. Various methods, including extrusion, jetting, and light-based bioprinting, have their unique advantages and drawbacks. Over the years, researchers and industry leaders have made significant progress in enhancing bioprinting techniques and materials, resulting in the production of increasingly sophisticated tissue constructs. Despite this progress, challenges still need to be addressed in achieving clinically relevant, human-scale tissue constructs, presenting a hurdle to widespread clinical translation. However, with ongoing interdisciplinary research and collaboration, the field is rapidly evolving and holds promise for personalized medical interventions. Continued development and refinement of bioprinting technologies have the potential to address complex medical needs, enabling the development of functional, transplantable tissues and organs, as well as advanced in vitro tissue models., (© 2024 Wiley‐VCH GmbH.)

Wearable devices have generally been rigid due to their reliance on silicon-based technologies, while future wearables will utilize flexible components for example transistors within microprocessors to manage data. Two-dimensional (2D) semiconducting flakes have yet to be investigated in fiber transistors but can offer a route toward high-mobility, biocompatible, and flexible fiber-based devices. Here, the electrochemical exfoliation of semiconducting 2D flakes of tungsten diselenide (WSe 2 ) and molybdenum disulfide (MoS 2 ) is shown to achieve homogeneous coatings onto the surface of polyester fibers. The high aspect ratio (>100) of the flake yields aligned and conformal flake-to-flake junctions on polyester fibers enabling transistors with mobilities μ ≈1 cm 2 V -1 s -1 and a current on/off ratio, I on /I off ≈10 2 -10 4 . Furthermore, the cytotoxic effects of the MoS 2 and WSe 2 flakes with human keratinocyte cells are investigated and found to be biocompatible. As an additional step, a unique transistor 'knot' architecture is created by leveraging the fiber diameter to establish the length of the transistor channel, facilitating a route to scale down transistor channel dimensions (≈100 µm) and utilize it to make a MoS 2 fiber transistor with a human hair that achieves mobilities as high as μ ≈15 cm 2 V -1 s -1 ., (© 2024 The Authors. Small Methods published by Wiley‐VCH GmbH.)

In this study, we designed a series of dyes based on the D–π–A scheme, featuring the novel 2-amino pyrrole group, D1, as the donor unit, combined with three different acceptor groups: benzothiadiazole (A2), cyanoacrilic acid, (A3), and a unique pyrimidin-pyridinium salt derivative, A1, not previously employed in DSSC applications. A linear carbon chain (LCC) of varying lengths was used as the π-bridge. We compared the performance of D1 dyes with the well-established electron donor phenothiazine, D2. Spectroscopic, electronic and photochemical properties of these designed compounds were investigated through density functional theory (DFT) and time-dependent density functional theory (TDDFT) simulations. The calculated light-harvesting efficiency (LHE) values showed that A1 yielded the best results, and generally, D1 donors outperformed the reference D2. Additionally, longer LCCs contributed positively to LHE. The study of electron density difference (EDD) maps showed clear charge transfer from the donor unit to the acceptor unit in all cases. Dyes with D1 exhibited lower intramolecular reorganization energy (λi) values, indicating faster charge transfer compared to those with D2. Negative free energy injection values (ΔGinj) were calculated for all sensitizers, signifying efficient electron injection, especially in D2-containing dyes. However, these compounds exhibited positive free energy regeneration values (ΔGreg), implying thermodynamically unfavorable regeneration processes. Conversely, D1-containing compounds demonstrated favorable values for regeneration. Increasing the length of the LCC positively affected ΔGreg but had a less favorable impact on ΔGinj. The calculated charge transfer (qCT) values revealed that the most favorable results occurred when pairing the donor with A1, indicating that this acceptor group promoted charge separation. Simulations of dye@(TiO2)14 anatase complexes indicated that the binding of the designed dyes to the semiconductor improved their transport properties. In summary, all the calculated parameters indicated that D1 possesses superior properties for use in DSSC applications when coupled to A1 and, generally, dyes featuring D1 were predicted to perform well in photovoltaic applications. It is worth noting that ΔGreg values may hinder regeneration in D2-containing dyes, despite their excellent values in other calculated parameters. Our study offers valuable insights for refining dye design in order to boost the performance of dye-sensitized solar cells (DSSCs). [ABSTRACT FROM AUTHOR]

Introduction: E-cigarettes, also referred to as electronic cigarettes, are gaining popularity as an alternative to tobacco use. Although their use has significantly increased recently, there is still much debate over their effectiveness and safety as smoking cessation aids. Objective: This review paper’s objective is to offer an inclusive evaluation of the body of research about the impact of ecigarettes on the oral microbiome and oral health. Methodology: Data was extracted from PubMed, Google Scholar, and Scopus to compile scientific evidence for a narrative review article on the effects of e-cigarettes on the oral microbiome. The investigation was restricted to published research articles from 2001 to 2022, using pre-defined search phrases for e-cigarettes, vaping, quitting smoking, oral microbiota, and oral health. Results: The chemicals in e-cigarettes, including nicotine, propylene glycol, vegetable glycerin, flavorings, and chemical additives, cause a dysbiosis that can lead to a variety of conditions, including dental caries, periodontal diseases, and a variety of mental health issues, such as bone loss, gingival tissue inflammation, and increased plaque accumulation. The chemical toxicity of e-cigarettes, including nicotine and other components present in e-cigarette aerosols, has been implicated in various oral health problems that lead to systemic complications. Conclusion: A wide range of oral health sequelae may be associated with e-cigarette use. Future investigation should focus on elucidating the long-term effects of e-cigarette use on oral microbiomes, and overall health and exploring potential strategies for minimizing the adverse impacts on oral health. [ABSTRACT FROM AUTHOR]

Metastasis accounts for the vast majority of cancer deaths; however, this complex process has yet to be fully explained. To form metastases, cancer cells must undergo a series of steps, known as the "Metastatic cascade", each of which requires a specific functional transformation. Cancer stem cells (CSCs) play a vital role in tumor metastasis, but their dynamic behavior and regulatory mechanisms have not been fully elucidated. Based on the "Metastatic cascade" theory, this review summarizes the effect of liver CSCs on the metastatic biological programs that underlie the dissemination and metastatic growth of cancer cells. Liver CSCs have the capacity to initiate distant organ metastasis via EMT, and the microenvironment transformation that supports the ability of these cells to disseminate, evade immune surveillance, dormancy, and regenerate metastasis. Understanding the heterogeneity and traits of liver CSCs in these processes is critical for developing strategies to prevent and treat metastasis of advanced hepatocellular carcinoma (HCC). [ABSTRACT FROM AUTHOR]

Increased glycolysis is a key characteristic of malignant cells that contributes to their high proliferation rates and ability to develop drug resistance. The glycolysis rate-limiting enzyme hexokinase II (HK II) is overexpressed in most tumor cells and significantly affects tumor development. This paper examines the structure of HK II and the specific biological factors that influence its role in tumor development, as well as the potential of HK II inhibitors in antitumor therapy. Furthermore, we identify and discuss the inhibitors of HK II that have been reported in the literature. [ABSTRACT FROM AUTHOR]

The interconnections between budgets and performance metrics in modern organizations, respectively in higher education institutions (HEIs), stands for an interesting and topical area of interest for both scholars and practitioners. Budget development and execution as well as the analysis of performance indicators can provide a different perspective on how to manage them not only from a short-term financial perspective but also on the overall long-term development capacity. It has manifold organizational and managerial implications, therefore providing insights into the benefits and inherent shortcomings of relying on financial and non-financial performance indicators related to budgets in the educational context emerges as a first step towards an empirical-driven analysis. In this sense, the current study represents a preliminary exploration of the relationships between budgets and performance metrics in HEIs, a first step toward more refined scrutiny. [ABSTRACT FROM AUTHOR]

Edible electronics is emerging in recent years motivated by a diverse number of healthcare applications, where sensors can be safely ingested without the need for any medical supervision. However, the current lack of stable and well-performing edible semiconductors needs to be addressed to reach technological maturity and allow the surge of a new generation of edible circuits. In the quest for good-performing edible semiconductors, this study has explored the possibility of considering materials that are not regulated for intentional ingestion, yet are daily swallowed with no adverse reactions, such as pigments contained in toothpaste. This work first elaborates on the basis of inadvertent ingestion data to estimate the quantity of daily ingested Copper(II) Phthalocyanine (CuPc), a whitening pigment and well-known organic semiconductor. Subsequently, CuPc is employed in the first demonstration of fully edible electrolyte-gated transistors operating at low voltage (<1 V), displaying good reproducibility and stable performance for over a year. The results indicate that, with the daily ingested quantity of CuPc from toothpaste, more than 10 4 edible transistors can be realized, thus paving the way to edible circuits, a critical component of future edible electronic systems., (© 2024 The Author(s). Advanced Science published by Wiley‐VCH GmbH.)

Mesenchymal stem cells have made remarkable progress in recent years. Many studies have reported that human umbilical cord mesenchymal stem cells (hUC-MSCs) have no toxicity, but thromboembolism appeared in patients treated with hUC-MSCs. Therefore, people are still worried about the safety of clinical application. The study aims to determine the safety, potential toxic mechanism and biodistribution of hUC-MSCs. F344RG rats were given 5 or 50 million cells/kg of hUC-MSCs by single administration in compliance with Good Laboratory Practice standards. Standard toxicity was performed. RNA sequencing was then performed to explore the potential toxic mechanisms. In parallel, the biodistribution of hUC-MSCs was examined. The dose of 5 million cells/kg hUC-MSCs had no obvious toxicity on symptom, weight, food intake, hematology, serum biochemistry, urine biochemistry, cytokines, and histopathology. However, blood-tinged secretions in the urethral orifice and 20% mortality occurred at 50 million cells/kg. Disseminated intravascular coagulopathy (DIC) is the leading cause of death. hUC-MSCs significantly upregulated complement and coagulation cascade pathways gene expression, resulting in DIC. Besides, hUC-MSCs upregulated fibrinolytic system suppressor genes A2m, Serping1 and Serpinf2. hUC-MSCs survived in rats for less than 28 days, no hUC-MSC was detected in tissues outside the lungs. There was no toxicity in F344RG rats at 5 million cells/kg, but some toxicities were detected at 50 million cells/kg. hUC-MSCs significantly upregulated complement and coagulation cascade pathways, upregulated the expression of fibrinolytic system suppressor genes A2m, Serping1 and Serpinf2, to inhibit fibrinolytic system, caused DIC, which provided a new insight into the toxic mechanism of hUC-MSCs., (© 2024. The Author(s).)

The accumulation of senescent cells is thought to play a crucial role in aging-associated physiological decline and the pathogenesis of various age-related pathologies. Targeting senescence-associated cell surface molecules through immunotherapy emerges as a promising avenue for the selective removal of these cells. Despite its potential, a thorough characterization of senescence-specific surface proteins remains to be achieved. Our study addresses this gap by conducting an extensive analysis of the cell surface proteome, or "surfaceome", in senescent cells, spanning various senescence induction regimes and encompassing both murine and human cell types. Utilizing quantitative mass spectrometry, we investigated enriched cell surface proteins across eight distinct models of senescence. Our results uncover significant changes in surfaceome expression profiles during senescence, highlighting extensive modifications in cell mechanics and extracellular matrix remodeling. Our research also reveals substantive heterogeneity of senescence, predominantly influenced by cell type and senescence inducer. A key discovery of our study is the identification of four unique cell surface proteins with extracellular epitopes. These proteins are expressed in senescent cells, absent or present at low levels in their proliferating counterparts, and notably upregulated in tissues from aged mice and an Alzheimer's disease mouse model. These proteins stand out as promising candidates for senotherapeutic targeting, offering potential pathways for the detection and strategic targeting of senescent cell populations in aging and age-related diseases., (© 2024 The Author(s). Aging Cell published by Anatomical Society and John Wiley & Sons Ltd.)

Pathological ocular neovascularization resulting from retinal ischemia constitutes a major cause of vision loss. Current anti-VEGF therapies rely on burdensome intravitreal injections of Bevacizumab (Beva). Herein ultrasmall polymeric micelles encapsulating Beva (P@Beva) are developed for noninvasive topical delivery to posterior eye tissues. Beva is efficiently loaded into 11 nm micelles fabricated via self-assembly of hyperbranched amphiphilic copolymers. The neutral, brush-like micelles demonstrate excellent drug encapsulation and colloidal stability. In vitro, P@Beva enhances intracellular delivery of Beva in ocular cells versus free drug. Ex vivo corneal and conjunctival-sclera-choroidal tissues transport after eye drops are improved 23-fold and 7.9-fold, respectively. Anti-angiogenic bioactivity is retained with P@Beva eliciting greater inhibition of endothelial tube formation and choroid sprouting over Beva alone. Remarkably, in an oxygen-induced retinopathy (OIR) model, topical P@Beva matching efficacy of intravitreal Beva injection, is the clinical standard. Comprehensive biocompatibility verifies safety. Overall, this pioneering protein delivery platform holds promise to shift paradigms from invasive intravitreal injections toward simplified, noninvasive administration of biotherapeutics targeting posterior eye diseases., (© 2024 Wiley‐VCH GmbH.)

The genus Cornus (Cornaceae) plants are widely distributed in Europe, southwest Asia, North America, and the mountains of Central America, South America, and East Africa. Cornus plants exhibit antimicrobial, antioxidative, antiproliferative, cytotoxic, antidiabetic, anti-inflammatory, neuroprotective and immunomodulatory activities. These plants are exploited to possess various phytoconstituents such as triterpenoids, iridoids, anthocyanins, tannins and flavonoids. Pharmacological research and clinical investigations on various Cornus species have advanced significantly in recent years. Over the past few decades, a significant amount of focus has also been made into developing new delivery systems for Cornus mas and Cornus officinalis. This review focuses on the morphological traits, ethnopharmacology, phytochemistry, pharmacological activities and clinical studies on extracts and active constituents from plants of Cornus genus. The review also highlights recent novel delivery systems for Cornus mas and Cornus officinalis extracts to promote sustained and targeted delivery in diverse disorders. The overwhelming body of research supports the idea that plants from the genus Cornus have therapeutic potential and can be investigated in the future for treatingseveral ailments., (© 2024 The Authors. Chemistry & Biodiversity published by Wiley-VHCA AG.)

This article proposed a useful simulation to investigate the Liouville-Caputo fractional order pollution model's solution behavior for a network of three lakes connected by channels. A supposedly new approximation technique using the Appell type Changhee polynomials (ACPs) was used to treat the periodic and linear input models. This work employs the spectral collocation method based on the properties of the ACPs. The given technique creates a system of algebraic equations from the studied model. We verified the efficiency of the suggested technique by computing the residual error function. We compared the results to those obtained by the fourth-order Runge-Kutta method (RK4). Our findings confirmed that the technique used provides a straightforward and efficient tool to solve such problems. The key benefit of the suggested method is that it only requires a few easy steps, doesn't produce secular terms and doesn't rely on a perturbation parameter. [ABSTRACT FROM AUTHOR]

Nitric oxide (NO), a free radical labile gas, is involved in the regulation of various biological functions and physiological processes during animal reproduction. Recently, increasing evidence suggests that the biological role and chemical fate of NO is dependent on dynamic regulation of its biosynthetic enzyme, three distinct nitric oxide synthase (NOS) according to their structure, location and function. The impact of NOS isoforms on reproductive functions need to be timely elucidated. Here, we focus on and the basic background and latest studies on the development, structure, importance inhibitor, location pattern, complex functions. Moreover, we summarize the exactly mechanisms which involved some cell signal pathways in the regulation of NOS with cellular and molecular level in the animal reproduction. Therefore, this growing research area provides the new insight into the important role of NOS male and female reproduction system. It also provides the treatment evidence on targeting NOS of reproductive regulation and diseases. [ABSTRACT FROM AUTHOR]

The most common neurodegenerative diseases (NDDs), such as Alzheimer's disease (AD) and Parkinson's disease (PD), are the seventh leading cause of mortality and morbidity in developed countries. Clinical observations of NDD patients are characterized by a progressive loss of neurons in the brain along with memory decline. The common pathological hallmarks of NDDs include oxidative stress, the dysregulation of calcium, protein aggregation, a defective protein clearance system, mitochondrial dysfunction, neuroinflammation, neuronal apoptosis, and damage to cholinergic neurons. Therefore, managing this pathology requires screening drugs with different pathological targets, and suitable drugs for slowing the progression or prevention of NDDs remain to be discovered. Among the pharmacological strategies used to manage NDDs, natural drugs represent a promising therapeutic strategy. This review discusses the neuroprotective potential of seaweed and its bioactive compounds, and safety issues, which may provide several beneficial insights that warrant further investigation. [ABSTRACT FROM AUTHOR]

Background: Tracking the migration pathways of living cells after their introduction into a patient's body is a topical issue in the field of cell therapy. Questions related to studying the possibility of long-term intravital biodistribution of mesenchymal stromal cells in the body currently remain open. Methods: Forty-nine laboratory animals were used in the study. Modeling of local radiation injuries was carried out, and the dynamics of the distribution of mesenchymal stromal cells labeled with [89Zr]Zr-oxine in the rat body were studied. Results: the obtained results of the labelled cell distribution allow us to assume that this procedure could be useful for visualization of local radiation injury using positron emission tomography. However, further research is needed to confirm this assumption. Conclusions: intravenous injection leads to the initial accumulation of cells in the lungs and their subsequent redistribution to the liver, spleen, and kidneys. When locally injected into tissues, mesenchymal stromal cells are not distributed systemically in significant quantities. [ABSTRACT FROM AUTHOR]

In this study, electrospun polyacrylonitrile (PAN)/polypyrrole (PPy) nanofibers (NFs) coated quartz crystal microbalance (QCM) were investigated for their sensing characteristics against six different volatile organic compounds (VOCs): chloroform, dichloromethane, carbon tetrachloride, benzene, toluene and xylene. SEM, TEM, FT-IR and TGA analysis were carried out for the characterization of PAN/PPy nanofibers and characterization results of PAN/PPy NFs showed that these nanofibers were morphologically well-arranged and straightforward with a cylindrical shape with the average fiber diameter of 253.17 ± 27 nm. Among all the gas measurement tests, dichloromethane displayed the highest response values for PAN/PPy coated QCM sensors. When the reproducibility of kinetic studies for PAN/PPy NFs coated QCM sensors were examined, the most repetitive results were obtained by this QCM sensor during dichloromethane investigation and the diffusion coefficients of VOCs for the first and second regions increased with the order of xylene < toluene < benzene < carbontetrachloride < chloroform < dichloromethane. The sensitivities of the PAN/PPy nanofibers-coated QCM sensor against organic vapors are determined between 4.71 and 6.17 (Hz ppm−1) × 10–4. As a result, PAN/PPy nanofibers exhibited high sensitivity and selectivity for VOCs sensor applications, especially for dichloromethane. [ABSTRACT FROM AUTHOR]

The fabrication of a high‐performance PTFE membrane remains challenging and still under interest. Electrospinning is one of the possible fabrication methods for PTFE membranes. But, due to the high solvent resistance and the high melt viscosity, PTFE emulsion should be blended with a carrier polymer solution and then it can be electrospinned. In this study, PTFE membranes were produced with four different ratios of PVA and PEO polymers as carrier and then sintered at 380°C, to left only PTFE nanofibers behind. Produced hydrophobic PTFE membranes' WCA values were between 120‐132° for PEO/PTFE nanofibers and 112‐124° for PVA/PTFE nanofibers. SEM images revealed that PEO/PTFE membranes preserved their fibrous structure better. After sintering, diameter of 7PEO/PTFE, 8PEO/PTFE, 9PEO/PTFE, and 10PEO/PTFE nanofibers were 612, 818, 822, and 923 nm, respectively. Only 30PVA/PTFE nanofibers showed some fiber structure after sintering with evenly distributed pores. PEO/PTFE membranes were thicker than commercial syringe filter membranes and PVA/PTFE membranes and was about 0.24 μm. Although mean pore size measurements of both PEO/PTFE and PVA/PTFE membranes were 0.90 and 1.07 μm, respectively and higher than commercial membranes, bacterial removal tests of PEO/PTFE membrane were promising and close to commercial syringe filter results with 1.3 3log CFU/mL. [ABSTRACT FROM AUTHOR]

The article discusses the book "Legitimacy, Power, and Inequalities in the Multistakeholder Internet Governance: Analyzing IANA Transition" by Nicola Palladino and Mauro Santaniello. The book provides a comprehensive analysis of the IANA Stewardship Transition Process, which aimed to transfer oversight of the Internet Assigned Numbers Authority (IANA) to a more representative group of actors. The authors examine the theoretical framework and technical functioning of internet governance, as well as the complexities and conflicts that arose during the transition process. They argue that the process did not effectively address issues of legitimacy and representation, and instead reinforced the dominance of Western actors in internet governance. The book is recommended for students and researchers interested in internet governance and multistakeholderism. [Extracted from the article]

Diethylnitrosamine (DEN) is found in workplaces, processed meats, tobacco smoke, whiskey, etc. It is capable of forming DNA-adducts. Fluted pumpkin (Telfairia occidentalis [To]) is a medicinal plant, and its herbal preparations have been employed variously in ethnomedicine. Furthermore, it has been reported to possess anti-oxidant, anti-cancer, anti-inflammatory properties. We investigated the possible mitigating effect of the leaf paste of To on DEN-induced deleterious effects in male Wistar rats. Forty-five rats weighing between 100 and 150 g were equally divided into nine groups and treated thus: Group 1 (negative control), Group 2 (0.05 mg/kg carboxymethyl cellulose [CMC] daily), Group 3 (positive control, 25 mg/kg bw DEN administered intraperitoneally thrice per week), Group 4 (25 mg/kg bw quercetin [QUE] daily alone), Groups 5 and 6 (100 and 200 mg/kg bw To daily, respectively), Group 7 (25 mg/kg bw DEN and QUE), Groups 8 and 9 (25 mg/kg bw DEN with 100 and 200 mg/kg bw To, respectively). Blood glucose levels, liver damage biomarkers (aspartate aminotransferase [AST], alanine aminotransferase [ALT] and gamma-glutamyltransferase [γ-GT]), frequency of micronucleated polychromatic erythrocyte (mPCEs), and liver histology were assessed. DEN significantly (p<0.05) increased blood glucose levels, activities of ALT, AST and γ-GT, and frequency of mPCEs. Histologically, DEN caused a severe architectural anarchy. However, the intervention groups demonstrated the remarkable protective properties of To by ameliorating the adverse effects caused by DEN. Taken together, the leaf paste of To is capable of mitigating DEN-induced hepatotoxicity and clastogenicity in male Wistar rats. [ABSTRACT FROM AUTHOR]

Purpose: Cancer is one of the deadliest pathologies with more than 19 million new cases and 10 million cancer-related deaths across the globe. Despite development of advanced therapeutic interventions, cancer remains as a fatal pathology due to lack of early prognostic biomarkers, therapy resistance and requires identification of novel drug targets. Methods: Runt-related transcription factors (Runx) family controls several cellular and physiological functions including osteogenesis. Recent literatures from PubMed was mined and the review was written in comprehensive manner Results: Recent literature suggests that aberrant expression of Runx contributes to tumorigenesis of many organs. Conversely, cell- and tissue-specific tumor suppressor roles of Runx are also reported. In this review, we have provided the structural/functional properties of Runx isoforms and its regulation in context of human cancer. Moreover, in an urgent need to discover novel therapeutic interventions against cancer, we comprehensively discussed the reported oncogenic and tumor suppressive roles of Runx isoforms in several tumor types and discussed the discrepancies that may have risen on Runx as a driver of malignant transformation. Conclusion: Runx may be a novel therapeutic target against a battery of deadly human cancers. [ABSTRACT FROM AUTHOR]