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Background: Watching athletic tournaments induces emotional and physiological responses in sports fans and coaches. The aim of the study was to investigate hormonal and cardiovascular responses in judo coaches observing the fights of their athletes during one-day, high-level tournaments. Material and methods: We studied the biological responses of a group of seventeen judo club coaches between the ages of 39 and 57 years to a one-day judo tournament attended by the adult male and/or female athletes that they coached. At the time of the tournaments and also in neutral conditions, the coaches’ capillary blood and saliva samples were collected concurrently two times a day, both at 7:30 a.m. and after completion of the tournament (at about 8:00 p.m.). Cardiovascular parameters were also determined at the same times of day. Sleep quality (SQ) was assessed on a 6-point scale both on the night preceding the tournament and in neutral conditions. Cortisol (C) and testosterone (T) levels were determined from serum and saliva samples. The results for both days at different times of day were compared. Results: Statistical calculations showed higher concentrations of cortisol and a greater reduction of testosterone levels in serum and saliva at the end of the day during the tournament compared with those on a neutral day. Morning and evening cardiovascular indices were higher during the tournament. Conclusion: The study showed that during one-day tournaments, judo coaches were exposed to stress that induced intermittent hormonal changes in blood and saliva and activated the autonomic nervous system.