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Your search keyword '"Ishii, Kuniaki"' showing total 19 results
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19 results on '"Ishii, Kuniaki"'

3. Midnolin, a Genetic Risk Factor for Parkinson's Disease, Promotes Neurite Outgrowth Accompanied by Early Growth Response 1 Activation in PC12 Cells.

Author

Chiba, Ayano, Kato, Chisato, Nakagawa, Tadashi, Osaki, Tsukasa, Nakamura, Kohei, Norota, Ikuo, Nagashima, Mikako, Hosoi, Toru, Ishii, Kuniaki, and Obara, Yutaro

Subjects
PARKINSON'S disease, TRANSCRIPTION factors, POLYMERASE chain reaction, GENE expression, NEURODEGENERATION
Abstract

Parkinson's disease (PD) is an age-related progressive neurodegenerative disease. Previously, we identified midnolin (MIDN) as a genetic risk factor for PD. Although MIDN copy number loss increases the risk of PD, the molecular function of MIDN remains unclear. To investigate the role of MIDN in PD, we established monoclonal Midn knockout (KO) PC12 cell models. Midn KO inhibited neurite outgrowth and neurofilament light chain (Nefl) gene expression. Although MIDN is mainly localized in the nucleus, it does not encode DNA-binding domains. We therefore hypothesized that MIDN might bind to certain transcription factors and regulate gene expression. Of the candidate transcription factors, we focused on early growth response 1 (EGR1) because it is required for neurite outgrowth and its target genes are downregulated by Midn KO. An interaction between MIDN and EGR1 was confirmed by immunoprecipitation. Surprisingly, although EGR1 protein levels were significantly increased in Midn KO cells, the binding of EGR1 to the Nefl promoter and resulting transcriptional activity were downregulated as measured by luciferase assay and chromatin immunoprecipitation quantitative real-time polymerase chain reaction. Overall, we identified the MIDN-dependent regulation of EGR1 function. This mechanism may be an underlying reason for the neurite outgrowth defects of Midn KO PC12 cells. [ABSTRACT FROM AUTHOR]

Published
2024
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6. Identifying the core concepts of pharmacology education: A global initiative

Author

White, Paul J., primary, Guilding, Clare, additional, Angelo, Tom, additional, Kelly, John P., additional, Gorman, Laurel, additional, Tucker, Steven J., additional, Fun, Ashleigh, additional, Han, Jae, additional, Chen, Guanliang, additional, Samak, Yassmin, additional, Babey, Anna‐Marie, additional, Caetano, Fabiana A., additional, Sarangi, Sudhir Chandra, additional, Koenig, Jennifer, additional, Hao, Haiping, additional, Goldfarb, Joseph, additional, Karpa, Kelly, additional, Vieira, Luciene, additional, Restini, Carolina, additional, Cunningham, Margaret, additional, Aronsson, Patrik, additional, Kelly‐Laubscher, Roisin, additional, Hernandez, Mark, additional, Rangachari, Patangi K., additional, Mifsud, Janet, additional, Mraiche, Fatima, additional, Sabra, Ramzi, additional, Piñeros, Octavio, additional, Zhen, Xuechu, additional, Kwanashie, Helen, additional, Exintaris, Betty, additional, Karunaratne, Nilushi, additional, Ishii, Kuniaki, additional, and Liu, Yannee, additional

Published
2023
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10. Identifying the core concepts of pharmacology education: a global initiative

Author

White, Paul, primary, Guilding, Clare, additional, Angelo, Tom, additional, Kelly, John, additional, Gorman, Laurel, additional, Tucker, Steve, additional, Fun, Ashleigh, additional, Han, Jae, additional, Chen, Guanliang, additional, Samak, Yassmin, additional, Babey, Anna-Marie, additional, Caetano, Fabiana, additional, Sarangi, Sudhir, additional, Keonig, Jennifer, additional, Hao, Haiping, additional, Goldfarb, Joseph, additional, Karpa, Kelly, additional, Vieira, Luciene, additional, Restini, Carolina, additional, Cunningham, Margaret, additional, Aronsson, Patrik, additional, Kelly-Laubscher, Roisin, additional, Hernandez, Mark, additional, Rangachari, PK, additional, Mifsud, Janet, additional, Mraiche, Fatima, additional, Sabra, Ramzi, additional, Piñeros, Octavio, additional, Zhen, Xuechu, additional, Kwanashie, Helen, additional, Exintaris, Betty, additional, Karunaratne, Nilushi, additional, and Ishii, Kuniaki, additional

Published
2022
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16. JCS/JHRS 2020 Guideline on Pharmacotherapy of Cardiac Arrhythmias.

Author

Ono, Katsushige, Iwasaki, Yu‐ki, Akao, Masaharu, Ikeda, Takanori, Ishii, Kuniaki, Inden, Yasuya, Kusano, Kengo, Kobayashi, Yoshinori, Koretsune, Yukihiro, Sasano, Tetsuo, Sumitomo, Naokata, Takahashi, Naohiko, Niwano, Shinichi, Hagiwara, Nobuhisa, Hisatome, Ichiro, Furukawa, Tetsushi, Honjo, Haruo, Maruyama, Toru, Murakawa, Yuji, and Yasaka, Masahiro

Subjects
PREVENTION of drug side effects, ARRHYTHMIA prevention, ARRHYTHMIA diagnosis, MYOCARDIAL depressants, CARDIOLOGY, ANTICOAGULANTS, MEDICAL protocols, ARRHYTHMIA, PHARMACODYNAMICS, DISEASE risk factors
Published
2022
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17. JCS/JHRS2020 Guideline on Pharmacotherapy of Cardiac Arrhythmias

Author

Ono, Katsushige, Iwasaki, Yu‐ki, Akao, Masaharu, Ikeda, Takanori, Ishii, Kuniaki, Inden, Yasuya, Kusano, Kengo, Kobayashi, Yoshinori, Koretsune, Yukihiro, Sasano, Tetsuo, Sumitomo, Naokata, Takahashi, Naohiko, Niwano, Shinichi, Hagiwara, Nobuhisa, Hisatome, Ichiro, Furukawa, Tetsushi, Honjo, Haruo, Maruyama, Toru, Murakawa, Yuji, Yasaka, Masahiro, Watanabe, Eiichi, Aiba, Takeshi, Amino, Mari, Itoh, Hideki, Ogawa, Hisashi, Okumura, Yasuo, Aoki‐Kamiya, Chizuko, Kishihara, Jun, Kodani, Eitaro, Komatsu, Takashi, Sakamoto, Yusuke, Satomi, Kazuhiro, Shiga, Tsuyoshi, Shinohara, Tetsuji, Suzuki, Atsushi, Suzuki, Shinya, Sekiguchi, Yukio, Nagase, Satoshi, Hayami, Noriyuki, Harada, Masahide, Fujino, Tadashi, Makiyama, Takeru, Maruyama, Mitsunori, Miake, Junichiro, Muraji, Shota, Murata, Hiroshige, Morita, Norishige, Yokoshiki, Hisashi, Yoshioka, Koichiro, Yodogawa, Kenji, Inoue, Hiroshi, Okumura, Ken, Kimura, Takeshi, Tsutsui, Hiroyuki, and Shimizu, Wataru

Published
2022
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18. Preferential Expression of Ca 2+ -Stimulable Adenylyl Cyclase III in the Supraventricular Area, including Arrhythmogenic Pulmonary Vein of the Rat Heart.

Author

Okamoto, Yosuke, Aung, Naing Ye, Tanaka, Masahiro, Takeda, Yuji, Takagi, Daichi, Igarashi, Wataru, Ishii, Kuniaki, Yamakawa, Mitsunori, and Ono, Kyoichi

Subjects
ADENYLATE cyclase, HEART, PULMONARY veins, ATRIAL fibrillation, SOLAR cells, RATS, WESTERN immunoblotting
Abstract

Ectopic excitability in pulmonary veins (PVs) is the major cause of atrial fibrillation. We previously reported that the inositol trisphosphate receptor in rat PV cardiomyocytes cooperates with the Na+-Ca2+ exchanger to provoke ectopic automaticity in response to norepinephrine. Here, we focused on adenylyl cyclase (AC) as another effector of norepinephrine stimulation. RT-PCR, immunohistochemistry, and Western blotting revealed that the abundant expression of Ca2+-stimulable AC3 was restricted to the supraventricular area, including the PVs. All the other AC isotypes hardly displayed any region-specific expressions. Immunostaining of isolated cardiomyocytes showed an enriched expression of AC3 along the t-tubules in PV myocytes. The cAMP-dependent response of L-type Ca2+ currents in the PV and LA cells is strengthened by the 0.1 mM intracellular Ca2+ condition, unlike in the ventricular cells. The norepinephrine-induced automaticity of PV cardiomyocytes was reversibly suppressed by 100 µM SQ22536, an adenine-like AC inhibitor. These findings suggest that the specific expression of AC3 along t-tubules may contribute to arrhythmogenic automaticity in rat PV cardiomyocytes. [ABSTRACT FROM AUTHOR]

Published
2022
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19. JCS/JHRS 2020 Guideline on Pharmacotherapy of Cardiac Arrhythmias.

Author

Ono K, Iwasaki YK, Akao M, Ikeda T, Ishii K, Inden Y, Kusano K, Kobayashi Y, Koretsune Y, Sasano T, Sumitomo N, Takahashi N, Niwano S, Hagiwara N, Hisatome I, Furukawa T, Honjo H, Maruyama T, Murakawa Y, Yasaka M, Watanabe E, Aiba T, Amino M, Itoh H, Ogawa H, Okumura Y, Aoki-Kamiya C, Kishihara J, Kodani E, Komatsu T, Sakamoto Y, Satomi K, Shiga T, Shinohara T, Suzuki A, Suzuki S, Sekiguchi Y, Nagase S, Hayami N, Harada M, Fujino T, Makiyama T, Maruyama M, Miake J, Muraji S, Murata H, Morita N, Yokoshiki H, Yoshioka K, Yodogawa K, Inoue H, Okumura K, Kimura T, Tsutsui H, and Shimizu W

Subjects
Humans, Arrhythmias, Cardiac drug therapy
Published
2022
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