13 results on '"IZUMI KINOSHITA"'
Search Results
2. Histological and immunohistochemical features and genetic alterations in the malignant progression of giant cell tumor of bone: a possible association with TP53 mutation and loss of H3K27 trimethylation
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Makoto Endo, Hidetaka Yamamoto, Shin Ishihara, Kengo Kawaguchi, Yoshihiro Matsumoto, Yuichi Yamada, Yasuharu Nakashima, Kenichi Kohashi, Yoshihiro Ito, Toshifumi Fujiwara, Izumi Kinoshita, Yuko Kakuda, Yousuke Susuki, Takeo Yamamoto, Nokitaka Setsu, Masato Yoshimoto, Yoshinao Oda, Yu Toda, and Takeshi Iwasaki
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Giant Cell Tumor of Bone ,Pathology ,medicine.medical_specialty ,medicine.diagnostic_test ,EZH2 ,Bone Neoplasms ,Sarcoma ,Biology ,medicine.disease ,Methylation ,Pathology and Forensic Medicine ,Malignant transformation ,Histones ,Histone ,Mutation ,Histone methylation ,medicine ,biology.protein ,Humans ,Immunohistochemistry ,Tumor Suppressor Protein p53 ,Gene ,In Situ Hybridization, Fluorescence ,Fluorescence in situ hybridization ,Giant-cell tumor of bone - Abstract
In rare cases, giant cell tumor of bone (GCTB) can undergo primary or secondary malignant transformation to malignant giant cell tumor of bone (MGCTB), but the details of the molecular alterations are still unclear. The present study aimed to elucidate the clinicopathologic and molecular features of MGCTBs based on immunohistochemistry, fluorescence in situ hybridization (FISH) and next generation sequencing (NGS) of nine MGCTBs (five primary and four secondary). Seven (78%) of 9 MGCTBs were immunohistochemically positive for H3.3 G34W. In two (22%) patients, although GCTB components were focally or diffusely positive for H3.3 G34W, their malignant components were entirely negative for H3.3 G34W, which was associated with heterozygous loss of H3F3A by FISH. NGS on four MGCTBs revealed pathogenic mutations in TP53 (n = 3), EZH2 (n = 1) and several other genes. Immunohistochemical analysis of the nine MGCTBs confirmed the p53 nuclear accumulation (n = 5) and loss of H3K27me3 expression (n = 3) and showed that they were mutually exclusive. In addition, four (80%) of five cases of pleomorphic or epithelioid cell-predominant MGCTBs were positive for p53, while three (75%) of four cases of spindle cell-predominant MGCTBs were negative for trimethylation at lysine 27 of histone 3 (H3K27me3). The results suggested that p53 alteration and dysfunction of histone methylation as evidenced by H3K27me3 loss may play an important role in the malignant progression of GCTB, and might contribute to the phenotype-genotype correlation in MGCTB. The combined histologic, immunohistochemical and molecular information may be helpful in part for the diagnosis of challenging cases.
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- 2022
3. Cytology of gynecologic perivascular epithelioid cell tumor (PEComa) with different clinical features
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Takuya ABE, Takeshi IWASAKI, Keiichiro IDE, Tomomi TATEIWA, Manabu OKUZONO, Noriko TOYOSHIMA, Takakazu SASAGURI, Izumi KINOSHITA, and Junichi MOTOSHITA
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- 2022
4. Downstream drifting of Macrobrachium (Decapoda: Palaemonidae) larvae in the Shimanto River, Japan
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Takahiro Kusaka, Shinji Fujita, Hiroyuki Hiraga, Izumi Kinoshita, and Kensaku Azuma
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Larva ,Macrobrachium ,Ecology ,biology ,Downstream (manufacturing) ,Decapoda ,Zoology ,Aquatic Science ,Oceanography ,biology.organism_classification ,Palaemonidae ,Ecology, Evolution, Behavior and Systematics - Published
- 2021
5. Comparison of larval distribution in two clupeoid fishes (Ilisha elongata and Sardinella zunasi) in the inner estuaries of Ariake Sound, Shimabara Bay, Japan
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Yuta Yagi, Yuichi Hirota, Shotaro Tojima, Shinji Fujita, Izumi Kinoshita, and Xiaodong Wang
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Larva ,geography ,geography.geographical_feature_category ,Ecology ,biology ,business.industry ,Distribution (economics) ,Estuary ,Aquatic Science ,Oceanography ,biology.organism_classification ,Fishery ,Ilisha elongata ,Sardinella zunasi ,business ,Bay ,Ecology, Evolution, Behavior and Systematics ,Sound (geography) - Published
- 2021
6. Expression of SATB2, RUNX2, and SOX9 and possible osteoblastic and chondroblastic differentiation in chondroblastoma
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Yu Toda, Hidetaka Yamamoto, Takeshi Iwasaki, Shin Ishihara, Yoshihiro Ito, Yosuke Susuki, Kengo Kawaguchi, Izumi Kinoshita, Daisuke Kiyozawa, Yuichi Yamada, Kenichi Kohashi, Atsushi Kimura, Toshifumi Fujiwara, Nokitaka Setsu, Makoto Endo, Yoshihiro Matsumoto, Yasuharu Nakashima, Masaaki Mawatari, and Yoshinao Oda
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Cell Biology ,Pathology and Forensic Medicine - Abstract
Chondroblastoma (CB) is histologically characterized by oval to polygonal-shaped mononuclear neoplastic cells, multinucleated osteoclastic giant cells, and eosinophilic matrix with occasional calcification. Genetically, the majority of CBs harbor H3F3B p.K36M mutation. Despite the historical nomenclature, it has been reported that the matrix of CB is similar to osteoid rather than true cartilage; however, it remains unclear whether neoplastic cells in CB have the potential for osteoblastic differentiation. To clarify this issue, we immunohistochemically examined the expression of osteogenic and chondrogenic markers (SATB2, RUNX2, p63, and SOX9) as well as H3K36M mutant protein in 33 cases of CB. All 33 cases of CB were positive for H3K36M, while SATB2, RUNX2, p63, and SOX9 were expressed in 30/33 (91%), 33/33 (100%), 29/33 (88%), and 31/32 (97%) CB cases, respectively. Our immunohistochemical results suggest that neoplastic cells in CB frequently express both osteogenic and chondrogenic markers and may have an intermediate feature of osteoblastic and chondroblastic nature.
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- 2022
7. Prognostic implications of the immunohistochemical expression of perilipin 1 and adipophilin in high-grade liposarcoma
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Kengo Kawaguchi, Kenichi Kohashi, Taro Mori, Hidetaka Yamamoto, Takeshi Iwasaki, Izumi Kinoshita, Yosuke Susuki, Hiroshi Furukawa, Makoto Endo, Yoshihiro Matsumoto, Yasuharu Nakashima, and Yoshinao Oda
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General Medicine ,Pathology and Forensic Medicine - Abstract
AimsLiposarcoma is a malignant soft tissue tumour with adipocytic differentiation. Dedifferentiated liposarcoma (DDLS) and myxoid liposarcoma (MLS) are classified as high-grade liposarcomas. Lipid droplet-associated protein (also known as perilipin 1 (PLIN1)) is the predominant perilipin and has utility as a specific marker of adipogenic differentiation. Adipose differentiation-related protein (also known as adipophilin (ADRP)) is ubiquitously expressed in a range of tissues. High ADRP expression is reportedly a poor prognostic factor in several cancer types. However, no previous studies have examined the association between PLIN1 or ADRP expression and prognosis in sarcoma. This study therefore aimed to evaluate the association between PLIN1 or ADRP expression and prognosis in liposarcoma.MethodsIn total, 97 primary resection specimens (53 MLS and 44 DDLS) were examined in this study. PLIN1 and ADRP expression was evaluated by immunohistochemistry. Survival analyses were performed for MLS and DDLS.ResultsOf the 53 MLS specimens, 15 (28.3%) exhibited high PLIN1 expression. PLIN1 expression was not observed in DDLS specimens. High PLIN1 expression was significantly associated with increased disease-free survival (DFS) among patients with MLS (p=0.045). Distinct ADRP expression was observed in 13 of 53 (24.5%) MLS specimens and 5 of 44 (11.4%) DDLS specimens. High ADRP expression was associated with shorter overall survival (OS) in MLS (p=0.042) and DFS and shorter OS in DDLS (p=0.024 and pConclusionsPLIN1 and ADRP expression is associated with poor prognosis in high-grade liposarcoma.
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- 2023
8. A new scoring system for the grading of conventional chondrosarcoma: Its clinicopathological significance
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Yosuke Susuki, Yuichi Yamada, Yoshihiro Ito, Kengo Kawaguchi, Hiroshi Furukawa, Kenichi Kohashi, Izumi Kinoshita, Kenichi Taguchi, Yasuharu Nakashima, and Yoshinao Oda
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Cell Biology ,Pathology and Forensic Medicine - Abstract
Chondrosarcoma is the second most common primary malignant bone tumor, which produces cartilaginous matrix without neoplastic osteoid or bone formation. The histological grade in the WHO Classification of Soft Tissue and Bone (2020 edition) is the most important factor in predicting the clinical outcome of conventional chondrosarcoma, but the lack of clarity in its detailed definition is occasionally problematic. Here, we reviewed conventional chondrosarcoma cases and validated the significance of histological findings. Moreover, we proposed a new scoring system of conventional chondrosarcoma.Clinicopathological features of 60 cases of conventional chondrosarcoma and 21 cases of dedifferentiated chondrosarcoma were reviewed.Moderate to severe nuclear atypia was correlated with distant metastasis. Moderate and severe nuclear atypia, high cellularity, and1 % myxoid change were correlated with adverse overall survival. On the other hand, cases with mild nuclear atypia showed no tumor-related death and no metastases. Based on the above results, we proposed a new scoring system based on nuclear atypia (mild: 0, moderate: +1, severe: +2), cellularity (no and mildly increased cellularity: 0, moderately and diffusely increased cellularity: +1), necrosis [(-): 0, (+): + 1], and chondromyxoid area [(-): 0, (+): + 1]. Each grade was defined as follows: cases with only mild nuclear atypia as grade 1, cases with total score 1-3 excluding mild nuclear atypia as grade 2, and cases with total score 4 or 5 as grade 3. There were 18 cases (30 %) of grade 1 including 5 cases (28 %) of local recurrence, but no metastasis or tumor-related death; 26 cases (43 %) of grade 2 including 2 cases (8 %) of local recurrence, 3 cases (12 %) of metastasis, and 1 case (4 %) of tumor-related death; and 16 cases (27 %) of grade 3 including 4 cases (25 %) of local recurrence, 6 cases (38 %) of metastasis, and 5 cases (31 %) of tumor-related death. There was no statistically significant association between the histological findings and dedifferentiation.From this study, we propose a new histological scoring system for the grading of conventional chondrosarcoma, based on nuclear atypia, cellularity, necrosis, and myxoid change. Using this system, conventional chondrosarcoma may be clearly classified into three grades: grade 1, non-metastasizing; grade 2, metastasizing but rarely life-threatening; and grade 3, frequently metastasizing and life-threatening.
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- 2022
9. Clinicopathological and histopathological review of dedifferentiated liposarcoma: a comprehensive study of 123 primary tumours
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Yoshihiro Ito, Yoshinao Oda, Taro Mori, Kenichi Kohashi, Hidetaka Yamamoto, Yasuharu Nakashima, Yuichi Yamada, Kengo Kawaguchi, Yosuke Susuki, and Izumi Kinoshita
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Oncology ,Male ,medicine.medical_specialty ,Dedifferentiated liposarcoma ,Multivariate analysis ,Histology ,Mitotic Count ,Pathology and Forensic Medicine ,Internal medicine ,Overall survival ,medicine ,Pathology ,Humans ,Neoplasm Metastasis ,Preoperative treatment ,Aged ,Univariate analysis ,business.industry ,Distant metastasis ,Proto-Oncogene Proteins c-mdm2 ,General Medicine ,Liposarcoma ,Middle Aged ,Prognosis ,Survival Rate ,Female ,Neoplasm Recurrence, Local ,business - Abstract
Dedifferentiated liposarcoma (DDLS) has varying histopathological features, but their significance for the biological behaviour of this disease has not been fully clarified. The aim of this study was to elucidate the prognostic factors for DDLS by clinicopathologically reviewing a large case series.We clinicopathologically reviewed 123 cases of primary de-novo DDLS without preoperative treatment, including 81 in the internal trunk (internal DDLS) and 42 in peripheral sites (peripheral DDLS). Univariate and multivariate analyses of their features were also performed for all cases, the internal DDLS group, and the peripheral DDLS group. The results showed that, in all three groups, distant metastasis was significantly associated with shorter overall survival (OS) (univariate analysis, P 0.0001, P = 0.0011, and P = 0.0101, respectively), whereas local recurrence showed no significant effect on prognosis. Histopathologically, a high mitotic count and the presence of round tumour cells were significantly associated with shorter OS in multivariate analysis of the internal DDLS group [respectively: P = 0.0022, hazard ratio (HR) 4.39, 95% confidence interval (CI) 1.71-11.28; and P = 0.0014, HR 7.19, 95% CI 2.14-24.16]. In the peripheral DDLS group, necrosis and high-grade histological components were significantly associated with shorter OS (univariate analysis, P = 0.0068 and P = 0.0174, respectively).The presence of round tumour cells may be one of the histological factors associated with a worse prognosis of DDLS patients, as previous studies indicated. This study also suggests that distant metastasis may be predictive of prognosis for both internal and peripheral DDLS, rather than local recurrence.
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- 2021
10. Histological background of dedifferentiated solitary fibrous tumour.
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Yuichi Yamada, Kenichi Kohashi, Izumi Kinoshita, Hidetaka Yamamoto, Takeshi Iwasaki, Masato Yoshimoto, Shin Ishihara, Yu Toda, Yoshihiro Ito, Yuki Kuma, Yui Yamada-Nozaki, Yutaka Koga, Mikiko Hashisako, Daisuke Kiyozawa, Daichi Kitahara, Fumiya Narutomi, Yusuke Kuboyama, Takahito Nakamura, Takeshi Inoue, and Munenori Mukai
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PLEURA ,CELL cycle proteins ,TUMORS - Published
- 2022
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11. Prognostic implication of desmoplastic stroma in synovial sarcoma: A histological review
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Toshifumi Fujiwara, Shin Ishihara, Daisuke Kiyozawa, Yuichi Yamada, Yu Toda, Yuki Tateishi, Yosuke Susuki, Yoshihiro Matsumoto, Taro Mori, Masaaki Mawatari, Yasuharu Nakashima, Kenichi Kohashi, Izumi Kinoshita, Nokitaka Setsu, Kengo Kawaguchi, Hidetaka Yamamoto, Yoshihiro Ito, Makoto Endo, Yusuke Kuboyama, and Yoshinao Oda
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Adult ,Male ,Prognostic factor ,Pathology ,medicine.medical_specialty ,Adolescent ,Cancer associated fibroblast ,Soft Tissue Neoplasms ,Pathology and Forensic Medicine ,Fusion gene ,Sarcoma, Synovial ,Young Adult ,Stroma ,Humans ,Medicine ,Child ,Aged ,Retrospective Studies ,Aged, 80 and over ,Sclerosis ,business.industry ,Distant metastasis ,Malignant Soft Tissue Neoplasm ,Cell Biology ,Middle Aged ,Prognosis ,medicine.disease ,Predictive value ,Synovial sarcoma ,Extracellular Matrix ,Female ,Collagen ,business - Abstract
Synovial sarcoma (SS) is a malignant soft tissue neoplasm harboring SS18-SSX fusion gene and is histologically characterized by spindle cells and epithelial components. Some investigations have demonstrated that desmoplastic reaction (DR) is an independent prognostic factor of cancers. However, it remains unknown whether DR is of predictive value for the prognosis of synovial sarcoma patients. Here, we reviewed the clinical and histological findings of 88 patients with SS. We defined DR as hyalinized collagenous structures and classified the degree of DR as follows: none, mild, moderate, and severe. Overall, 23 SS cases (24%) showed moderate or severe DR histologically. Statistically, the cases with moderate or severe degree of DR showed poorer prognosis than those with no or mild DR (local recurrence: P = 0.0059, distant metastasis: P = 0.0002, tumor death: P = 0.0382). The findings of the study suggest that the DR of synovial sarcoma could be an important prognostic factor.
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- 2021
12. Downstream drifting of Macrobrachium (Decapoda: Palaemonidae) larvae in the Shimanto River, Japan.
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HIROYUKI HIRAGA, KENSAKU AZUMA, TAKAHIRO KUSAKA, IZUMI KINOSHITA, and SHINJI FUJITA
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MACROBRACHIUM ,LARVAE ,BRACKISH waters ,FRESH water ,EGG yolk ,DECAPODA - Abstract
This study was undertaken to clarify the downstream drifting of Macrobrachium larvae in the Shimanto River by 24-hour sampling. Stage I zoeae accounted for most larvae collected in fresh and brackish waters. M. formosense and M. japonicum dominated in brackish and fresh waters, respectively, while M. nipponense occurred only in brackish waters. The amount of yolk droplets of M. formosense and M. japonicum reduced over time in freshwater. However, no larvae that had completely used up their yolks were observed. Even though M. japonicum larvae drifted from farther upstream than those of M. formosense, they had a higher residual amount of yolk droplets. Therefore, long-distance and long-time drifting in M. japonicum may be enabled by delaying the absorption rate of droplets. [ABSTRACT FROM AUTHOR]
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- 2021
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13. Comparison of larval distribution in two clupeoid fishes (Ilisha elongata and Sardinella zunasi) in the inner estuaries of Ariake Sound, Shimabara Bay, Japan.
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XIAODONG WANG, YUTA YAGI, SHOTARO TOJIMA, IZUMI KINOSHITA, SHINJI FUJITA, and YUICHI HIROTA
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LIFE history theory ,FISHING nets ,SPECIES distribution ,LARVAE - Abstract
In early life history, the clupeoid fishes, Ilisha elongata (Pristigasteridae) and Sardinella zunasi (Clupeidae) have similar spawning seasons and nurseries from spring to summer in the Ariake Sound, Shimabara Bay, Japan. Surveys conducted using larva nets and a beam trawl in July showed that the horizontal egg and larva distributions of I. elongata and S. zunasi both overlapped considerably in the inner estuaries; however, I. elongata settled in the estuaries while S. zunasi retained a pelagic distribution. A comparison of the vertical distributions of the two species in the estuary showed that I. elongata larvae were dispersed from the surface to near-bottom layers during the flood tide, and moved downwards to avoid being swept out of the estuary after the high tide, while S. zunasi larvae remained spread throughout the water column in all tidal phases. The findings suggest the existence of niche segregation during the pelagic larval period in the two species. Thus, by examining differences in larval mouth sizes, which are much larger in I. elongata than in S. zunasi, it appears that prey preference varies between the two species, prey specialization occurs, and there is no niche competition between the two species. [ABSTRACT FROM AUTHOR]
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- 2021
- Full Text
- View/download PDF
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