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Constantly examining novel alternative materials to improve devices performance is a pressing issue in light of the scaling constraints of siliconbased metal-oxide-semiconductor field-effect transistors (MOSFETs). Two-dimensional (2D) aluminum nitride (AlN) with constancy under ambient settings is a suitable channel material for the next MOSFET era. Using an ab-initio quantum-transport approach, the performance of n-type sub-5 nm devices built from a monolayer (ML) 2D AlN was studied. We found the double-gated (DG) ML AlN MOSFETs with an appropriate underlap region (UL) can meet the International Technology Roadmap for Semiconductors (ITRS) targets for 2028. Remarkably, the high-performance (HP) and low-power (LP) ML AlN MOSFET devices have high on-state currents (Ion) and perform up until gate lengths (Lg) of 2 and 3 nm, respectively. Additionally, ML AlN MOSFETs are competitive when compared to other 2D MOSFETs in terms of Ion, subthreshold swing (SS), delay time (†), and Power dissipation (PDP). Therefore, Moore's law could be stretched to a sub-5 nm range with AlN channel materials. [ABSTRACT FROM AUTHOR]

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Wafaa H Hussein,1 Hala Ramadan,2 Safa Labib,2 Gehan A Hegazy,3,4 Olfat G Shaker,5 Sherif M Yusuf,6 Mohammed A Hassanien,7 Maysa M Haroon1 1Rheumatology Department, Faculty of Medicine, Cairo University, Cairo, Egypt; 2Internal Medicine Department, Faculty of Medicine, Cairo University, Cairo, Egypt; 3Clinical Biochemistry Department, Faculty of Medicine, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia; 4Medical Division Department, National Research Centre, Giza, Egypt; 5Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Cairo, Egypt; 6Internal Medicine Department, Medical Research Institute, Alexandria University, Alexandria, Egypt; 7Pharmacy Practice Department, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Kingdom of Saudi ArabiaCorrespondence: Maysa M Haroon, Rheumatology Department, Faculty of Medicine, Cairo University, 71 El Kasr El Aini Street, P.O. Box 11562, Cairo, Egypt, Tel +201025868370, Email maysaharoon@yahoo.comBackground: Long noncoding ribonucleic acids (lncRNAs), small noncoding RNAs known as microRNAs (miRNAs) as well as some cytokines are recently thought to have a role in many inflammatory and autoimmune disorders including Behçet’s disease (BD). This chronic multisystem disease lacks the particular histological or laboratory findings that might aid in its diagnosis. Therefore, any association with such molecules may have an impact on understanding the disease pathogenesis and/or management. The current study compared the levels of NEAT1, miR-21 and IL17 levels in sera of Egyptian BD patients and healthy individuals. The expression levels of these molecules were further investigated for their association with BD manifestations and activity aiming to explore their potential application in disease management.Results: NEAT1 & miR-21 showed down-regulation while IL-17 showed up-regulation among BD patients as compared to controls. IL-17 had significant correlation with major vessels involvement and cyclophosphamide intake. NEAT1 showed a significant negative correlation with colchicine intake. Disease activity did not correlate significantly with any of NEAT1, miR-21 or IL-17.Conclusion: NEAT1, miR-21 and IL17 might have a role in Behçet’s disease pathogenesis, so more research is needed to unveil that role and their potential usage as biomarkers for the diagnosis or therapeutic targets.Keywords: long noncoding ribonucleic acids, microRNAs, NEAT1, miR-21, IL-17, Behçet’s disease

Abstract The environmental impact of trace metal contamination is a concern for Lake Small Abaya, Ethiopia, because of nearby agricultural and industrial activities. This study assessed the levels of trace metals (Pb, Zn, Cd, Cu, Fe, Mn, and Cr) in water, sediment, and fish samples, as well as the physicochemical parameters of the water. Samples were collected from five sites and analyzed via graphite furnace atomic absorption spectrometry (GFAAS). The mean concentrations in the water were Zn: 0.6971 mg/l, Fe: 0.5628 mg/l, Cu: 0.1168 mg/l, Mn: 0.2129 mg/l, Pb: 0.0057 mg/l, Cr: 0.0021 mg/l, and Cd: 0.0014 mg/l. In the sediments, the mean concentrations were as follows: Fe, 33.066 mg/kg; Zn, 18.263 mg/kg; Mn, 22.777 mg/kg; Cu, 7.5663 mg/kg; Pb, 0.0751 mg/kg; Cr, 0.0233 mg/kg; and Cd, 0.0183 mg/kg. The fish samples presented mean concentrations of Fe: 21.855 mg/kg, Zn: 12.686 mg/kg, Cu: 0.3185 mg/kg, Mn: 0.9181 mg/kg, Pb: 0.0089 mg/kg, Cd: 0.0075 mg/kg, and Cr: 0.0015 mg/kg. All the metal concentrations were generally below the World Health Organization (WHO) regulatory limits. The physicochemical parameters indicated slightly alkaline to neutral conditions, with electrical conductivity and total dissolved solids within acceptable ranges. These findings suggest that current agricultural and industrial activities do not significantly contribute to trace metal pollution. However, continued monitoring and pollution control measures are recommended to ensure the long-term health and sustainability of the lake. This study highlights the importance of effective environmental management strategies to safeguard this crucial aquatic resource.

Student-supervisor consultation is an essential academic requirement for Arab EFL male and female students to pass their postgraduate degree in Malaysian universities. During these consultations, students negotiate with their supervisors about their theses or final projects. However, these consultations form a challenge to these students as they need to demonstrate appropriate linguistic knowledge while corresponding to their supervisors' comments and corrections. One aspect of the linguistic challenge is the use of boosters as any over or misuse of these devices can be interpreted as inappropriate behavior, thus might affect the student-supervisor relationships and cause communication breakdown. The purpose of this study was to determine the types, frequencies, and functions of boosters in relation to gender as used by these students during the academic consultations. The data were collected by means of eight naturally occurring consultations and a pragmatic knowledge questionnaire and then analyzed quantitatively and qualitatively. The findings showed that the students used a variety of boosting devices with the intensifying adverbs, style adjuncts, and factive verbs being the most frequent. The gender analysis revealed differences in the types and counts of boosters with the male students generally using significantly higher frequencies of boosters than the female students. The pragmatic analysis showed that the Arab students used boosters strategically to reinforce their speech, so that they show their knowledge and appear more convincing. Further, the use of the boosters was not meant by the students to express polite or impolite behaviors, but it was mainly an appropriate politic behavior. [ABSTRACT FROM AUTHOR]

This study aimed to assess the reliability and validity of the Arabic version of the Glasgow Sleep Effort Scale (GSES) as an indicator of sleep effort within the Lebanese general population. A total of 360 members of the general population were included in this e-survey study. The sample completed the following measures: GSES, Insomnia Severity Index (ISI), Dysfunctional Beliefs about Sleep-2 (DBS-2), Patient Health Questionnaire-9 (PHQ-9), and a sleep diary regarding their bedtime, sleep onset time, wake-up time, desired total sleep time, and desired time in bed. The results supported the single-factor structure through confirmatory factor analysis. It also showed internal consistency with Cronbach’s alpha and omega values of 0.857 and 0.858, respectively. Further, Convergent validity was established with significant correlations between GSES, DBS-2, ISI, and PHQ-9. This suggests that GSES is valid and reliable for measuring sleep effort among the Lebanese general population.

The use of pragmatic markers as an aspect of language competence is necessary to present ideas and facts coherently. These markers mainly modify talk so that talk is comprehensible and meaningful, and help the audience follow the sequence of ideas. Failing to use these markers can negatively affect the audience's comprehension of the presentation and consequently affect the student's academic achievement. To this end, this study investigated the pragmatic markers used by Arab students during classroom oral presentations. The study focused on identifying the categories and subcategories of markers as well as examining their linguistic meaning and pragmatic functions. The data were collected using audio-recordings of students' oral presentations and were analyzed based on Fraser's (1996) classification and functions of pragmatic markers. The findings can inform better oral presentation performance of ESL/EFL learners in general and postgraduate students in particular. They add up to the literature of pragmatic discourses. [ABSTRACT FROM AUTHOR]

This research aims to produce lightweight self-compacting concrete (LWSCC) using lightweight expanded clay aggregate (LECA) as coarse aggregate. The additional aim is to study the influence of steel fiber and hybrid fibers (steel and polypropylene (PP)) on the properties of LWSCC in fresh and hardened state. Furthermore, compressive, tensile, and flexural strengths of LWSCC specimens (with and without fibers) are tested after being subjected to the fire exposure. In this study, four LWSCC mixtures with different fiber percentages (0 % fiber, 1 % steel, 0.75 % steel + 0.25 % PP, and 0.5 % steel + 0.5 % PP) are prepared and tested. The specimens were burned at temperatures 25, 300, 400, and 600 °C. The results show that all mixtures have excellent resistance to segregation and high ability to filling and passing. The presence of fibers slightly reduced the workability of LWSCC. The mechanical properties of LWSSC decrease with increasing temperature. The results show that mixtures containing fibers have good mechanical qualities and spalling resistance compared to mixtures without fibers when exposed to fire.

In this paper, the chaos synchronization of four Nano quantum cascade lasers with optoelectronic feedback was investigated. The present system consists of two receiver lasers and two transmitters lasers. The rate equation model was modulated to study the effect of the Purcell factor F and the spontaneous emission factor because of their importance in a nanocavity. The results indicated that the suggested system, realize the chaos synchronization. Also, the nanocavity parameters F and β, have significant effect on the chaos synchronization quality and may lead to lose it. Furthermore, the results showed the weak effect of delay time on the performance of lasers.

OBJECTIVES Coronavirus disease 2019 (COVID-19) has profoundly impacted residency and fellowship training and education. However, how and to what extent the COVID-19 pandemic in Lebanon compromised the daily involvement of trainees on the clinical and ethical levels is currently unknown, which this study will shed light on. METHODS We conducted a cross-sectional survey (30 questions) targeting medical residents delivering healthcare services in Lebanon. Residents from different specialties were included in the study to assess the effect of the pandemic on their education and the ethical obstacles they faced when dealing with patients. RESULTS A total of 221 postgraduate medical students participated in our study. Results showed that about half of the residents (52.1%) were only able to do a basic physical examination rather than a full examination as a mandatory requirement in the residency curriculum. The majority (60%) agreed that the doctor–patient relationship is contravened. In addition, almost all residents suffered from fear and emotional distress that affected their education (83.7%). CONCLUSION The findings of this study identify the effect of COVID-19 on residents’ training, which affects treatment outcomes and greatly impacts the mental well-being of both healthcare workers and patients.

Background: Odontogenic sinusitis is an underdiagnosed entity and is one cause of failure of conventional treatments of sinusitis. Unfortunately, there is no consensus so far on the best management protocol. This retrospective study aimed to suggest a practical management protocol that can reduce misdiagnosis and improve treatment outcomes. Methods: The study included 74 patients with confirmed odontogenic sinusitis who were diagnosed and treated over 10 years (2010–2019). The patient data were recorded and analysed. Results: Dental pain was reported in only 31.1 per cent of patients. Fifty-six patients (75.7 per cent) had received dental treatment during the last year, but only 13 (23.1 per cent) reported it. Dental pathology was missed on initial computed tomography evaluation in 24 patients (32.4 per cent). Forty-one patients (55.4 per cent) were successfully treated by dental procedures and antibiotics. Fourteen patients needed functional endoscopic sinus surgery in addition to dental procedures. Conclusion: Successful management of odontogenic sinusitis requires good communication between rhinologists, radiologists and dentists. Dental treatment should be the logical first step in the treatment protocol, unless otherwise indicated. [ABSTRACT FROM AUTHOR]

Maysa M Haroon,1 Gehan A Hegazy,2,3 Mohammed A Hassanien,4,5 Olfat Shaker,6 Wafaa H Hussein1 1Department of Rheumatology, Faculty of Medicine, Cairo University, Cairo, Egypt; 2Clinical Biochemistry Department, Faculty of Medicine, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia; 3Medical Division, National Research Centre, Giza, Egypt; 4Vice Presidency for Educational Affairs and Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia; 5Medical Biochemistry Department, Faculty of Medicine, Tanta University, Tanta, Egypt; 6Departments of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, EgyptCorrespondence: Maysa M Haroon, Department of Rheumatology, Faculty of Medicine, Cairo University, 71 El Kasr El Aini Street, P.O.Box 11562, Cairo, Egypt, Tel +201025868370, Email maysaharoon@yahoo.comBackground: Dysregulation of both cellular and humoral immune responses is central in systemic lupus erythematosus (SLE) pathogenetic mechanisms. Proinflammatory cytokines, such as interleukin 23 (IL23), and their roles in promoting such dysregulation have recently been highly considered. This research compared IL23 serum levels in 85 Egyptian SLE patients and 85 healthy controls. Then, IL23 level was correlated to various SLE disease parameters, disease activity, and damage indices.Results: IL23 serum levels were significantly elevated in SLE patients versus healthy individuals. Furthermore, IL23 levels were positively correlated with SLE disease activity index (SLEDAI) and were positively correlated with arthritis, seizures, consumption of complements (C3, C4), and with parameters of nephritis (hematuria, pyuria, casts, and proteinuria). A positive correlation was also found between IL23 levels and oral prednisolone dose.Conclusion: IL23 has higher levels in the serum of SLE patients, and is correlated to activity of the disease, especially lupus nephritis. Further researchis needed to explore its exact role in SLE pathogenesis and whether it can be considered a potential biomarker or therapeutic target in SLE.Keywords: interleukin 23, proinflammatory cytokines, systemic lupus erythematosus, SLE disease activity index, lupus nephritis

Background Acute kidney injury (AKI) complicates most cardiac surgery patients. This study assessed the accuracy of urine neutrophil gelatinase-associated lipocalin (uNGAL) and Renal Resistive Index (RRI) to early predict (AKI) after cardiopulmonary bypass (CPB).Methods This work enrolled 57 cases of elective cardiac surgery with CPB. Urinary NGAL and RRI were measured preoperatively and at 1, 6 and 24 h postoperatively. The primary outcome was AKI, defined by Acute Kidney Injury Network Classification (AKIN) System. The secondary aim was to assess the diagnostic accuracy, cut-off point of uNGAL and RRI, and their relation with AKI severity.Results AKI developed in 22 patients (38.5%), with 2 cases required dialysis. Mean uNGAL significantly increased by 10 folds at 1h and 15 folds at 6h postoperatively. As regard uNGAL,the 1 h diagnostic accuracy was 94.8%, The area under the curve (AUC) was 0.888, using a cut-off value of 194.3 ng/ml. The 6 h diagnostic accuracy was 96.5%, AUC was 0.995 at a cut-off value 67.1 ng/ml. The 1 and 6 h uNGAL were unrelated to AKI severity. As regard RRI, AKI patients had significantly higher 1 h and 6 h RRI. The diagnostic accuracy at 1 h was 93.0%, AUC was 0.991, using a cut-off value of 0.72. The diagnostic accuracy at 6 h was 94.8%, AUC 0.995 at a cut-off value of 0.71. Values of RRI were significantly related only to severe cases requiring dialysis.Conclusion Urinary NGAL and RRI are early accurate indicators of AKI. High RRI can predict dialysis-requirement.

Jiahui Zhang,1,* Jingyi Fan,1,* Mariusz Skwarczynski,1 Rachel J Stephenson,1 Istvan Toth,1– 3 Waleed M Hussein1 1School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, QLD, Australia; 2School of Pharmacy, The University of Queensland, Woolloongabba, QLD, Australia; 3Institute for Molecular Biosciences, The University of Queensland, St Lucia, QLD, Australia*These authors contributed equally to this workCorrespondence: Waleed M Hussein, School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, QLD, 4072, Australia, Tel +61 7 3365 2782, Email w.hussein@uq.edu.auAbstract: Persistent infection with high-risk human papillomaviruses (HPVs), such as HPV-16 and HPV-18, can induce cervical cancer in humans. The disease carries high morbidity and mortality among females worldwide. Inoculation with prophylactic HPV vaccines, such as Gardasil® or Cervarix®, is the predominant method of preventing cervical cancer in females 6 to 26 years of age. However, despite the availability of commercial prophylactic HPV vaccines, no therapeutic HPV vaccines to eliminate existing HPV infections have been approved. Peptide-based vaccines, which form one of the most potent vaccine platforms, have been broadly investigated to overcome this shortcoming. Peptide-based vaccines are especially effective in inducing cellular immune responses and eradicating tumor cells when combined with nanoscale adjuvant particles and delivery systems. This review summarizes progress in the development of peptide-based nanovaccines against HPV infection.Keywords: human papillomavirus, cervical cancer, peptide-based vaccines, nanovaccine

Background and Objective Our aim is to investigate the impact of COVID-19 on screen time among Lebanese high school students (grades 9–12). Methods An anonymous online questionnaire was distributed among 510 school students from different governorates in Lebanon; this included questions regarding screen time, food habits, and physical activity. Psychological symptoms were assessed using the Generalized Anxiety Disorder-7 items and Patient Health Questionnaire-9 items. Effects of screen time on sleep was evaluated using the Insomnia Severity Index and Bedtime Procrastination Scale. Results Female students reported higher depression (p = 0.018) and anxiety (p = 0.023) than male students; however, there was no difference in their sleep. Insomnia, depression, and anxiety were highest among phone users. A screen time of more than 7 hours per day was significantly associated with higher depression (67.9%), anxiety (61.6%), insomnia (82.1%), and bedtime procrastination. It also indicated a shift toward a less healthy diet and light exercise. Conclusions Seven hours of screen was found to develop depression and anxiety, exercising less, eating a less healthy diet, and experiencing disturbed sleep among school adolescents.

Introduction: Self-efficacy signifies an individual's belief in their own ability to perform the actions required to achieve a particular performance. In this study, we used an online survey to assess the mediation effect of resilience and self-efficacy on the overall psychological well-being of Lebanese people during the crises of the COVID-19 pandemic and the Beirut explosion.Methods: Overall, 567 Lebanese people participated in an online survey between March 17–28, 2021. The survey included the Stress and Anxiety to Viral Epidemics-6 items (SAVE-6), Patient Health Questionnaire-9 (PHQ-9), Connor–Davidson Resilience Scale-2 items, WHO-5 Well-being Index, General Self-Efficacy scale, and a single item on insomnia. We also assessed their risk perception regarding exposure to COVID-19 or explosions.Results: About 53% of participants were assessed as having depression (PHQ-9 ≥ 10) in the recent crisis. About half of participants (53.2%) reported feeling more stressed by COVID-19 than by the Beirut explosion, and 23.4% felt more stressed by the Beirut explosion than by COVID-19. Only the SAVE-6 score differed significantly between groups with greater stress responses to COVID-19 and the Beirut explosion. Self-efficacy mediated the influence of depression on people's psychological well-being, and self-efficacy and resilience mediated the influence of viral anxiety on psychological well-being.Conclusion: Self-efficacy is important for reducing people's depression and improving their psychological well-being during the Lebanon crises and also mediates the influence of anxiety in response to the viral epidemic on their psychological well-being in some people.

The root-knot nematode (RKN), Meloidogyne incognita, poses significant challenges for many important crops. Identified distinctive bacterial proteases have emerged as potential nematicides. In this study, the nprE from Bacillus subtilis 168 encoded the neutral metalloprotease, effectively enhancing it through a site-directed mutation. The enzyme-specific activity of pro118, secreted by the modified strain BS118, reached 215.4 U mg-1. It was twice that of pro168 produced by the wild-type strain BS168, which recorded an enzyme-specific activity of 101.6 U mg-1. The protease, pro118, caused the highest mortality in M. incognita juveniles, J2S (91.3% to 84.5%) after 48 h of in vitro treatment. Furthermore, when simultaneously applying pro118 with the nematode infection, it notably decreased the number of J2S, root galls, and egg masses/root systems by 19.5%, 18.8%, and 26.2%, respectively, compared with its application one week after the nematode infection. This consistent trend also manifested in the plant shoot length and fresh weight enrichment, which showed an increase of 9.7% and 14.7% in the first treatment versus the second. In conclusion, the study asserts that applying neutral metalloprotease as a bioagent to biocontrol M. incognita is a promising approach for mitigating the impact of this agricultural pest. [ABSTRACT FROM AUTHOR]

This study explores the phytochemical composition and biological activities of Verbascum yemenense, a plant known for its medicinal properties. The plant extract revealed a rich presence of bioactive compounds that exhibited significant antioxidant properties against free radicals. The enzyme inhibition potential was particularly notable against cholinesterases (AChE: 2.56 mg GALAE/g; BChE: 1.98 mg GALAE/g), and tyrosinase (87.94 mg KAE/g), α-glucosidase suggesting potential therapeutic applications in neurodegenerative diseases, skin disorders and diabetes. Molecular docking studies and Molecular Dynamics simulations, providing insights into the interaction mechanisms of the identified compounds with the target proteins. Molecular docking studies revealed high binding affinities of the phytoconstituents, with compounds like VY4 and phyllanthusol-A (VY15) showing substantial docking scores against AChE (-9.840 kcal/mol) and BChE (-9.853 kcal/mol), respectively. For instance, the RMSD values during the MD simulations for compound VY17 in the AML complex showed a stable conformation, fluctuating within a range of 0.75 Å to 1.75 Å, indicating a strong and consistent interaction with the enzyme. MESP studies highlighted VY17's distinctive electrostatic features, notably a pronounced electronegative region, which might contribute to its binding efficiency. These findings suggest that V. yemenense is a promising candidate for developing novel therapeutic agents., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Liver 3D cell models are regularly employed as a screening platform for predicting the metabolic safety of drugs, by monitoring the physiological responses of the spheroids, through the measurement of relevant markers of normal liver physiology, notably glucose. Measuring glucose levels within the spheroids and their surroundings provides insight into the metabolic homeostasis of liver cells and may be employed as an indication of potential drug-induced toxicity. Several ortho-aminomethyl phenylboronic acid (PDBA) glucose sensors have been developed. Most recently, Mc-CDBA ((((((2-(methoxycarbonyl)anthracene-9,10-diyl)bis(methylene)) bis(methylazanediyl))bis(methylene))bis(4-cyano-2,1-phenylene))diboronic acid) was reported. Although Mc-CDBA exhibits good water solubility and sensitivity toward glucose, its ability for intra- and extracellular glucose monitoring in spheroids has not been determined. Here, we present a set of Mc-CDBA derivatives: carboxylic (BA) and amide (BA 5)-based Mc-CDBA sensors for extra- and intracellular glucose monitoring, respectively. Both sensors exhibit superior spectroscopic features. BA 5 showed selective intracellular accumulation in liver spheroids and exhibited more than 3-fold higher basal fluorescence sensitivity compared to Mc-CDBA. These observations led to the development of an extracellular hydrogel-embedded sensor (HG-BA 21) to monitor extracellular glucose levels under persistent solution flow mimicking physiological conditions. We have therefore demonstrated that the sensors developed by our team are suitable for a variety of assays, notably with liver spheroids, and provide powerful new tools for organ-on-a-chip applications predicting drug-induced liver injury in the early stages of drug development., Competing Interests: The authors declare no competing financial interest., (© 2024 The Authors. Published by American Chemical Society.)

Outbreaks of furunculosis cause significant losses in salmonid aquaculture worldwide. With a recent rise in antimicrobial resistance, regulatory measures to minimize the use of antibiotics in animal husbandry, including aquaculture, have increased scrutiny and availability of veterinary medical products to control this disease in production facilities. In such a regulatory environment, the utility of autogenous vaccines to assist with disease prevention and control as a veterinary-guided prophylactic measure is of high interest to the producers and veterinary services alike. However, evolving concepts of epidemiological units and epidemiological links need to be considered during approval and acceptance procedures for the application of autogenous vaccines in multiple aquaculture facilities. Here, we present the results of solid-state nanopore sequencing (Oxford Nanopore Technologies, ONT) performed on 54 isolates of Aeromonas salmonicida ssp. salmonicida sampled during clinical outbreaks of furunculosis in different aquaculture facilities from Bavaria, Germany, from 2017 to 2020. All of the performed analyses (phylogeny, single nucleotide polymorphism and 3D protein modeling for major immunogenic proteins) support a high probability that all studied isolates belong to the same epidemiological unit. Simultaneously, we describe a cost/effective method of whole genome analysis with the usage of ONT as a viable strategy to study outbreaks of other pathogens in the field of aquatic veterinary medicine for the purpose of developing the best autogenous vaccine candidates applicable to multiple aquaculture establishments.

Leishmaniasis, a broad range of parasitic diseases, caused by Leishmania which is a flagellated intracellular protozoan parasite of the family Trypanosomatidae. The severity of leishmaniasis diseases ranges from minor cutaneous lesions to severe visceral illnesses that can be disfiguring and life-threatening. Cytokines are glycoprotein molecules produced by various cells in response to various immunological triggers. They regulate the body's innate and adaptive immunological responses. The aim of this study was to clarify the association of serum level and polymorphisms of IFN-γ with susceptibility to cutaneous leishmaniasis (CL). The whole blood 200 samples were collected from patients and controls from Diyala Governorate/ Iraq from October 2022 to February 2023 which were used to measure IFN-γ polymorphisms using High Resolution Melting technique. Enzyme-linked immunosorbent assay was used to detect the serum level of IFN-γ. The findings of this investigation showed that the IFN-γ serum concentration elevated significantly in patients compared to controls (P < 0.01). Also, the study found that the highest mean level IFN-γ concentrations were found in adults aged 46-55 years old for patients compared with controls with significant differences (P < 0.01). While, no significant differences were observed in the rest age groups except children aged 5-15 years old. Additionally, significant differences between patients and controls were revealed by polymorphisms data in all genetic models for genotypes GA, AA, (GA + AA) and allele A with (P < 0.01) and OR > 1. However, the distribution of IFN-γ serum levels by SNP (rs2069705) demonstrated no differences between genotypes except GG genotype which has significant differences for patients comparing to the same genotype in controls. Taking together, the SNP for IFN-γ (rs2069705) could be a risk factor for susceptibility infection with CL. Also, considered the mutant allele A as a risk allele and genotype AA in codominant genetic model as more risk factor than the genotype GA., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Angiotensin receptor blockers (ARBs) are commonly used to treat hypertension that target the hormonal system (renin-angiotensin system (RAS)), which regulates various physiological functions in the body. ARBs work by blocking the binding of angiotensin II to its receptor, thereby preventing a rise in blood pressure. These drugs not only normalize the overactivation of RAS but also provide protective effects against cardiovascular, renal, and type 2 diabetic patients. Inappropriate RAS activity has been linked to insulin resistance of type 2 diabetes. Olmesartan, as an ARB, was found to have a beneficial role in reducing postprandial glucose levels in type 2 diabetes. However, ARBs can cause side effects, prompting a search for new compounds that have fewer adverse effects. This study explores the potential of natural metabolites, specifically eugenol, gallic acid, myricetin, p -cymene, quercetin, and kaempferol, as ARB inhibitors compared to the current standard, olmesartan. Using in silico studies, the binding affinity of these natural substances to the ARB receptor was evaluated. The results showed that myricetin and kaempferol had affinities higher than those of olmesartan, suggesting that they could serve as promising ARB inhibitors for hypertension treatment. These natural compounds could provide an alternative approach to conventional antihypertensive drugs, which may have fewer side effects. However, more research is needed to validate the efficacy and safety of these natural compounds as antihypertensive drugs. Further in vitro and in vivo studies are needed to confirm their effectiveness and safety. This study provides a promising starting point for future investigations into the potential of natural metabolites as alternative treatments for hypertension. The findings also highlight the importance of exploring natural alternative treatments for hypertension and the protective effects of ARBs on early stage type-2 diabetics., Competing Interests: The authors declare no competing financial interest., (© 2024 The Authors. Published by American Chemical Society.)

Diabetic cardiomyopathy, a multifaceted complication of diabetes mellitus, remains a major challenge in clinical management due to its intricate pathophysiology. Emerging evidence underscores the pivotal role of autophagy dysregulation in the progression of diabetic cardiomyopathy, providing a novel avenue for therapeutic intervention. Noncoding RNAs (ncRNAs), a diverse class of regulatory molecules, have recently emerged as promising candidates for targeted therapeutic strategies. The exploration of various classes of ncRNAs, including microRNAs (miRNAs), long noncoding RNAs (lncRNAs), and circular RNAs (circRNAs) reveal their intricate regulatory networks in modulating autophagy and influencing the pathophysiological processes associated with diabetic cardiomyopathy. The nuanced understanding of the molecular mechanisms underlying ncRNA-mediated autophagic regulation offers a rationale for the development of precise and effective therapeutic interventions. Harnessing the regulatory potential of ncRNAs presents a promising frontier for the development of targeted and personalized therapeutic strategies, aiming to ameliorate the burden of diabetic cardiomyopathy in affected individuals. As research in this field advances, the identification and validation of specific ncRNA targets hold immense potential for the translation of these findings into clinically viable interventions, ultimately improving outcomes for patients with diabetic cardiomyopathy. This review encapsulates the current understanding of the intricate interplay between autophagy and diabetic cardiomyopathy, with a focus on the potential of ncRNAs as therapeutic targets., Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: There is no conflict of interest., (Copyright © 2024 Elsevier GmbH. All rights reserved.)

Janus kinase 2(JAK2) is a potential target for anticancer drugs in the treatment of numerous myeloproliferative diseases due to its central role in the JAK/STAT signaling cascade. In this study, the binding behavior of 2 amino-pyridine derivatives as JAK2 inhibitors was investigated by using multifaceted strategies including 3D-QSAR, molecular docking, Fingerprint analysis, MD simulations, and MM-PBSA calculations. A credible COMFA ( q 2 = 0.606 and r 2 = 0.919) and COMSIA ( q 2 = 0.641 and r 2 = 0.992) model was developed, where the internal and external validation revealed that the obtained 3D-QSAR models could be capable of predicting bioactivities of JAK2 inhibitors. The structural criteria provided by the contour maps of model were used to computationally develop more potent 100 new JAK2 inhibitors. Docking studies were conducted on the model data set and newly developed compounds (in-house library) to demonstrate their binding mechanism and highlight the key interacting residues within JAK2 active site. The selected docked complexes underwent MD simulation (100 ns), which contributed in the further study of the binding interactions. Binding free energy analyses (MMGB/PBSA) revealed that key residues such as Glu930, Leu932 (hinge region), Asp939 (solvent accessible region), Arg980, Asn981and Asp994 (catalytic site) have a significantly facilitate ligand-protein interactions through H-bonding and van der Waals interactions. The preliminary in-silico ADMET evaluation revealed encouraging results for all the modeled and in-house library compounds. The findings of this research have the potential to offer valuable recommendations for the advancement of novel, potent, and efficacious JAK2 inhibitors. Overall, this work has successfully employed a wide range of computer-based methodologies to understand the interaction dynamics between 2-amino-pyridine derivatives and the JAK2 enzyme, which is a crucial target in myeloproliferative disorders.Communicated by Ramaswamy H. Sarma.

The clinical spectrum of cutaneous leishmaniasis (CL), an intracellular parasitic pathogen, ranges from a single sore healing to chronic crusty lesions with a manifestation of treatment resistance. The complicated interaction between Leishmania bodies and the early immune response, including innate and adaptive mechanisms, determines the evolution of nodules. This study examined the levels of the chemoattractant interleukin 8 (IL-8), pro-inflammatory nitric oxide (NO), and immunoregulatory macrophage inhibitory factor (MIF) in the serum of subjects recently diagnosed with cutaneous leishmaniasis, in parallel with patients being monitored during consecutive sodium stibogluconate (Pentostam) treatment. A total of 161 serum samples of newly diagnosed individuals and patients undergoing pentostam injections were collected form an endemic area of Diyala, east central of Iraq. Sandwich ELISA was used to measure the level of IL-8, NO and MIF in the studied groups. Results of circulatory markers levels showed a considerable difference in all groups, with IL-8 being exceptionally higher in the first two groups of pretreated and dose-1 (191.5, 273.64) pg/ml respectively, while NO was found to be lower than in control subjects, particularly in the pretreated group (12.08 µmol/L) and MIF level was significantly higher in the pretreated group, which was (7.18 pg/ml). These findings can provide insights for distinction of disease phase and monitoring treatment efficacy along consecutive dosages, particularly in populations where CL is endemic., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Significance Statement: Dialysis-requiring AKI (AKI-D) now accounts for more than 15% of outpatient hemodialysis initiations; over 30% of these patients with AKI-D may have potential to recover. However, little is known about strategies currently used to treat outpatient AKI-D and screen for recovery. In this study of 1754 patients with AKI-D, we found that ( 1 ) the initial dialysis orders were similar to those of patients with contemporary incident ESKD, despite different treatment goals; ( 2 ) timed urine collections were completed in only a minority of patients; and ( 3 ) most patients with AKI-D who recovered discontinued dialysis without ever having been weaned from their initial dialysis prescription, suggesting there may be substantial opportunity to wean dialysis sooner., (Copyright © 2023 by the American Society of Nephrology.)

Ethnopharmacological Relevance: Artemisia sieberi Besser is a medicinal herb that has been traditionally used across the Middle East for the treatment of cancer. Further pharmacological studies on its extracts revealed that they possess cytotoxic activity against certain cancer cells, however, there were no studies conducted on the anticancer potential of Artemisia sieberi essential oil (ASEO)., Aim of the Study: To evaluate the anticancer potential of ASEO, elucidate the oil's mode of action for the first time and investigate its chemical composition., Materials and Methods: Artemisia sieberi was collected from Hail, Saudi Arabia, and its essential oil was obtained via hydrodistillation. The oil's activity against HCT116, HepG2, A549 and MCF-7 cells was assessed using SRB assay, while its anti-metastatic potential was assessed via a migration assay. Cell-cycle analysis and apoptosis assay were conducted via flow cytometry, while protein expression levels were investigated using Western blotting. The oil's chemical constituents were identified using GCMS., Results: ASEO exerted its highest cytotoxic activity against MCF-7 with an IC 50 value of 38.7 μg/ml. Further studies showed that the oil inhibited MCF-7 cells' migration, induced S-phase arrest and apoptosis. Western blot analysis showed no change in the expression level of caspase-3 after treatment, indicating the induction of caspase-independent apoptosis-like cell death in MCF-7. Treatment of MCF-7 with the oil resulted in downregulation of the protein expression levels of total ERK and its downstream target, LC3, indicating that any potential activation of the ERK signalling pathway during the cancer cells' growth would be inhibited. Finally, GCMS analysis identified the oil's major components as cis-crysanthenyl acetate (48.56%), davanone (10.28%), 1,8-cineole (6.81%) and caryophyllene diepoxide (5.34%), whereby it is suggested that these compounds might be responsible for the oil's bioactivity., Conclusion: ASEO possessed in vitro anticancer activity and modulated the ERK signalling pathway. This is the first study to explore the anticancer potential of ASEO in detail and reflects the significance of investigating essential oils from medicinal plants that have been traditionally used against cancer. This work might pave the way for further in vivo studies that could result in developing the oil into a natural effective anticancer treatment., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Digital technologies have recently become more advanced, allowing for the development of social networking sites and applications. Despite these advancements, phone calls and text messages still make up the largest proportion of mobile data usage. It is possible to study human communication behaviors and mobility patterns using the useful information that mobile phone data provide. Specifically, the digital traces left by the large number of mobile devices provide important information that facilitates a deeper understanding of human behavior and mobility configurations for researchers in various fields, such as criminology, urban sensing, transportation planning, and healthcare. Mobile phone data record significant spatiotemporal (i.e., geospatial and time-related data) and communication (i.e., call) information. These can be used to achieve different research objectives and form the basis of various practical applications, including human mobility models based on spatiotemporal interactions, real-time identification of criminal activities, inference of friendship interactions, and density distribution estimation. The present research primarily reviews studies that have employed mobile phone data to investigate, assess, and predict human communication and mobility patterns in the context of crime prevention. These investigations have sought, for example, to detect suspicious activities, identify criminal networks, and predict crime, as well as understand human communication and mobility patterns in urban sensing applications. To achieve this, a systematic literature review was conducted on crime research studies that were published between 2014 and 2022 and listed in eight electronic databases. In this review, we evaluated the most advanced methods and techniques used in recent criminology applications based on mobile phone data and the benefits of using this information to predict crime and detect suspected criminals. The results of this literature review contribute to improving the existing understanding of where and how populations live and socialize and how to classify individuals based on their mobility patterns. The results show extraordinary growth in studies that utilized mobile phone data to study human mobility and movement patterns compared to studies that used the data to infer communication behaviors. This observation can be attributed to privacy concerns related to acquiring call detail records (CDRs). Additionally, most of the studies used census and survey data for data validation. The results show that social network analysis tools and techniques have been widely employed to detect criminal networks and urban communities. In addition, correlation analysis has been used to investigate spatial-temporal patterns of crime, and ambient population measures have a significant impact on crime rates.

Background and Objectives: Ochradenus baccatus belongs to the family Resedaceae. It is widely spread in Saudi Arabia and other countries in Southwest Asia. O. baccatus is extensively used in traditional medicine as an anti-inflammatory and antibacterial agent, in addition to being a vital source of food for certain desert animal species. The aim of the present study was to investigate the chemical composition and antibacterial/anticancer activities of O. baccatus methanolic extracts collected from Hail, Saudi Arabia. Materials and Methods: The O. baccatus extracts were obtained by macerating the crude powder in methanol, followed by filtration and evaporation. Liquid chromatography-mass spectrometry ( LC-MS ) was used to analyze the methanolic extracts' chemical constituents. Broth microdilution assay for minimum inhibitory concentration (MIC) determination was used to assess antimicrobial activity, while the extracts' anticancer potential was assessed by sulforhodamine B Assay (SRB) assay. Results: The results of the antibacterial assay showed that the methanolic extracts from the roots and branches possessed varying degrees of activity against particular bacterial strains, with the highest activity being exerted by the branches' extract against Escherichia coli and Salmonella typhimurium (St), demonstrating MIC values of 15.6 µg/mL and 20 µg/mL, respectively. Furthermore, the SRB cell viability assay revealed that only the branches' extract inhibited the growth of A549 cancer cells, with an IC 50 value of 86.19 µg/mL. The LC-MS analysis of the methanolic extracts from the plant's roots and branches was then conducted, resulting in the identification of 8 and 13 major chemical constituents, respectively. Azelaic acid, β-amyrin, and phytanic acid are some of the bioactive compounds that were detected in the extracts via LC-MS , and they are thought to be responsible for the observed antibacterial/anticancer activity of O. baccatus methanolic extracts. Conclusions: This study confirmed the antibacterial/anticancer potential of O. baccatus methanolic extracts and analyzed their phytochemical constituents. Further isolation and biological screening are warranted to understand the therapeutic potential of O. baccatus .

Background: Metformin is a drug used to treat patients with type 2 diabetes, especially those who suffer from obesity. It is also used in the treatment of women with polycystic ovary syndrome (PCOS). This disease is related to insulin resistance and multiplied blood sugar ranges. Furthermore, it has been established that the use of metformin improves the menstrual cycles and ovulation rates of these women., Methods: A structured questionnaire was conducted to determine the prevalence of breast cancer among women using metformin in the Ha'il region., Result: The incidence of breast cancer among women using metformin in the Ha'il region is very low. Thus, it can be said that breast cancer cases declined among diabetics taking metformin. This means that metformin use is associated with a lower risk of breast cancer in women with type 2 diabetes, even in cases where these women have a family history of breast cancer., Conclusions: According to previous findings, metformin has been linked to lower breast cancer risk in women with type 2 diabetes. Furthermore, the findings of this study corroborate the literature on this subject by indicating that there is a substantial connection between metformin use and a lower risk of breast cancer in women with type 2 diabetes. However, further in vitro and in vivo experiments are crucial to investigate the protective effect of metformin against breast cancer and to confirm our findings., Competing Interests: The authors declare no conflicts of interest.

Background: Hepatitis C virus (HCV) is a global public health problem, with ~ 11 million people in Africa infected. There is incomplete information on HCV in Sudan, particularly in haemodialysis patients, who have a higher prevalence compared to the general population. Thus, our objectives were to genotype and molecularly characterize HCV isolated from end-stage renal disease haemodialysis patients., Methods: A total of 541 patients were recruited from eight haemodialysis centres in Khartoum and screened for anti-HCV. Viral loads were determined using in-house real-time PCR in seropositive patients. HCV was genotyped and subtyped using sequencing of amplicons of 5' untranslated (UTR) and non-structural protein 5B (NS5B) regions, followed by phylogenetic analysis of corresponding sequences., Results: The HCV seroprevalence in the study was 17% (93/541), with HCV RNA-positive viremic rate of 7% (40/541). A low HCV load, with a mean of 2.85 × 10 4  IU/ml and a range of 2.95 × 10 3 to 4.78 × 10 6  IU/ml, was detected. Phylogenetic analyses showed the presence of genotypes 1, 3, 4, and 5 with subtypes 1a, 1b, 1 g, 3a, 4a, 4 l, 4 m, 4 s, and 4t. Sequences of HCV from the same haemodialysis units, clustered in similar genotypes and subtypes intimating nosocomial infection., Conclusion: HCV infection is highly prevalent in haemodialysis patients from Sudan, with phylogenetic analysis intimating nosocomial infection. HCV genotyping is useful to locate potential transmission chains and to enable individualized treatment using highly effective direct-acting antivirals (DAAs)., (© 2022. The Author(s).)

Background: Diabetes-related vascular complications linked to increase in the expression of VEGF and its receptors. It helps to accelerate tissue damage inflicted by hyperglycemia, which is potential risk for diabetic complications. The study aimed to assess VEGF genetic polymorphism and its correlation with glucose and HbA1C level among Sudanese patients with diabetic retinopathy and nephropathy., Methods: A case-control study was conducted among a total of 252 subjects and divided into four groups of 63 subjects each. Glucose and HBA1c were measured then the VEGF gene was amplified using polymerase chain reaction. The data were analyzed using SPSS., Results: The HBA1c, and blood glucose levels had significantly (P value≤0.00001) highest mean in the DR group, DN group followed by DM. There is a non-significant correlation between VEGF Genotypes and HbA1c, and blood glucose levels (P value≤0.102, 0.173) Patients with GC genotypes will be 74.6%, and 54% higher at risk to develop DR, and DN respectively and 40 % lower at risk to develop DM than those without GC genotype. While patients with CC genotypes will be 22.2% higher at risk of developing DM and 9.5%, 12.2% higher at risk of developing DR and DN respectively., Discussion: The VEGF +405G/C gene polymorphism is linked to diabetic retinopathy, and diabetic nephropathy in type 2 Sudanese diabetics, and the presence of the GC genotypes and G allele is a significant predictor for retinopathy. There is no significant relation between HbA1C serum levels, blood glucose, and the VEGF +405G/C gene polymorphism., Competing Interests: All authors declare no conflict of interest.

Background: Caesarean section (CS) is a life-saving operation when vaginal delivery is risky to the mother or baby. However, if not medically indicated or performed under suboptimal conditions, it can be harmful and resource-intensive., Aims: To estimate the prevalence of CS in 6 Egyptian governorates and identify possible risk factors (including demographic, social and healthcare services factors) stratified according to geographical areas., Methods: We used secondary data collected from a large survey to investigate the sociodemographic and health indicators of 6 purposefully selected Egyptian governorates with suboptimal health indicators: 3 from Upper Egypt (Sohag, Assiut and Menia) and 3 from Lower Egypt (Sharkia, Beheira and Ismailia). The survey data were gathered using an interview questionnaire that targeted household members., Results: The CS rate was estimated at 55.1% for the 3 years preceding the study, and the highest rate was 67.8% in Behira and the lowest was 49.0% in Assiut. In most governorates, the CS rate was higher in rural than in urban areas, but the difference was not significant. High CS rates were significantly related to higher social class and lower number of children (≤ 3)., Conclusion: In the governorates investigated, CS was performed more often, with higher rates than those reported in the 2015 Egypt Health Issue Survey. The adoption of protocols for elective CS should be enforced with a more multisectoral effort. Pregnant women should be well informed of the risks associated with CS and its medical indications., (Copyright © World Health Organization (WHO) 2022. Open Access. Some rights reserved. This work is available under the CC BY-NC-SA 3.0 IGO license (https://creativecommons.org/licenses/by-nc-sa/3.0/igo).)

Introduction The management of proximal humeral fractures ranges greatly from conservative management to surgical treatment. For those fractures requiring surgical treatment, internal fixation is the primary method. The aim of internal fixation is to achieve rigid fracture fixation until union occurs, return of shoulder range of motion, and minimise intra-and postoperative complications. The aim of this study was to evaluate the results of the Proximal Humeral Interlocking System Plate (PHILOS) used for the treatment of three-and four-part proximal humeral fractures. Materials and methods This study included 30 patients with a mean age of 54 years (range 20-80 years). Results were checked post-operatively with standard radiographs and clinical evaluation according to the Constant-Murley shoulder score. All patients were followed up for 12 months. Results Union was achieved in all patients with a mean neck/shaft angle of 130° (range 108°-150°). The mean Constant-Murley score at the final follow-up was 82.28 (range 67-96) correlating with good results. No patients developed an intraoperative or postoperative vascular injury, wound complications, or avascular necrosis of the humeral head. Conclusion Our study has shown that the surgical treatment of three- and four-part proximal humeral fractures with the use of the PHILOS plate leads to a good functional outcome. It has also demonstrated the PHILOS plate and is an effective system for fracture stabilisation provided the correct surgical technique is used with awareness of potential hardware complications., Competing Interests: The authors have declared that no competing interests exist., (Copyright © 2022, Saber et al.)

Background: Several techniques were developed for managing hemorrhoidal disease, but their use in clinical practice and the general management of the condition seems highly variable in Saudi Arabia., Consensus Panel: To develop consensus recommendations that ensure the best possible diagnosis and treatment of hemorrhoidal disease in Saudi Arabia, the consensus panel consisted of experts in surgery in Saudi Arabia who met from December 2017 to September 2018., Consensus Findings: The discussions focused on the need: to set up a proctology society in Saudi Arabia to assess the prevalence of hemorrhoidal disease and to regulate the role of health-care professionals (HCPs) in the management of the disease; to initiate guidelines to ensure proper diagnosis (considering symptoms, medical history, and physical/clinical examination) and treatment (topical creams and suppositories should be limited as no strong evidence supports their efficacy); to educate patients on diet and lifestyle modifications using education materials and social media during and after the treatment (regular physical activity, drinking enough fluids, regular meal time with food rich in fibers, and regular bowel habit with non-straining defecation); to refer patients to a general/colorectal surgeon when needed; and to teach junior surgeons the best use of surgical techniques., Conclusion: These recommendations can be a step forward toward a recognized guidance for all HCPs in Saudi Arabia for a better management of hemorrhoidal disease. They will be of a great value for general practitioners, family medicine doctors, junior surgeons, and pharmacists who are the gate keepers and first contact with patients.

Aims: Paenibacillin is a naturally biosynthesized antimicrobial lantibiotic peptide which is produced by wild-type Paenibacillus polymyxa OSY-DF in low but detectable levels. The aim was to increase paenibacillin titre and production consistency through sequential drug resistance screening., Methods and Results: Spontaneous mutants of P. polymyxa OSY-DF were isolated by subjecting the bacterium to two rounds of screening for resistance to rifampicin, which targets RNA polymerase, and gentamicin, which targets ribosomes. Changes in antimicrobial production of the mutants were monitored using a bioassay method. A spontaneous mutant, P. polymyxa OSY-EC, capable of producing high paenibacillin titre, was selected and compared phenotypically to the wild-type strain. The mutant was found to produce paenibacillin at five-fold higher titre than the wild type. The mutant constantly produced paenibacillin while the wild type produced the antimicrobial agent variably. Fourier transformation mid-infrared spectroscopy revealed an interclass distance of 6·4 between the wild type and the mutant strain, suggesting significant phenotypic change during the mutation., Conclusions: P. polymyxa OSY-EC, a spontaneous mutant capable of consistent production of high paenibacillin titre, was isolated from the wild type after sequential screening on rationally selected antibiotics., Significance and Impact of the Study: The study will help make paenibacillin available for large-scale testing by interested researchers and industries seeking applications that improve food safety and quality., (© 2021 The Society for Applied Microbiology.)