Search

Your search keyword '"Huc, Laurence"' showing total 10 results
Start Over Author "Huc, Laurence" Publication Year Range Last 3 years
10 results on '"Huc, Laurence"'

4. Transcriptomic analysis in zebrafish larvae identifies iron-dependent mitochondrial dysfunction as a possible key event of NAFLD progression induced by benzo[a]pyrene/ethanol co-exposure

Author

Imran, Muhammad, Chalmel, Frédéric, Sergent, Odile, Evrard, Bertrand, Le Mentec, Hélène, Legrand, Antoine, Dupont, Aurélien, Bescher, Maëlle, Bucher, Simon, Fromenty, Bernard, Huc, Laurence, Sparfel, Lydie, Lagadic-Gossmann, Dominique, and Podechard, Normand

Published
2022
Full Text
View/download PDF

6. Data from c-Jun NH2-Terminal Kinase–Related Na+/H+ Exchanger Isoform 1 Activation Controls Hexokinase II Expression in Benzo(a)Pyrene-Induced Apoptosis

Author

Huc, Laurence, primary, Tekpli, Xavier, primary, Holme, Jørn A., primary, Rissel, Mary, primary, Solhaug, Anita, primary, Gardyn, Claire, primary, Le Moigne, Gwénaelle, primary, Gorria, Morgane, primary, Dimanche-Boitrel, Marie-Thérèse, primary, and Lagadic-Gossmann, Dominique, primary

Published
2023
Full Text
View/download PDF

7. Supplementary Methods from c-Jun NH2-Terminal Kinase–Related Na+/H+ Exchanger Isoform 1 Activation Controls Hexokinase II Expression in Benzo(a)Pyrene-Induced Apoptosis

Author

Huc, Laurence, primary, Tekpli, Xavier, primary, Holme, Jørn A., primary, Rissel, Mary, primary, Solhaug, Anita, primary, Gardyn, Claire, primary, Le Moigne, Gwénaelle, primary, Gorria, Morgane, primary, Dimanche-Boitrel, Marie-Thérèse, primary, and Lagadic-Gossmann, Dominique, primary

Published
2023
Full Text
View/download PDF

8. Supplementary Figures 1-3 from c-Jun NH2-Terminal Kinase–Related Na+/H+ Exchanger Isoform 1 Activation Controls Hexokinase II Expression in Benzo(a)Pyrene-Induced Apoptosis

Author

Huc, Laurence, primary, Tekpli, Xavier, primary, Holme, Jørn A., primary, Rissel, Mary, primary, Solhaug, Anita, primary, Gardyn, Claire, primary, Le Moigne, Gwénaelle, primary, Gorria, Morgane, primary, Dimanche-Boitrel, Marie-Thérèse, primary, and Lagadic-Gossmann, Dominique, primary

Published
2023
Full Text
View/download PDF

9. Characterization of human isogenic epithelial cell lines as a relevant tool to study colon carcinogenesis and interaction between genes and environment

Author

Tête, Arnaud, Arnaud, Liana C., Le Mentec, Hélène, Gallais, Isabelle, Poupin, Nathalie, Tournadre, Noémie, Duarte-Hospital, Carolina, Lippi, Yannick, Mathevet, Fanny, Lefort, Gaëlle, Burel, Agnes, Surya, R., Boutet-Robinet, Elisa, Shay, Jerry W., Vialaneix, Nathalie, Bortoli, Sylvie, Lagadic-Gossmann, Dominique, Huc, Laurence, Vialaneix, Nathalie, Toxicité environnementale, cibles thérapeutiques, signalisation cellulaire (T3S - UMR_S 1124), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), ToxAlim (ToxAlim), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Ecole d'Ingénieurs de Purpan (INP - PURPAN), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Contaminants & Stress Cellulaire (ToxAlim-COMICS), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), EHESP-Irset (EHESP-Irset), École des Hautes Études en Santé Publique [EHESP] (EHESP), Métabolisme et Xénobiotiques (ToxAlim-MeX), Université Paris Descartes, Sorbonne Paris Cité, Transcriptomic impact of Xenobiotics (E23 TRiX), Plateforme Génome & Transcriptome (GET), Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-ToxAlim (ToxAlim), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Ecole d'Ingénieurs de Purpan (INP - PURPAN), Unité de Mathématiques et Informatique Appliquées de Toulouse (MIAT INRAE), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Biosit : biologie, santé, innovation technologique (SFR UMS CNRS 3480 - INSERM 018), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Bina Nusantara University [Jakarta], University of Texas Southwestern Medical Center, and ITMO Cancer, METAhCOL

Subjects
[SDV.CAN] Life Sciences [q-bio]/Cancer, [SDV.CAN]Life Sciences [q-bio]/Cancer, digestive system diseases
Abstract

International audience; Colorectal cancer (CRC) is the fourth most common cause of death from cancer worldwide. CRC is a multistep and progressive disease where genetic factors are important in the initiation, the development and the progression of the disease. CRC can arise from sequential steps including the acquisition of mutations in the adenomatous polyposis coli (APC), followed by the mutational activation of oncogene KRAS and the inactivation of the tumor suppressor gene, TP53. The occurrence of colorectal cancer is largely influenced by the environment, including food contaminants, lifestyle and nutrition. However, the influence of mutations on the response to environmental pollutants is poorly evaluated. Environmental carcinogenesis lacks robust models to explore the interaction between genes and environment and to determine whether genetic mutations associated with colon carcinogenesis generate a particular susceptibility to the harmful effects of pollutants. Here, we outline the characterization of an innovative cellular model that consists in 6 human isogenic epithelial colon cell lines bearing mutations on driver genes of CRC. Altogether, these cell lines recapitulate colon carcinogenesis from the healthy, preneoplastic, adenoma and carcinoma stages in a simplified way. We aim to perform a phenotypic and metabolic description of these cell lines, with a particular focus on their ability to express the battery of detoxification enzymes, to have a differential sensitivity to genotoxicity and cell death stimuli, to display a flexible energy metabolism, to migrate and grow without anchorage. We show that these models of human isogenic colonic cells of CRC could be a powerful and relevant tool to study the effects of environmental pollutants on the colorectal carcinogenesis from the early to the late and metastatic stages and to evaluate gene-environment interactions in food toxicology.

Published
2022

10. Transcriptomic analysis in zebrafish larvae identifies iron-dependent mitochondrial dysfunction as a possible key event of NAFLD progression induced by benzo[a]pyrene/ethanol co-exposure.

Author

Imran, Muhammad, Chalmel, Frédéric, Sergent, Odile, Evrard, Bertrand, Le Mentec, Hélène, Legrand, Antoine, Dupont, Aurélien, Bescher, Maëlle, Bucher, Simon, Fromenty, Bernard, Huc, Laurence, Sparfel, Lydie, Lagadic-Gossmann, Dominique, and Podechard, Normand

Subjects
POLLUTANTS, NON-alcoholic fatty liver disease, IRON in the body, ARYL hydrocarbon receptors, MITOCHONDRIA, DIOXYGENASES
Abstract

Non-alcoholic fatty liver disease (NAFLD) is a worldwide epidemic for which environmental contaminants are increasingly recognized as important etiological factors. Among them, the combination of benzo[a]pyrene (B[a]P), a potent environmental carcinogen, with ethanol, was shown to induce the transition of steatosis toward steatohepatitis. However, the underlying mechanisms involved remain to be deciphered. In this context, we used high-fat diet fed zebrafish model, in which we previously observed progression of steatosis to a steatohepatitis-like state following a 7-day-co-exposure to 43 mM ethanol and 25 nM B[a]P. Transcriptomic analysis highlighted the potent role of mitochondrial dysfunction, alterations in heme and iron homeostasis, involvement of aryl hydrocarbon receptor (AhR) signaling, and oxidative stress. Most of these mRNA dysregulations were validated by RT-qPCR. Moreover, similar changes were observed using a human in vitro hepatocyte model, HepaRG cells. The mitochondria structural and functional alterations were confirmed by transmission electronic microscopy and Seahorse technology, respectively. Involvement of AhR signaling was evidenced by using in vivo an AhR antagonist, CH223191, and in vitro in AhR-knock-out HepaRG cells. Furthermore, as co-exposure was found to increase the levels of both heme and hemin, we investigated if mitochondrial iron could induce oxidative stress. We found that mitochondrial labile iron content was raised in toxicant-exposed larvae. This increase was prevented by the iron chelator, deferoxamine, which also inhibited liver co-exposure toxicity. Overall, these results suggest that the increase in mitochondrial iron content induced by B[a]P/ethanol co-exposure causes mitochondrial dysfunction that contributes to the pathological progression of NAFLD. [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results