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Viral diseases are among the main threats to public health. Understanding the factors affecting viral invasion is important for antiviral research. Until now, it was known that most viruses have very low plaque-forming unit (PFU)-to-particle ratios. However, further investigation is required to determine the underlying factors. Here, using quantitative single-particle analysis methods, the invasion of Semliki Forest virus (SFV), Japanese encephalitis virus (JEV), and influenza A virus (IAV) containing attachment to the cell surface, entry into the cell, transport towards the cell interior, and fusion with endosomes to release nucleocapsids were quantitatively analysed in parallel. It was found that for SFV with an PFU-to-particle ratio of approximately 1:2, an entry efficiency of approximately 31% limited infection. For JEV, whose PFU-to-particle ratio was approximately 1:310, an attachment efficiency of approximately 27% and an entry efficiency of 10% were the main factors limiting its infection. Meanwhile, for IAV with PFU-to-particle ratios of 1:8100, 5% attachment efficiency, 9% entry efficiency, and 53% fusion efficiency significantly limited its infection. These results suggest that viruses with different infectivities have different limited steps in the invasion process. Moreover, there are significant differences in attachment efficiencies among viruses, emphasizing the pivotal role of attachment in viral invasion. The influence of the virus purification method on virus invasion was also investigated. This study, for the first time, reports the efficiencies of different stages of virus invasion, leading to a better understanding of virus invasion and providing a protocol to quantitatively analyse the virus invasion efficiency.

Lateral intakes are common in rivers. The pump efficiency and sediment deposition are determined by the local hydrodynamic characteristics and mainstream division width. The hydraulic characteristics of lateral withdrawal from inclined river slopes at different intake elevations should be investigated. Meanwhile, the division width exhibits significant vertical non-uniformity at an inclined river slope, which should be clarified. Hence, a three-dimensional (3-D) hydrodynamic and particle-tracking model was developed with the Open Source Field Operation and Manipulation (OpenFOAM), and the model was validated with physical model tests for 90° lateral withdrawal from an inclined side bank. The flow fields, withdrawal sources, and division widths were investigated with different intake bottom elevations, withdrawal discharges, and main channel velocities. This study showed that under inclined side bank conditions, water entered the intake at an oblique angle, causing significant 3-D spiral flows in the intake rather than two-dimensional closed recirculation. A lower withdrawal discharge, a lower bottom elevation of the intake, or a higher main channel velocity could further strengthen this phenomenon. The average division width and turbulent kinetic energy were smaller under inclined side bank conditions than under vertical bank conditions. With a low intake bottom elevation, a low withdrawal discharge, or a high main channel velocity, the sources of lateral withdrawal were in similar ranges near the local inclined bank in the vertical direction. Under inclined slope conditions, sediment deposition near the intake entrance could be reduced, compared to that under vertical slope conditions. The results provide hydrodynamic and sediment references for engineering designs for natural rivers with inclined terrains.