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Your search keyword '"He, Bai‐Liang"' showing total 8 results
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8 results on '"He, Bai‐Liang"'

5. Follistatin Alleviates Hepatic Steatosis in NAFLD via the mTOR Dependent Pathway

Author

Tong,Junlu, Cong,Li, Jia,Yingbin, He,Bai-Liang, Guo,Yifan, He,Jianzhong, Li,Decheng, Zou,Baojia, Li,Jian, Tong,Junlu, Cong,Li, Jia,Yingbin, He,Bai-Liang, Guo,Yifan, He,Jianzhong, Li,Decheng, Zou,Baojia, and Li,Jian

Abstract

Junlu Tong1,2 *, Li Cong1 *, Yingbin Jia,3 Bai-Liang He,4 Yifan Guo,1 Jianzhong He,5 Decheng Li,3 Baojia Zou,3 Jian Li3 1Department of Endocrinology, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, People’s Republic of China; 2Department of Central Laboratory, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, People’s Republic of China; 3Department of Hepatobiliary Surgery, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, People’s Republic of China; 4Guangdong Provincial Key Laboratory of Biomedical Imaging, Guangdong Provincial Engineering Research Center of Molecular Imaging, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, People’s Republic of China; 5Department of Pathology, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jian Li; Baojia Zou, Department of Hepatobiliary Surgery, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, People’s Republic of China, Tel +86-756-252-8781, Email lijian5@mail.sysu.edu.cn; zoubj6@mail.sysu.edu.cnPurpose: In this study, we aimed to investigate the effect of follistatin (FST) on hepatic steatosis in NAFLD and the underlying mechanism, which has rarely been reported before.Methods: Liver samples from NAFLD patients and normal liver samples (from liver donors) were collected to investigate hepatic FST expression in humans. Additionally, human liver cells (LO2) were treated with free fatty acid (FFA) to induce lipid accumulation. Furthermore, lentivirus with FST overexpression or knockdown vectors were used to generate stable cell lines, which were subsequently treated with FFA or FFA and rapamycin. In the animal experiments, male C57BL/6J mice were fed with a high-fat diet (HFD) to induce NAFLD, after which the adeno-associated virus (AAV) gene vectors for FST overexpression were administered. In both cell culture

Published
2022

7. Transgenic IDH2R172Kand IDH2R140Qzebrafish models recapitulated features of human acute myeloid leukemia

Author

Wang, Dandan, Zheng, Lichuan, Cheng, Bowie Yik Ling, Sin, Chun-Fung, Li, Runsheng, Tsui, Sze Pui, Yi, Xinyu, Ma, Alvin Chun Hang, He, Bai Liang, Leung, Anskar Yu Hung, and Sun, Xuan

Abstract

Isocitrate dehydrogenase 2 (IDH2) mutations occur in more than 15% of cytogenetically normal acute myeloid leukemia (CN-AML) but comparative studies of their roles in leukemogenesis have been scarce. We generated zebrafish models of IDH2R172Kand IDH2R140QAML and reported their pathologic, functional and transcriptomic features and therapeutic responses to target therapies. Transgenic embryos co-expressing FLT3ITDand IDH2mutations showed accentuation of myelopoiesis. As these embryos were raised to adulthood, full-blown leukemia ensued with multi-lineage dysplasia, increase in myeloblasts and marrow cellularity and splenomegaly. The leukemia cells were transplantable into primary and secondary recipients and resulted in more aggressive disease. Tg(Runx1:FLT3ITDIDH2R172K) but not Tg(Runx1:FLT3ITDIDH2R140Q) zebrafish showed an increase in T-cell development at embryonic and adult stages. Single-cell transcriptomic analysis revealed increased myeloid skewing, differentiation blockade and enrichment of leukemia-associated gene signatures in both zebrafish models. Tg(Runx1:FLT3ITDIDH2R172K) but not Tg(Runx1:FLT3ITDIDH2R140Q) zebrafish showed an increase in interferon signals at the adult stage. Leukemic phenotypes in both zebrafish could be ameliorated by quizartinib and enasidenib. In conclusion, the zebrafish models of IDH2mutated AML recapitulated the morphologic, clinical, functional and transcriptomic characteristics of human diseases, and provided the prototype for developing zebrafish leukemia models of other genotypes that would become a platform for high throughput drug screening.

Published
2023
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