1. Candidate antibody reference reagents for Chlamydia trachomatis serology.
- Author
-
da Silva FC, Kamuyu G, Michels B, Edney J, Hassall L, Stickings P, Maharjan S, Waterboer T, and Beddows S
- Subjects
- Humans, Female, Adult, Reference Standards, Porins immunology, Young Adult, Serologic Tests standards, Serologic Tests methods, Bacterial Proteins immunology, Chlamydia trachomatis immunology, Antibodies, Bacterial blood, Antibodies, Bacterial immunology, Antigens, Bacterial immunology, Bacterial Outer Membrane Proteins immunology, Chlamydia Infections immunology, Chlamydia Infections diagnosis, Chlamydia Infections microbiology
- Abstract
Chlamydia trachomatis (Ct) serology is an important tool for monitoring infection and disease burden but there are currently no formal reference reagents to harmonize results reporting. Our objective was to develop a panel of candidate reference reagents with reactivity against the major outer membrane protein (MOMP) and virulence factor (pgp3) antigens. Plasma packs from females (20-40 years old) were screened against MOMP and pgp3 antigens and selected positive and negative samples pooled to create a panel of candidate antibody reference reagents that were tested in two laboratories. Antigen specificity and internal quality assurance were also evaluated. Suitable candidate materials have been selected to produce Ct reference reagents., Competing Interests: Declaration of competing interest None., (Crown Copyright © 2024. Published by Elsevier B.V. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF