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Several influential thought experiments from Harman 1973 purport to show that unpossessed evidence can undermine knowledge. Recently, some epistemologists have appealed to these thought experiments in defense of a logically stronger thesis: unpossessed evidence can defeat justification. But these appeals fail to appreciate that Harman himself thought of his examples as Gettier cases, and so would have rejected this strengthening of his thesis. On the contrary, he would have held that while unpossessed evidence can undermine knowledge, it leaves justification intact. In this paper I seek to undermine the viability of Harman's position. If this is correct, contemporary epistemology faces a choice: either we reject that unpossessed evidence in Harman-style cases bears on knowledge at all, or else we must allow that it undermines knowledge by defeating justification. The former option must explain why Harman's thought experiments elicit strong 'no knowledge' intuitions; the latter option embraces a minority view about the bearing of social expectations on the assessment of knowledge and justification (= the doctrine of normative defeat). [ABSTRACT FROM AUTHOR]

The main problem that speculative realism, represented by Quentin Meillassoux, Graham Harman and Ray Brassier, is trying to solve is overcoming correlationism. Two important figures who played a key role in the development of correlationism are Immanuel Kant and Martin Heidegger. Correlationism is a type of philosophizing that postulates that a person has access only to the connection (correlation) between the subject and the thing in itself, but not to these instances separately. The correlation is often anthropic in nature, i.e. it is language, social practices, culture, consciousness, history, meaning, and so on. Speculative realism seeks to overcome correlationism and show how one can approach the thing in itself. Heidegger played a significant role in the development of correlationism - speculative realists are in active dialogue with him. It is shown that the interpretation and criticism of Heidegger's ideas by speculative realists is untenable. Speculative realists are often even greater correlationists than Heidegger, since they replace the concept of the thing in itself with the thing for us. It is concluded that correlationism cannot be overcome, but it is necessary to reduce its influence through a number of techniques. Such practices include the restoration of the idea of the Kantian thing in itself. It is also necessary to introduce the idea of a passive subject who reduces his cognitive claims and switches attention to the affect of the thing in itself. In addition, we must remember that the human race is not the center of the world drama, and its fate depends on how carefully it learns to handle natural forces that exist independently of it in order to avoid climate catastrophe.

Graham Harman’s Object-Oriented Ontology (OOO) emphasizes the autonomy of objects, positing a withdrawn surplus of being that resists reduction to its parts or the sum of its parts. However, Harman’s framework faces conceptual tensions, including challenges in reconciling epistemological and ontological dimensions, explaining the formation of compound objects, and ascribing determinate features to experientially inaccessible objects. I argue that these issues arise mostly due to Harman’s over-commitment to a withdrawn substantial core of objects. To address these issues, I propose turning to Jean-Luc Nancy’s post-phenomenological materialism and Alain Badiou’s mathematical realism. Both Nancy and Badiou offer alternatives to Harman’s substantialist core, emphasizing a local, contextually bound identity of things, which describes a restricted domain from the endlessly broader horizon of relations and situations in which each thing is or can be involved. They thus account for the surplus of being in each thing and consequently their ontological autonomy without recourse to a substantive grounding. I argue such a relational-friendly account of pluralism avoids the conceptual issues Harman’s OOO runs into. Lastly, I emphasize the value of OOO as an advocate for ontological equality that seeks to avoid the emergence of privileged actors or centralized discourse. This dialogue between Harman, Nancy, and Badiou thus seeks to advance the possibility of pluralistic ontologies within relational frameworks, combining the perspectives of OOO with post-phenomenological theories to explore the complexities of relationality without recourse to non-relational substrata.

This paper attempts to contextualize a philosophy of curation that is object‐oriented or toward a "return to the object." In the museum, three interrelated philosophical problems pervade curation practices that prevent access to the object as it is. Here, the subject‐object relations or idealism‐realism issues are reconsidered as a specific niche of the philosophy of curation. To address these issues, this paper claims that Jean‐Paul Martinon and Graham Harman's philosophical return to the Heideggerian fourfold (das Geviert) can introduce creative pathways for the curated object that is riddled with excess and tensions. Later, with some caveats, the paper addresses the issues and suggests a possible avenue for further research. [ABSTRACT FROM AUTHOR]

This article seeks to clarify the role of the notion of rhythm in Whitehead's philosophy, considering the mediating character it has between the order of events and that of objects. Deleuze and Harman, when commenting on Whitehead's ontological system, have highlighted the value of events and objects, respectively. Here, we pretend to elucidate the limits of these readings and give relevance to the category between both planes, which is rhythm. This concepts evolve from a naturalistic view to a more metaphysical conception in Whitehead's later work, and its applications go from science to education and aesthetics. Thus, rhythm can be understood as a complex that has an eventual face and an objectual face, internally defining itself as a system of contrasts that mobilizes creation and intensity through time. This rhythmic process of creation leads us to think a poetics of experience, considering its aesthetic and compositional nature. [ABSTRACT FROM AUTHOR]

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Copyright of Contrastes: Revista Internacional de Filosofia is the property of Contrastes: Revista Internacional de Filosofia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

芝麻油可能含有杂环胺9H-吡啶并\[3,4-b\]吲哚（norharman）和1-甲基-9H-吡啶并\[3,4-b\]吲哚（harman），为了有效检测芝麻油中杂环胺含量，建立了一种高效液相色谱-荧光检测法（HPLC-FLD），并对不同产地芝麻所制芝麻油中杂环胺含量进行对比。结果表明：所建立的HPLC-FLD能在15 min内实现对芝麻油中杂环胺的测定；在相同炒籽条件下，不同产地芝麻所制芝麻油中杂环胺含量有显著差异（p

This paper explores the issues connected between rural sustainable development in formerly state-socialist countries and the local cultural heritage of rural areas. It pays specific attention to the potential of cultural tourism which can enhance local rural development. This paper is a case study of the Hărman commune, and this area is investigated in depth. It is one of the most important rural and cultural areas located in Brașov County of Romania, a country with an impressive cultural heritage concentrated in its rural areas. The study uses a mixed-method analysis combining quantitative and qualitative research (focus groups, interviews, oral histories, and personal conversions), participatory ethnographic observation, and logical framework analysis (LFA). The main findings of the study illustrate that the Hărman commune has an important cultural heritage which could be more capitalized on in the future through the lens of cultural tourism to ensure local sustainability and to open up new perspectives in terms of local development, connecting rural and cultural tourism with other economic activities. Furthermore, the main findings of this study represent, beyond an informative platform for the local actors in rural development, an inspiring instrument that could frame new policies in local rural sustainable development and fertile backgrounds for new debates in local rural sustainability, enriching local agendas on rural sustainable development through cultural heritage capitalization and cultural tourism. [ABSTRACT FROM AUTHOR]

β-carbolines (harman and norharman) are potentially mutagenic and have been reported in some vegetable oils. Sesame seed oil is obtained from roasted sesame seeds. During sesame oil processing, roasting is the key procedure to aroma enhancement, in which β-carbolines are produced. Pressed sesame seed oils cover most market share, while leaching solvents are used to extract oils from the pressed sesame cake to improve the utilization of the raw materials. β-carbolines are nonpolar heterocyclic aromatic amines with good solubility in leaching solvents (n-hexane); therefore, the β-carbolines in sesame cake migrated to the leaching sesame seed oil. The refining procedures are indispensable for leaching sesame seed oil, in which some small molecules can be reduced. Thus, the critical aim is to evaluate the changes in β-carboline content during the refining of leaching sesame seed oil and the key process steps for the removal of β-carbolines. In this work, the levels of β-carbolines (harman and norharman) in sesame seed oil during chemical refining processes (degumming, deacidification, bleaching and deodorization) have been determined using solid phase extraction and high performance liquid chromatography-mass spectrometry (LC-MS). The results indicated that in the entire refining process, the levels of total β-carbolines greatly decreased, and the adsorption decolorization was the most effective process in reducing β-carbolines, which might be related to the adsorbent used in the decolorization process. In addition, the effects of adsorbent type, adsorbent dosage and blended adsorbent on β-carbolines in sesame seed oil during the decolorization process were investigated. It was concluded that oil refining can not only improve the quality of sesame seed oil, but also reduce most of the harmful β-carbolines. [ABSTRACT FROM AUTHOR]

Monoamine oxidase (MAO) oxidizes neurotransmitters and xenobiotic amines, including vasopressor and neurotoxic amines such as the MPTP neurotoxin. Its inhibitors are useful as antidepressants and neuroprotectants. This work shows that diluted soy sauce (1/3) and soy sauce extracts inhibited human MAO-A and -B isozymes in vitro, which were measured with a chromatographic assay to avoid interferences, and it suggests the presence of MAO inhibitors. Chromatographic and spectrometric studies showed the occurrence of the β-carboline alkaloids harman and norharman in soy sauce extracts inhibiting MAO-A. Harman was isolated from soy sauce, and it was a potent and competitive inhibitor of MAO-A (0.4 µM, 44 % inhibition). The concentrations of harman and norharman were determined in commercial soy sauces, reaching 243 and 52 μg/L, respectively. Subsequently, the alkaloids 1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid (THCA) and 1-methyl-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid (MTCA) were identified and analyzed in soy sauces reaching concentrations of 69 and 448 mg/L, respectively. The results show that MTCA was a precursor of harman under oxidative and heating conditions, and soy sauces increased the amount of harman under those conditions. This work shows that soy sauce contains bioactive β-carbolines and constitutes a dietary source of MAO-A and -B inhibitors. [ABSTRACT FROM AUTHOR]

Copyright of Academic Journal of Agriculture / Akademik Ziraat Dergisi is the property of University of Ordu, Faculty of Agriculture, Academic Journal of Agriculture and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The prenylation of compounds has attracted much attention, since it often adds bioactivity to non-prenylated compounds. We employed an enzyme assay with CdpNPT, an indole prenyltransferase from Aspergillus fumigatus with two naturally occurring β-carbolines, harmine (3) and harman (4) as prenyl acceptors, in the presence of dimethylallyl diphosphate (DMAPP) as the prenyl donor. The enzyme accepted these two prenyl acceptor substrates to produce 6-(3′,3′-dimethylallyl)harmine (5) from 3 and 9-(3′,3′-dimethylallyl)harman (6) and 6-(3′,3′-dimethylallyl)harman (7) from 4. The X-ray crystal structure analysis of the CdpNPT (38–440) truncated mutant complexed with 4 , and docking simulation studies of DMAPP to the crystal structure of the CdpNPT (38–440) mutant, suggested that CdpNPT could employ the two-step prenylation mechanism to produce 7 , while the enzyme produced 6 with either one- or two-step prenylation mechanisms. Furthermore, the antibacterial assays revealed that the 3′,3′-dimethylallylation of 3 and 4 , as well as harmol (1), at C-6 enhanced the activities against Staphylococcus aureus and Bacillus subtilis. [Display omitted] • Prenylation ability of CdpNPT toward two β-carbolines was investigated. • CdpNPT accepted harmine to produce 6-(3-dimethylallyl)harmine. • CdpNPT also has the ability to produce 9- and 6-(3-dimethylallyl)harmans. • 3-Dimethylallylation of harmine and harman enhanced antibacterial activities. [ABSTRACT FROM AUTHOR]

This paper explores the issues connected between rural sustainable development in formerly state-socialist countries and the local cultural heritage of rural areas. It pays specific attention to the potential of cultural tourism which can enhance local rural development. This paper is a case study of the Hărman commune, and this area is investigated in depth. It is one of the most important rural and cultural areas located in Brașov County of Romania, a country with an impressive cultural heritage concentrated in its rural areas. The study uses a mixed-method analysis combining quantitative and qualitative research (focus groups, interviews, oral histories, and personal conversions), participatory ethnographic observation, and logical framework analysis (LFA). The main findings of the study illustrate that the Hărman commune has an important cultural heritage which could be more capitalized on in the future through the lens of cultural tourism to ensure local sustainability and to open up new perspectives in terms of local development, connecting rural and cultural tourism with other economic activities. Furthermore, the main findings of this study represent, beyond an informative platform for the local actors in rural development, an inspiring instrument that could frame new policies in local rural sustainable development and fertile backgrounds for new debates in local rural sustainability, enriching local agendas on rural sustainable development through cultural heritage capitalization and cultural tourism.

BackgroundHeterocyclic aromatic amines (HAAs) are a group of harmful substances produced while cooking meat at high temperatures. This study aimed to investigate the relationship between HAAs and the occurrence of kidney stones.MethodsData on the level of four HAAs, including 2-Amino-9H-pyrido [2, 3-b] indole (A-α-C), 1-Methyl-9H-pyrido [3, 4-b] indole (Harman), 9H-Pyrido [3, 4-b] indole (Norharman), and 2-Amino-1-methyl-6-phenylimidazo [4, 5-b] pyridine (PhIP), in the urine from adult participants were extracted from the 2013–2014 NHANES database. Propensity score matching (PSM) was used to balance confounding variables between the stone former and non-stone former groups, and logistic regression analysis was performed to analyze the relationship between HAAs and the occurrence of kidney stones.ResultsOf the 1,558 eligible participants, a history of kidney stones was self-reported by 140 (9.0%). Compared to non-stone formers, stone formers had higher concentrations of A-α-C, Harman, and Norharman and lower concentrations of PhlP in urine. After adjusting for all other confounding variables, multivariate logistic regression analysis showed that the high-Harman group had a higher risk of kidney stones than the low-Harman group [adjusted odds ratios (aOR) = 1.618, 95% CI: 1.076–2.433, p = 0.021]. After PSM analysis, Harman concentration remained a risk factor for kidney stones (high-Harman group vs. low-Harman group: aOR = 1.951, 95% CI: 1.059–3.596, p = 0.032).ConclusionIncreased urinary Harman concentrations are associated with an increased risk of kidney stones in the general US population.

Background: Genomic technologies can be subject to significant batch-effects which are known to reduce experimental power and to potentially create false positive results. The Illumina Infinium Methylation BeadChip is a popular technology choice for epigenome-wide association studies (EWAS), but presently, little is known about the nature of batch-effects on these designs. Given the subtlety of biological phenotypes in many EWAS, control for batch-effects should be a consideration. Results: Using the batch-effect removal approaches in the ComBat and Harman software, we examined two in-house datasets and compared results with three large publicly available datasets, (1214 HumanMethylation450 and 1094 MethylationEPIC BeadChips in total), and find that despite various forms of preprocessing, some batch-effects persist. This residual batch-effect is associated with the day of processing, the individual glass slide and the position of the array on the slide. Consistently across all datasets, 4649 probes required high amounts of correction. To understand the impact of this set to EWAS studies, we explored the literature and found three instances where persistently batch-effect prone probes have been reported in abstracts as key sites of differential methylation. As well as batch-effect susceptible probes, we also discover a set of probes which are erroneously corrected. We provide batch-effect workflows for Infinium Methylation data and provide reference matrices of batch-effect prone and erroneously corrected features across the five datasets spanning regionally diverse populations and three commonly collected biosamples (blood, buccal and saliva). Conclusions: Batch-effects are ever present, even in high-quality data, and a strategy to deal with them should be part of experimental design, particularly for EWAS. Batch-effect removal tools are useful to reduce technical variance in Infinium Methylation data, but they need to be applied with care and make use of post hoc diagnostic measures. [ABSTRACT FROM AUTHOR]

In this article, we discuss the insights object-oriented ontology (OOO) offers in understanding the photographic process. Following Kant's distinction between noumena and phenomena and Heidegger's Geviert, Harman's OOO focuses on the real versus sensory aspects of all objects of experience. In our analysis, we explore its implications for intentionality, signification and revelation in photography. OOO locates being within all objects and stands in opposition to the post-Cartesian correlationism influential in the continental tradition. In Heidegger's terms, the still camera exhibits both presence and readiness at hand. However, this readiness at hand is so ubiquitous that it is easy to overlook what makes it unique amongst the tools at our disposal. We argue that in the field of visual experience, the camera does what Kant believed impossible. It has the potential to reduce epistemic loss and transform the phenomenology of conscious experience into the noumenology of non-conscious awareness of the real object. [ABSTRACT FROM AUTHOR]

The paper aims to examine, in terms of gender, the needs determining the division of labor and subsequently to discuss common working areas as the result of this division of labor. The research developed based on the working life and gender roles of the villagers living in Sivri Dere, Oltu district. The ethnographic method and observation were used as data collection tools. The research determined places mostly used as common areas and their uses according to gender. Thus, women mostly use dairies, tandoori, ovens, houses, fountains and springs as common working areas. On the other hand, men mostly use flooded places, adjacent areas of the mosque, road joints, pastures, forests, cattle pen, shelters, sheepfolds, crossroads, water channels and mills as commo working areas. Trashing areas, on the other hand, are used by both women and men as a collaborative working area. [ABSTRACT FROM AUTHOR]

The β-carbolines in our diet, mainly including harman and norharman, are a group of biologically active, naturally occurring plant-derived alkaloids. Fragrant sesame seed oil is one of the most popular flavor edible oils in China. Considering that sesame seeds are roasted at 200–240 °C during the processing of flavor sesame seed oils, it is meaningful to investigate the levels of β-carboline compounds in various sesame seed oils. In this work, the levels of β-carbolines (harman and norharman) in different types of sesame seed oils in China (e.g., pressed fragrant sesame oil, ground fragrant sesame oil) have been determined systematically. The results showed that the levels of total β-carbolines in pressed fragrant sesame oils (700.5~2423.2 μg/kg) were higher than that in ground fragrant sesame oils (660.4~1171.7 μg/kg). Roasting sesame seeds at high temperatures (200–240 °C) led to higher levels of β-carbolines (660~2400 μg/kg) in fragrant sesame seed oils. In addition, the loss of tryptophan might be attributed to the formation of β-carbolines in sesame seeds during the roasting process. In general, fragrant sesame seed oils (pressed fragrant sesame oils, ground fragrant sesame oils) contain higher levels of β-carbolines due to the formation of harman and norharman during the roasting sesame seed process.

Heterocyclic aromatic amines (HAAs) are a class of hazardous compounds produced in food thermal processing. These compounds raise concerns because they have mutagenic and carcinogenic properties. However, the neurotoxicity of these compounds has received limited attention. Here, the toxic effects of three HAAs, i.e. 9H-pyrido[3,4- b ]indole (Norharman), 1-methyl-9H-pyrido[3,4- b ]indole (Harman), and 2-amino-3-methylimidazole[4,5- f ]quinoline (IQ) were investigated in Neuro-2a cells model. The results showed that the survival rate of cells decreased in a dose-dependent manner and apoptosis occurred after exposure to the three HAAs for 24 h and 48 h. Their neurotoxicity was ranked as Harman > Norharman > IQ. Further, treatment of Harman, Norharman, or IQ at 50 and 100 μM for 48 h led to intracellular REDOX imbalance, which was manifested as increased ROS and malondialdehyde (MDA) levels, decreased GSH/GSSG ratio, and reduced SOD and CAT activities. Moreover, Norharman and Harman up-regulated the expression level of nuclear factor erythroid 2-related factor 2 (Nrf2), as well as the mRNA levels of Heme oxygenase-1 (HO-1) and NAD(P)H quinone oxidoredutase1 (NQO1), while IQ had no significant effect on the levels of Nrf2, HO-1, and NQO1. Additionally, Harman, Norharman, or IQ exposure significantly reduced mitochondrial membrane potential and intracellular ATP levels and up-regulated the levels of apoptosis-related genes and proteins. Collectively, our finding suggested that HAAs were neurotoxic, with mechanisms related to induction of oxidative stress and mitochondrial dysfunction. [ABSTRACT FROM AUTHOR]

Monoamine oxidase (MAO) enzymes (MAO A and B) catalyze the oxidative deamination of biogenic amines, neurotransmitters, and xenobiotic amines and contribute to the regulation of the content of these active substances in mammalian organisms. The oxidation of biogenic amines by MAO produces hydrogen peroxide (H 2 O 2 ) and aldehydes that represent risk factors for oxidative injury. The inhibitors of MAO are useful as antidepressants and neuroprotective agents. Usually, the assays of MAO determine amine deamination products or measure the H 2 O 2 released by using direct spectrophotometric or fluorimetric methods. Direct methods are more prone to interferences and can afford inaccurate results. Those limitations can be avoided by using chromatographic techniques. This work describes a chromatographic method to assay MAO A and MAO B activity by using kynuramine as a nonselective substrate and the subsequent analysis of 4-hydroxyquinoline by RP-HPLC-DAD-fluorescence and mass spectrometry (MS). Alternatively, the assay uses the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) neurotoxin as a substrate of MAO that is oxidized (bioactivated) to neurotoxic pyridinium cations which are analyzed by HPLC. These methods are applied to assess the inhibition of MAO by bioactive β-carboline alkaloids occurring in foods, plants, and biological systems., (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)

The β-carbolines in our diet, mainly including harman and norharman, are a group of biologically active, naturally occurring plant-derived alkaloids. Fragrant sesame seed oil is one of the most popular flavor edible oils in China. Considering that sesame seeds are roasted at 200–240 °C during the processing of flavor sesame seed oils, it is meaningful to investigate the levels of β-carboline compounds in various sesame seed oils. In this work, the levels of β-carbolines (harman and norharman) in different types of sesame seed oils in China (e.g., pressed fragrant sesame oil, ground fragrant sesame oil) have been determined systematically. The results showed that the levels of total β-carbolines in pressed fragrant sesame oils (700.5~2423.2 μg/kg) were higher than that in ground fragrant sesame oils (660.4~1171.7 μg/kg). Roasting sesame seeds at high temperatures (200–240 °C) led to higher levels of β-carbolines (660~2400 μg/kg) in fragrant sesame seed oils. In addition, the loss of tryptophan might be attributed to the formation of β-carbolines in sesame seeds during the roasting process. In general, fragrant sesame seed oils (pressed fragrant sesame oils, ground fragrant sesame oils) contain higher levels of β-carbolines due to the formation of harman and norharman during the roasting sesame seed process. [ABSTRACT FROM AUTHOR]