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The world's one of the major causes of death are cancer. Cancer is still a complex disease over the years that needs to be cured. Traditional cytotoxic approaches, although they have been implemented for years for treating neoplastic diseases, yet are limited due to the intricacy and low efficiency of cancer cells. Researchers are thus compelled to seek more potent therapeutic strategies. Chimeric antigen receptor (CAR-T) cell therapy is one such innovative insight where T lymphocytes are altered genetically to target cancer cells. Despite the outstanding accomplishment in patients with haematological malignancies, CAR-T cell treatment has demonstrated minimal impact on solid tumours due to a number of obstacles, including proliferation, stability, trafficking, and fate within tumors. Furthermore, interactions between the host and tumour microenvironment with CAR-T cells significantly alter CAR-T cell activities. Designing and implementing these treatments additionally also requires a complex workforce. Overcoming these significant challenges, there is a requirement for innovative strategies for developing CAR-T cells with greater anti-tumour efficacy and reduced toxicity. In this chapter, the current advancement in CAR-T cell technology in order to increase clinical efficacy in both solid tumors and haematological, as well as possibilities to conquer the limits of CAR-T cell therapy in both solid and haematological tumours has been discussed., (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)

Sunshinamide, a cyclodepsipeptide, has demonstrated significant potential in inhibiting cancer cell proliferation. Our prior research established the total synthesis and anticancer properties of sunshinamide. However, a deeper understanding of the structure-activity relationship (SAR) of sunshinamide remained imperative. In this study, we aimed to elucidate the SAR and mechanistic insights underlying sunshinamide action, both in vitro and in vivo. SAR studies confirm the crucial roles of both the bicyclic-ring and disulfide moiety in the anticancer activity of sunshinamide. Our recent findings unveil that sunshinamide targets TrxR1, leading to ROS generation and ER-stress-mediated apoptosis, while also promoting lipid peroxidation by targeting Gpx4, rendering cancer cells vulnerable to ferroptosis. In vivo, experiments demonstrated the effectiveness of sunshinamide in reducing tumor growth by inducing both apoptosis and ferroptosis. The dual efficacy of sunshinamide in eliciting apoptosis and ferroptosis positions it as a promising candidate for breast cancer therapy, addressing the challenge of chemoresistance.

Triple-negative breast cancer (TNBC) is highly aggressive and metastatic in nature. Existing treatment modalities for TNBC are associated with severe side effects. Thioredoxin reductase (TRXR), the pivotal component of the thioredoxin system, remains overexpressed in various cancer cells including TNBC; promotes cell growth, proliferation, and metastasis, and inhibits apoptosis. Pestalotioprolide E is one of the potent macrolides, a class of secondary metabolites derived from an endophytic fungus Pestalotiopsis microspora with relatively unexplored biological activities. Our study revealed increased expression and activity of TRXR1 in MDA-MB-231 cells compared to the non-cancerous cells. In silico docking analysis and in vitro activity assay demonstrated that Pestalotioprolide E directly interacts with TRXR1 and inhibits its enzymatic activity. This inhibition induces apoptosis via TRX1/ASK1/P38MAPK death signaling cascade and retards metastasis through modulating VEGF, MMP-2, MMP-9, E-cadherin, N-cadherin in MDA-MB-231 cells. Taken together present study establishes TRXR1 as a molecular target for Pestalotioprolide E and its anticancer effect can be attributed to the inhibition of TRXR1 activity in MDA-MB-231., Competing Interests: Declaration of competing interest The author declares no competing financial interest., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Monoculture-based microalgae cultivation systems to treat wastewater are well-reported. Despite that, this method has some limitations in terms of nutrient removal potential, environment adaptation, and low biomass productivity. Conversely, microalgae co-cultivation and a two-stage sequential cultivation system (TSSCS) recently emerged as a promising approach to improve the treatment process and biomass productivity through better adaptation to the environment. However, no outdoor large-scale experiments were reported using this approach which hinders the viability of the process. Thus, in the present study, a sequential two-stage large-scale outdoor novel microalgae consortia experiment was developed. In first stage consortia-assisted sequential cultivation, two ratios of Tetraselmis indica (TS) and one ratio of Picochlorum sp. (PC) (2 TS:1 PC) were cultivated in a 1000-L pond containing 75%-municipal wastewater (MWW) + 25%-ASN-III, while in the second stage, 2 PC:1 TS was cultivated in two different ponds, and each containing 375-L 2 TS:1 PC-treated water + 375-L ASN-III. Outdoor parameters and nutrient removal efficiency (NRE), biomass, and biomolecule productivity such as lipid, photosynthetic pigments, astaxanthin, and β-carotene were quantified, and cost analysis was performed. At the end of the first and second stages, 2 TS:1 PC and 2 PC:1 TS showed maximum NRE of COD (68.71 and 86.40%), TN (66.98 and 94.73%), and TP (82.70 and 94.36%), respectively. Moreover, 2 TS:1 PC and 2 PC:1 TS Pond 1 and 2 produced maximum dry biomass production; 2.41 and ∼2.54 g/L contained lipid content; 36.89 and 34.90% that have 86.50 and 55.79% FAME content respectively. Similarly, 2 TS:1 PC and 2 PC:1 TS biomass exhibited valuable pigments production of astaxanthin i.e., 0.56 and 0.35 mg/g, and β-carotene; 4.65 and 2.82 mg/g, respectively. The cost analysis suggested that only microalgal-based MWW treatment was unfeasible, while valorization of produced biomass into co-products could offset the operation costs and could allow the option for the microalgal-based sustainable approach for the treatment of MWW and recovery of valuable resources., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

A convergent strategy for the first total synthesis of the lipopeptide bacilotetrin C has been developed. The key features of this synthesis include Crimmins acetate aldol, Steglich esterification, and macrolactamization. Twenty-nine variants of the natural product were prepared following a systematic structure-activity relationship study, where some of the designed analogues showed promising cytotoxic effects against multiple human carcinoma cell lines. The most potent analogue exhibited a ∼37-fold enhancement in cytotoxicity compared to bacilotetrin C in a triple-negative breast cancer (MDA-MB-231) cell line at submicromolar doses. The study further revealed that some of the analogues induced autophagy in cancer cells to the point of their demise at doses much lower than those of known autophagy-inducing peptides. The results demonstrated that the chemical synthesis of bacilotetrin C with suitable improvisation plays an important role in the development of novel anticancer chemotherapeutics, which would allow future rational design of novel autophagy inducers on this template., Competing Interests: The authors declare no competing financial interest., (© 2024 American Chemical Society.)

The advancement in carbon dioxide (CO 2 ) sequestration technology has received significant attention due to the adverse effects of CO 2 on climate. The mitigation of the adverse effects of CO 2 can be accomplished through its conversion into useful products or renewable fuels. In this regard, microalgae is a promising candidate due to its high photosynthesis efficiency, sustainability, and eco-friendly nature. Microalgae utilizes CO 2 in the process of photosynthesis and generates biomass that can be utilized to produce various valuable products such as supplements, chemicals, cosmetics, biofuels, and other value-added products. However, at present microalgae cultivation is still restricted to producing value-added products due to high cultivation costs and lower CO 2 sequestration efficiency of algal strains. Therefore, it is very crucial to develop novel techniques that can be cost-effective and enhance microalgal carbon sequestration efficiency. The main aim of the present manuscript is to explain how to optimize microalgal CO 2 sequestration, integrate valuable product generation, and explore novel techniques like genetic manipulations, phytohormones, quantum dots, and AI tools to enhance the efficiency of CO 2 sequestration. Additionally, this review provides an overview of the mass flow of different microalgae and their biorefinery, life cycle assessment (LCA) for achieving net-zero CO 2 emissions, and the advantages, challenges, and future perspectives of current technologies. All of the reviewed approaches efficiently enhance microalgal CO 2 sequestration and integrate value-added compound production, creating a green and economically profitable process., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)

Municipal wastewater (MWW) was treated by a sequential pilot microalgal cultivation process. The cultivation was performed inside a specifically designed low-cost photobioreactor (PBR) system. A microalgal consortium 2:1 was developed using Tetraselmis indica (TS) and Picochlorum sp. (PC) in the first stage and PC:TS (2:1) in the second stage and the nutrient removal efficiency and biomass production and biomolecules production was evaluated and also compared with monoculture in a two-stage sequential cultivation system. This study also investigated the effect of seasonal variations on microalgae growth and MWW treatment. The results showed that mixed microalgal consortium (TS:PC) had higher nutrient removal efficiency, with chemical oxygen demand (COD), total phosphate (TP), and total nitrate (TN) removal efficiencies of 78.50, 84.49, and 84.20%, respectively, and produced a biomass of 2.50 g/L with lipid content of 37.36% in the first stage of cultivation under indoor conditions. In the second stage of indoor cultivation, the PC:TS consortium demonstrated maximum COD, TP, and TN removal efficiencies of 92.49, 94.24, and 94.16%, respectively. It also produced a biomass of 2.65 g/L with a lipid content of 40.67%. Among all the seasonal variations, mass flow analysis indicated that the combination of mixed consortium-based two-stage sequential process during the winter season favored maximum nutrient removal efficiency of TN i.e. 88.54% (84.12 mg/L) and TP i.e., 90.18% (43.29 mg/L), respectively. It also enhanced total biomass production of 49.10 g in 20-L medium, which includes lipid yield ∼15.68 g compared to monoculture i.e., 82.06% (78.70 mg/L) and 82.87% (40.26 mg/L) removal of TN and TP, respectively, and produced biomass 43.60 g with 11.90 g of lipids., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

A systematic structure-activity relationship study of the potent anticancer marine macrolide biselyngbyolide B has been accomplished. A total of 11 structural variants of the parent natural product, of which 2 are natural analogues, have been studied against a human colorectal carcinoma cell line. The requisite functional units of the parent molecule responsible for the cytotoxic activities have been disclosed. Biselyngbyolide C, one of the natural analogues of biselyngbyolide B, has been studied in depth to explore its molecular mechanism. Interestingly, the in vitro data demonstrated an induction of dynamin-related protein 1-mediated mitochondrial fission and reactive oxygen species production which led to activation of ASK1/P38/JNK-mediated apoptosis in colon cancer cells as an important pathway for biselyngbyolide B-mediated cytotoxicity. Notably, this study revealed that a macrolide participated in mitochondrial fission to promote apoptosis of cancer cells, providing new insight., Competing Interests: The authors declare no competing financial interest., (© 2024 American Chemical Society.)

A convergent strategy for the stereoselective synthesis of the methyl ester of the structurally challenging and highly labile antibacterial polyene polyketide natural product thailandamide A has been developed. The key steps include the Zincke aldehyde reaction, Stille cross coupling, Negishi reaction, Julia-Kocienski olefination, cross metathesis, and the less explored Pd(I)-based Heck coupling to access different unsaturation bonds. Additionally, Urpi acetal aldol, Evans methylation, and Crimmins acetate aldol reactions were employed to construct four out of six asymmetric centers of the molecule.

A convergent route for the asymmetric total synthesis of antibacterial macrolide sorangiolide A has been developed for the first time. The key feature of this synthesis includes Krische iridium-catalyzed anti -diastereoselective carbonyl crotylation, Crimmins acetate aldol, Yamaguchi esterification, Julia-Kocienski olefination, Horner-Wadsworth-Emmons olefination, and ring-closing metathesis. The origin of the low intensity of the 13 C{ 1 H} NMR signals of the C1 and C2 centers of the natural product has been investigated, disclosing possible forms of existence for the natural product in the solution phase.

Microalgal-based bioprocess offers several advantages including wastewater reclamations, therefore present study assessed the usability of the combination of untreated municipal sewage wastewater (UTMSWW) and secondary treated municipal sewage wastewater (STSWW) for nutrient removal and recovery by Tetraselmis indica ( T . indica ) BDUG001. The present study optimized the additional nutrient supplementations (e.g. ASN-III) percentage and day-night cycle, pH and pH with aeration for monitoring high-rate biomass production and nutrient recovery. The study results showed that the combination of 75% UTMSWW + 25% ASN-III supported maximum biomass production (2.65 ± 0.07 g/L). In the optimized day-night cycle (12:12 h), T. indica BDUG001 showed improved biomass production (2.75 ± 0.07 g/L), biomass productivity (165.63 ± 4.42 mg/L/d), and photosynthetic pigments production. Under optimized pH∼ 7.0 with aeration, maximum total nitrate (TN) removal efficiency (87.67 ± 3.08-91.55 ± 1.92%) was observed, while COD and TP removal was maximum at pH ∼ 9.0. The maximum biomass production (2.35 ± 0.07-2.77 ± 0.04 g/L) with biomass productivity (93.75 ± 167.19 ± 2.21 mg/L/d) and lipid content (42.98 ± 1.86-47.85 ± 0.21% DCW) were also at pH 7.0. with aeration. The present study verified the utilization of UTMSWW with the combination of conventional medium, optimized day-night cycle, pH with aeration along with designing low-cost PBR. It was the ideal system for the cultivation of T. indica BDUG001 for the recovery of nutrients from wastewater, production of biofuels and value-added feedstock.

Stereoselective total synthesis of the structurally intriguing polyketide natural product thailandamide lactone was accomplished, and done so using a convergent approach for the first time to the best of our knowledge. The key features of this synthesis included use of a Crimmins acetate aldol reaction, Evans methylation, Urpi acetal aldol reaction, Sharpless asymmetric epoxidation and subsequent γ-lactonization for the installation of six asymmetric centers and the use of the Negishi reaction, Julia-Kocienski olefination, cross metathesis, HWE olefination and intermolecular Heck coupling for construction of a variety of unsaturated linkages. Pd(i)-based Heck coupling was introduced, for the first time to the best of our knowledge, quite efficiently to couple the major eastern and sensitive western segments of the molecule. The antibacterial activity of thailandamide lactone was also evaluated., Competing Interests: There are no conflicts to declare., (This journal is © The Royal Society of Chemistry.)

Asymmetric total synthesis of cyclotetradepsipeptide beauveamide A has been achieved for the first time. A macrolactamization strategy involving two possible sites has been explored to find the most effective route for cyclization. A late-stage functionalization approach has been adopted for easy access of non-natural analogues of beauveamide A for further biological evaluation. Interestingly, the anticancer activity of one of the synthesized analogues was better than that of the parent natural product.

Heavy metals-containing drinking water and wastewater are posing a severe threat to the environment, and living beings on land, air and water. Different conventional, advanced nanomaterials-based and biological method has been employed for the treatment of heavy metals. Among the biological methods, microalgae are an important group of micro-organisms that have numerous environmental applications and can remediate heavy metals from wastewater. Also, it has numerous advantages over conventional remediation processes. Microalgae cells can uptake the heavy metal via different physiological and biological methods and are utilized as a nutrient source to regulate its metabolic process for the production of biomass. Furthermore, the enhancement in heavy metal removal efficiency can be improved using different strategies such as immobilization of algal cells, development of algal consortia and designing of microalgae-based nanocomposite materials. Also, it can significantly contribute towards environmental sustainability and future. Thus, the review provides a critical overview of heavy metals and their existence along with their negative effects on humans. This review provides insight on recent advanced nanomaterial approaches for the removal of heavy metals, overviews of microalgae-based heavy metal uptake mechanisms and their potential for the amputation of different heavy metals. Furthermore, the special focus is on recent strategies that enhance heavy metal removal efficiency and contribute towards sustainability for the development of a microalgae-based future., (© 2021 The Society for Applied Microbiology.)

Stereoselective total synthesis of structurally intriguing antimalarial macrolide strasseriolide A has been accomplished by adopting a convergent approach. The salient features of this synthesis include Co(BH 4 ) 2 -mediated selective reduction of conjugated olefin, Crimmins propionate aldol, Evans alkylation, intermolecular Horner-Wadsworth-Emmons olefination, Yamaguchi macrolactonization, and selective saponification of ester moiety in the presence of a lactone functionality. The 13 C{ 1 H} NMR data of strasseriolide A were found to be very sensitive to its solution concentration.

Urbanization is a revolutionary and necessary step for the development of nations. However, with development emanates its drawback i.e., generation of a huge amount of wastewater. The existence of diverse types of nutrient loads and toxic compounds in wastewater can reduce the pristine nature of the ecosystem and adversely affects human and animal health. The conventional treatment system reduces most of the chemical contaminants but their removal efficiency is low. Thus, microalgae-based biological wastewater treatment is a sustainable approach for the removal of nutrient loads from wastewater. Among various microalgae, Tetraselmis sp. is a robust strain that can remediate industrial, municipal, and animal-based wastewater and reduce significant amounts of nutrient loads and heavy metals. The produced biomass contains lipids, carbohydrates, and pigments. Among them, carbohydrates and lipids can be used as feedstock for the production of bioenergy products. Moreover, the usage of a photobioreactor (PBR) system improves biomass production and nutrient removal efficiency. Thus, the present review comprehensively discusses the latest studies on Tetraselmis sp. based wastewater treatment processes, focusing on the use of different bioreactor systems to improve pollutant removal efficiency. Moreover, the applications of Tetraselmis sp. biomass, advancement and research gap such as immobilized and co-cultivation have also been discussed. Furthermore, an insight into the harvesting of Tetraselmis biomass, effects of physiological, and nutritional parameters for their growth has also been provided. Thus, the present review will broaden the outlook and help to develop a sustainable and feasible approach for the restoration of the environment., (© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Cananginones, a family of linear acetogenins found as secondary metabolites in the plant kingdom, show cytotoxicity against several types of cancer cells. We aimed to investigate the efficacy of cananginone and its mechanism as an anti-cancer agent. Our initial screening of Cananginone against HepG2, PC3, A549, and MCF7 cells showed anti-cancer activities and is more potent against MCF7 cells, consistent with the previous report. Next, cell-based assays have revealed that cananginone abrogates cancer stem cell renewal as well as Epithelial-Mesenchymal Transition (EMT) and increased the ROS level beyond the threshold level thus reducing the viability of cancer cells. In the connection of Hh-Gli to EMT, our study indicated that cananginone inhibits Gli1 in a non-canonical pathway. Presumably, this is the first report on the inhibitory activity of cananginone in the Hh pathway and is different from Hh-antagonists cyclopamine and GANT 61 considering the mechanism., (© 2022 Wiley-VHCA AG, Zurich, Switzerland.)

Asymmetric total synthesis of an acetate analogue of the endophytic unstable secondary metabolite bipolamide A has been achieved for the first time adopting a convergent approach. The key feature of this synthesis includes Evans's asymmetric ethylation, Wittig olefination, Takai olefination, stereoselective Grignard addition and intermolecular Heck coupling. This eventually developed a synthetic route of the rarely found branched amine bearing an acyloin moiety. Our synthesis finally established unambiguously the stereochemistry of the unassigned C-8 center of the naturally occurring unstable bipolamide A.

The freshwater macrophytes are abundant in tropical and subtropical climates. These macrophytes may be used as feed ingredients for fish and other animals. The nutritional value of twelve freshwater-cultured macrophytes was evaluated in the present study. Significantly higher crude protein (36.94-36.65%) and lipid (8.13-7.62%) were found in Lemna minor and Spirodela polyrhiza ; ash content was significantly higher in Hydrilla verticillata, Wolffia globosa , and Pistia stratiotes (20.69-21.00%) compared with others. The highest levels of sodium, magnesium, chromium, and iron levels were recorded in P. stratiotes . H. verticillata was a rich source of copper, manganese, cobalt, and zinc; the contents of calcium, magnesium, strontium, and nickel were highest in S. polyrhiza . Selenium and potassium contents were higher in Salvinia natans and W. globosa , respectively. The n-6 and n-3 polyunsaturated fatty acids (PUFAs) contents were significantly higher in W. globosa and Ipomoea aquatica , respectively compared with others. Linoleic and α-linolenic acids were dominant n-6 and n-3 PUFAs. The highest value (4.04) of n-3/n-6 was found in I. aquatica . The ratio ranged from 0.61 to 2.46 in other macrophytes. This study reveals that macrophytes are rich sources of minerals, n-6 and n-3 PUFAs., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Kumar, Sharma, Goswami, Shrivastav, Tocher, Kumar and Chakrabarti.)

Field experiments were conducted at Biswanath, Assam, India (26° 42' N and 93° 15' E), during 2016, 2017, and 2018, to evaluate the effect of microclimates on growth, yield, and disease incidence in the ginger crop. The ginger variety Nadia was grown under six microclimates, viz., under shade net for the entire crop season (T 1 ), under shade net from planting to mid-October (T 2 ), with pigeon pea (T 3 ), with maize (T 4 ), with okra (T 5 ), and as a sole crop (T 6 ) in three replicated RBD. Photosynthetically active radiation (PAR), net radiation (R n ), temperature above the ginger canopy, soil temperature, and soil moisture were measured during the critical crop growth period under different microclimates. Recording of rhizome rot disease incidence was done periodically and genomic analysis of pathogen was carried out. PAR recorded above the ginger canopy under T 6 was 1688.1 μ mol s -1  m -2 , which was attenuated up to 80.1% in other microclimates. The R n load of the ginger canopy was maximum (446.4 W m -2 ) under T 6 , which reduced to below 50 W m -2 under both T 3 and T 4 . Both air temperatures above the ginger canopy and soil temperatures under T 3 and T 4 were reduced by 3.3 °C and 4.6 °C, respectively, as compared to T 6 . The pathogen causing the disease in the experimental site was identified as Fusarium oxysporum. Considerable increase in soil and air temperature and soil moisture favored disease incidence (90.3%) under shade net (T 1 and T 2 ) treatments, while opposite reason causing significant reduction in disease incidence (16.1%) was observed under T 3 and T 4 . More yield of ginger recorded in treatments T 3 (6.21 t ha -1 ) or T 4 (6.48 t ha -1 ) was attributed to better crop growth and diminutive disease incidence, while the crop was almost damaged due to severe disease incidence under shade net (T 1 and T 2 ) treatments., (© 2021. The Author(s) under exclusive licence to International Society of Biometeorology.)

The major downfalls of the microalgal biorefinery are low volume of high value product accumulation, low biomass productivity and high cultivation costs. Here, we aimed to improve the biomass productivity of the industrially relevant Picochlorum sp. BDUG 100241 strain. The growth of Picochlorum sp. BDUG 100241 was investigated under different cultivations conditions, including photoautotrophic (with light), mixotrophic (1% glucose, with light) and heterotrophic (1% glucose, without light). Among them, Picochlorum sp. BDUG100241 showed the highest growth in the mixotrophic condition. Under different (1%) carbon sources' supplementation, including glucose, sodium acetate, glycerol, citric acid and methanol, Picochlorum sp. BDUG100241 growth was tested. Among them, sodium acetate was found to be most suitable carbon source for Picochlorum sp. BDUG 100241 growth, biomass (1.67 ± 0.18 g/L) and biomolecule productivity. From the different concentrations of sodium acetate (0, 2.5, 5.0, 7.5 and 10 g/L) tested, the maximum biomass production of 2.40 ± 0.20 g/L with the biomass productivity of 95 ± 5.00 mg/L/d was measured from 7.5 g/L in sodium acetate. The highest total lipid (53.50 ± 1.70%) and total carotenoids (0.75 ± 0.01 µg/mL) contents were observed at the concentration of 7.5 g/L and 5.0 g/L of sodium acetate as a carbon source, respectively. In conclusion, the mixotrophic growth condition containing 7.5 g/L of sodium acetate showed the maximum biomass yield and biomolecule accumulation compared to other organic carbon sources.

The potential nutritional value of duckweed Lemna minor (Lemnaceae) was evaluated for common carp Cyprinus carpio fry. Fish were fed diets containing five graded levels of duckweed: 0% (LM0, control), 5% (LM5), 10% (LM10), 15% (LM15) and 20% (LM20). The final weight and specific growth rate were significantly higher in LM15 and LM20 diets fed fish compared to others. Feed conversion ratio was minimum in fish fed diet LM20. Amylase activity was significantly higher in LM0 treatment. Total protease, trypsin and chymotrypsin activities showed linear relationships with the increased level of duckweed in the diet. Protein and essential amino acids contents were significantly higher in carp fed diets LM15 and LM20 compared to others. Lipid content was significantly higher in fish fed duckweed-based diets compared to control. A direct relationship was found between the inclusion level of duckweed in the diet and n-3 long-chain polyunsaturated fatty acid (LC-PUFA) content of carp. Contents of desaturated and elongated products of dietary linolenic acid (18:3n-3) including 20:4n-3, 20:5n-3, 22:5n-3 and 22:6n-3 increased in a graded manner with increasing dietary duckweed. The monounsaturated fatty acids and n-6 PUFA contents reduced significantly in fish fed duckweed. Expression of fads2d6, elovl2, elovl5 and fas were higher in carp fed diets LM10, LM15 and LM20 compared to control fish. The inclusion of L. minor in diet enhanced the nutritional value of carp by increasing protein, lipid, amino acids and n-3 PUFA contents., (© 2022. The Author(s).)

The wastewater originates from different industrial, municipal, and agriculture processes and contains different nitrogen sources, for example, nitrate, ammonium, nitrite, and phosphate such as inorganic and organic sources. The discharge of high nitrate and phosphate to the ecosystem or nearby water bodies can cause eutrophication which disbalances the aquatic ecosystem. Furthermore, ingestion of these pollutants can cause severe toxicity and disease to humans and animals. Thus, from an environmental and social perspective, its treatment is essential with no negative impact on the ecosystem. Microalgae are fundamental, mixotrophic microorganisms that treat different wastewater and utilize nitrate and phosphate in the medium as a source of nutrients. Among them, Picochlorum sp., have the potential to remove nitrogen and phosphate from wastewater. The biomass produced by Picochlorum sp. can be a promising candidate as a sustainable feedstock for biofuel and bioproducts formation. Thus, the present review provides a brief knowledge and understanding about the concentration of nitrogen and phosphate in different wastewater, their negative impacts, and the uptake mechanism of microalgae. Furthermore, the review also provides an insight into Picochlorum sp., and the effects of different physiological and nutritional factors on their growth, wastewater treatment efficacy, and biomass for value-added products and biorefinery applications. In addition, the review is useful to understand the potential of Picochlorum sp. for a tenable wastewater treatment process., (© 2021 Wiley-VCH GmbH.)