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Oxygen isotopes in biogenic silica (δ18OBSi) from lake sediments allow for quantitative reconstruction of past hydroclimate and proxy–model comparison in terrestrial environments. The signals of individual records have been attributed to different factors, such as air temperature (Tair), atmospheric circulation patterns, hydrological changes and lake evaporation. While every lake will have its own set of drivers of d18O, here we explore the extent to which regional or even global signals emerge from a series of palaeoenvironmental records. For this purpose, we have identified and compiled 71 down–core records published to date and complemented these datasets with additional lake basin parameters (e.g. lake water residence time and catchment size) to best characterize the signal properties. Records feature widely different temporal coverage and resolution ranging from decadal–scale records covering the last 150 years to records with multi–millennial scale resolution spanning glacial–interglacial cycles. Best coverage in number of records (N = 37) and datapoints (N = 2112) is available for northern hemispheric (NH) extra–tropic regions throughout the Holocene (corresponding to Marine Isotope Stage 1; MIS 1). To address the different variabilities and temporal offsets, records were brought to a common temporal resolution by binning and subsequently filtered for hydrologically open lakes with lake water residence times < 100 yrs. For mid– to high–latitude (> 45° N) lakes, we find common δ18OBSi patterns during both the Holocene and the Common Era and maxima and minima corresponding to known climate episodes such as the Holocene Thermal Maximum (HTM), Neoglacial Cooling, Medieval Climate Anomaly (MCA) and the Little Ice Age (LIA). These patterns are in line with long–term Tair changes supported by previously published climate reconstructions from other archives as well as Holocene summer insolation changes. In conclusion, oxygen isotope records from NH extratopic lake sediments feature a common climate signal at centennial (for CE) and millennial (for Holocene) time scales despite stemming from different lakes in different geographic locations and constitute a valuable proxy for past climate reconstructions.

Isotopes in diatoms are increasingly used in palaeoenvironmental studies in both lacustrine and marine settings, enabling the reconstruction of a range of variables including temperature, precipitation, salinity, glacial discharge, carbon dynamics and biogeochemical cycling. This protocol details an optimised methodology for extracting diatoms for isotope analysis from sediment samples, using a range of chemical and density separation techniques that minimise sample loss and avoids the need for expensive equipment. Whilst designed for the extraction of diatoms for oxygen, silicon and carbon isotope analysis, additional stages are outlined for the analysis of other isotopes that are of increasing interest to the palaeo community (e.g., boron and zinc). The protocol also includes procedures for assessing sample purity, to ensure that analysed samples produce robust palaeoenvironmental reconstruction. Overall, the method aims to improve the quality of palaeoenvironmental research derived from isotopes in diatoms by maximising sample purity and the efficiency of the extraction process.