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Pneumonic pasteurellosis is the leading cause of recurrent morbidity and mortality in ruminant livestock in Ethiopia. Its control is mainly done using an annual vaccination with a monovalent whole broth culture of Pasteurella (P.) multocida. However, the multiplicity of the serotypes circulating in the field and the lack of cross-protective immunity hinder the effectiveness of the vaccination program warranting the development of a vaccine with better efficacy. To this effect, the identification, and characterization of the strains from different regions of the country is necessary. In this paper, Pasteurella organisms collected from camels, cattle, goats, and sheep with respiratory signs suggestive of pasteurellosis in the Afar, Tigray, and Benishangul Gumuz regional states were isolated, and characterized. From clinically pneumonic cases, 793 nasal swabs (286 goats, 276 sheep, 168 camels, and 63 cattle) were collected aseptically and cultured on Blood Agar. Bacteria pathogens were identified at the species level by biochemical tests. Culture positivity was 29.3% (243/793). The isolation frequencies of B. trehalosi, M. haemolytica, and P.multocida were 47.7% (116/243), 43.2% (105/243), and 9.1% (22/243), respectively. A higher isolation rate was observed in sheep (37.4%), and the lowest in cattle (6.2%). Mixed infection with B. trehalosi and M. haemolytica was observed in sheep, goats, and camels. Despite the higher frequencies of isolation of B. trehalosi and M. haemolytica from all host species, the vaccine currently being used in Ethiopia consists of only P. multocida biotypes A for sheep and goats and biotype B for cattle. Moreover, camels are not considered in the pasteurellosis vaccination program in the country. Therefore, the result of the study suggests the need to include B. trehalosi and M. haemolytica in the vaccine preparation as well as underlines the relevance of considering camels in the vaccination program. [ABSTRACT FROM AUTHOR]

Introduction: Lumpy skin disease (LSD) is viral disease affecting cattle production and productivity in Ethiopia. As a prevention method, vaccinations have been used for a long period with a questionable output due to the existence of LSD outbreaks in vaccinated herds in different parts of Ethiopia., Methods: A longitudinal study was performed from October 2019 to April 2020 with the objective of assessing the humoral immune response of cattle with a serum neutralization test (SNT) from different management systems in central Ethiopia. In this study, theserum was collected from 113 cattle (extensive (60/113) and intensive (53/113) management systems) before and after vaccination., Results and Discussion: From collected sera, a limited number of cattle had seroconversion before vaccination (7.08%). On the other hand, it is obvious the seroconversion rises post vaccination. Accordingly, seroconversion starts to increase after a week (8.85% at 7 dpv) post-vaccination which proceeds to significantly increase at 30 days post vaccination (dpv) (41.65% (25/60)). Furthermore, the risk factor study before and after vaccination showed intensively managed cattle with significantly higher levels of antibody titer at 7 dpv (OR = 1.17; 95% CI = 0.22, 6.2; p = 0.016) and 30 dpv (OR = 3.67; 95% CI = 1.1, 12.29; p = 0.035) compared with that of extensively managed cattle. The other animal-related risk factor that showed a significant difference was breeds and a specific age group ([4½, 7] years) at 15 dpv (OR = 6.69; 95% CI = 2.02, 22.08; p = 0.002) and 30 dpv (OR = 4.24; 95% CI = 1.22, 14.71; p = 0.023); respectively., Conclusion: This study showed an overall lower antibody detection across the study, posing a question on the current LSD-vaccine efficacy. Therefore, a circulating strain of LSDV should be cross-checked with the vaccine strain and adaptations should be made from it., Competing Interests: The authors declare that they have no competing interests., (© 2023 Moje et al.)

Introduction: Foot-and-mouth disease is globally one of the most economically important viral diseases of cloven-hoofed animals that can be controlled by different strategies, where vaccination plays an important role. Selection of adjuvant added to vaccine preparation is crucial in ensuring the protective effect of the vaccine. Aluminum hydroxide gel mixed with saponin (AS) is widely used adjuvant, with its suboptimal immune response in FMD vaccine. The present study was undertaken to evaluate different ingredients of adjuvants for inactivated trivalent (A, O and SAT 2) FMD vaccine and to demonstrate the effect of booster dose in cattle., Methods: Cattle were grouped into five; four experimental and one control, with six animals in each group and immunized with trivalent vaccine with various formulations of adjuvants. Immune response was measured using Solid Phase Competitive Enzyme Linked Immune Sorbent Assay (SPCE)., Results: The antibody level in cattle immunised with a vaccine formulation containing a mixture of aluminum hydroxide gel and saponin (AS) were significantly lower than AS boosted group for the three serotypes (p<0.05, t -test), which directs the need for booster dose. Whereas the antibody response in the AS + oil group was higher followed by oil alone. The AS preparation with a booster dose has shown better immune response compared to the group without., Conclusion: The findings of this study could suggest that oil based and AS with oil could replace the conventional aluminum hydroxide gel and saponin adjuvants in FMD vaccine preparations. Challenge test was not successful indicating the need for further research on the virus infectivity., Competing Interests: The authors declare that there are no conflicts of interest regarding the publication of this paper., (© 2023 Ayele et al.)