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One of the new waters, and environmentally friendly agriculture initiatives in Peru is to encourage the utilization of agricultural waste, because low agricultural output is a threat to food security there. The purpose of this research was to evaluate the effect of harvest residues on the basidiocarp production of the fungus Pleurotus Ostreatus, in Acobamba-Huancavelica. The trial had a completely randomized design, and the treatments included T1, barley stubbles; T2, wheat stubbles; T3, pea stubbles; T4, broad bean stubbles; and T5, quinoa stubbles. The research was quantitative in nature, taking the form of an experiment with an applied, explanatory level of design. The recorded data was tabulated and analyzed with analysis of variance, as well as Tukey's test (α:0.05), for which the statistical software Infostat was used. The results are presented in tables and graphs for a better interpretation. As main results, it was obtained that the time (colonization), diameter (stem, pileus), length (stem) and weight (basidiocarps), present statistical differences between treatments showing significant enhancement in all parameters. Despite a numerical difference, a Tukey average comparison test revealed that there was no statistically significant difference between the averages for the variable time for fungus colonisation, suggesting that the treatment T5 in which quinoa substrate showed the greatest average. Treatment T4 in which broad bean stubbles were used gave the most low-average. In conclusion, increment in all parámeters were noted in all treatment of Pleurotus basidiocarps ostreatus under Acobamba conditions. Resumo Uma das novas iniciativas de águas e agricultura ecológica no Peru é incentivar a utilização de resíduos agrícolas, porque a baixa produção agrícola é uma ameaça à segurança alimentar no país. O objetivo desta pesquisa foi avaliar o efeito dos resíduos da colheita na produção de basidiocarpos do fungo Pleurotus ostreatus, em Acobamba-Huancavelica. O ensaio teve um delineamento inteiramente casualizado, e os tratamentos incluíram T1, restolho de cevada; T2, soqueiras de trigo; T3, restolho de ervilha; T4, restolho de fava; e T5, restolho de quinoa. A pesquisa foi de natureza quantitativa, assumindo a forma de experimento com um nível de design aplicado e explicativo. Os dados registrados foram tabulados e analisados com análise de variância, bem como teste de Tukey (α:0,05), para o qual foi utilizado o software estatístico Infostat. Os resultados são apresentados em tabelas e gráficos para melhor interpretação. Como principais resultados, obteve-se que o tempo (colonização), diâmetro (caule, píleo), comprimento (caule) e peso (basidiocarpos) apresentam diferenças estatísticas entre os tratamentos, mostrando melhora significativa em todos os parâmetros. Apesar da diferença numérica, o teste de comparação de médias de Tukey revelou que não houve diferença estatisticamente significativa entre as médias para a variável tempo de colonização do fungo, sugerindo que o tratamento T5 em que usou o substrato quinoa, apresentou a maior média. O tratamento T4, no qual foram usadas restolhos de fava, apresentou a média mais baixa. Em conclusão, incrementos em todos os parâmetros foram observados em todos os tratamentos de Pleurotus basidiocarps ostreatus nas condições de Acobamba.

Background: Fojo mine (Castelo de Paiva, Douro Carboniferous Basin) was used for anthracite exploitation until 1994, resulting in the production of coal waste piles without any environmental management [1,2]. In October 2017, a wildfire triggered the ignition of some of these waste piles. The extinguishing process of the fire included the remobilization of the coal mining residues using water mixed with a cooling accelerator agent. Objective: To assess the ecotoxicological effects of soil elutriates from the Fojo coal mine waste in seed germination assay with Lactuca sativa. Methods: 25 soil samples were taken: unburned coal waste (ENA); burned coal waste (EA); burned coal waste cover (EAC); mixed material (EE) resulting from the extinguishing process; uphill from the waste pile (B); downhill from the waste pile (SJ). The assay was performed with elutriates from each soil sample and four replicates were prepared per elutriate with 10 seeds. The percentage of germination, fresh biomass, and total size were measured at the end of 14 days. Results: The percentage of seed germination, leaf, and root growth were differently affected revealing high heterogeneity of soil characteristics. The highest seed germination was in EE soil samples (>80%), and the lowest values were in different heterogenic zones (ENA3, ENA5, EAC10, and SJ5). The lowest total size was observed in EE6 (< 4.3 cm) and ENA3 (< 5.3 cm). Leaf fresh biomass and size were higher in soil samples from B zone (1.64 g and 4.64 cm, respectively), and lower values in SJ (0.63 g and 3.34 cm). Conclusions: The results highlight the importance of the phytotoxicity studies with coal mining waste, since the lixiviates may affect the terrestrial ecosystems, reducing plant establishment and growth. Seed germination, and shoot and root elongation proved to be a sensitive endpoint to evaluate the phytotoxicity of coal waste., Scientific Letters, Vol. 1 No. Sup 1 (2023)

The purpose of the present investigation was to report the presence of cervicovaginal microorganisms most frequently found in cervicovaginal exfoliative smears (Papanicolaou) of patients with type 2 diabetes. Cervical-vaginal exfoliative cytologist was performed on an open population to all patients who attended from March 2010 to March 2020 in the within the framework of the early cancer detection program, gynecological interrogations were applied to 1587 patients who, after taking cell samples, were processed using the Papanicolaou staining technique modified by the same for subsequent microscopic diagnosis. Of the results obtained from the 1587 patients studied, 148 (9.32%) suffer from type 2 diabetes mellitus and of these only 130 (87.83%) showed at least one microorganism under microscopic observation, being Candida spp., the most found microorganism in samples from patients with type 2 diabetes. In conclusions, type 2 diabetes is a disease that predisposes to the appearance of microorganisms and cervicovaginal infections, particularly by Candida spp., therefore, there is a need for surveillance guidelines and efforts to reverse this disease and its complications.

Ocular pathologies are, without a doubt, one of the most complicated at the time of regulating medical therapy. Being one of the most relevant sense organs (if not the most important) the use of stem cells in the treatment of metabolic diseases that were previously considered to be slow-progressing, significant improvement can now be achieved.

The gynecological interrogations, clinical impressions and results obtained from 1587 patients who attended the Department of Cellular Biology of the Faculty of Medicine of the Benemérita Universidad Autónoma de Puebla, México, for cervicovaginal exfoliative cytology sampling (Papanicolaou) were analyzed. Within the framework of the early cancer detection program from March 2010 to March 2021. From the 1587 patients studied, 302 reported smoking during the gynecological examination. Of the total number of patients with a smoking habit 178 (58.94%) showed cervicovaginal lesions on microscopic observation and/or physical examination. Smoking is considered a predisposing factor in the appearance of cervicovaginal lesions, and there is a need to carry out more detailed studies on the secondary effects of tobacco in patients with an active sexual life and a smoking habit in Mexico.