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Two field experiments were carried out in 2022 and 2023 within commercial watermelon fields in Stockton and Modesto, CA, USA. Two Trichoderma-containing products were applied to the grafted and nongrafted watermelon seedlings through tray soaking or field chemigation. All seedlings were mechanically transplanted into a split-split plot design with the Trichoderma product as the main factor and application method (tray soaking and chemigation) as the sub-plot. The sub-sub-factor included three interspecific hybrid squash rootstocks (Cucurbita maxima × Cucurbita moschata) 'Cobalt', 'Flexifort', and 'RS841' that were grafted with the commercial seedless watermelon scion 'Summer Breeze'. All treatments were replicated four times. Vine health was visually assessed three times, and canopy coverage was assessed for a total of six measurements for each year. Harvest was conducted three times in 2022 and twice in 2023 to analyze yield and quality differences among treatments. Aboveground and root samples from defective plants were taken amid the harvest and shipped to the University of California, Davis Fungal Pathology Diagnostics Research Laboratory for identification of soilborne fungal pathogens to confirm or rule out their involvement in vine declines initially attributed to nonpathogenic factors. The laboratory diagnosis indicated that Macrophomina was morphologically identified from the foot and root isolations of 67% of the submitted nongrafted, non-Trichoderma-biofungicide-treated plants in 2022. Further sequencing results confirmed the primary pathogen, Macrophomina phaseolina, from 50% of those submitted plant samples. In addition, putative Fusarium root and stem rot (Fusarium oxysporum f. sp. radices-cucurmerinum) and Falciforme crown rot and decline (Fusarium noneumartii) from runners and roots of nongrafted, noninoculated plants in the 2022 experiment were also reported. However, no significant soilborne fungal pathogens were found in the 2023 experiment. In both trials, the effects of Trichoderma-containing biofungicides and their application methods on preventing vine decline, maintaining canopy coverage, and enhancing fruit yield and quality were not as remarkable as grafting. The average grafting effect contributed to 83% and 53% decrease of vine decline compared with the nongrafted plants in 2022 and 2023. Overall, grafted plants yielded 47.6% and 32.4% more than the nongrafted counterpart in 2022 and 2023, respectively. In addition, grafting exerted predominant influence on fruit firmness and rind thickness. The study results indicated that grafting onto multipathogen resistance watermelon rootstocks serves as an effective production tool to maintain fruit yield, quality, and plant health under both pathogenic and disease-free conditions. Further work is still needed to continue evaluating best practical application protocols of Trichoderma-based biofungicides and other biopesticides to enhance product effectiveness and end users' confidence in reducing soilborne diseases and reliance on conventional fumigants. [ABSTRACT FROM AUTHOR]

Summary: The distinct techno‐functional and sensory attributes conferred by milk proteins and fat are fundamental in defining the structure, texture and flavour of dairy products. Thus, reproducing cheese‐like characteristics in plant‐based alternatives while ensuring consumer acceptance is a major challenge. This study aimed to evaluate the sensory profile of commercial cream cheese and plant‐based analogues, quantifying consumer perception and discrimination, and correlating with instrumental texture analysis. For that, two milk‐based (MB1 and MB2) and three plant‐based products (PB1, PB2 and PB3) were evaluated for their proximate composition, texture profile (spreadability and firmness) and sensory properties by combining Flash Profile method (n = 13), Check‐All‐That‐Apply (CATA) test, and Rate‐all‐that‐apply (RATA) test (n = 102) with global acceptance. Milk‐based cream cheeses (MB1 and MB2) did not differ significantly from each other and presented greater spreadability when compared to all plant‐based cream cheeses (PB1, PB2 and PB3). The texture parameters of the samples were inversely related: the greater the firmness, the lower the spreadability. All forty‐two sensory attributes allowed discrimination of the samples into three distinct clusters, with no difference between the milk‐based products. The results of the CATA test showed that the attributes most correlated with the sample MB1 were the most desirable for good acceptance of the product. In turn, PB3 showed lower acceptance scores when compared to the plant‐based samples PB1 and PB2, and the attributes rancid flavour, vegetable oil aroma and nut aroma mostly contributed to the lower acceptance of PB3. Milk‐based products were very close to the ideal product, presenting a creamy and spreadable texture as key attributes for product characterisation and acceptance. The results of RATA test showed a significant difference (P < 0.05) in the intensity of nineteen sensory attributes. Milk‐based cream cheeses were more accepted than their plant‐based counterparts. The instrumental assessments of firmness and spreadability exhibited a negative correlation, once they were inversely proportional and strongly correlated to sensory data of firm and spreadable texture, respectively. The sensory characteristics of cream cheese analogues may present a barrier to their acceptance by consumers. Attributes such as coconut and flour flavour, artificial cheese aroma and flavour, and spreadable texture played a key role in differentiating dairy products from plant‐based ones, contributing to the lower acceptance of the latter. [ABSTRACT FROM AUTHOR]

Strawberries are highly perishable, leading to significant postharvest losses and reduced fruit quality during storage and distribution. Conventional preservation methods are often insufficient, necessitating the development of innovative and natural approaches to improve the longevity and quality of these fruits. This study investigates the use of sericin, chitosan, and Aloe vera blended formulation (SBF) coating to extend the shelf life and quality of strawberries. The coated strawberries were stored at 14 ± 1 °C and 40–45% humidity, with their properties monitored over 12 days. Scanning electron microscopy (SEM) and fourier transform infrared (FTIR) spectroscopy analyses of strawberries confirmed the uniform application and chemical interaction of the coating, while contact angle measurements demonstrated improved wettability (contact angle 50.73 ± 0.6 °). The SBF coating significantly reduced weight loss and maintained higher firmness i.e. with only 7 ± 1.14% weight loss resulted after 9 days compared to a 16.5 ± 0.7% weight loss in uncoated strawberries. It also preserved higher levels of total soluble solids and titratable acidity compared to uncoated strawberries. Additionally, the SBF coating effectively slowed moisture loss, prevented infection, and regulated pH and respiration rate, demonstrating its potential for postharvest preservation of strawberries. [ABSTRACT FROM AUTHOR]

Lettuce is an easy-to-grow and nutrient-rich leafy vegetable. It grows well using a static hydroponic system, which saves space and is easy to maintain. However, understanding pH’s impact on lettuce growth in static hydroponic systems is limited. Hence, this study was conducted to determine the effect of pH nutrient solution on the growth performance and eating quality of lettuce grown in a static hydroponic system. Lettuce was grown in pH 5.2, 6.2, and 7.2 nutrient solutions. Its growth performance was collected weekly, including plant height, root length, number of leaves, leaf area, leaf chlorophyll content, total dry weight, and total moisture content. The harvested lettuce was analyzed for firmness, soluble solids concentration, titratable acidity, pH, and ascorbic acid content by the fourth week after transplanting. The plant height, root length, number of leaves, leaf area, and total dry weight of lettuce were affected by the interaction between nutrient solution pH and weeks after transplanting. By the third week after transplanting, lettuce grown in pH 6.2 was 11.12 and 18.67% taller than those grown in pH 7.2 and 5.2 nutrient solutions, respectively. By the fourth week after transplanting, the firmness of lettuce grown in pH 6.2 was significantly higher than those grown in pH 5.2 and 7.2 nutrient solutions by 2.34 and 7.32%, respectively. It is concluded that lettuce should be grown in a pH 6.2 nutrient solution when using a static hydroponic system. [ABSTRACT FROM AUTHOR]

Fruit firmness in sweet cherries (Prunus avium L.) is a critical quality parameter highly valued by consumers as it is associated with fruit freshness. In general, firm fruit also cope better with storage and handling. Gibberellic acid (GA) is commonly used by sweet cherry producers to increase firmness, soluble solids content and fruit size. This study evaluated the effects of GA on the rheological properties of sweet cherry fruit at harvest and postharvest storage. Specifically, GA's influence on susceptibility to mechanical damage during handling was evaluated. The following GA treatments were applied to two sweet cherry cultivars 'Bing' and 'Lapins': T0, control, T30—GA at 15 ppm applied at pit-hardening and straw-colour stages; T45—GA at 25 ppm at pit-hardening and GA at 20 ppm at straw-colour; and T60—GA at 30 ppm applied at pit-hardening and straw-colour. The results indicate that GA delayed harvest by two to four days in both cultivars, with 'Lapins' also showing a significant increase in fruit size. Regardless of spray concentration, GA increased the modulus of elasticity and fruit resistance evaluated as stress at the maximum point at harvest. These effects persisted after 35 days of storage at 0 °C and an additional three days of shelf-life at 15 °C. While the strain or deformation capacity of the fruit at bioyield at harvest was constant across treatments, it was, however, lower in the GA-treated fruit than in the controls during storage at 0 °C under the high-humidity conditions of modified atmosphere packaging. The less mature fruit harvested at colour 3.0 (red/mahogany) were stiffer (reduced deformation) and more sensitive to induced mechanical injury than the fruit harvested later at colour 3.5 (mahogany). The GA treatments increased fruit resistance to damage without increasing tissue deformability. Other questions associated with stiffer tissues and lower deformability during storage at 0 °C under high humidity should be further studied, specifically cultivars that are naturally high in box-cracking sensitivity during storage. [ABSTRACT FROM AUTHOR]

Cornelian cherry (Cornus mas L) fruit is very perishable and needs proper post-harvest management to reduce waste. This study was performed as a factorial experiment in a completely randomized design with three replications with the aim of increasing postharvest life and maintaining fruit quality of two Cornelian cherry genotypes KKP2 and Hir. Factors included three salicylic acid immersion treatments (0, 1 and 2 mM), four storage times (0, 10, 20 and 30 days) and two Cornelian cherry genotypes. Fruits were harvested at maturity (more than 90% redness of the skin) and healthy and uniform fruits were subjected to immersion treatments and stored for 30 days at 4 ° C. During and at the end of experiment, different physicochemical traits of fruits including soluble solids (TSS), firmness, total phenol, anthocyanin, ascorbic acid, pH and weight loss were examined at 10-day intervals. The results showed that KKP2 genotype had less firmness and TSS and more weight loss than Hir genotype. There was no significant difference between the two genotypes in terms of total acidity. The highest of pH, soluble solids and weight loss were observed in the control or distilled water treatment and salicylic acid treatment prevented the increase of these parameters. The highest levels of ascorbic acid, anthocyanin and firmness were observed in the treatment of 1 mM salicylic acid. The amount of fruit phenol in distilled water treatment was further reduced and the treatments of salicylic acid 1 and 2 mM prevented further reduction of phenol during storage. Salicylic acid treatments can be used as a promising method for increasing the firmness and extending the shelf life of cornelian cherry fruits. [ABSTRACT FROM AUTHOR]

The quality of amla fruits is greatly affected by post-harvest factors, especially storage temperature and time, which undergo rapid quality changes after harvest. Therefore, a detailed storage study was necessary for amla fruit. Hence, this study was conducted to determine the impact of different storage temperatures and durations on physicochemical, textural, bioactive, and microstructure properties of amla fruit. Storage analysis was performed at low (6 °C) and room temperature (25 °C) conditions. The results showed that fructose sugar significantly (p < 0.05) decreased by 15.79% during room temperature (RT) storage due to a higher respiration rate (14.32–45.98 mL kg− 1h− 1) in the RT environment, while it was non-significantly affected during low-temperature (LT) storage. Although, total soluble solid (TSS) of RT-stored amla increased by 76.67% due to moisture loss (7.82%). These results correlated that increased TSS is due to moisture loss, unrelated to ripening or starch degradation. In addition, total phenol content (TPC) of RT-amla was increased by 28.23%, while it slightly decreased by 6.58% for LT-amla. At the same time, variations in TSS, weight, and moisture were insignificant during LT storage, resulting in no significant changes in textural properties and SEM microstructures. Textural properties of RT and LT-stored amla exhibited opposite trends during storage, which can be correlated with reduced intercellular space and increased gumminess in SEM microstructure analysis. These findings provide new insights into governing amla fruit's characteristics during storage, which will be beneficial in enhancing shelf-life. [ABSTRACT FROM AUTHOR]

Predicting the post-storage quality of cherry fruits is crucial for determining their suitability for long-distance shipping or domestic distribution. This study aimed to forecast key quality attributes of Staccato sweet cherries after storage, simulating shipping conditions, by analyzing spring soil, leaf, fruitlet, and at-harvest data from thirty orchards in the Okanagan Valley, British Columbia, Canada, over two years. A support vector machine (SVM) was used to predict post-storage variables, with pre-harvest and at-harvest data selected by a genetic algorithm. The SVM accurately predicted soluble solids (R2 = 0.88), firmness (R2 = 0.83), and acidity (R2 = 0.79) after four weeks of storage, as well as visual disorders like slip skin and stem browning. Spring soil properties (Ca, Mg), leaf (N, Ca, Mg, Fe, Zn, B), and fruitlet data (N, Ca, Mg, B) were key predictors. Leaf Ca was vital for firmness and total soluble solids (TSS) prediction, while N in leaves and fruitlets influenced firmness, acidity, and disorders. Leaf Zn helped predict weight and acidity/TSS ratio, and Mg impacted fruit color. Pre-harvest leaf nutrition measured 3–4 weeks before harvest, proved most effective in predicting post-storage quality. [ABSTRACT FROM AUTHOR]

The physico-chemical properties of potato tubers change with age, which can affect not only the quality of the tubers but also the quality and shelf-life of fresh-cut potatoes (FCP) produced from older tubers. One of the methods that has proven useful for extending the shelf-life of some foods is ultraviolet-C (UV-C) irradiation. This study investigated whether UV-C irradiation can mitigate the negative effects of tuber age. FCP from tubers after storage (1 and 9 months), vacuum-packaged, were irradiated with UV-C (2.70 kJ m−2) and stored (6 °C/15 days). The total aerobic mesophilic bacteria count (TAMBC), total and soluble solids content, pH, firmness, color, chlorogenic acid, reducing sugars, sensory properties of raw FCP and subsequently boiled and fried FCP, and acrylamide content in fried FCP were monitored. UV-C irradiation of FCP does not affect all parameters equally for both tuber ages, but was generally effective in reducing TAMBC, and UV-C-treated samples reached a shelf-life of 15 days. UV-C irradiation improved the sensory properties of raw and thermally processed FCP at both ages. UV-C irradiation caused an increase in acrylamide content in fried FCP, regardless of. the tubers age, but all samples were safe in this respect. [ABSTRACT FROM AUTHOR]

Changes in both lignin biosynthesis and DNA methylation have been reported to be associated with chilling stress in plants. When stored at low temperatures, red-fleshed loquat is prone to lignification, with increased lignin content and fruit firmness, which has deleterious effects on taste and eating quality. Here, we found that 5 °C storage mitigated the increasing firmness and lignin content of red-fleshed 'Dahongpao' ('DHP') loquat fruit that occurred during 0 °C storage. EjNAC5 was identified by integrating RNA sequencing with whole-genome bisulfite sequencing analysis of 'DHP' loquat fruit. The transcript levels of EjNAC5 were positively correlated with changes in firmness and negatively correlated with changes in DNA methylation level of a differentially methylated region in the EjNAC5 promoter. In white-fleshed 'Baisha' ('BS') loquat fruit, which do not undergo chilling-induced lignification at 0 °C, the transcripts of EjNAC5 remained low and the methylation level of the differentially methylated region in the EjNAC5 promoter was higher, compared with 'DHP' loquat fruit. Transient overexpression of EjNAC5 in loquat fruit and stable overexpression in Arabidopsis and liverwort led to an increase in lignin content. Furthermore, EjNAC5 interacts with EjERF39 and EjHB1 and activates the transcription of Ej4CL1 and EjPRX12 genes involved in lignin biosynthesis. This regulatory network involves different transcription factors from those involved in the lignification pathway. Our study indicates that EjNAC5 promoter methylation modulates EjNAC5 transcript levels and identifies novel EjNAC5–EjERF39– Ej4CL1 and EjNAC5–EjHB1– EjPRX12 regulatory modules involved in chilling induced-lignification. [ABSTRACT FROM AUTHOR]

Background: Kiwiberry is an emerging edible fruit with market potential owing to its advantages of small size, thin and hairless skin, and sweet taste. However, kiwiberry is highly susceptible to softening after harvest, which poses a challenge for storage and transport. To reveal the underlying cause of kiwiberry softening, it is essential to investigate the characteristics of postharvest fruit and the molecular mechanisms that affect changes in fruit firmness. Results: Morphological observations and analysis of physical parameters showed that the skin of kiwiberry did not change markedly from the 1st to the 7th day after harvest, while the colour of the inner pericarp gradually turned yellow. By the 9th day of room temperature storage, the kiwiberries began to rot. The hardness decreased rapidly from the 1st to the 5th day postharvest, reaching the low level on the 5th day. The starch and pectin contents of kiwiberry showed a downward trend with increasing storage time. Transcriptome sequencing and weighted gene co-expression network analysis identified 29 key genes associated with the changes in the hardness of kiwiberry after harvest. Gene Ontology enrichment analysis indicated that these 29 genes are mainly involved in pectin metabolism, starch synthesis, starch decomposition, and starch metabolism. In addition, three transcription factors, AGL31, HAT14, and ALC, were identified to be strongly positively correlated with the 29 genes that affect the hardness changes of kiwiberry after harvest, and 28 of the 29 key genes were predicted to be regulated by HAT14. Conclusions: These results reveal the changes in morphological characteristics and physiological indicators during the postharvest ripening and softening of kiwiberry stored under room temperature conditions. Transcriptome analysis identified 29 key genes and three transcription factors that affect the firmness changes of postharvest kiwiberry. The results of this study thus provide insight into the transcriptional regulatory mechanism of kiwiberry softening during storage to improve the postharvest quality. [ABSTRACT FROM AUTHOR]

Parsnip, a root vegetable from the Apiaceae family, is rich in dietary fiber, pectin, and starch but remains relatively unfamiliar in South Korea. This study investigated the physicochemical properties of two Korean-grown parsnip cultivars, 'Warrior' and 'Albion', focusing on their organic acid and sugar compositions. The 'Warrior' cultivar has higher firmness and water content but lower SSC compared to 'Albion'. In 'Warrior', malic and lactic acids were the main organic acids, while 'Albion' had predominant oxalic and malic acids. Malic acid was also the primary organic acid in the inedible parts of 'Warrior', and oxalic acid in 'Albion'. In the edible parts of both cultivars, sucrose was identified as the main sugar. In 'Warrior', the levels were 88.59%, 90.35%, and 79.13% in the cortex, pith, and skin, respectively, while in 'Albion', the levels were 88.56%, 64.40%, and 67.39%. 'Warrior' showed higher total sugar content in its cortex (6.66%) compared to 'Albion' (3.67%). These results highlight the beneficial compounds in parsnips and suggest their potential for improving dietary strategies and health. [ABSTRACT FROM AUTHOR]

Xylella fastidiosa subsp. pauca (Xfp) is a significant threat to Mediterranean agriculture, particularly impacting olive trees in southern Italy, causing Olive Quick Decline Syndrome. Resistant olive cultivars, such as 'Leccino' and 'Fs-17', have been identified as alternatives to restore the oliviculture within the infected areas. 'Frantoio' and 'Cipressino' are included in ongoing studies on genetic resistance to Xfp. The mechanization of olive harvesting is essential for reducing production costs in the olive oil sector. Two systems, trunk shakers and over-the-row machines, are used depending on the tree density and canopy structure, with super-high-density systems offering advantages in terms of cost and efficiency. This study investigates the feasibility of using simple and non-destructive indices to assess the optimal mechanical harvesting time. Different physical ripening indices, including detachment force, fresh weight, pigmentation, and firmness, were measured on four olive cultivars ('Fs-17', 'Leccino', 'Frantoio', 'Cipressino') in southern Italy over two years. The study found that the pigmentation index had a strong relationship with the detachment index, particularly for 'Fs-17', and 'Leccino', providing a reliable non-destructive measure for optimal harvesting time. The results indicate that the optimal harvesting times for mechanical harvesting are early September for 'Cipressino', early October for 'Fs-17', and mid-October for 'Frantoio' and 'Leccino'. [ABSTRACT FROM AUTHOR]

The effect of N-(2-chloro-4-pyridyl)-N′-phenylurea (CPPU) on fruit splitting of 'Murcott' tangor fruit (Citrus reticulate × Citrus sinensis) was analyzed throughout two successive seasons during 2020 and 2021. 'Murcott' trees were treated with various concentrations of CPPU, 1.0, 5.0, and 10.0 mg·L−1, which significantly reduced the splitting of fruit compared with the control fruit and the gibberellic acid (GA3)-treated fruit (20.0 mg·L−1). The fruit treated with 5.0 mg·L−1 CPPU exhibited higher levels of fruit weight, longitudinal diameter, transverse diameter, peel strength, and peel thickness compared to the control fruit. In addition, the tree sprayed with 5.0 mg·L−1 CPPU had the greatest number of cellular layers and cells at the stylar end calculated in fruit. However, there were no other fruit quality characteristics significantly affected by CPPU and GA3 treatments. [ABSTRACT FROM AUTHOR]

Understanding the fundamental light-sample interaction process is a crucial step toward the development of vibrational spectroscopy to determine fruit texture (i.e., firmness). This study aimed to investigate the effect of pectin constitution, including total pectin, water-soluble pectin, protopectin contents, and protopectin index (PI), on the optical properties and firmness of 'Baifeng' and 'Xiahui 8' peach flesh at the different softening degrees during postharvest storage of 6 days at 20 °C. The firmness of 'Baifeng' and 'Xiahui 8' peaches significantly (p < 0.05) changed with a decreasing rate from 90.3% to 92.2%. Peach firmness of these two cultivars correlated well with PI contents (r > 0.912) and showed good internal correlations with optical scattering properties. The light absorption coefficient (μa) and reduced scattering coefficient (μ's) at 600–1600 nm were measured using a single integrating sphere system combined with an inversion algorithm. This relationship of μa and μ's with peach firmness and pectin constitution was first analyzed. Notably, the specific μ's at 660 nm, 950 nm, 1203 nm, and 1453 nm showed a satisfactory prediction of peach firmness and PI of 'Xiahui 8' (R2 ≥ 0.926) and 'Baifeng' peaches (R2 ≥ 0.764), respectively. Furthermore, the prediction models were established based on partial least squares regression coupled with optical properties, and considerable prediction performances were obtained for tissue firmness (Rp2 ≥ 0.863) and PI based on μ's (Rp2 ≥ 0.802). Consequently, these results further verified that the spectroscopic prediction model for peach firmness could be related to the high correlations between PI in tissues and their optical scattering properties. Future research interests could include the development of optical absorption and scattering sensors for rapid and efficient determination of peach firmness. [ABSTRACT FROM AUTHOR]

Indian jujubes are perishable fruits with a short storage life after harvest. In this study, Indian jujube fruits were stored at 5 °C and 90% RH for 30 days after treating the fruits with 1% chitosan (CHS), 1 mM putrescine (PUT), and a combination of 1% CHS and 1 mM PUT (CHS+ PUT). Physicochemical characteristics and cell wall degrading enzymes were measured at 10-day intervals. The results indicated that the efficiency of the CHS+PUT was better than CHS or PUT as individual treatments. The CHS+PUT treatment substantially decreased weight loss, decay incidence, and malondialdehyde content in Indian jujube fruits. The CHS+PUT treatment minimized losses of bioactive compounds (ascorbic acid and phenols), total antioxidant activity, and titratable acidity. In addition, CHS+PUT treatment delayed fruit softening by suppressing polygalacturonase activity and pectin methylesterase enzymes. Soluble solids content significantly increased through storage time, but applying CHS and PUT individually or in combination inhibited this increase. These results confirmed that the 1% CHS and 1 mM PUT treatments maintained the postharvest quality of Indian jujube fruits in cold storage. [ABSTRACT FROM AUTHOR]

The effects of 1% biofilm (ParkaTM) treatment regimes, initiated three (biofilm-3), two (biofilm-2), and one week (biofilm-1) prior to the commercial harvest, on fruit quality traits during the postharvest storage stage were investigated in this study. The treated fruit were stored at 0 ± 0.5°C and 90 ± 5% RH for 21 days. The application of biofilm treatments had no discernible effect on weight loss. In fact, biofilm-1 was found to have the opposite effect, resulting in increased weight loss. Respiration rates of biofilm-treated fruit were generally higher than those of control. Firmness of the biofilm treatments was higher than that of the control, both at harvest and during storage. Red coloration of the fruit was delayed by the biofilm treatments. Biofilm treatments caused a significant increase in the SSC of the fruit. The acidity of the control was lower than that of the biofilm treatments from the time of harvest until the 14th d. At the end of 21 d, there was no significant difference in acidity between the treatments. The effects of the treatments on vitamin C differed according to the date of measurement. At the end of storage, vitamin C was higher in fruit treated with biofilm-1 and biofilm-2. All biofilm treatments caused a significant increase in total phenolics, total flavonoids, anthocyanin content and antioxidant activity of the fruit, which was more evident in the biofilm-1 treatment. The results showed that biofilm treatments were more effective in maintaining fruit firmness and bioactive compounds during storage.

Abstract Background Kiwiberry is an emerging edible fruit with market potential owing to its advantages of small size, thin and hairless skin, and sweet taste. However, kiwiberry is highly susceptible to softening after harvest, which poses a challenge for storage and transport. To reveal the underlying cause of kiwiberry softening, it is essential to investigate the characteristics of postharvest fruit and the molecular mechanisms that affect changes in fruit firmness. Results Morphological observations and analysis of physical parameters showed that the skin of kiwiberry did not change markedly from the 1st to the 7th day after harvest, while the colour of the inner pericarp gradually turned yellow. By the 9th day of room temperature storage, the kiwiberries began to rot. The hardness decreased rapidly from the 1st to the 5th day postharvest, reaching the low level on the 5th day. The starch and pectin contents of kiwiberry showed a downward trend with increasing storage time. Transcriptome sequencing and weighted gene co-expression network analysis identified 29 key genes associated with the changes in the hardness of kiwiberry after harvest. Gene Ontology enrichment analysis indicated that these 29 genes are mainly involved in pectin metabolism, starch synthesis, starch decomposition, and starch metabolism. In addition, three transcription factors, AGL31, HAT14, and ALC, were identified to be strongly positively correlated with the 29 genes that affect the hardness changes of kiwiberry after harvest, and 28 of the 29 key genes were predicted to be regulated by HAT14. Conclusions These results reveal the changes in morphological characteristics and physiological indicators during the postharvest ripening and softening of kiwiberry stored under room temperature conditions. Transcriptome analysis identified 29 key genes and three transcription factors that affect the firmness changes of postharvest kiwiberry. The results of this study thus provide insight into the transcriptional regulatory mechanism of kiwiberry softening during storage to improve the postharvest quality.

Abstract Background The changes in the physical structures of the products are the first things that consumers pay attention to. Therefore, it is essential and significant importance to take measures to improve the storage conditions of products and to minimize quality losses. The main objective of the study was to evaluate the effects of agro-ecological conditions on bioactive compounds and fruit quality of kiwifruit during cold storage. The ‘Hayward’ kiwifruit cultivar grown in Ordu, Giresun, Samsun, Rize, and Yalova provinces of Türkiye were kept at 0 ± 0.5 °C and relative humidity of 90 ± 5% for 150 d. Results The kiwifruit obtained from the provinces of Yalova, Ordu, and Giresun experienced the least weight loss during cold storage. Kiwifruit from Samsun and Yalova provinces had the lowest fruit firmness, while those from Giresun had the highest on 150th d. The changes were observed in the skin and flesh colors of the kiwifruit belonging to all cultivation areas. The amount of vitamin C increased throughout the study in all ecological conditions, but the Yalova province’s kiwifruit was found to have the highest levels. Additionally, in all ecologies, kiwifruit showed an increase in antioxidant activity, total phenolics, and total flavonoids, all known to have beneficial effects on human health. The total antioxidant activity and total phenolics were highest in the kiwifruit of Yalova province, but the total flavonoids were found in the kiwifruit of Rize and Ordu provinces. Conclusion The study’s results revealed that kiwifruit’s bioactive compounds and quality parameters may vary depending on the cultivation area. Additionally, it can be stated that Yalova province kiwifruit experiences the least amount of postharvest quality losses.

Apricots being highly perishable and often results in significant postharvest losses that affect their marketability and commercial viability. A study was conducted to investigate the synergistic effect of postharvest application of gamma irradiation and Methyl Jasmonate (MeJ) on the quality, enzyme-specific activities, and shelf life of the apricot (Prunus armeniaca cv. ‘CITH-1’). Apricots were gamma irradiated at a dosage of 0.5 kGy and submerged in different concentrations of MeJ before being refrigerated (1±1 °C, RH 80-85%) for 35 days. Apricots treated with a 0.5 kGy dose and 0.1 mmol L-1 MeJ followed by refrigeration significantly (P<0.05) retained higher levels of antioxidant activity. The quality of apricots was effectively preserved, and their shelf life was extended through treatments of 0.5 kGy irradiation dosage and 0.2 mmol L-1 MeJ, which significantly (P<0.05) reduced decay percentage, weight loss, and enzyme activity while maintaining fruit firmness. The study's results suggest that applying a postharvest treatment of MeJ at a concentration of 0.1 and 0.2 mmol L-1 in combination with an irradiation dosage of 0.5 kGy is a viable method for preserving the quality and bioactive compounds of fresh apricots during refrigerated storage for up to 35 days. [ABSTRACT FROM AUTHOR]

Blueberry (Vaccinium spp.) is among the most-consumed soft fruit and has been recognized as an important source of health-promoting compounds. Highly perishable and susceptible to rapid spoilage due to fruit softening and decay during postharvest storage, modern breeding programs are looking to maximize the quality and extend the market life of fresh blueberries. However, it is uncertain how genetically controlled postharvest quality traits are in blueberries. This study aimed to investigate the prediction ability and the genetic basis of the main fruit quality traits affected during blueberry postharvest to create breeding strategies for developing cultivars with an extended shelf life. To achieve this goal, we carried out target genotyping in a breeding population of 588 individuals and evaluated several fruit quality traits after 1 day, 1 week, 3 weeks, and 7 weeks of postharvest storage at 1°C. Using longitudinal genome-based methods, we estimated genetic parameters and predicted unobserved phenotypes. Our results showed large diversity, moderate heritability, and consistent predictive accuracies along the postharvest storage for most of the traits. Regarding the fruit quality, firmness showed the largest variation during postharvest storage, with a surprising number of genotypes maintaining or increasing their firmness, even after 7 weeks of cold storage. Our results suggest that we can effectively improve the blueberry postharvest quality through breeding and use genomic prediction to maximize the genetic gains in the long term. We also emphasize the potential of using longitudinal genomic prediction models to predict the fruit quality at extended postharvest periods by integrating known phenotypic data from harvest. [ABSTRACT FROM AUTHOR]

Textural deterioration in frozen vegetables is a result of softening after freeze-thawing and is a critical issue to address. In the present study, field experiments were conducted on broccoli using six cultivars of three sizes (i.e., different maturities) in two seasons (spring and winter) to evaluate the relationship between the level of tissue softening caused by the freezing process and composition of pectin, which is a main component of the cell wall. The mechanical properties after freeze-thawing depended more on the growing season than on the cultivar or maturity, i.e., the maximum stress after freeze-thawing of spring broccoli was higher than that of winter broccoli. Between the winter broccoli, the samples harvested at lower temperatures showed more pronounced softening after thawing. For all broccolis, the temperature during harvest was strongly correlated with the maximum compressive stress of frozen broccoli (R = 0.867). Broccoli harvested in winter had a higher ratio of galacturonic acid in water-soluble pectin than that harvested in spring, indicating weakened cell wall adhesion. However, the absence of a substantial correlation between the pectin content and mechanical properties of broccoli, suggests that other factors such as cold-responsive materials are also involved in the tissue softening of frozen broccoli. [ABSTRACT FROM AUTHOR]

During kiwifruit storage, quality monitoring is required for inventory planning and consistent quality maintenance. Commercial near-infrared (NIR) spectrophotometers showed reduced performance in the estimation of kiwifruit flesh firmness (FF) as the FF estimation is indirect and can be affected when both, textural structures and absorbing compounds, change during postharvest ripening. Laser backscattering imaging (LBI) records the backscattered signal after a single laser beam interacts with kiwifruit tissue, including merged information on light absorption and scattering. Measurements were carried out at 830 nm, where scattering is most dominant. In this work, time series of kiwifruit 'Zesy002' (n = 30) and 'Hayward' (n = 30) LBI were collected through the postharvest ripening during a 15-day shelf life at 20°C. Four LBI parameters capturing DIP, FWHM, SLP and HWQM were selected in this study. "Zesy002' DIP, FWHM, SLP, and HWQM increased approx. 0.6 cm, 0.2 cm, 0.3 and 0.14 cm, respectively. 'Hayward' LBI increased approx. 0.2 cm, 0.1 cm, 0.2 and 0.04 cm, respectively. Different initial LBI values between cultivars and LBI changes may reflect the actual stage of softening, affected by kiwifruit ripeness. In conclusion, time series analysis could be useful in describing kiwifruit LBI change during ripening and making forecasts. [ABSTRACT FROM AUTHOR]

Postharvest assessment of 'Hass' avocado fruit ripening is frequently monitored by the fruit texture as it changes from hard to soft and 'buttery'. This large change in texture is easily measured, commonly by a penetrometer after removal of a small area of skin or by a whole-fruit compression measurement. The penetrometer is slow and destructive whereas the compression measurement is rapid and may be considered as non-destructive depending on the method. Investigation of the two methods on the same batch of fruit reveals information pertinent to the understanding of fruit ripening — beyond the simple provision of a numerical assessment of the progress of ripening. Monitoring of fruit under drying conditions revealed the role that fruit turgor plays in the compression measurement, and how it contributes to our understanding of the numerical values. Likewise, the observed variation among individual fruit ripening times may mean that averaged values are misleading, suggesting that fruit ripening takes longer than it actually does. The key element in the fruit-to-fruit variability was the pre-climacteric period before the fruit ripened. Additional penetrometer measurements of five regions of the fruit flesh for each fruit assessed quantified differences in mesocarp softening within the fruit. [ABSTRACT FROM AUTHOR]

Maintaining kiwifruit firmness is key to global supply chain. Controlled atmosphere (CA) technology can maintain kiwifruit quality. However, there is a risk of ethylene (C2H4) accumulation in CA that may accelerate kiwifruit softening. The objectives of this study were to determine the impact of ethylene on kiwifruit quality in CA. 'Hayward' kiwifruit were stored in air and CA (5% CO2 + 2% O2) at 0°C, 95% RH for 13 weeks. Ethylene at concentrations of 10, 100, 1000 nL·L−1 was added after 3 weeks of storage. The responses of kiwifruit to ethylene were dose-dependent in both air and CA storage. Ethylene-induced kiwifruit softening was delayed and slowed by CA. However, white-core inclusions (WCI) disorder was observed after 8 weeks of exposure to 100 and 1000 nL·L−1 ethylene in CA. The lowest concentrations of ethylene (≤ 10 nL·L−1) in CA did not influence kiwifruit quality. This work demonstrates that kiwifruit sensitivity to ethylene is lower in CA than that in air, but excessive softening and WCI may negatively impact kiwifruit quality when exposed to a high ethylene concentration in CA for extended periods. Hence, ethylene monitoring and management may be less critical in kiwifruit stored in CA but are required. [ABSTRACT FROM AUTHOR]

Textural softening is a major factor that limits the storage potential of fruit. Fresh produce markets incur severe financial losses due to excessive fruit softening. The application of preservation strategies aimed at mitigating fruit softening is crucial for optimising the marketability of fruit. Proposed preservation strategies include ecofriendly treatments, namely, hexanal, edible coatings, heat treatments, ozone and UV-C irradiation. These treatments optimise firmness retention by targeting the factors that affect fruit softening, such as ethylene, respiration rates, enzymes and pathogens. This review discusses the mechanisms by which ecofriendly treatments inhibit fruit softening, providing insights into their effect on ethylene biosynthesis, cell wall metabolism and disease resistance. Although ecofriendly treatments offer a promising and sustainable approach for delaying fruit softening, the optimisation of treatment application protocols is needed to improve their efficacy in retaining fruit firmness. Studies reporting on the molecular mechanisms by which ecofriendly treatments inhibit fruit softening are limited. Future studies should prioritise proteomic and transcriptome analyses to advance our understanding of the underlying molecular mechanisms by which ecofriendly treatments delay the fruit-softening process. [ABSTRACT FROM AUTHOR]

Background: The changes in the physical structures of the products are the first things that consumers pay attention to. Therefore, it is essential and significant importance to take measures to improve the storage conditions of products and to minimize quality losses. The main objective of the study was to evaluate the effects of agro-ecological conditions on bioactive compounds and fruit quality of kiwifruit during cold storage. The 'Hayward' kiwifruit cultivar grown in Ordu, Giresun, Samsun, Rize, and Yalova provinces of Türkiye were kept at 0 ± 0.5 °C and relative humidity of 90 ± 5% for 150 d. Results: The kiwifruit obtained from the provinces of Yalova, Ordu, and Giresun experienced the least weight loss during cold storage. Kiwifruit from Samsun and Yalova provinces had the lowest fruit firmness, while those from Giresun had the highest on 150th d. The changes were observed in the skin and flesh colors of the kiwifruit belonging to all cultivation areas. The amount of vitamin C increased throughout the study in all ecological conditions, but the Yalova province's kiwifruit was found to have the highest levels. Additionally, in all ecologies, kiwifruit showed an increase in antioxidant activity, total phenolics, and total flavonoids, all known to have beneficial effects on human health. The total antioxidant activity and total phenolics were highest in the kiwifruit of Yalova province, but the total flavonoids were found in the kiwifruit of Rize and Ordu provinces. Conclusion: The study's results revealed that kiwifruit's bioactive compounds and quality parameters may vary depending on the cultivation area. Additionally, it can be stated that Yalova province kiwifruit experiences the least amount of postharvest quality losses. [ABSTRACT FROM AUTHOR]

Selenium (Se) is a nutrient whose daily intake is often below the recommended levels in people. Biofortification with Se is a method to increase this intake by raising the Se concentration in tomato fruits, an effect dependent on sources and modes of application. Additionally, Se application can promote the enhancement of other compounds in tomato fruits, altering their metabolism, which may increase the fruit's shelf life. This study aimed to determine how different strategies of applying a multi-nutrient fertilizer containing Se (SeMNF) can increase the Se content and other bioactive compounds and enhance the shelf life of tomato (Solanum lycopersicum L.) fruits. Different foliar fertilization strategies involving the use of SeMNF were evaluated in field trials conducted on commercial tomato crops. Indeterminate-growth tomatoes were used, and different Se doses and application strategies were tested. Harvesting was conducted in three phases according to fruit ripening. Each harvested fruit was assessed for the Se content, macro and micronutrients, total phenolic compounds, vitamin C, antioxidant activity, carotenoids, pH, total titratable acidity, and total soluble solids in tomato fruits. Doses of 15 g ha−1 of Se, split into three applications, increased the Se content in the fruits at 1 and 2 harvests. The application of SeMNF at Se doses above 10 g of Se ha−1 increased firmness, days of ripening, and the nutritional quality of the tomatoes (higher contents of carotenoids (+39%), lycopene (+33%), antioxidant activity (+16%), total phenolic compounds (+38%), and vitamin C (+14%) in a dose-dependent effect of the application strategy used. These results contributed to an increase in the shelf life of tomatoes, consequently reducing food waste. [ABSTRACT FROM AUTHOR]

Understanding consumers' preferences for fruit quality attributes is key to informing breeding efforts, meeting consumer preferences, and promoting increased market demand. The objective of this study was to assess the effect of fruit quality traits and hedonic sensory evaluation on consumers' willingness to pay (WTP) for a selection of fresh northern and southern highbush blueberry cultivars. The WTP was elicited by using a double-bounded contingent valuation conducted in conjunction with a consumer sensory test. Two types of models were estimated using either sensory evaluations (i.e., consumer preference and consumer intensity) or instrumental measurement data (i.e., measures of soluble solids, titratable acidity, sugars, acids, and firmness) as explanatory variables to model WTP. Results using sensory evaluations indicated that flavor liking, flavor intensity, and sweetness intensity are key factors that influence consumers' acceptance and WTP for blueberries. A regression analysis using instrumental measurements indicated that measures related to sweetness and acidity traits are important factors that determine WTP. Higher WTP was associated with higher total sugar content across different levels of total organic acid. The WTP increases with organic acid content, because this is needed for enhanced flavor; however, WTP declines at high concentrations of organic acid. Except for extreme values of firmness, the WTP increased as measures of fruit firmness increased, indicating a consumer preference for firmer blueberries. Overall, the results provided new insights into the relationships between consumer preference and WTP and fruit quality benchmarks to select for improved quality. [ABSTRACT FROM AUTHOR]

Two eggplant cultivars (Brigitte and Dalong) were stored under ambient conditions for 8 days to examine the postharvest quality and shelf life. Results indicated that the respiration rate, firmness and springiness, and nutritional quality of both eggplant cultivars decreased with the extension of shelf life. On the contrary, opposite trends were observed in weight loss, gumminess, and chewiness of eggplant fruits. In addition, the weight loss of 'Brigitte' eggplant fruits was 3.3% and 6.9% lower compared with 'Dalong' eggplant fruits at 4 and 8 days after storage. Thicknesses of epidermal cells and the stratum corneum, the epicuticular wax content of 'Brigitte eggplant fruits increased by 42.9%, 766.7%, and 58.8% compared with 'Dalong' eggplant fruits, respectively, with a concomitant increase in the dense wax layer structure. In conclusion, the storage tolerance of 'Brigitte' eggplant fruits was higher than that of 'Dalong' eggplant fruits due to the higher epicuticular wax content and dense wax layer structure. [ABSTRACT FROM AUTHOR]

Tomatoes are delicate and prone to damage quickly, which ultimately leads to lower quality and increased post-harvest losses. Hence, an ideal storage environment is very important to maintain the quality of tomato fruits after harvest. Therefore, this study was conducted to determine the effect of storage conditions on the quality parameters of tomato fruits. Experiments were de-signed for six storage periods (4, 8, 12, 16, 20, and 24 days) and two temperatures (12 °C and room air temperature "22 °C"). Three tomato fruit quality parameters (Brix, color, and firmness) have been selected and measured for three tomato varieties (Ghandowra-F1, Forester-F1, and Feisty-Red) grown hydroponically at three salinity levels (2.5, 6.0, and 9.5 dS m−1) of the nutrient solution. Results showed that the highest mean Brix values, for all varieties, were recorded at the highest salinity (9.5 dS m−1), and were significantly (Pr < 0.0001) higher than those at medium (6.0 dS m−1) and low (2.5 dS m−1) salinity levels. In addition, the highest fruit firmness was recorded at high salinity level (9.5 dS m−1), but there was no significant difference to that recorded at medium salinity (6.0 dS m−1). Regarding tomato fruit color, the highest average values were recorded for the Ghandowra-F1 (2.51) and Forester-F1 (2.69) varieties at medium salinity (6.0 dS m−1), while the highest average color value for the Feisty-Red variety (1.54) was obtained at high salinity (9.5 dS m−1). On the other hand, the Brix, color, and firmness of tomato fruits were significantly affected by the storage temperature. Moreover, the mean Brix values (7.66%) were slightly higher at 12 °C storage temperature compared to those at 22 °C (7.38%). In general, the fruit color values gradually increased with the storage period, especially under 22 °C storage temperature, with peak color values of 2.73, 2.70, and 1.66 recorded on the 12th day of the storage period for Ghandowra-F1, Forester-F1, and Feisty-Red, respectively. Tomato fruit firmness decreased faster with the storage period at 22 °C compared to the storage temperature of 12 °C. However, the highest average values of fruit firmness for Ghandowra-F1 (9.37 N cm−1) and Forester-F1 (9.41 N cm−1) recorded at control condition were not significantly different those recorded on the 8th day of storage at 12 °C storage temperature. By contrast, the highest average value of fruit firmness for Feisty-Red (8.85 N cm−1) recorded at control condition was not significant than that recorded on the 4th day of the storage period at 12 °C storage temperature (8.82 N cm−1). Overall, tomato fruits can be stored at 12 °C temperature for up to 20–24 days, without negative effects on fruit quality. [ABSTRACT FROM AUTHOR]

The effects of normal (NA) and controlled atmosphere (CA) storage and postharvest treatment with 1-methylcyclopropene (1-MCP) before CA storage for 5 months on the volatilome, biochemical composition and quality of ‘Golden Delicious’ (GD) and ‘Red Delicious’ (RD) apples were studied. Apples stored under NA and CA maintained and 1-MCP treatment increased firmness in both cultivars. NA storage resulted in a decrease of glucose, sucrose and fructose levels in both cultivars. When compared to CA storage, 1-MCP treatment caused a more significant decrease in sucrose levels and an increase in glucose levels. Additionally, 1-MCP-treated apples exhibited a significant decrease in malic acid content for both cultivars. All storage conditions led to significant changes in the abundance and composition of the volatilome in both cultivars. GD and RD apples responded differently to 1-MCP treatment compared to CA storage; higher abundance of hexanoate esters and (E,E)-α-farnesene was observed in RD apples treated with 1-MCP. While 1-MCP was effective in reducing (E,E)-α-farnesene abundance in GD apples, its impact on RD apples was more limited. However, for both cultivars, all storage conditions resulted in lower levels of 2-methylbutyl acetate, butyl acetate and hexyl acetate. The effectiveness of 1-MCP is cultivar dependent, with GD showing better results than RD. [ABSTRACT FROM AUTHOR]

To assess the influence of 1-(3-phenyl-propyl) cyclopropene (1-PPCP) and salicylic acid (SA) on the storability of peach (cv. 'Early Swelling'), we applied the following treatments; 5 μL L−1 1-PPCP for 24 h, 2 mmol L−1 SA for 5 min, and a combination of both treatments, concurrently, a control group. The fruit samples were stored at 6 ± 1°C with 90–95% RH for 28 d, followed by 3 d at 20°C to simulate market shelf life. The objective was to maintain fruit quality and enhance fruit storability. 1-PPCP+SA significantly reduced decay, weight loss, and respiration rate, while also contributing to the maintenance of fruit colouration and SSC. The treatments also led to a decrease in browning incidence, malondialdehyde levels, and electrolyte leakage. Additionally, they effectively slowed down the activity of polyphenol oxidase (PPO). Furthermore, all treatments led to a reduction in ethylene production and the activity of key enzymes involved in ethylene biosynthesis, such as ACC-synthase and ACC-oxidase, when compared to the control group. Additionally, these treatments regulated related pectin-degrading enzymes such as pectin methylesterase (PME), β-galactosidase (β-Gal), and polygalacturonase (PG). Therefore, the utilisation of 1-PPCP and SA is suggested as post-harvest treatments to enhance the storability of peach while preserving overall quality. [ABSTRACT FROM AUTHOR]

This study evaluated the effect of salicylic acid (SA) application on yield, fruit quality, and post-harvest storage in Prunus avium subjected to deficit irrigation (DI). A field experiment with six-year-old P. avium cv. Lapins was performed under two water treatments: irrigation at 100% of crop evapotranspiration (ETc) [full irrigation (FI)] and irrigation at 60% ETc from the second fruit phase to harvest time (DI). A single 0.5 mM SA was applied to both water treatments at fruit color change. At harvest time, fruits were collected to determine yield, fruit quality, and quality during post-harvest storage (0, 10, 20, and 30 days). The DI reduced fruit yield (11%), fruit weight (8%), and caliber (6%) and increased firmness (7%) and total soluble solids (TSS) (5%) in P. avium compared with FI plants at harvest time. Our study showed that SA application recovered fruit yield (9%), fruit weight (5%), and caliber (4%), improving TSS in DI plants at day 0. Interestingly, SA application significantly reduced P. avium fruit cracking (78% in FI and 82% in DI). Fruit weight was reduced in all treatments, mainly decreasing by 14% in FI and 13% in DI plants at day 30 of post-harvest storage. Fruit weight did not change during post-harvest storage with SA, except on day 30, where a slight reduction was observed. TSS showed no significant differences during post-harvest storage for all treatments. Therefore, SA could be an interesting tool to mitigate the impact of DI on the yield and fruit quality of P. avium and to reduce fruit cracking and prolong fruit quality during post-harvest storage. [ABSTRACT FROM AUTHOR]

With the objective of shipping squash fruit during the winter season, which constitutes the off-season for domestically grown squash fruit, we aimed to develop storage techniques that mitigate the softening of squash fruit mesocarp that typically occurs during prolonged storage. Utilizing near-infrared spectroscopic analysis to sort squash fruit based on dry matter immediately after harvest proved to be efficacious in limiting mesocarp softening. Through this approach, fruits with a dry matter ratio of 30 % (w/w) or higher, when stored at 10 °C, demonstrated the ability to maintain their mealy texture even after a 2-month storage period. Furthermore, employing a highly mealy cultivar and storing it at a temperature of approximately 10 °C enabled the preservation of the mealy texture for over 2 months, eliminating the necessity for screening based on dry matter. [ABSTRACT FROM AUTHOR]

The task of achieving global food security has become progressively more critical, with widespread hunger and a lack of access to adequate and nutritious food supplies. The agricultural industry including fruits and vegetable production plays a pivotal role in this endeavor. However, fruits and vegetables are perishable products which losses their quality within short period of time, unless their shelf life is extended by applying different processing techniques. Processing and the inherent properties of fruits and vegetables significantly impact their quality, shelf life, and the optimal processing system employed. Therefore, this paper aims to provide a comprehensive review of quality and phsio-chemical Changes associated with processing and the diverse properties of fruits and vegetables.

Tomatoes are a globally significant vegetable valued for their nutritional benefits and culinary versatility. However, they are prone to mechanical damage, such as dropping and bruising, during harvesting and handling, which can impair their physicochemical and nutritional properties, reduce shelf life and marketability. This study aimed to evaluate the effects of dropping and bruising on the physicochemical and nutritional properties of tomatoes. Vanessa F1 Hybrid tomatoes at two maturity stages, Breaker (II) and Red ripe (VI) were dropped from a height of 90 cm and stored at various temperatures for 15 days. We assessed changes in firmness, total soluble solids (TSS), titratable acidity, respiration rate, vitamin C, and mineral contents (K and Ca) over the storage period. Dropped tomatoes exhibited significantly increased internal bruising and accelerated deterioration over time, with Red ripe tomatoes being more affected. Firmness, TSS, titratable acidity, and vitamin C content significantly decreased, while respiration rates and mineral content also reduced due to dropping. Breaker stage tomatoes showed better retention of firmness and nutrient content compared to Red ripe stage tomatoes, particularly when stored at 5 °C. Mechanical damage from dropping significantly degrades the quality of tomatoes, affecting their physical and chemical properties. The study highlights the importance of minimizing mechanical injuries to maintain tomato quality and extend shelf life. This knowledge can aid in developing improved postharvest handling practices to enhance the competitiveness and sustainability of the tomato industry.

Two field experiments were carried out in 2022 and 2023 within commercial watermelon fields in Stockton and Modesto, CA, USA. Two Trichoderma-containing products were applied to the grafted and nongrafted watermelon seedlings through tray soaking or field chemigation. All seedlings were mechanically transplanted into a split-split plot design with the Trichoderma product as the main factor and application method (tray soaking and chemigation) as the sub-plot. The sub-sub-factor included three interspecific hybrid squash rootstocks (Cucurbita maxima × Cucurbita moschata) ‘Cobalt’, ‘Flexifort’, and ‘RS841’ that were grafted with the commercial seedless watermelon scion ‘Summer Breeze’. All treatments were replicated four times. Vine health was visually assessed three times, and canopy coverage was assessed for a total of six measurements for each year. Harvest was conducted three times in 2022 and twice in 2023 to analyze yield and quality differences among treatments. Aboveground and root samples from defective plants were taken amid the harvest and shipped to the University of California, Davis Fungal Pathology Diagnostics Research Laboratory for identification of soilborne fungal pathogens to confirm or rule out their involvement in vine declines initially attributed to nonpathogenic factors. The laboratory diagnosis indicated that Macrophomina was morphologically identified from the foot and root isolations of 67% of the submitted nongrafted, non-Trichoderma-biofungicide–treated plants in 2022. Further sequencing results confirmed the primary pathogen, Macrophomina phaseolina, from 50% of those submitted plant samples. In addition, putative Fusarium root and stem rot (Fusarium oxysporum f. sp. radices-cucurmerinum) and Falciforme crown rot and decline (Fusarium noneumartii) from runners and roots of nongrafted, noninoculated plants in the 2022 experiment were also reported. However, no significant soilborne fungal pathogens were found in the 2023 experiment. In both trials, the effects of Trichoderma-containing biofungicides and their application methods on preventing vine decline, maintaining canopy coverage, and enhancing fruit yield and quality were not as remarkable as grafting. The average grafting effect contributed to 83% and 53% decrease of vine decline compared with the nongrafted plants in 2022 and 2023. Overall, grafted plants yielded 47.6% and 32.4% more than the nongrafted counterpart in 2022 and 2023, respectively. In addition, grafting exerted predominant influence on fruit firmness and rind thickness. The study results indicated that grafting onto multipathogen resistance watermelon rootstocks serves as an effective production tool to maintain fruit yield, quality, and plant health under both pathogenic and disease-free conditions. Further work is still needed to continue evaluating best practical application protocols of Trichoderma-based biofungicides and other biopesticides to enhance product effectiveness and end users’ confidence in reducing soilborne diseases and reliance on conventional fumigants.

South African 'Hass' avocado fruit harvested early season are vulnerable to colour desynchronisation with softening during ripening, reducing their international and local market aesthetic value. So far, research has proven that 'Hass' avocado fruit exocarp colour desynchronization is an early season conundrum. However, there is insufficient literature on underlying factors causing exocarp colour desynchronisation. Therefore, the study aimed to investigate the ripening temperature effect on early season 'Hass' avocado fruit exocarp colour development and pigments during ripening. Early season 'Hass' avocado fruit were stored at 5.5 °C for 28 days thereafter, ripened at 16, 21 and 25 °C and evaluated for firmness, visual and objective colour (L*, C* and h°), total chlorophyll and anthocyanin content. The results showed that the visual colour of fruit ripened at 25 °C was significantly higher compared to 21 and 16 °C after 2 and 4 ripening days. This study found that ripening temperature had no significant influence on chlorophyll degradation. However, fruit ripened at 25 °C accumulated significantly higher exocarp anthocyanin concentration compared to lower ripening temperatures. In conclusion, ripening early season 'Hass' avocado fruit at 25 °C resulted in improved exocarp colour development and anthocyanin accumulation.

Abstract Background Fruit cracking impacts the quality of sweet cherry, significantly affecting its marketability due to increased susceptibility to injury, aesthetic flaws, and susceptibility to pathogens. The effect of 1% biofilm (Parka™) application regimes on fruit cracking and other quality parameters in the ‘0900 Ziraat’ cherry cultivar was investigated in this study. Fruit sprayed with water were served as control (U1). Fruit treated only once with biofilm three, two and one week before the commercial harvest were considered as U2, U3 and U4, respectively. Fruit treated with biofilm three, two, and one week before harvest were considered as U5; three and two week before harvest as U6; two and one week before harvest as U7; and fruit treated three and one week before harvest as U8. Results In both measurement periods, the lower cracking index was obtained in biofilm-treated sweet cherry fruit. However, the firmness of biofilm-treated fruit was higher than that of the control fruit. The lowest respiration rate was observed in U7, while the highest weight was recorded in U4 and U5 than the control. The biofilm application decreased fruit coloration. The biofilm application also increased the soluble solids content of the fruit. The U2, U3 and U4 applications at harvest showed higher titratable acidity than the control. In both measurement periods, the vitamin C content of the U2, U5, U6, U7 and U8 applications was found to be higher than that of the control. The total monomeric anthocyanin of the U3 and U8 applications was higher than that of the control. Furthermore, the antioxidant activity of the U2, U3 and U5 in the DPPH, and the U7 and U8 in FRAP were measured higher thanthat of the control. Conclusions The application of biofilms has the potential to mitigate fruit cracking, prolong postharvest life of sweet cherries, and enhance fruit firmness.

The pH and firmness index (FI) of red globe grapes determine the taste and post-harvest quality of the fruit. In this paper, a method for the detection of pH and firmness of red globe grapes based on transmission spectroscopy technology was proposed. Spectral datas were first collected from 360 full-growth-cycle red globe grape samples, which were pre-processed and modeled by different spectral pre-processing methods as a way to determine the best spectral pre-processing method. Then competitive adaptive reweighted sampling (CARS), successive projection algorithm (SPA), uniformative variable elimination (UVE) and CARS-SPA, UVE-SPA composite data dimensionality reduction methods were used respectively for extracting feature variables from spectra. Finally, partial least squares regression (PLSR) detection models for pH and firmness of red globe grapes were established, respectively. The optimal prediction models for pH and firmness of red globe grape samples were moving-average method (MA)-CARS-SPA-PLSR and MA-UVE-SPA-PLSR. The correlation coefficient of prediction (RP) of the prediction sets of the two models were 0.9882 and 0.9588, and the residual predictive deviation (RPD) were 6.5857 and 3.5167, respectively. The results showed that transmission spectroscopy could be applied to the detection of pH and firmness of red globe grapes, which provided a new idea and a new method for the detection of pH and firmness of red globe grapes in the whole growth cycle.

IntroductionCherry, with the scientific name Prunus avium L., is one of the most important stone fruit trees in temperate regions, belonging to the Rosaceae family and the Prunoideae subfamily. Edible coatings are thin layers of materials that are used on the surface of the product and are an alternative to protective wax coatings. Chitosan is a coating that has a polysaccharide structure and is composed of glucosamine and N-acetylglucosamine units and is obtained from the shell of crustaceans such as crabs and shrimps. Clear, odorless, non-sticky gel with high and firm absorption power is extracted from the inner parts of aloe vera plant leaves. Between this gel and the outer skin of the leaf, there are special cells that contain a yellow liquid and when this liquid dries, aloe vera juice is produced. This gel is completely healthy and compatible with the environment, and its pH is about 4.5, which can replace various fruit coatings in the post-harvest stage. The purpose of this research was to investigate the combined effect of aloe vera gel and chitosan in maintaining the quality characteristics post harvesting and increasing the shelf life of Lombard cherry fruit. Materials and MethodsLambert black cherry fruit was obtained from the garden complex of Moghan Agriculture and Animal Husbandry Company located in Pars Abad city of Ardabil province. they were immediately transferred to the Postharvest Physiology Laboratory, Department of Horticultural Sciences, Faculty of Agriculture and Natural Resources, Mohaghegh Ardabili University, for the desired treatments.This study aimed to extend the shelf life of cherry fruit with two edible chitosan coatings (0, 0.5, 1, 1.5% w/w) and aloe vera gel (0, 15%, 30%, 45% w/w) was performed as factorial in a completely randomized design in three replications. The measured parameters were soluble solids, vitamin C, total acidity, anthocyanin, starch, weight loss and firmness of fruit tissue that were evaluated at harvest time and 45 days after harvest. Results and DiscussionThe results of analysis variance showed that the effect of time had significant on cherry fruit flavor (P

Background: Fruit cracking impacts the quality of sweet cherry, significantly affecting its marketability due to increased susceptibility to injury, aesthetic flaws, and susceptibility to pathogens. The effect of 1% biofilm (Parka™) application regimes on fruit cracking and other quality parameters in the '0900 Ziraat' cherry cultivar was investigated in this study. Fruit sprayed with water were served as control (U1). Fruit treated only once with biofilm three, two and one week before the commercial harvest were considered as U2, U3 and U4, respectively. Fruit treated with biofilm three, two, and one week before harvest were considered as U5; three and two week before harvest as U6; two and one week before harvest as U7; and fruit treated three and one week before harvest as U8. Results: In both measurement periods, the lower cracking index was obtained in biofilm-treated sweet cherry fruit. However, the firmness of biofilm-treated fruit was higher than that of the control fruit. The lowest respiration rate was observed in U7, while the highest weight was recorded in U4 and U5 than the control. The biofilm application decreased fruit coloration. The biofilm application also increased the soluble solids content of the fruit. The U2, U3 and U4 applications at harvest showed higher titratable acidity than the control. In both measurement periods, the vitamin C content of the U2, U5, U6, U7 and U8 applications was found to be higher than that of the control. The total monomeric anthocyanin of the U3 and U8 applications was higher than that of the control. Furthermore, the antioxidant activity of the U2, U3 and U5 in the DPPH, and the U7 and U8 in FRAP were measured higher thanthat of the control. Conclusions: The application of biofilms has the potential to mitigate fruit cracking, prolong postharvest life of sweet cherries, and enhance fruit firmness. [ABSTRACT FROM AUTHOR]

Guava fruit is an abundant source of phytonutrients namely, vitamin C, carotenoids, minerals and polyphenols. However, due to its climacteric nature, fruit are highly perishable and susceptible to low temperature storage resulting in poor shelf life. The current investigation emphasizes the antioxidant potential of melatonin (MT) on ripening and quality of guava (cv. Barafkhana) fruit during cold storage. Physiologically mature fruit were immersed in MT solutions (200, 400, 600, and 800 μmol L−1) for 30 min at 20 ± 2 °C. Subsequently, the fruit were stored in cold storage at 10 ± 1 °C and 85–90 % relative humidity for 20 days. In results, MT at 600 μmol L−1 significantly suppressed the ethylene evolution and respiration rate, and reduced weight loss, while retaining firmness. Also, higher levels of total soluble solids, total sugars and total chlorophyll were retained in MT treated fruit over control. Bioactive compounds such as ascorbic acid, total phenols, flavonoids and antioxidant activity were high in fruit subjected to MT treatment. Furthermore, exogenous MT application prevented the accumulation of malondialdehyde and hydrogen peroxide, indicating a potential inhibition of reactive oxygen species (ROS) burst and oxidative damage. Moreover, MT application boosted the scavenging capacity of ROS by enhancing the activities of superoxide dismutase, catalase, guaiacol peroxidase, and ascorbate peroxidase. The multivariate statistical approaches such as principal component analysis and Pearson's correlation coefficient also confirmed that MT (600 μmol L−1) is a potential novel phytohormone to preserve the sensory quality of guava fruit during cold storage via stimulating antioxidant system. [Display omitted] • Postharvest melatonin (MT) application (600 μmol L−1) preserved quality of stored guava fruit up to 20 days. • Delayed fruit senescence, suppressed ethylene production and respiration rates and retained firmness, TSS and chlorophyll. • MT alleviated oxidative stress and preserved cell membrane integrity. • MT stimulated the antioxidant system via enhancing the antioxidant enzyme activities (SOD, CAT, APX and GPX). [ABSTRACT FROM AUTHOR]

Tomato (Solanum lycopersicum) is a widely consumed vegetable crop with significant economic and nutritional importance. This review paper discusses the recent advancements in gene-based approaches to enhance the quality of tomatoes, particularly focusing on firmness, shelf life, and adaptations to pre- and post-harvest stresses. Utilizing genetic engineering techniques, such as Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated proteins 9 (CRISPR/Cas9) and Transcription Activator-like Effector Nucleases (TALENs), researchers have made remarkable progress in developing tomatoes with improved traits that address key challenges faced during cultivation, storage, and transportation. We further highlighted the potential of genetic modifications in enhancing tomato firmness, thereby reducing post-harvest losses and improving consumer satisfaction. Furthermore, strategies to extend tomato shelf life through genetic interventions are discussed, emphasizing the importance of maintaining quality and freshness for sustainable food supply chains. Furthermore, the review delves into the ways in which gene-based adaptations can bolster tomatoes against environmental stresses, pests, and diseases, thereby enhancing crop resilience and ensuring stable yields. Emphasizing these crucial facets, this review highlights the essential contribution of genetic advancements in transforming tomato production, elevating quality standards, and promoting the sustainability of tomato cultivation practices. [ABSTRACT FROM AUTHOR]

The pH and firmness index (FI) of red globe grapes determine the taste and post-harvest quality of the fruit. In this paper, a method for the detection of pH and firmness of red globe grapes based on transmission spectroscopy technology was proposed. Spectral datas were first collected from 360 full-growth-cycle red globe grape samples, which were pre-processed and modeled by different spectral pre-processing methods as a way to determine the best spectral pre-processing method. Then competitive adaptive reweighted sampling (CARS), successive projection algorithm (SPA), uniformative variable elimination (UVE) and CARS-SPA, UVE-SPA composite data dimensionality reduction methods were used respectively for extracting feature variables from spectra. Finally, partial least squares regression (PLSR) detection models for pH and firmness of red globe grapes were established, respectively. The optimal prediction models for pH and firmness of red globe grape samples were moving-average method (MA)-CARS-SPA-PLSR and MA-UVE-SPA- PLSR. The correlation coefficient of prediction (RP) of the prediction sets of the two models were 0.9882 and 0.9588, and the residual predictive deviation (RPD) were 6.5857 and 3.5167, respectively. The results showed that transmission spectroscopy could be applied to the detection of pH and firmness of red globe grapes, which provided a new idea and a new method for the detection of pH and firmness of red globe grapes in the whole growth cycle. [ABSTRACT FROM AUTHOR]

From economic and nutritional points of view, tomato is, historically, considered one of the most important crops. Without significant yield reduction, most commercial cultivars of tomato crops are sensitive to moderate levels of salinity. However, high levels of salt stress can negatively affect the yield and quality of tomato fruits. Therefore, this study was conducted to evaluate the yield and fruit quality of three tomato cultivars (Forester, Ghandowra-F1, and Feisty-Red) cultivated hydroponically, under three different levels of nutrient solution salinity. Evaluation of tomato fruits was performed based on quantity (number and weight of fruits, and total fruit yield), physical quality (color and firmness), and chemical quality (refractometric index "Brix", pH, EC, K+, Na+, and NO3-). Experiments were conducted using three salinity levels of the nutrient solution with electrical conductivity values (dSm-1) of 2.5 (control), 6.0, and 9.5. Results showed that the studied tomato cultivars were significantly influenced by high salinity (9.5 dSm-1) in comparison to the low (2.5 dSm-1) and medium (6.0 dSm-1) levels of salinity. On average, the highest fruit weight per plant of 1944.84 g and total fruit yield of 4.42 kgm-2 were observed at the low salinity level; however, no significant differences were obtained in the two yield factors (single fruit weight and total fruit yield) for the low and medium salinity levels. On the other hand, a significant reduction in tomato yield (31%) was associated with the high salinity level compared to the yield at low and medium salinity levels. Results of physical quality parameters showed highly significant differences among all salinity levels. On average, the maximum value of color change (1.72) was associated with the medium salinity level, and the maximum value of firmness (9.61 Ncm-1) was recorded at the high salinity level. Salinity levels and tomato cultivars introduced significant differences in chemical quality parameters; however, no significant differences in these parameters were attributed to the low and medium salinity levels. The maximum value of pH was recorded for the combination of medium salinity and Forester cultivar. Moreover, the maximum values of Brix, EC, K+, Na+, and NO3- were recorded for the Ghandowra-F1 cultivar at the high salinity level. Unlike the Feisty-Red, the performance of the Forester and Ghandowra-F1 cultivars was found to be acceptable at the tested medium salinity level (6.0 dSm-1). [ABSTRACT FROM AUTHOR]

Nanofertilizers represent an efficient method for fertilizing crops, with a positive impact on plant development. This work aimed to determine the impact of Nubiotek Ultra Ca and Nubiotek Hyper Fe +Mg nanofertilizers on the yield and quality of cucumber fruits in 2020. Two doses of Nubiotek Ultra Ca (0 and 20 L ha-1) and four doses of Nubiotek Hyper Fe+Mg (0, 2, 4, and 8 ml L-1) were evaluated. An experimental design of randomized complete blocks was used with a factorial arrangement (2x4), with four repetitions. The results showed that the nanofertilizers evaluated did not improve internode distance, dry weight of each organ, root length, total soluble solids, vitamin C, titratable acidity, and fruit color parameters compared to the values obtained with the control. On the other hand, with the treatments of 0 L ha-1 Nubiotek Ultra Ca + 8 ml L-1 Nubiotek Hyper Fe+Mg and 20 L ha-1 Nubiotek Ultra Ca + 2 ml L-1 Nubiotek Hyper Fe+Mg, the fruit yield increased by 111 and 123%, respectively, compared to the control. Likewise, a greater firmness of fruits was observed when applying Nubiotek Ultra Ca and Nubiotek Hyper Fe+Mg in doses of 20 L ha-1 + 8 ml L-1; on the other hand, the highest content of chlorophyll a, b and total was achieved with doses of 20 L ha-1 + 4 ml L-1 and 20 L ha-1 + 8 ml L-1. [ABSTRACT FROM AUTHOR]