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In this study, indigo-dyed denim fabric was decolorized by washing and printing with separate and simultaneous applications of laccase enzyme, sodium hydrosulfite, and cellulase enzyme. In this regard, the surface reflectance and color coordinates of the discolored fabrics were analyzed, and SEM photographs of the treated fabrics were prepared to analyze their surfaces. Finally, the effects of the discoloration process and materials on various parameters of the treated samples were investigated, including moisture content, creaserecovery angle, air permeability, and abrasion resistance. The color experiments showed that the discoloration mechanism with the combined use of laccase enzyme, sodium hydrosulfite, and cellulose enzyme had a significant effect on the improvement of the lightness (L*) of the samples, as the lightness of the treated samples was improved by 101.18 percent and 55.79 percent in both printing and washing, respectively. Furthermore, examination of specimen color coordinates revealed that the hue of the treated samples was changed to blue and green, and the purity of color (C*) was improved. The increased moisture content and air permeability of the treated specimens suggested that the comfort of the jeans clothing provided by these treatment methods had improved. As a result, it should be noted that the mediating action of sodium hydrosulfite was significantly influential for discoloration of denim with the laccase enzyme.

Implant-related infection is one of the main challenges in periodontal diseases. According to the zwitterionic properties of keratin, we aim to develop guided bone regeneration (GBR) membrane with antibacterial and bioactivity properties using a keratin coating. In this study, electrospun silk fibroin (SF)-Laponite (LAP) fibrous membranes were developed as GBR membranes, and keratin extracted from sheep wool was electrosprayed on them. Here, the role of electrospraying time (2, 3, and 4h) on the properties of the GBR membranes was investigated. After physicochemical characterization of the keratin-modified membranes, in vitro bioactivity and degradation rate of the membranes were studied in simulated body fluid and phosphate buffer saline, respectively. Moreover, proliferation and differentiation of mesenchymal stem cells were evaluated in contact with the keratin-modified SF-LAP membrane. Finally, the antibacterial activity of membranes against gram-positive bacteria (Staphylococcus aureus) was investigated. Results demonstrated the successful formation of homogeneous wool keratin coating on SF-LAP fibrous membranes using a simple electrospray process. While wool keratin coating significantly enhanced the elongation and hydrophilicity of the SF-LAP membrane, the mechanical strength was not changed. In addition, keratin coating significantly improved the bioactivity and degradation rate of SF-LAP membranes, owing to the carboxyl groups of amino acids in keratin coating. In addition, the synergic role of LAP nanoparticles and keratin coating drastically improved osteoblast proliferation and differentiation. Finally, the zwitterionic property of wool keratin coating originating from their equal positive (NH