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Your search keyword '"Dudczig, Stefanie"' showing total 10 results
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10 results on '"Dudczig, Stefanie"'

1. The RNA helicase Ddx21 controls Vegfc-driven developmental lymphangiogenesis by balancing endothelial cell ribosome biogenesis and p53 function

Author

Koltowska, Katarzyna, Okuda, Kazuhide S., Gloger, Marleen, Rondon-Galeano, Maria, Mason, Elizabeth, Xuan, Jiachen, Dudczig, Stefanie, Chen, Huijun, Arnold, Hannah, Skoczylas, Renae, Bower, Neil I., Paterson, Scott, Lagendijk, Anne Karine, Baillie, Gregory J., Leshchiner, Ignaty, Simons, Cas, Smith, Kelly A., Goessling, Wolfram, Heath, Joan K., Pearson, Richard B., Sanij, Elaine, Schulte-Merker, Stefan, and Hogan, Benjamin M.

Published
2021
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3. Unraveling the evolutionary origin of the complex Nuclear Receptor Element (cNRE), a cis-regulatory module required for preferential expression in the atrial chamber.

Author

Nunes Santos, Luana, Sousa Costa, Ângela Maria, Nikolov, Martin, Carvalho, João E., Coelho Sampaio, Allysson, Stockdale, Frank E., Wang, Gang Feng, Andrade Castillo, Hozana, Bortoletto Grizante, Mariana, Dudczig, Stefanie, Vasconcelos, Michelle, Rosenthal, Nadia, Jusuf, Patricia Regina, Nim, Hieu T., de Oliveira, Paulo, Guimarães de Freitas Matos, Tatiana, Nikovits Jr., William, Tambones, Izabella Luisa, Figueira, Ana Carolina Migliorini, and Schubert, Michael

Subjects
GENE expression, BASE pairs, ATRIUMS (Architecture), COMPARATIVE genomics, HEART diseases, MYOSIN
Abstract

Cardiac function requires appropriate proteins in each chamber. Atria requires slow myosin to act as reservoirs, while ventricles demand fast myosin for swift pumping. Myosins are thus under chamber-biased cis-regulation, with myosin gene expression imbalances leading to congenital heart dysfunction. To identify regulatory inputs leading to cardiac chamber-biased expression, we computationally and molecularly dissected the quail Slow Myosin Heavy Chain III (SMyHC III) promoter that drives preferential expression to the atria. We show that SMyHC III gene states are orchestrated by a complex Nuclear Receptor Element (cNRE) of 32 base pairs. Using transgenesis in zebrafish and mice, we demonstrate that preferential atrial expression is achieved by a combinatorial regulatory input composed of atrial activation motifs and ventricular repression motifs. Using comparative genomics, we show that the cNRE might have emerged from an endogenous viral element through infection of an ancestral host germline, revealing an evolutionary pathway to cardiac chamber-specific expression. A complex Nuclear Receptor Element (cNRE) of 32 base pairs that emerged from a viral infection drives the quail Slow Myosin Heavy Chain III (SMyHC III) promoter preferential expression to the atria. [ABSTRACT FROM AUTHOR]

Published
2024
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4. Unraveling the evolutionary origin of the complex Nuclear Receptor Element (cNRE), a cis-regulatory module required for preferential expression in the atrial chamber

Author

Neto, Jose Xavier, primary, Santos, Luana Nunes, additional, Costa, Angela Maria Sousa, additional, Nikolov, Martin, additional, Carvalho, João, additional, Sampaio, Allysson Coelho, additional, Stockdale, Frank, additional, Castillo, Hozana Andrade, additional, Grizante, Mariana Bortoletto, additional, Dudczig, Stefanie, additional, Vasconcelos, Michelle, additional, Rosenthal, Nadia, additional, Jusuf, Patricia, additional, Nim, Hieu, additional, Lopes-de-Oliveira, Paulo, additional, Matos, Tatiana Guimaraes de Freitas, additional, Nikovits, William, additional, Schubert, Michael, additional, and Ramialison, Mirana, additional

Published
2023
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5. Single‐cell analysis of lymphatic endothelial cell fate specification and differentiation during zebrafish development

Author

Grimm, Lin, primary, Mason, Elizabeth, additional, Yu, Hujun, additional, Dudczig, Stefanie, additional, Panara, Virginia, additional, Chen, Tyrone, additional, Bower, Neil I, additional, Paterson, Scott, additional, Rondon Galeano, Maria, additional, Kobayashi, Sakurako, additional, Senabouth, Anne, additional, Lagendijk, Anne K, additional, Powell, Joseph, additional, Smith, Kelly A, additional, Okuda, Kazuhide S, additional, Koltowska, Katarzyna, additional, and Hogan, Benjamin M, additional

Published
2023
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7. Single cell analysis of lymphatic endothelial cell fate specification and differentiation during zebrafish development

Author

Grimm, Lin, Mason, Elizabeth, Yu, Oliver, Dudczig, Stefanie, Panara, Virginia, Chen, Tyrone, Bower, Neil I., Paterson, Scott, Okuda, Kazuhide, Rondon Galeano, Maria, Kobayashi, Sakurako, Senabouth, Anne, Lagendijk, Anne K., Powell, Joseph, Smith, Kelly A., Koltowska, Katarzyna, Hogan, Benjamin M., Grimm, Lin, Mason, Elizabeth, Yu, Oliver, Dudczig, Stefanie, Panara, Virginia, Chen, Tyrone, Bower, Neil I., Paterson, Scott, Okuda, Kazuhide, Rondon Galeano, Maria, Kobayashi, Sakurako, Senabouth, Anne, Lagendijk, Anne K., Powell, Joseph, Smith, Kelly A., Koltowska, Katarzyna, and Hogan, Benjamin M.

Abstract

During development, the lymphatic vasculature forms as a second, new vascular network derived from blood vessels. The transdifferentiation of embryonic venous endothelial cells (VECs) into lymphatic endothelial cells (LECs) is the first step in this process. Specification, differentiation and maintenance of LEC fate are all driven by the transcription factor Prox1, yet downstream mechanisms remain to be elucidated. We present a single cell transcriptomic atlas of lymphangiogenesis in zebrafish revealing new markers and hallmarks of LEC differentiation over four developmental stages. We further profile single cell transcriptomic and chromatin accessibility changes in zygotic prox1a mutants that are undergoing a VEC-LEC fate reversion during differentiation. Using maternal and zygotic prox1a/prox1b mutants, we determine the earliest transcriptomic changes directed by Prox1 during LEC specification. This work altogether reveals new transcriptional targets and regulatory regions of the genome downstream of Prox1 in LEC maintenance, as well as showing that Prox1 specifies LEC fate primarily by limiting blood vascular and hematopoietic fate. This extensive single cell resource provides new mechanistic insights into the enigmatic role of Prox1 and the control of LEC differentiation in development.

Published
2023

8. Author Reply to Peer Reviews of Single cell analysis of lymphatic endothelial cell fate specification and differentiation during zebrafish development

Author

Grimm, Lin, primary, Mason, Elizabeth A, additional, Yu, Oliver, additional, Dudczig, Stefanie, additional, Panara, Virginia, additional, Chen, Tyrone, additional, Bower, Neil I, additional, Paterson, Scott, additional, Okuda, Kazuhide, additional, Rondon Galeano, Maria, additional, Kobayashi, Sakurako, additional, Senabouth, Anne, additional, Lagendijk, Anne, additional, Powell, Joseph, additional, Smith, Kelly A, additional, Koltowska, Katarzyna, additional, and Hogan, Benjamin M, additional

Published
2022
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9. Single cell analysis of lymphatic endothelial cell fate specification and differentiation during zebrafish development

Author

Grimm, Lin, primary, Mason, Elizabeth, additional, Yu, Oliver, additional, Dudczig, Stefanie, additional, Panara, Virginia, additional, Chen, Tyrone, additional, Bower, Neil I., additional, Paterson, Scott, additional, Okuda, Kazuhide, additional, Galeano, Maria Rondon, additional, Kobayashi, Sakurako, additional, Senabouth, Anne, additional, Lagendijk, Anne K., additional, Powell, Joseph, additional, Smith, Kelly A., additional, Koltowska, Katarzyna, additional, and Hogan, Benjamin M., additional

Published
2022
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10. A tripartite structure, the complex nuclear receptor element (cNRE), is a cis-regulatory module of viral origin required for atrial chamber preferential gene expression

Author

Nunes Santos, Luana, primary, Costa, Angela, additional, Nikolov, Martin, additional, Sampaio, Allysson Coelho, additional, Stockdale, Frank, additional, Castillo, Hozana Andrade, additional, Grizante, Mariana Bortoletto, additional, Dudczig, Stefanie, additional, Vasconcelos, Michelle, additional, Rosenthal, Nadia, additional, Jusuf, Patricia Regina, additional, Lopes-de-Oliveira, Paulo, additional, Matos, Tatiana, additional, Nikovits, Wiliam, additional, Schubert, Michael, additional, Ramialison, Mirana, additional, and Xavier-Neto, Jose, additional

Published
2021
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