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Ebony (Diospyroscelebica.Bakh) is an Indonesian medicinal plant used as antidiabetic herbs. A previous study found that the ethanol extract of ebony leaf has strong antioxidant and αglucosidase inhibitory activity. The aim of this study was to isolate and characterize antioxidant and antidiabetic compounds from the leaves of Ebony. Powdered ebony leaves were extracted with 96% ethanol, and then fractionated with ethyl acetate. The ethyl acetate fraction was purified by column chromatography which led to the isolation of compound Fr.36-13a.The structure of compoundFr.36-13awas elucidated through a combination of ¹H-NMR, 13C-NMR, and 2D-NMR spectroscopy. The antioxidant activity of compound Fr.36-13a was determined by DPPH (1,1-diphenyl-2-picryl hydrazyl) radical scavenging assay. The antidiabetic activity of the compound was assessed in vitro by the α-glucosidase inhibitory assay. Compound Fr.36-13a was identified as kaempferol-3-O-β-glucoside through the 1D and 2D NMR data, and by comparison of the spectroscopic data with literature values. Compound Fr.36-13a (Kaempferol3-O-β-glucoside) showed strong antioxidant and antidiabetic activities by scavenging DPPH radical and inhibiting α-glucosidase enzyme. Kaempferol-3-O-β-glucoside could serve as a potential therapeutic agents for the treatment of diabetes mellitus. [ABSTRACT FROM AUTHOR]

Macassar ebony (Diospyros celebica Bakh.) is an endemic tree species native to Sulawesi. To date, there are limited comprehensive publications regarding its genome assembly. In this study, we employed paired-end libraries of HiSeq 4000 Illumina, generating 141.2 million paired-end reads (42.4 Gigabases). Our analysis revealed the presence of 950,081 Scaffolds, with an N50 value of 6,023. Notably, BUSCO analysis identified 183 (12.7%) complete and single-copy BUSCOs (S), as well as 9 (0.6%) complete and duplicated BUSCOs (D). Furthermore, we identified 12,890 microsatellites within the Macassar Ebony genome. These microsatellites encompass 14 dinucleotide SSR motifs, 12,090 trinucleotide SSRs, 780 tetranucleotide SSR motifs, and 6 pentanucleotide SSRs. This dataset represents a valuable resource for assessing Macassar Ebony genetic makeup in its natural habitats and for subsequent analyses of the Macassar Ebony genome. [ABSTRACT FROM AUTHOR]