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Cancer stem cells (CSCs) cause drug resistance in cancer due to its extensive drug efflux, DNA repair and self-renewal capability. ATP binding cassette subfamily G member 2 (ABCG2) efflux pump afford protection to CSCs in tumors, shielding them from the adverse effects of chemotherapy. Although the role of ABCG2 in cancer progression, invasiveness, recurrence are known but its role in metastasis and angiogenesis are not clear. Here, using in vitro (CSCs enriched side population [SP] cells), ex vivo (patient derived primary cells), in ovo (fertilized egg embryo) and in vivo (patient derived primary tissue mediated xenograft (PDX)) system, we have systematically studied the role of ABCG2 in angiogenesis and the regulation of the process by Curcumin (Cur) and Quinacrine (QC). Cur + QC inhibited the proliferation, invasion, migration and expression of representative markers of metastasis and angiogenesis. Following hypoxia, ABCG2 enriched cells released angiogenic factor vascular endothelial growth factor A (VEGF A) and induced the angiogenesis via PI3K-Akt-eNOS cascade. Cur + QC inhibited the ABCG2 expression and thus reduced the angiogenesis. Interestingly, overexpression of ABCG2 in SP cells and incubation of purified ABCG2 protein in media induced the angiogenesis but knockdown of ABCG2 decreased the vascularization. In agreement with in vitro results, ex vivo data showed similar phenomena. An induction of vascularization was noticed in PDX mice but reduction of vascularization was also observed after treatment of Cur + QC. Thus, data suggested that in hypoxia, ABCG2 enhances the production of angiogenesis factor VEGF A which in turn induced angiogenesis and Cur + QC inhibited the process by inhibiting ABCG2 in breast cancer. [ABSTRACT FROM AUTHOR]

In this work, we used the semi-Lagrangian method and the Vlasov code to study the instabilities in a quantum plasma with 1-D temperature anisotropy. Taking the Fermi–Dirac type of distribution function (DF) and using the semi-Lagrangian Vlasov code, we studied the gradual evolution of density and kinetic energy in a degenerate plasma with quantum effects. In this collisionless plasma, adiabatic compression will be along the direction of wave propagation and will lead to temperature anisotropy of the electron distribution that varies along the direction of wave propagation. The findings will find application for dense plasma at finite temperature as in laser-produced plasmas, fusion plasma, as well as solar plasmas. [ABSTRACT FROM AUTHOR]

The effects of developing employees' career abilities (knowing-why, knowing-how, and knowing-whom) on the employing organisation are examined in this study, as well as the function of career happiness in this relationship. The goal of this paper is to contribute to the growing body of knowledge about the effects of employees' boundaryless careers A two-wave quantitative investigation of graduates of a large public university in Hyderabad is used in this study. The findings imply that individuals contribute to company culture, competences, and connections by investing in the development of their career competencies. These contributions, however, are contingent on employees' perceptions of career satisfaction. Employees that are satisfied in their jobs are more likely to give more to their companies. Research limitations/implications - To replicate the current findings, more longitudinal research is needed, supported by a third wave of data collecting. The study emphasises the relevance of career development for employees in the workplace. This is an empirical assessment of the relationship between the development of employees' career competences and their contributions to their employers, as well as one of the first studies to look at career satisfaction as a moderating variable. [ABSTRACT FROM AUTHOR]

Background Anticipatory heart rate (HR) refers to an increase in HR that occurs in anticipation of a future event or activity. The anticipatory heart rate (HR) response before exercise is an important physiological indicator of exercise readiness. This study aimed to compare the anticipatory HR changes between sedentary and physically active young adult males during moderate- and vigorous-intensity exercise. Understanding these anticipatory heart rate patterns can provide insights into the physiological adaptations and cardiovascular health of individuals with varying physical activity levels. Materials and methods A total of 60 young adult males, comprising sedentary (n = 30) and physically active individuals (n = 30), participated in this study. A brisk walking for a distance of 50 m was considered moderate intensity and one minute of spot jogging at maximum effort with verbal encouragement was considered vigorous intensity exercise. The HR was recorded at baseline, just before the exercise, and on each minute up to 5 minutes after the exercise. Results The study involved 30 physically active young adult males (mean age 20.23 ± 1.43 years) and 30 sedentary adult males (mean age 20.07 ± 1.17 years). In physically active young adults, the resting HR was 76.4±10.89 bpm and just before starting moderate-intensity exercise, it was 78.83±12.98 bpm, paired t-test P = 0.22. The HR just before vigorous-intensity exercise was 80.83±11.18 bpm (paired t-test P = 0.03). In sedentary young adults, the resting HR was 82.23±12.69 bpm and just before starting moderate-intensity exercise, it was 90.13±18.69 bpm, paired t-test P = 0.0008. The HR just before vigorous-intensity exercise was 91.7±15.04 bpm (paired t-test P <0.0001). Conclusion Physically active young adults did not exhibit a significant increase in anticipatory HR before moderate-intensity exercise. However, sedentary individuals exhibit a significant anticipatory HR response. Before vigorous-intensity exercise, both exhibited significant increments in HR. The result highlights the importance of considering the anticipatory HR response as a potential marker of cardiovascular health and exercise readiness., Competing Interests: The authors have declared that no competing interests exist., (Copyright © 2023, Das et al.)