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In terrestrial biosphere, soil represents the largest organic carbon pool, and a small change of soil organic carbon (SOC) can significantly affect the global carbon cycle and climate. Land use change (LUC) and soil management practices coupled with climate variables can significantly influence the soil organic carbon stocks (SOC–S) and its dynamics; however, our understanding about the responses of SOC in different LUC's (e.g., cropland, grassland and forest land) to mitigate climate change is quite limited at country level like Italy. Thus, the aims of this study were which factors are affecting SOC dynamics in three LUC's over time across Italy; and their relevance in terms of SOC–S in the superficial layer of soil that significantly contributes to the climate change mitigation, using LUCAS soil database. To calculate the SOC–S, it is necessary to have soil bulk density (BD) which is not present in the LUCAS database. Thus, we estimate the soil BD using the pedotransfer function (PTFs); and results shows that the soil BD obtained from fitting of the PTFs were reasonable to estimate the SOC–S for different land use types (R2 ≥ 0.75). Overall, results showed that LUC's and soil management practices can significantly (p < 0.001) influences SOC dynamics and SOC storage from the soil and varied among LUC's but not for over time except grassland. Spatially, the mean SOC–S storage of the different LUC's was in the following order: forest land > grassland > cropland for both years 2009 and 2015. On the other hand, the SOC–S storage increased by 8.33% for cropland, 13.56% for forest land, and 29.79% for grassland during the year of 2009–2015, while SOC–S storage increased significantly (p < 0.001) in grassland over time but not for cropland and forest land which also follow the increasing trend but insignificantly. Our results also reveal that the SOC dynamics negatively correlated with MAT, and positively correlated with MAP for all land uses except forest land. Thus, this research indicates that LUC's and soil management practices coupled with climate variables can significantly influence SOC storage and its dynamics in the superficial layer of soil which have the potential capacity to mitigate climate change. [Display omitted] • Using PTFs for obtaining soil BD was reasonable to estimate the SOC–S for different LUTs. • SOC negatively correlated with MAT and positively correlated with MAP for all LUT except forest land. • Among LUTs, forest land could store highest SOC in both years compared to grassland and cropland. • Over time, SOC–S increased significantly (p < 0.001) in grassland but not for forest land and cropland. [ABSTRACT FROM AUTHOR]