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Your search keyword '"Capo, Alessandro"' showing total 9 results
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9 results on '"Capo, Alessandro"'

1. Ultra-Fast Detection of Pathogens and Protein Biomarkers Using a Low-Cost Silicon Plasmonic Biosensing Platform

Author

Manolis, Athanasios, primary, Eleftheriou, Christia, additional, Elrabiaey, Mahmoud A., additional, Tsekenis, George, additional, D'Auria, Sabato, additional, Varriale, Antonio, additional, Capo, Alessandro, additional, Staiano, Maria, additional, Chmielak, Bartos, additional, Schall-Giesecke, Anna Lena, additional, Suckow, Stephan, additional, and Tsiokos, Dimitris, additional

Published
2024
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2. Ultra-Fast Detection of Pathogens and Protein Biomarkers Using a Low-Cost Silicon Plasmonic Biosensing Platform for Efficient Management of Sepsis at the Point of Care

Author

Manolis, Athanasios, primary, Eleftheriou, Christia, additional, Elrabiaey, Mahmoud A., additional, Tsekenis, George, additional, D'Auria, Sabato, additional, Varriale, Antonio, additional, Capo, Alessandro, additional, Staiano, Maria, additional, Chmielak, Bartos, additional, Schall-Giesecke, Anna Lena, additional, Suckow, Stephan, additional, and Tsiokos, Dimitris, additional

Published
2024
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3. A Förster Resonance Energy Transfer (FRET)-Based Immune Assay for the Detection of Microcystin-LR in Drinking Water.

Author

Capo, Alessandro, Pennacchio, Angela, Montagnese, Concetta, Hadjiantonis, Antonis, Demosthenous, Panayiota, Giusti, Alessandro, Staiano, Maria, D'Auria, Sabato, and Varriale, Antonio

Subjects
*FLUORESCENCE resonance energy transfer, *MICROCYSTINS, *CYANOBACTERIAL blooms, *AMINO acid residues, *ANIMAL health, *ENERGY transfer, *DRINKING water
Abstract

Cyanobacteria bloom is the term used to describe an abnormal and rapid growth of cyanobacteria in aquatic ecosystems such as lakes, rivers, and oceans as a consequence of anthropic factors, ecosystem degradation, or climate change. Cyanobacteria belonging to the genera Microcystis, Anabaena, Planktothrix, and Nostoc produce and release toxins called microcystins (MCs) into the water. MCs can have severe effects on human and animal health following their ingestion and inhalation. The MC structure is composed of a constant region (composed of five amino acid residues) and a variable region (composed of two amino acid residues). When the MC variable region is composed of arginine and leucine, it is named MC-LR. The most-common methods used to detect the presence of MC-LR in water are chromatographic-based methods (HPLC, LC/MS, GC/MS) and immunological-based methods (ELISA). In this work, we developed a new competitive Förster resonance energy transfer (FRET) assay to detect the presence of traces of MC-LR in water. Monoclonal antibody anti-MC-LR and MC-LR conjugated with bovine serum albumin (BSA) were labeled with the near-infrared fluorophores CF568 and CF647, respectively. Steady-state fluorescence measurements were performed to investigate the energy transfer process between anti-MC-LR 568 and MC-LR BSA 647 upon their interaction. Since the presence of unlabeled MC-LR competes with the labeled one, a lower efficiency of FRET process can be observed in the presence of an increasing amount of unlabeled MC-LR. The limit of detection (LoD) of the FRET assay is found to be 0.245 nM (0.245 µg/L). This value is lower than the provisional limit established by the World Health Organization (WHO) for quantifying the presence of MC-LR in drinking water. [ABSTRACT FROM AUTHOR]

Published
2024
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7. SPR-Based Detection of ASF Virus in Cells

Author

Capo, Alessandro, primary, Calabrese, Alessia, additional, Frant, Maciej, additional, Walczak, Marek, additional, Szczotka-Bochniarz, Anna, additional, Manessis, Georgios, additional, Bossis, Ioannis, additional, Staiano, Maria, additional, D’Auria, Sabato, additional, and Varriale, Antonio, additional

Published
2022
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9. Photonic Label-Free Biosensors for Fast and Multiplex Detection of Swine Viral Diseases

Author

Gómez-Gómez, Maribel, primary, Sánchez, Carles, additional, Peransi, Sergio, additional, Zurita, David, additional, Bellieres, Laurent, additional, Recuero, Sara, additional, Rodrigo, Manuel, additional, Simón, Santiago, additional, Camarca, Alessandra, additional, Capo, Alessandro, additional, Staiano, Maria, additional, Varriale, Antonio, additional, D’Auria, Sabato, additional, Manessis, Georgios, additional, Gelasakis, Athnasios I., additional, Bossis, Ioannis, additional, Balka, Gyula, additional, Dénes, Lilla, additional, Frant, Maciej, additional, Nannucci, Lapo, additional, Bonasso, Matteo, additional, Giusti, Alessandro, additional, and Griol, Amadeu, additional

Published
2022
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