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Nutrition screening tools may facilitate early nutrition interventions specifically with Hispanic populations that are already at higher risk. There is a paucity of culturally competent and validated nutrition screening tools for 3–5-year-old Hispanic children. The purpose of this study was to develop a Spanish nutrition screening tool for 3–5-year-old children to be used by Spanish-speaking parents in community settings to appropriately address malnutrition risk factors with cultural sensitivity. All phases of the study were conducted in Spanish with native Spanish speakers. Face and content validity were established using focus groups, expert reviews, and pilot testing of the tool with Spanish-speaking parents. Parents of children 3–5 years old whose primary language was Spanish (n = 39) completed the nutrition screening, and a nutritionist completed an in-depth nutrition assessment of these children. Criterion validity was measured by comparing the results of the nutrition screening tool with the in-depth nutrition assessment. The nutrition screening tool had a sensitivity of 91.67% and a specificity of 81.48%. The negative predictive value was 69%, and the positive predictive value was 96%. The nutrition screening tool may be used to identify malnutrition in Hispanic children and needs further validation in larger samples. [ABSTRACT FROM AUTHOR]