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Your search keyword '"Barbosa, Ana B."' showing total 10 results
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10 results on '"Barbosa, Ana B."'

4. Runx1+ vascular smooth muscle cells are essential for hematopoietic stem and progenitor cell development in vivo

Author

Gonzalez Galofre, Zaniah N, Kilpatrick, Alastair M, Marques, Madalena, Sá da Bandeira, Diana, Ventura, Telma, Gomez Salazar, Mario, Bouilleau, Léa, Marc, Yvan, Barbosa, Ana B, Rossi, Fiona, Beltran, Mariana, van de Werken, Harmen J G, van IJcken, Wilfred F J, Henderson, Neil C, Forbes, Stuart J, Crisan, Mihaela, Gonzalez Galofre, Zaniah N, Kilpatrick, Alastair M, Marques, Madalena, Sá da Bandeira, Diana, Ventura, Telma, Gomez Salazar, Mario, Bouilleau, Léa, Marc, Yvan, Barbosa, Ana B, Rossi, Fiona, Beltran, Mariana, van de Werken, Harmen J G, van IJcken, Wilfred F J, Henderson, Neil C, Forbes, Stuart J, and Crisan, Mihaela

Abstract

Hematopoietic stem cells (HSCs) produce all essential cellular components of the blood. Stromal cell lines supporting HSCs follow a vascular smooth muscle cell (vSMC) differentiation pathway, suggesting that some hematopoiesis-supporting cells originate from vSMC precursors. These pericyte-like precursors were recently identified in the aorta-gonad-mesonephros (AGM) region; however, their role in the hematopoietic development in vivo remains unknown. Here, we identify a subpopulation of NG2 +Runx1 + perivascular cells that display a sclerotome-derived vSMC transcriptomic profile. We show that deleting Runx1 in NG2 + cells impairs the hematopoietic development in vivo and causes transcriptional changes in pericytes/vSMCs, endothelial cells and hematopoietic cells in the murine AGM. Importantly, this deletion leads also to a significant reduction of HSC reconstitution potential in the bone marrow in vivo. This defect is developmental, as NG2 +Runx1 + cells were not detected in the adult bone marrow, demonstrating the existence of a specialised pericyte population in the HSC-generating niche, unique to the embryo.

Published
2024

10. Short-Term Effects of Climate Change on Planktonic Heterotrophic Prokaryotes in a Temperate Coastal Lagoon: Temperature Is Good, Ultraviolet Radiation Is Bad, and CO 2 Is Neutral.

Author

Barbosa, Ana B., Mosley, Benjamin A., Galvão, Helena M., and Domingues, Rita B.

Subjects
CLIMATE change, CARBON dioxide, LAGOONS, PROKARYOTES, DEATH rate, ULTRAVIOLET radiation
Abstract

Planktonic heterotrophic prokaryotes (HProks) are a pivotal functional group in marine ecosystems and are highly sensitive to environmental variability and climate change. This study aimed to investigate the short-term effects of increasing carbon dioxide (CO2), ultraviolet radiation (UVR), and temperature on natural assemblages of HProks in the Ria Formosa coastal lagoon during winter. Two multi-stressor microcosm experiments were used to evaluate the isolated and combined effects of these environmental changes on HProk abundance, production, growth, and mortality rates. The isolated and combined effects of increased CO2 on HProks were not significant. However, HProk production, cellular activity, instantaneous growth rate, and mortality rate were negatively influenced by elevated UVR and positively influenced by warming. Stronger effects were detected on HProk mortality in relation to specific growth rate, leading to higher HProk net growth rates and abundance under elevated UVR and lower values under warming conditions. [ABSTRACT FROM AUTHOR]

Published
2023
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