Within emerging fields of research focusing on neuro‐urbanism, neuro‐geographies, and biosociality, which experiment with using emerging mobile biosensor methods, few if any have used them to research socio‐spatial life in communities that suffer high levels of violence and other socio‐spatial injustices. Extending non‐representational accounts of the body, emotions, and affect, this paper discusses an experimental geography–neuroscience collaboration, working in a favela of Rio de Janeiro to explore the embodied urban emotions and affects of violently bordered urban communities. Emphasising non‐representational, corporeal spatial practices in a study of women living in Brazil's favelas, we use electrodermal activity biosensors to propose a novel methodological and analytical approach that focuses on forms of affective debilitation and resilience. Theoretically, we draw on biopolitical theory and border theory to propose a method that avoids oppositions between biopolitical and necropolitical accounts of borders. The aim of the research, conducted in June 2016, is to understand levels of affective debilitation or resilience among women living in the Maré Complex of favelas in Rio de Janeiro. Using a wearable biosensor, we took measures of electrodermal activity of eight women as they undertook one of their routine, everyday journeys within the favela. We also conducted an hour‐long qualitative interview with each participant. We find that for all our participants, navigating the favela's violent border spaces subjects their bodies to very high levels of affective and cognitive demand. While some women responded to this with stress reactions that created acute levels of affective debilitation, others responded very strongly, showing exceptionally high levels of affective resilience. Our research highlights the affective labour required of women to co‐construct urban borders, and emphasizes their agency and forms of everyday resistance in shaping the favela's affective atmospheres. Combined biosensing (electrodermal activity/galvanic skin response) data and interview data reveal that women living in the favela experience high levels of stress and fear. For one resident this results in affective debilitation (a wounding of the capacity to affect and to be affected). However, other women showed exceptionally high levels of affective resilience and resistance. The research points to the important role of women in co‐constructing the embodied borders of the favela. [ABSTRACT FROM AUTHOR]