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180 results on '"Allentoft, Morten"'

3. Repeated plague infections across six generations of Neolithic Farmers

Author

Seersholm, Frederik Valeur, Sjögren, Karl-Göran, Koelman, Julia, Blank, Malou, Svensson, Emma M., Staring, Jacqueline, Fraser, Magdalena, Pinotti, Thomaz, McColl, Hugh, Gaunitz, Charleen, Ruiz-Bedoya, Tatiana, Granehäll, Lena, Villegas-Ramirez, Berenice, Fischer, Anders, Price, T. Douglas, Allentoft, Morten E., Iversen, Astrid K. N., Axelsson, Tony, Ahlström, Torbjörn, Götherström, Anders, Storå, Jan, Kristiansen, Kristian, Willerslev, Eske, Jakobsson, Mattias, Malmström, Helena, and Sikora, Martin

Published
2024
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4. The selection landscape and genetic legacy of ancient Eurasians

Author

Irving-Pease, Evan K, Refoyo-Martínez, Alba, Barrie, William, Ingason, Andrés, Pearson, Alice, Fischer, Anders, Sjögren, Karl-Göran, Halgren, Alma S, Macleod, Ruairidh, Demeter, Fabrice, Henriksen, Rasmus A, Vimala, Tharsika, McColl, Hugh, Vaughn, Andrew H, Speidel, Leo, Stern, Aaron J, Scorrano, Gabriele, Ramsøe, Abigail, Schork, Andrew J, Rosengren, Anders, Zhao, Lei, Kristiansen, Kristian, Iversen, Astrid KN, Fugger, Lars, Sudmant, Peter H, Lawson, Daniel J, Durbin, Richard, Korneliussen, Thorfinn, Werge, Thomas, Allentoft, Morten E, Sikora, Martin, Nielsen, Rasmus, Racimo, Fernando, and Willerslev, Eske

Subjects
Biological Sciences, Genetics, History, Heritage and Archaeology, Human Society, Archaeology, Historical Studies, Anthropology, Good Health and Well Being, Humans, Alzheimer Disease, Affect, Alleles, Agriculture, Europe, General Science & Technology
Abstract

The Holocene (beginning around 12,000 years ago) encompassed some of the most significant changes in human evolution, with far-reaching consequences for the dietary, physical and mental health of present-day populations. Using a dataset of more than 1,600 imputed ancient genomes1, we modelled the selection landscape during the transition from hunting and gathering, to farming and pastoralism across West Eurasia. We identify key selection signals related to metabolism, including that selection at the FADS cluster began earlier than previously reported and that selection near the LCT locus predates the emergence of the lactase persistence allele by thousands of years. We also find strong selection in the HLA region, possibly due to increased exposure to pathogens during the Bronze Age. Using ancient individuals to infer local ancestry tracts in over 400,000 samples from the UK Biobank, we identify widespread differences in the distribution of Mesolithic, Neolithic and Bronze Age ancestries across Eurasia. By calculating ancestry-specific polygenic risk scores, we show that height differences between Northern and Southern Europe are associated with differential Steppe ancestry, rather than selection, and that risk alleles for mood-related phenotypes are enriched for Neolithic farmer ancestry, whereas risk alleles for diabetes and Alzheimer's disease are enriched for Western hunter-gatherer ancestry. Our results indicate that ancient selection and migration were large contributors to the distribution of phenotypic diversity in present-day Europeans.

Published
2024

5. Elevated genetic risk for multiple sclerosis emerged in steppe pastoralist populations

Author

Barrie, William, Yang, Yaoling, Irving-Pease, Evan K, Attfield, Kathrine E, Scorrano, Gabriele, Jensen, Lise Torp, Armen, Angelos P, Dimopoulos, Evangelos Antonios, Stern, Aaron, Refoyo-Martinez, Alba, Pearson, Alice, Ramsøe, Abigail, Gaunitz, Charleen, Demeter, Fabrice, Jørkov, Marie Louise S, Møller, Stig Bermann, Springborg, Bente, Klassen, Lutz, Hyldgård, Inger Marie, Wickmann, Niels, Vinner, Lasse, Korneliussen, Thorfinn Sand, Allentoft, Morten E, Sikora, Martin, Kristiansen, Kristian, Rodriguez, Santiago, Nielsen, Rasmus, Iversen, Astrid KN, Lawson, Daniel J, Fugger, Lars, and Willerslev, Eske

Subjects
Biological Sciences, Genetics, History, Heritage and Archaeology, Archaeology, Historical Studies, Neurosciences, Autoimmune Disease, Multiple Sclerosis, Brain Disorders, Neurodegenerative, Human Genome, Aetiology, 2.1 Biological and endogenous factors, Inflammatory and immune system, Neurological, Humans, Neurodegenerative Diseases, Cluster Analysis, Population Density, Child, Preschool, Europe, General Science & Technology
Abstract

Multiple sclerosis (MS) is a neuro-inflammatory and neurodegenerative disease that is most prevalent in Northern Europe. Although it is known that inherited risk for MS is located within or in close proximity to immune-related genes, it is unknown when, where and how this genetic risk originated1. Here, by using a large ancient genome dataset from the Mesolithic period to the Bronze Age2, along with new Medieval and post-Medieval genomes, we show that the genetic risk for MS rose among pastoralists from the Pontic steppe and was brought into Europe by the Yamnaya-related migration approximately 5,000 years ago. We further show that these MS-associated immunogenetic variants underwent positive selection both within the steppe population and later in Europe, probably driven by pathogenic challenges coinciding with changes in diet, lifestyle and population density. This study highlights the critical importance of the Neolithic period and Bronze Age as determinants of modern immune responses and their subsequent effect on the risk of developing MS in a changing environment.

Published
2024

6. 100 ancient genomes show repeated population turnovers in Neolithic Denmark

Author

Allentoft, Morten E, Sikora, Martin, Fischer, Anders, Sjögren, Karl-Göran, Ingason, Andrés, Macleod, Ruairidh, Rosengren, Anders, Schulz Paulsson, Bettina, Jørkov, Marie Louise Schjellerup, Novosolov, Maria, Stenderup, Jesper, Price, T Douglas, Fischer Mortensen, Morten, Nielsen, Anne Birgitte, Ulfeldt Hede, Mikkel, Sørensen, Lasse, Nielsen, Poul Otto, Rasmussen, Peter, Jensen, Theis Zetner Trolle, Refoyo-Martínez, Alba, Irving-Pease, Evan K, Barrie, William, Pearson, Alice, Sousa da Mota, Bárbara, Demeter, Fabrice, Henriksen, Rasmus A, Vimala, Tharsika, McColl, Hugh, Vaughn, Andrew, Vinner, Lasse, Renaud, Gabriel, Stern, Aaron, Johannsen, Niels Nørkjær, Ramsøe, Abigail Daisy, Schork, Andrew Joseph, Ruter, Anthony, Gotfredsen, Anne Birgitte, Henning Nielsen, Bjarne, Brinch Petersen, Erik, Kannegaard, Esben, Hansen, Jesper, Buck Pedersen, Kristoffer, Pedersen, Lisbeth, Klassen, Lutz, Meldgaard, Morten, Johansen, Morten, Uldum, Otto Christian, Lotz, Per, Lysdahl, Per, Bangsgaard, Pernille, Petersen, Peter Vang, Maring, Rikke, Iversen, Rune, Wåhlin, Sidsel, Anker Sørensen, Søren, Andersen, Søren H, Jørgensen, Thomas, Lynnerup, Niels, Lawson, Daniel J, Rasmussen, Simon, Korneliussen, Thorfinn Sand, Kjær, Kurt H, Durbin, Richard, Nielsen, Rasmus, Delaneau, Olivier, Werge, Thomas, Kristiansen, Kristian, and Willerslev, Eske

Subjects
Biological Sciences, Genetics, History, Heritage and Archaeology, Human Society, Archaeology, Historical Studies, Anthropology, Humans, Genomics, Genotype, Denmark, Emigrants and Immigrants, Scandinavians and Nordic People, General Science & Technology
Abstract

Major migration events in Holocene Eurasia have been characterized genetically at broad regional scales1-4. However, insights into the population dynamics in the contact zones are hampered by a lack of ancient genomic data sampled at high spatiotemporal resolution5-7. Here, to address this, we analysed shotgun-sequenced genomes from 100 skeletons spanning 7,300 years of the Mesolithic period, Neolithic period and Early Bronze Age in Denmark and integrated these with proxies for diet (13C and 15N content), mobility (87Sr/86Sr ratio) and vegetation cover (pollen). We observe that Danish Mesolithic individuals of the Maglemose, Kongemose and Ertebølle cultures form a distinct genetic cluster related to other Western European hunter-gatherers. Despite shifts in material culture they displayed genetic homogeneity from around 10,500 to 5,900 calibrated years before present, when Neolithic farmers with Anatolian-derived ancestry arrived. Although the Neolithic transition was delayed by more than a millennium relative to Central Europe, it was very abrupt and resulted in a population turnover with limited genetic contribution from local hunter-gatherers. The succeeding Neolithic population, associated with the Funnel Beaker culture, persisted for only about 1,000 years before immigrants with eastern Steppe-derived ancestry arrived. This second and equally rapid population replacement gave rise to the Single Grave culture with an ancestry profile more similar to present-day Danes. In our multiproxy dataset, these major demographic events are manifested as parallel shifts in genotype, phenotype, diet and land use.

Published
2024

7. Population genomics of post-glacial western Eurasia

Author

Allentoft, Morten E, Sikora, Martin, Refoyo-Martínez, Alba, Irving-Pease, Evan K, Fischer, Anders, Barrie, William, Ingason, Andrés, Stenderup, Jesper, Sjögren, Karl-Göran, Pearson, Alice, Sousa da Mota, Bárbara, Schulz Paulsson, Bettina, Halgren, Alma, Macleod, Ruairidh, Jørkov, Marie Louise Schjellerup, Demeter, Fabrice, Sørensen, Lasse, Nielsen, Poul Otto, Henriksen, Rasmus A, Vimala, Tharsika, McColl, Hugh, Margaryan, Ashot, Ilardo, Melissa, Vaughn, Andrew, Fischer Mortensen, Morten, Nielsen, Anne Birgitte, Ulfeldt Hede, Mikkel, Johannsen, Niels Nørkjær, Rasmussen, Peter, Vinner, Lasse, Renaud, Gabriel, Stern, Aaron, Jensen, Theis Zetner Trolle, Scorrano, Gabriele, Schroeder, Hannes, Lysdahl, Per, Ramsøe, Abigail Daisy, Skorobogatov, Andrei, Schork, Andrew Joseph, Rosengren, Anders, Ruter, Anthony, Outram, Alan, Timoshenko, Aleksey A, Buzhilova, Alexandra, Coppa, Alfredo, Zubova, Alisa, Silva, Ana Maria, Hansen, Anders J, Gromov, Andrey, Logvin, Andrey, Gotfredsen, Anne Birgitte, Henning Nielsen, Bjarne, González-Rabanal, Borja, Lalueza-Fox, Carles, McKenzie, Catriona J, Gaunitz, Charleen, Blasco, Concepción, Liesau, Corina, Martinez-Labarga, Cristina, Pozdnyakov, Dmitri V, Cuenca-Solana, David, Lordkipanidze, David O, En’shin, Dmitri, Salazar-García, Domingo C, Price, T Douglas, Borić, Dušan, Kostyleva, Elena, Veselovskaya, Elizaveta V, Usmanova, Emma R, Cappellini, Enrico, Brinch Petersen, Erik, Kannegaard, Esben, Radina, Francesca, Eylem Yediay, Fulya, Duday, Henri, Gutiérrez-Zugasti, Igor, Merts, Ilya, Potekhina, Inna, Shevnina, Irina, Altinkaya, Isin, Guilaine, Jean, Hansen, Jesper, Aura Tortosa, Joan Emili, Zilhão, João, Vega, Jorge, Buck Pedersen, Kristoffer, Tunia, Krzysztof, Zhao, Lei, Mylnikova, Liudmila N, Larsson, Lars, Metz, Laure, Yepiskoposyan, Levon, Pedersen, Lisbeth, Sarti, Lucia, Orlando, Ludovic, Slimak, Ludovic, Klassen, Lutz, Blank, Malou, González-Morales, Manuel, and Silvestrini, Mara

Subjects
Biological Sciences, Genetics, History, Heritage and Archaeology, Human Society, Historical Studies, Anthropology, Biotechnology, Humans, Genomics, Diploidy, Agriculture, Europe, Metagenomics, General Science & Technology
Abstract

Western Eurasia witnessed several large-scale human migrations during the Holocene1-5. Here, to investigate the cross-continental effects of these migrations, we shotgun-sequenced 317 genomes-mainly from the Mesolithic and Neolithic periods-from across northern and western Eurasia. These were imputed alongside published data to obtain diploid genotypes from more than 1,600 ancient humans. Our analyses revealed a 'great divide' genomic boundary extending from the Black Sea to the Baltic. Mesolithic hunter-gatherers were highly genetically differentiated east and west of this zone, and the effect of the neolithization was equally disparate. Large-scale ancestry shifts occurred in the west as farming was introduced, including near-total replacement of hunter-gatherers in many areas, whereas no substantial ancestry shifts happened east of the zone during the same period. Similarly, relatedness decreased in the west from the Neolithic transition onwards, whereas, east of the Urals, relatedness remained high until around 4,000 BP, consistent with the persistence of localized groups of hunter-gatherers. The boundary dissolved when Yamnaya-related ancestry spread across western Eurasia around 5,000 BP, resulting in a second major turnover that reached most parts of Europe within a 1,000-year span. The genetic origin and fate of the Yamnaya have remained elusive, but we show that hunter-gatherers from the Middle Don region contributed ancestry to them. Yamnaya groups later admixed with individuals associated with the Globular Amphora culture before expanding into Europe. Similar turnovers occurred in western Siberia, where we report new genomic data from a 'Neolithic steppe' cline spanning the Siberian forest steppe to Lake Baikal. These prehistoric migrations had profound and lasting effects on the genetic diversity of Eurasian populations.

Published
2024

8. Imputation of ancient human genomes

Author

Sousa da Mota, Bárbara, Rubinacci, Simone, Cruz Dávalos, Diana Ivette, G. Amorim, Carlos Eduardo, Sikora, Martin, Johannsen, Niels N., Szmyt, Marzena H., Włodarczak, Piotr, Szczepanek, Anita, Przybyła, Marcin M., Schroeder, Hannes, Allentoft, Morten E., Willerslev, Eske, Malaspinas, Anna-Sapfo, and Delaneau, Olivier

Published
2023
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11. Publisher Correction: Population genomics of post-glacial western Eurasia

Author

Allentoft, Morten E., Sikora, Martin, Refoyo-Martínez, Alba, Irving-Pease, Evan K., Fischer, Anders, Barrie, William, Ingason, Andrés, Stenderup, Jesper, Sjögren, Karl-Göran, Pearson, Alice, Sousa da Mota, Bárbara, Schulz Paulsson, Bettina, Halgren, Alma, Macleod, Ruairidh, Jørkov, Marie Louise Schjellerup, Demeter, Fabrice, Sørensen, Lasse, Nielsen, Poul Otto, Henriksen, Rasmus A., Vimala, Tharsika, McColl, Hugh, Margaryan, Ashot, Ilardo, Melissa, Vaughn, Andrew, Fischer Mortensen, Morten, Nielsen, Anne Birgitte, Ulfeldt Hede, Mikkel, Johannsen, Niels Nørkjær, Rasmussen, Peter, Vinner, Lasse, Renaud, Gabriel, Stern, Aaron, Jensen, Theis Zetner Trolle, Scorrano, Gabriele, Schroeder, Hannes, Lysdahl, Per, Ramsøe, Abigail Daisy, Skorobogatov, Andrei, Schork, Andrew Joseph, Rosengren, Anders, Ruter, Anthony, Outram, Alan, Timoshenko, Aleksey A., Buzhilova, Alexandra, Coppa, Alfredo, Zubova, Alisa, Silva, Ana Maria, Hansen, Anders J., Gromov, Andrey, Logvin, Andrey, Gotfredsen, Anne Birgitte, Henning Nielsen, Bjarne, González-Rabanal, Borja, Lalueza-Fox, Carles, McKenzie, Catriona J., Gaunitz, Charleen, Blasco, Concepción, Liesau, Corina, Martinez-Labarga, Cristina, Pozdnyakov, Dmitri V., Cuenca-Solana, David, Lordkipanidze, David O., En’shin, Dmitri, Salazar-García, Domingo C., Price, T. Douglas, Borić, Dušan, Kostyleva, Elena, Veselovskaya, Elizaveta V., Usmanova, Emma R., Cappellini, Enrico, Brinch Petersen, Erik, Kannegaard, Esben, Radina, Francesca, Eylem Yediay, Fulya, Duday, Henri, Gutiérrez-Zugasti, Igor, Merts, Ilya, Potekhina, Inna, Shevnina, Irina, Altinkaya, Isin, Guilaine, Jean, Hansen, Jesper, Aura Tortosa, Joan Emili, Zilhão, João, Vega, Jorge, Buck Pedersen, Kristoffer, Tunia, Krzysztof, Zhao, Lei, Mylnikova, Liudmila N., Larsson, Lars, Metz, Laure, Yepiskoposyan, Levon, Pedersen, Lisbeth, Sarti, Lucia, Orlando, Ludovic, Slimak, Ludovic, Klassen, Lutz, Blank, Malou, González-Morales, Manuel, Silvestrini, Mara, Vretemark, Maria, Nesterova, Marina S., Rykun, Marina, Rolfo, Mario Federico, Szmyt, Marzena, Przybyła, Marcin, Calattini, Mauro, Sablin, Mikhail, Dobisíková, Miluše, Meldgaard, Morten, Johansen, Morten, Berezina, Natalia, Card, Nick, Saveliev, Nikolai A., Poshekhonova, Olga, Rickards, Olga, Lozovskaya, Olga V., Gábor, Olivér, Uldum, Otto Christian, Aurino, Paola, Kosintsev, Pavel, Courtaud, Patrice, Ríos, Patricia, Mortensen, Peder, Lotz, Per, Persson, Per, Bangsgaard, Pernille, de Barros Damgaard, Peter, Vang Petersen, Peter, Martinez, Pilar Prieto, Włodarczak, Piotr, Smolyaninov, Roman V., Maring, Rikke, Menduiña, Roberto, Badalyan, Ruben, Iversen, Rune, Turin, Ruslan, Vasilyev, Sergey, Wåhlin, Sidsel, Borutskaya, Svetlana, Skochina, Svetlana, Sørensen, Søren Anker, Andersen, Søren H., Jørgensen, Thomas, Serikov, Yuri B., Molodin, Vyacheslav I., Smrcka, Vaclav, Merts, Victor, Appadurai, Vivek, Moiseyev, Vyacheslav, Magnusson, Yvonne, Kjær, Kurt H., Lynnerup, Niels, Lawson, Daniel J., Sudmant, Peter H., Rasmussen, Simon, Korneliussen, Thorfinn Sand, Durbin, Richard, Nielsen, Rasmus, Delaneau, Olivier, Werge, Thomas, Racimo, Fernando, Kristiansen, Kristian, and Willerslev, Eske

Published
2024
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12. A 2-million-year-old ecosystem in Greenland uncovered by environmental DNA

Author

Kjær, Kurt H., Winther Pedersen, Mikkel, De Sanctis, Bianca, De Cahsan, Binia, Korneliussen, Thorfinn S., Michelsen, Christian S., Sand, Karina K., Jelavić, Stanislav, Ruter, Anthony H., Schmidt, Astrid M. A., Kjeldsen, Kristian K., Tesakov, Alexey S., Snowball, Ian, Gosse, John C., Alsos, Inger G., Wang, Yucheng, Dockter, Christoph, Rasmussen, Magnus, Jørgensen, Morten E., Skadhauge, Birgitte, Prohaska, Ana, Kristensen, Jeppe Å., Bjerager, Morten, Allentoft, Morten E., Coissac, Eric, Rouillard, Alexandra, Simakova, Alexandra, Fernandez-Guerra, Antonio, Bowler, Chris, Macias-Fauria, Marc, Vinner, Lasse, Welch, John J., Hidy, Alan J., Sikora, Martin, Collins, Matthew J., Durbin, Richard, Larsen, Nicolaj K., and Willerslev, Eske

Published
2022
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13. Aquatic environmental DNA: A review of the macro-organismal biomonitoring revolution

Author

Takahashi, Miwa, Saccò, Mattia, Kestel, Joshua H., Nester, Georgia, Campbell, Matthew A., van der Heyde, Mieke, Heydenrych, Matthew J., Juszkiewicz, David J., Nevill, Paul, Dawkins, Kathryn L., Bessey, Cindy, Fernandes, Kristen, Miller, Haylea, Power, Matthew, Mousavi-Derazmahalleh, Mahsa, Newton, Joshua P., White, Nicole E., Richards, Zoe T., and Allentoft, Morten E.

Published
2023
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15. Genomic ancestry, diet and microbiomes of Upper Palaeolithic hunter-gatherers from San Teodoro cave

Author

Scorrano, Gabriele, Nielsen, Sofie Holtsmark, Vetro, Domenico Lo, Sawafuji, Rikai, Mackie, Meaghan, Margaryan, Ashot, Fotakis, Anna K., Martínez-Labarga, Cristina, Fabbri, Pier Francesco, Allentoft, Morten E., Carra, Marialetizia, Martini, Fabio, Rickards, Olga, Olsen, Jesper V., Pedersen, Mikkel Winther, Cappellini, Enrico, and Sikora, Martin

Published
2022
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17. Towards predicting the geographical origin of ancient samples with metagenomic data.

Author

Bozzi, Davide, Neuenschwander, Samuel, Cruz Dávalos, Diana Ivette, Sousa da Mota, Bárbara, Schroeder, Hannes, Moreno-Mayar, J. Víctor, Allentoft, Morten E., and Malaspinas, Anna-Sapfo

Abstract

Reconstructing the history—such as the place of birth and death—of an individual sample is a fundamental goal in ancient DNA (aDNA) studies. However, knowing the place of death can be particularly challenging when samples come from museum collections with incomplete or erroneous archives. While analyses of human DNA and isotope data can inform us about the ancestry of an individual and provide clues about where the person lived, they cannot specifically trace the place of death. Moreover, while ancient human DNA can be retrieved, a large fraction of the sequenced molecules in ancient DNA studies derive from exogenous DNA. This DNA—which is usually discarded in aDNA analyses—is constituted mostly by microbial DNA from soil-dwelling microorganisms that have colonized the buried remains post-mortem. In this study, we hypothesize that remains of individuals buried in the same or close geographic areas, exposed to similar microbial communities, could harbor more similar metagenomes. We propose to use metagenomic data from ancient samples' shotgun sequencing to locate the place of death of a given individual which can also help to solve cases of sample mislabeling. We used a k-mer-based approach to compute similarity scores between metagenomic samples from different locations and propose a method based on dimensionality reduction and logistic regression to assign a geographical origin to target samples. We apply our method to several public datasets and observe that individual samples from closer geographic locations tend to show higher similarities in their metagenomes compared to those of different origin, allowing good geographical predictions of test samples. Moreover, we observe that the genus Streptomyces commonly infiltrates ancient remains and represents a valuable biomarker to trace the samples' geographic origin. Our results provide a proof of concept and show how metagenomic data can also be used to shed light on the place of origin of ancient samples. [ABSTRACT FROM AUTHOR]

Published
2024
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19. Steppe Ancestry in western Eurasia and the spread of the Germanic Languages

Author

McColl, Hugh, primary, Kroonen, Guus, additional, Moreno-Mayar, J. Víctor, additional, Valeur Seersholm, Frederik, additional, Scorrano, Gabriele, additional, Pinotti, Thomaz, additional, Vimala, Tharsika, additional, Sindbæk, Søren M., additional, Ethelberg, Per, additional, Fyfe, Ralph, additional, Gaillard, Marie-José, additional, Larsen, Hanne M. Ellegård, additional, Mortensen, Morten Fischer, additional, Demeter, Fabrice, additional, Jørkov, Marie Louise S., additional, Bergerbrant, Sophie, additional, Damgaard, Peter de Barros, additional, Allentoft, Morten E., additional, Vinner, Lasse, additional, Gaunitz, Charleen, additional, Ramsøe, Abigail, additional, Altinkaya, Isin, additional, Amund Henriksen, Rasmus, additional, Irving-Pease, Evan K., additional, Sabatini, Serena, additional, Fischer, Anders, additional, Barrie, William, additional, Ingason, Andrés, additional, Rosengren, Anders, additional, Vaughn, Andrew, additional, Cao, Jialu, additional, Staring, Jacqueline, additional, Stenderup, Jesper, additional, Yediay, Fulya Eylem, additional, Ahlström, Torbjörn, additional, Albris, Sofie Laurine, additional, Atabiev, Biyaslan, additional, Bangsgaard, Pernille, additional, Belcastro, Maria Giovanna, additional, Card, Nick, additional, Charlier, Philippe, additional, Chernykh, Elizaveta, additional, Christiansen, Torben Trier, additional, Coppa, Alfredo, additional, De Coster, Maura, additional, Denham, Sean Dexter, additional, Desenne, Sophie, additional, Downes, Jane, additional, Frei, Karin Margarita, additional, Gábor, Olivér, additional, Gårdsvoll, Johan Zakarias, additional, Glørstad, Zanette Tsigaridas, additional, Hansen, Jesper, additional, Heeren, Stijn, additional, Henriksen, Merete, additional, Heyd, Volker, additional, Høj, Mette, additional, Holst, Mads Kähler, additional, Jankauskas, Rimantas, additional, Janson, Henrik, additional, Jessen, Mads Dengsø, additional, Johannsen, Jens Winther, additional, Johansen, Torkel, additional, Kastholm, Ole Thirup, additional, Kern, Anton, additional, Khaskhanov, Ruslan, additional, Kjær, Katrine, additional, Kolosov, Vladimir, additional, Kootker, Lisette M., additional, Larsen, Anne Christine, additional, Lejars, Thierry, additional, Løvschal, Mette, additional, Lynnerup, Niels, additional, Magnusson, Yvonne, additional, Mannermaa, Kristiina, additional, Masyakin, Vyacheslav, additional, Melheim, Anne Lene, additional, Merkyte, Inga, additional, Moiseyev, Vyacheslav, additional, Møller, Stig Bergmann, additional, Molnár, Erika, additional, Mortensen, Nadja, additional, Murphy, Eileen, additional, Nielsen, Bjarne Henning, additional, Pany-Kucera, Doris, additional, Paulsson, Bettina Schulz, additional, Ponce de León, Marcia S, additional, Reiersen, Håkon, additional, Reinhard, Walter, additional, Sajantila, Antti, additional, Skar, Birgitte, additional, Slavchev, Vladimir, additional, Smrčka, Václav, additional, Sørensen, Lasse, additional, Tiefengraber, Georg, additional, Uldum, Otto Christian, additional, Vega, Jorge, additional, Vitali, Daniele, additional, Voloshinov, Alexey, additional, Wåhlin, Sidsel, additional, Wendling, Holger, additional, Wessman, Anna, additional, Wilhelmson, Helene, additional, Wiltschke, Karin, additional, Zilhao, João, additional, Zollikofer, Christoph PE, additional, Sand Korneliussen, Thorfinn, additional, Chaume, Bruno, additional, Demoule, Jean-Paul, additional, Werge, Thomas, additional, Olsen, Line, additional, Nielsen, Rasmus, additional, Hedeager, Lotte, additional, Kristiansen, Kristian, additional, Sikora, Martin, additional, and Willerslev, Eske, additional

Published
2024
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20. Publisher Correction: Population genomics of post-glacial western Eurasia (Nature, (2024), 625, 7994, (301-311), 10.1038/s41586-023-06865-0)

Author

Allentoft, Morten, Sikora, Martin, Refoyo-Martínez, Alba, Irving-Pease, Evan, Fischer, Anders, Barrie, William, Ingason, Andrés, Stenderup, Jesper, Sjögren, Karl-Göran, Pearson, Alice, Sousa da Mota, Bárbara, Schulz Paulsson, Bettina, Halgren, Alma, Macleod, Ruairidh, Schjellerup Jørkov, Marie Louise, Demeter, Fabrice, Sørensen, Lasse, Nielsen, Poul Otto, Henriksen, Rasmus, Vimala, Tharsika, McColl, Hugh, Margaryan, Ashot, Ilardo, Melissa, Vaughn, Andrew, Mortensen, Morten Fischer, Nielsen, Anne Birgitte, Ulfeldt Hede, Mikkel, Johannsen, Niels Nørkjær, Rasmussen, Peter, Vinner, Lasse, Renaud, Gabriel, Stern, Aaron, Jensen, Theis, Scorrano, Gabriele, Schroeder, Hannes, Lysdahl, Per, Ramsøe, Abigail Daisy, Skorobogatov, Andrei, Schork, Andrew Joseph, Rosengren, Anders, Ruter, Anthony, Outram, Alan, Timoshenko, Aleksey, Buzhilova, Alexandra, Coppa, Alfredo, Zubova, Alisa, Silva, Ana Maria, Hansen, Anders, Gromov, Andrey, Logvin, Andrey, Gotfredsen, Anne Birgitte, Nielsen, Bjarne Henning, González-Rabanal, Borja, Lalueza-Fox, Carles, McKenzie, Catriona, Gaunitz, Charleen, Blasco, Concepción, Liesau, Corina, Martinez-Labarga, Cristina, Pozdnyakov, Dmitri, Cuenca-Solana, David, Lordkipanidze, David, En’shin, Dmitri, Salazar-García, Domingo, Price, Douglas, Borić, Dušan, Kostyleva, Elena, Veselovskaya, Elizaveta, Usmanova, Emma, Cappellini, Enrico, Petersen, Erik Brinch, Kannegaard, Esben, Radina, Francesca, Yediay, Fulya Eylem, Duday, Henri, Gutiérrez-Zugasti, Igor, Merts, Ilya, Potekhina, Inna, Shevnina, Irina, Altinkaya, Isin, Guilaine, Jean, Hansen, Jesper, Aura Tortosa, Joan Emili, Zilhão, João, Vega, Jorge, Buck Pedersen, Kristoffer, Tunia, Krzysztof, Zhao, Lei, Mylnikova, Liudmila, Larsson, Lars, Metz, Laure, Yepiskoposyan, Levon, Pedersen, Lisbeth, Sarti, Lucia, Orlando, Ludovic, Slimak, Ludovic, Klassen, Lutz, Blank, Malou, González-Morales, Manuel, Silvestrini, Mara, Vretemark, Maria, Nesterova, Marina, Rykun, Marina, Rolfo, Mario Federico, Szmyt, Marzena, Przybyła, Marcin, Calattini, Mauro, Sablin, Mikhail, Dobisíková, Miluše, Meldgaard, Morten, Johansen, Morten, Berezina, Natalia, Card, Nick, Saveliev, Nikolai, Poshekhonova, Olga, Rickards, Olga, Lozovskaya, Olga, Gábor, Olivér, Uldum, Otto Christian, Aurino, Paola, Kosintsev, Pavel, Courtaud, Patrice, Ríos, Patricia, Mortensen, Peder, Lotz, Per, Persson, Per, Bangsgaard, Pernille, Damgaard, Peter de Barros, Petersen, Peter Vang, Prieto Martínez, Pilar, Włodarczak, Piotr, Smolyaninov, Roman, Maring, Rikke, Menduiña, Roberto, Badalyan, Ruben, Iversen, Rune, Turin, Ruslan, Vasilyev, Sergey, Wåhlin, Sidsel, Borutskaya, Svetlana, Skochina, Svetlana, Sørensen, Søren Anker, Andersen, Søren, Jørgensen, Thomas, Serikov, Yuri, Molodin, Vyacheslav, Smrcka, Vaclav, Merts, Victor, Appadurai, Vivek, Moiseyev, Vyacheslav, Magnusson,Yvonne, Kjær, Kurt, Lynnerup, Niels, Lawson, Daniel, Sudmant, Peter, Rasmussen, Simon, Korneliussen, Thorfinn Sand, Durbin, Richard, Nielsen, Rasmus, Delanea, Olivier, Werge, Thomas, Racimo, Fernando, Kristiansen, Kristian, Willerslev, Eske, Allentoft, Morten, Sikora, Martin, Refoyo-Martínez, Alba, Irving-Pease, Evan, Fischer, Anders, Barrie, William, Ingason, Andrés, Stenderup, Jesper, Sjögren, Karl-Göran, Pearson, Alice, Sousa da Mota, Bárbara, Schulz Paulsson, Bettina, Halgren, Alma, Macleod, Ruairidh, Schjellerup Jørkov, Marie Louise, Demeter, Fabrice, Sørensen, Lasse, Nielsen, Poul Otto, Henriksen, Rasmus, Vimala, Tharsika, McColl, Hugh, Margaryan, Ashot, Ilardo, Melissa, Vaughn, Andrew, Mortensen, Morten Fischer, Nielsen, Anne Birgitte, Ulfeldt Hede, Mikkel, Johannsen, Niels Nørkjær, Rasmussen, Peter, Vinner, Lasse, Renaud, Gabriel, Stern, Aaron, Jensen, Theis, Scorrano, Gabriele, Schroeder, Hannes, Lysdahl, Per, Ramsøe, Abigail Daisy, Skorobogatov, Andrei, Schork, Andrew Joseph, Rosengren, Anders, Ruter, Anthony, Outram, Alan, Timoshenko, Aleksey, Buzhilova, Alexandra, Coppa, Alfredo, Zubova, Alisa, Silva, Ana Maria, Hansen, Anders, Gromov, Andrey, Logvin, Andrey, Gotfredsen, Anne Birgitte, Nielsen, Bjarne Henning, González-Rabanal, Borja, Lalueza-Fox, Carles, McKenzie, Catriona, Gaunitz, Charleen, Blasco, Concepción, Liesau, Corina, Martinez-Labarga, Cristina, Pozdnyakov, Dmitri, Cuenca-Solana, David, Lordkipanidze, David, En’shin, Dmitri, Salazar-García, Domingo, Price, Douglas, Borić, Dušan, Kostyleva, Elena, Veselovskaya, Elizaveta, Usmanova, Emma, Cappellini, Enrico, Petersen, Erik Brinch, Kannegaard, Esben, Radina, Francesca, Yediay, Fulya Eylem, Duday, Henri, Gutiérrez-Zugasti, Igor, Merts, Ilya, Potekhina, Inna, Shevnina, Irina, Altinkaya, Isin, Guilaine, Jean, Hansen, Jesper, Aura Tortosa, Joan Emili, Zilhão, João, Vega, Jorge, Buck Pedersen, Kristoffer, Tunia, Krzysztof, Zhao, Lei, Mylnikova, Liudmila, Larsson, Lars, Metz, Laure, Yepiskoposyan, Levon, Pedersen, Lisbeth, Sarti, Lucia, Orlando, Ludovic, Slimak, Ludovic, Klassen, Lutz, Blank, Malou, González-Morales, Manuel, Silvestrini, Mara, Vretemark, Maria, Nesterova, Marina, Rykun, Marina, Rolfo, Mario Federico, Szmyt, Marzena, Przybyła, Marcin, Calattini, Mauro, Sablin, Mikhail, Dobisíková, Miluše, Meldgaard, Morten, Johansen, Morten, Berezina, Natalia, Card, Nick, Saveliev, Nikolai, Poshekhonova, Olga, Rickards, Olga, Lozovskaya, Olga, Gábor, Olivér, Uldum, Otto Christian, Aurino, Paola, Kosintsev, Pavel, Courtaud, Patrice, Ríos, Patricia, Mortensen, Peder, Lotz, Per, Persson, Per, Bangsgaard, Pernille, Damgaard, Peter de Barros, Petersen, Peter Vang, Prieto Martínez, Pilar, Włodarczak, Piotr, Smolyaninov, Roman, Maring, Rikke, Menduiña, Roberto, Badalyan, Ruben, Iversen, Rune, Turin, Ruslan, Vasilyev, Sergey, Wåhlin, Sidsel, Borutskaya, Svetlana, Skochina, Svetlana, Sørensen, Søren Anker, Andersen, Søren, Jørgensen, Thomas, Serikov, Yuri, Molodin, Vyacheslav, Smrcka, Vaclav, Merts, Victor, Appadurai, Vivek, Moiseyev, Vyacheslav, Magnusson,Yvonne, Kjær, Kurt, Lynnerup, Niels, Lawson, Daniel, Sudmant, Peter, Rasmussen, Simon, Korneliussen, Thorfinn Sand, Durbin, Richard, Nielsen, Rasmus, Delanea, Olivier, Werge, Thomas, Racimo, Fernando, Kristiansen, Kristian, and Willerslev, Eske

Abstract

In the version of this article initially published, there were errors in the second affiliations for Levon Yepiskoposyan (Russian-Armenian University, Yerevan, Armenia) and Sergey Vasilyev (Center for Egyptological Studies, Russian Academy of Sciences, Moscow, Russian Federation), and in the first affiliation for Ruben Badalyan (Institute of Archaeology and Ethnography, National Academy of Sciences, Yerevan, Armenia); the affiliations are amended in the HTML and PDF versions of the article.

Published
2024

21. Population genomics of post-glacial western Eurasia

Author

Allentoft, Morten E., Sikora, Martin, Refoyo-Martínez, Alba, Irving-Pease, Evan K., Fischer, Anders, Barrie, William, Ingason, Andrés, Stenderup, Jesper, Sjögren, Karl Göran, Pearson, Alice, Sousa da Mota, Bárbara, Schulz Paulsson, Bettina, Halgren, Alma, Macleod, Ruairidh, Jørkov, Marie Louise Schjellerup, Demeter, Fabrice, Sørensen, Lasse, Nielsen, Poul Otto, Henriksen, Rasmus A., Vimala, Tharsika, McColl, Hugh, Margaryan, Ashot, Ilardo, Melissa, Vaughn, Andrew, Fischer Mortensen, Morten, Nielsen, Anne Birgitte, Ulfeldt Hede, Mikkel, Johannsen, Niels Nørkjær, Rasmussen, Peter, Vinner, Lasse, Renaud, Gabriel, Stern, Aaron, Jensen, Theis Zetner Trolle, Scorrano, Gabriele, Schroeder, Hannes, Lysdahl, Per, Ramsøe, Abigail Daisy, Skorobogatov, Andrei, Schork, Andrew Joseph, Rosengren, Anders, Ruter, Anthony, Outram, Alan, Timoshenko, Aleksey A., Buzhilova, Alexandra, Coppa, Alfredo, Zubova, Alisa, Silva, Ana Maria, Hansen, Anders J., Gromov, Andrey, Logvin, Andrey, Gotfredsen, Anne Birgitte, Henning Nielsen, Bjarne, González-Rabanal, Borja, Lalueza-Fox, Carles, McKenzie, Catriona J., Gaunitz, Charleen, Blasco, Concepción, Liesau, Corina, Martinez-Labarga, Cristina, Pozdnyakov, Dmitri V., Cuenca-Solana, David, Lordkipanidze, David O., En’shin, Dmitri, Salazar-García, Domingo C., Price, T. Douglas, Borić, Dušan, Kostyleva, Elena, Veselovskaya, Elizaveta V., Usmanova, Emma R., Cappellini, Enrico, Brinch Petersen, Erik, Kannegaard, Esben, Radina, Francesca, Eylem Yediay, Fulya, Duday, Henri, Gutiérrez-Zugasti, Igor, Merts, Ilya, Potekhina, Inna, Shevnina, Irina, Altinkaya, Isin, Guilaine, Jean, Hansen, Jesper, Aura Tortosa, Joan Emili, Zilhão, João, Vega, Jorge, Buck Pedersen, Kristoffer, Tunia, Krzysztof, Zhao, Lei, Mylnikova, Liudmila N., Larsson, Lars, Metz, Laure, Yepiskoposyan, Levon, Pedersen, Lisbeth, Sarti, Lucia, Orlando, Ludovic, Slimak, Ludovic, Klassen, Lutz, Blank, Malou, González-Morales, Manuel, Silvestrini, Mara, Vretemark, Maria, Nesterova, Marina S., Rykun, Marina, Rolfo, Mario Federico, Szmyt, Marzena, Przybyła, Marcin, Calattini, Mauro, Sablin, Mikhail, Dobisíková, Miluše, Meldgaard, Morten, Johansen, Morten, Berezina, Natalia, Card, Nick, Saveliev, Nikolai A., Poshekhonova, Olga, Rickards, Olga, Lozovskaya, Olga V., Gábor, Olivér, Uldum, Otto Christian, Aurino, Paola, Kosintsev, Pavel, Courtaud, Patrice, Ríos, Patricia, Mortensen, Peder, Lotz, Per, Persson, Per, Bangsgaard, Pernille, de Barros Damgaard, Peter, Vang Petersen, Peter, Martinez, Pilar Prieto, Włodarczak, Piotr, Smolyaninov, Roman V., Maring, Rikke, Menduiña, Roberto, Badalyan, Ruben, Iversen, Rune, Turin, Ruslan, Vasilyev, Sergey, Wåhlin, Sidsel, Borutskaya, Svetlana, Skochina, Svetlana, Sørensen, Søren Anker, Andersen, Søren H., Jørgensen, Thomas, Serikov, Yuri B., Molodin, Vyacheslav I., Smrcka, Vaclav, Merts, Victor, Appadurai, Vivek, Moiseyev, Vyacheslav, Magnusson, Yvonne, Kjær, Kurt H., Lynnerup, Niels, Lawson, Daniel J., Sudmant, Peter H., Rasmussen, Simon, Korneliussen, Thorfinn Sand, Durbin, Richard, Nielsen, Rasmus, Delaneau, Olivier, Werge, Thomas, Racimo, Fernando, Kristiansen, Kristian, Willerslev, Eske, Allentoft, Morten E., Sikora, Martin, Refoyo-Martínez, Alba, Irving-Pease, Evan K., Fischer, Anders, Barrie, William, Ingason, Andrés, Stenderup, Jesper, Sjögren, Karl Göran, Pearson, Alice, Sousa da Mota, Bárbara, Schulz Paulsson, Bettina, Halgren, Alma, Macleod, Ruairidh, Jørkov, Marie Louise Schjellerup, Demeter, Fabrice, Sørensen, Lasse, Nielsen, Poul Otto, Henriksen, Rasmus A., Vimala, Tharsika, McColl, Hugh, Margaryan, Ashot, Ilardo, Melissa, Vaughn, Andrew, Fischer Mortensen, Morten, Nielsen, Anne Birgitte, Ulfeldt Hede, Mikkel, Johannsen, Niels Nørkjær, Rasmussen, Peter, Vinner, Lasse, Renaud, Gabriel, Stern, Aaron, Jensen, Theis Zetner Trolle, Scorrano, Gabriele, Schroeder, Hannes, Lysdahl, Per, Ramsøe, Abigail Daisy, Skorobogatov, Andrei, Schork, Andrew Joseph, Rosengren, Anders, Ruter, Anthony, Outram, Alan, Timoshenko, Aleksey A., Buzhilova, Alexandra, Coppa, Alfredo, Zubova, Alisa, Silva, Ana Maria, Hansen, Anders J., Gromov, Andrey, Logvin, Andrey, Gotfredsen, Anne Birgitte, Henning Nielsen, Bjarne, González-Rabanal, Borja, Lalueza-Fox, Carles, McKenzie, Catriona J., Gaunitz, Charleen, Blasco, Concepción, Liesau, Corina, Martinez-Labarga, Cristina, Pozdnyakov, Dmitri V., Cuenca-Solana, David, Lordkipanidze, David O., En’shin, Dmitri, Salazar-García, Domingo C., Price, T. Douglas, Borić, Dušan, Kostyleva, Elena, Veselovskaya, Elizaveta V., Usmanova, Emma R., Cappellini, Enrico, Brinch Petersen, Erik, Kannegaard, Esben, Radina, Francesca, Eylem Yediay, Fulya, Duday, Henri, Gutiérrez-Zugasti, Igor, Merts, Ilya, Potekhina, Inna, Shevnina, Irina, Altinkaya, Isin, Guilaine, Jean, Hansen, Jesper, Aura Tortosa, Joan Emili, Zilhão, João, Vega, Jorge, Buck Pedersen, Kristoffer, Tunia, Krzysztof, Zhao, Lei, Mylnikova, Liudmila N., Larsson, Lars, Metz, Laure, Yepiskoposyan, Levon, Pedersen, Lisbeth, Sarti, Lucia, Orlando, Ludovic, Slimak, Ludovic, Klassen, Lutz, Blank, Malou, González-Morales, Manuel, Silvestrini, Mara, Vretemark, Maria, Nesterova, Marina S., Rykun, Marina, Rolfo, Mario Federico, Szmyt, Marzena, Przybyła, Marcin, Calattini, Mauro, Sablin, Mikhail, Dobisíková, Miluše, Meldgaard, Morten, Johansen, Morten, Berezina, Natalia, Card, Nick, Saveliev, Nikolai A., Poshekhonova, Olga, Rickards, Olga, Lozovskaya, Olga V., Gábor, Olivér, Uldum, Otto Christian, Aurino, Paola, Kosintsev, Pavel, Courtaud, Patrice, Ríos, Patricia, Mortensen, Peder, Lotz, Per, Persson, Per, Bangsgaard, Pernille, de Barros Damgaard, Peter, Vang Petersen, Peter, Martinez, Pilar Prieto, Włodarczak, Piotr, Smolyaninov, Roman V., Maring, Rikke, Menduiña, Roberto, Badalyan, Ruben, Iversen, Rune, Turin, Ruslan, Vasilyev, Sergey, Wåhlin, Sidsel, Borutskaya, Svetlana, Skochina, Svetlana, Sørensen, Søren Anker, Andersen, Søren H., Jørgensen, Thomas, Serikov, Yuri B., Molodin, Vyacheslav I., Smrcka, Vaclav, Merts, Victor, Appadurai, Vivek, Moiseyev, Vyacheslav, Magnusson, Yvonne, Kjær, Kurt H., Lynnerup, Niels, Lawson, Daniel J., Sudmant, Peter H., Rasmussen, Simon, Korneliussen, Thorfinn Sand, Durbin, Richard, Nielsen, Rasmus, Delaneau, Olivier, Werge, Thomas, Racimo, Fernando, Kristiansen, Kristian, and Willerslev, Eske

Abstract

Western Eurasia witnessed several large-scale human migrations during the Holocene1–5. Here, to investigate the cross-continental effects of these migrations, we shotgun-sequenced 317 genomes—mainly from the Mesolithic and Neolithic periods—from across northern and western Eurasia. These were imputed alongside published data to obtain diploid genotypes from more than 1,600 ancient humans. Our analyses revealed a ‘great divide’ genomic boundary extending from the Black Sea to the Baltic. Mesolithic hunter-gatherers were highly genetically differentiated east and west of this zone, and the effect of the neolithization was equally disparate. Large-scale ancestry shifts occurred in the west as farming was introduced, including near-total replacement of hunter-gatherers in many areas, whereas no substantial ancestry shifts happened east of the zone during the same period. Similarly, relatedness decreased in the west from the Neolithic transition onwards, whereas, east of the Urals, relatedness remained high until around 4,000 bp, consistent with the persistence of localized groups of hunter-gatherers. The boundary dissolved when Yamnaya-related ancestry spread across western Eurasia around 5,000 bp, resulting in a second major turnover that reached most parts of Europe within a 1,000-year span. The genetic origin and fate of the Yamnaya have remained elusive, but we show that hunter-gatherers from the Middle Don region contributed ancestry to them. Yamnaya groups later admixed with individuals associated with the Globular Amphora culture before expanding into Europe. Similar turnovers occurred in western Siberia, where we report new genomic data from a ‘Neolithic steppe’ cline spanning the Siberian forest steppe to Lake Baikal. These prehistoric migrations had profound and lasting effects on the genetic diversity of Eurasian populations.

Published
2024

22. 100 ancient genomes show repeated population turnovers in Neolithic Denmark

Author

Allentoft, Morten E., Sikora, Martin, Fischer, Anders, Sjögren, Karl Göran, Ingason, Andrés, Macleod, Ruairidh, Rosengren, Anders, Schulz Paulsson, Bettina, Jørkov, Marie Louise Schjellerup, Novosolov, Maria, Stenderup, Jesper, Price, T. Douglas, Fischer Mortensen, Morten, Nielsen, Anne Birgitte, Ulfeldt Hede, Mikkel, Sørensen, Lasse, Nielsen, Poul Otto, Rasmussen, Peter, Jensen, Theis Zetner Trolle, Refoyo-Martínez, Alba, Irving-Pease, Evan K., Barrie, William, Pearson, Alice, Sousa da Mota, Bárbara, Demeter, Fabrice, Henriksen, Rasmus A., Vimala, Tharsika, McColl, Hugh, Vaughn, Andrew, Vinner, Lasse, Renaud, Gabriel, Stern, Aaron, Johannsen, Niels Nørkjær, Ramsøe, Abigail Daisy, Schork, Andrew Joseph, Ruter, Anthony, Gotfredsen, Anne Birgitte, Henning Nielsen, Bjarne, Brinch Petersen, Erik, Kannegaard, Esben, Hansen, Jesper, Buck Pedersen, Kristoffer, Pedersen, Lisbeth, Klassen, Lutz, Meldgaard, Morten, Johansen, Morten, Uldum, Otto Christian, Lotz, Per, Lysdahl, Per, Bangsgaard, Pernille, Petersen, Peter Vang, Maring, Rikke, Iversen, Rune, Wåhlin, Sidsel, Anker Sørensen, Søren, Andersen, Søren H., Jørgensen, Thomas, Lynnerup, Niels, Lawson, Daniel J., Rasmussen, Simon, Korneliussen, Thorfinn Sand, Kjær, Kurt H., Durbin, Richard, Nielsen, Rasmus, Delaneau, Olivier, Werge, Thomas, Kristiansen, Kristian, Willerslev, Eske, Allentoft, Morten E., Sikora, Martin, Fischer, Anders, Sjögren, Karl Göran, Ingason, Andrés, Macleod, Ruairidh, Rosengren, Anders, Schulz Paulsson, Bettina, Jørkov, Marie Louise Schjellerup, Novosolov, Maria, Stenderup, Jesper, Price, T. Douglas, Fischer Mortensen, Morten, Nielsen, Anne Birgitte, Ulfeldt Hede, Mikkel, Sørensen, Lasse, Nielsen, Poul Otto, Rasmussen, Peter, Jensen, Theis Zetner Trolle, Refoyo-Martínez, Alba, Irving-Pease, Evan K., Barrie, William, Pearson, Alice, Sousa da Mota, Bárbara, Demeter, Fabrice, Henriksen, Rasmus A., Vimala, Tharsika, McColl, Hugh, Vaughn, Andrew, Vinner, Lasse, Renaud, Gabriel, Stern, Aaron, Johannsen, Niels Nørkjær, Ramsøe, Abigail Daisy, Schork, Andrew Joseph, Ruter, Anthony, Gotfredsen, Anne Birgitte, Henning Nielsen, Bjarne, Brinch Petersen, Erik, Kannegaard, Esben, Hansen, Jesper, Buck Pedersen, Kristoffer, Pedersen, Lisbeth, Klassen, Lutz, Meldgaard, Morten, Johansen, Morten, Uldum, Otto Christian, Lotz, Per, Lysdahl, Per, Bangsgaard, Pernille, Petersen, Peter Vang, Maring, Rikke, Iversen, Rune, Wåhlin, Sidsel, Anker Sørensen, Søren, Andersen, Søren H., Jørgensen, Thomas, Lynnerup, Niels, Lawson, Daniel J., Rasmussen, Simon, Korneliussen, Thorfinn Sand, Kjær, Kurt H., Durbin, Richard, Nielsen, Rasmus, Delaneau, Olivier, Werge, Thomas, Kristiansen, Kristian, and Willerslev, Eske

Abstract

Major migration events in Holocene Eurasia have been characterized genetically at broad regional scales1–4. However, insights into the population dynamics in the contact zones are hampered by a lack of ancient genomic data sampled at high spatiotemporal resolution5–7. Here, to address this, we analysed shotgun-sequenced genomes from 100 skeletons spanning 7,300 years of the Mesolithic period, Neolithic period and Early Bronze Age in Denmark and integrated these with proxies for diet (13C and 15N content), mobility (87Sr/86Sr ratio) and vegetation cover (pollen). We observe that Danish Mesolithic individuals of the Maglemose, Kongemose and Ertebølle cultures form a distinct genetic cluster related to other Western European hunter-gatherers. Despite shifts in material culture they displayed genetic homogeneity from around 10,500 to 5,900 calibrated years before present, when Neolithic farmers with Anatolian-derived ancestry arrived. Although the Neolithic transition was delayed by more than a millennium relative to Central Europe, it was very abrupt and resulted in a population turnover with limited genetic contribution from local hunter-gatherers. The succeeding Neolithic population, associated with the Funnel Beaker culture, persisted for only about 1,000 years before immigrants with eastern Steppe-derived ancestry arrived. This second and equally rapid population replacement gave rise to the Single Grave culture with an ancestry profile more similar to present-day Danes. In our multiproxy dataset, these major demographic events are manifested as parallel shifts in genotype, phenotype, diet and land use.

Published
2024

23. Population genomics of post-glacial western Eurasia

Author

Lundbeck Foundation, Novo Nordisk Foundation, Wellcome Trust, Carlsberg Foundation, Danish National Research Foundation, University of Copenhagen, Ferring Pharmaceuticals, Swedish Foundation for Humanities and Social Sciences, Villum Fonden, Independent Research Fund Denmark, Hanne and Torkel Weis-Fogh Fund, Wellcome, Swiss National Science Foundation, European Research Council, Aarhus University Research Foundation, Ministry of Education and Science (Kazakhstan), Ministerio de Economía y Competitividad (España), Ministero dell'Istruzione, dell'Università e della Ricerca, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Generalitat Valenciana, Nomis Foundation, European Commission, Ministry of Science and Higher Education of the Russian Federation, Ministry of Education, Science, Culture and Sports of the Republic of Armenia, National Institute of General Medical Sciences (US), Sikora, Martin [0000-0003-2818-8319], Lalueza-Fox, Carles [0000-0002-1730-5914], Allentoft, Morten, Sikora, Martin, Refoyo-Martínez, Alba, Irving-Pease, Evan K., Fischer, Anders, Barrie, William, Ingason, Andrés, Stenderup, Jesper, Sjögren, Karl-Göran, Pearson, Alice, Mota, Bárbara Sousa da, Schulz Paulsson, Bettina, Halgren, Alma, Macleod, Ruairidh, Jørkov, Marie Louise S., Demeter, Fabrice, Sørensen, Lasse, Nielsen, Poul Otto, Henriksen, Rasmus A., Vimala, Tharsika, McColl, Hugh, Margaryan, Ashot, Ilardo, Melissa, Vaughn, Andrew, Fischer Mortensen, Morten, Nielsen, Anne Birgitte, Ulfeldt Hede, Mikkel, Johannsen, Niels Nørkjær, Rasmussen, Peter, Vinner, Lasse, Renaud, Gabriel, Stern, Aaron, Trolle Jensen, Theis Zetner, Scorrano, Gabriele, Schroeder, Hannes, Lysdahl, Per, Ramsøe, Abigail Daisy, Skorobogatov, Andrei, Schork, Andrew Joseph, Rosengren, Anders, Ruter, Anthony, Outram, Alan, Timoshenko, Aleksey A., Buzhilova, Alexandra, Coppa, Alfredo, Zubova, Alisa, Silva, Ana María, Hansen, Anders J., Gromov, Andrey, Logvin, Andrey, Gotfredsen, Anne Birgitte, Nielsen, Bjarne Henning, González-Rabanal, Borja, Lalueza-Fox, Carles, McKenzie, Catriona J., Gaunitz, Charleen, Blasco, Concepción, Liesau, Corina, Martínez-Labarga, Cristina, Pozdnyakov, Dmitri V., Cuenca-Solana, David, Lordkipanidze, David O., En’shin, Dmitri, Salazar García, Domingo Carlos, Price, T. Douglas, Borić, Dušan, Kostyleva, Elena, Veselovskaya, Elizaveta V., Usmanova, Emma R., Cappellini, Enrico, Petersen, Erik Brinch, Kannegaard, Esben, Radina, Francesca, Yediay, Fulya Eylem, Duday, Henri, Gutiérrez-Zugasti, Igor, Merts, Ilya, Potekhina, Inna, Shevnina, Irina, Altinkaya, Isin, Guilaine, Jean, Hansen, Jesper, Aura Tortosa, Joan Emili, Zilhão, João, Vega, Jorge, Pedersen, Kristoffer Buck, Tunia, Krzysztof, Zhao, Lei, Mylnikova, Liudmila N., Larsson, Lars, Metz, Laure, Yepiskoposyan, Levon, Pedersen, Lisbeth, Sarti, Lucia, Orlando, Ludovic, Slimak, Ludovic, Klassen, Lutz, Blank, Malou, González-Morales, Manuel, Silvestrini, Mara, Vretemark, Maria, Nesterova, Marina S., Rykun, Marina, Rolfo, Mario Federico, Szmyt, Marzena, Przybyła, Marcin, Calattini, Mauro, Sablin, Mikhail, Dobisíková, Miluše, Meldgaard, Morten, Johansen, Morten, Berezina, Natalia, Card, Nick, Saveliev, Nikolai A., Poshekhonova, Olga, Rickards, Olga, Lozovskaya, Olga V., Gábor, Olivér, Uldum, Otto Christian, Aurino, Paola, Kosintsev, Pavel, Courtaud, Patrice, Ríos, Patricia, Mortensen, Peder, Lotz, Per, Persson, Per, Bangsgaard, Pernille, Barros Damgaard, Peter de, Petersen, Peter Vang, Prieto Martínez, Pilar, Włodarczak, Piotr, Smolyaninov, Roman V., Maring, Rikke, Menduiña, Roberto, Badalyan, Ruben, Iversen, Rune, Turin, Ruslan, Vasilyev, Sergey, Wåhlin, Sidsel, Borutskaya, Svetlana, Skochina, Svetlana, Sørensen, Søren Anker, Andersen, Søren H., Jørgensen, Thomas, Serikov, Yuri B., Molodin, Vyacheslav I., Smrcka, Vaclav, Merts, Victor, Appadurai, Vivek, Moiseyev, Vyacheslav, Magnusson, Yvonne, Kjær, Kurt H., Lynnerup, Niels, Lawson, Daniel J., Sudmant, Peter H., Rasmussen, Simon, Korneliussen, Thorfinn Sand, Durbin, Richard, Nielsen, Rasmus, Delaneau, Olivier, Werge, Thomas, Racimo, Fernando, Kristiansen, Kristian, Willerslev, Eske, Lundbeck Foundation, Novo Nordisk Foundation, Wellcome Trust, Carlsberg Foundation, Danish National Research Foundation, University of Copenhagen, Ferring Pharmaceuticals, Swedish Foundation for Humanities and Social Sciences, Villum Fonden, Independent Research Fund Denmark, Hanne and Torkel Weis-Fogh Fund, Wellcome, Swiss National Science Foundation, European Research Council, Aarhus University Research Foundation, Ministry of Education and Science (Kazakhstan), Ministerio de Economía y Competitividad (España), Ministero dell'Istruzione, dell'Università e della Ricerca, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Generalitat Valenciana, Nomis Foundation, European Commission, Ministry of Science and Higher Education of the Russian Federation, Ministry of Education, Science, Culture and Sports of the Republic of Armenia, National Institute of General Medical Sciences (US), Sikora, Martin [0000-0003-2818-8319], Lalueza-Fox, Carles [0000-0002-1730-5914], Allentoft, Morten, Sikora, Martin, Refoyo-Martínez, Alba, Irving-Pease, Evan K., Fischer, Anders, Barrie, William, Ingason, Andrés, Stenderup, Jesper, Sjögren, Karl-Göran, Pearson, Alice, Mota, Bárbara Sousa da, Schulz Paulsson, Bettina, Halgren, Alma, Macleod, Ruairidh, Jørkov, Marie Louise S., Demeter, Fabrice, Sørensen, Lasse, Nielsen, Poul Otto, Henriksen, Rasmus A., Vimala, Tharsika, McColl, Hugh, Margaryan, Ashot, Ilardo, Melissa, Vaughn, Andrew, Fischer Mortensen, Morten, Nielsen, Anne Birgitte, Ulfeldt Hede, Mikkel, Johannsen, Niels Nørkjær, Rasmussen, Peter, Vinner, Lasse, Renaud, Gabriel, Stern, Aaron, Trolle Jensen, Theis Zetner, Scorrano, Gabriele, Schroeder, Hannes, Lysdahl, Per, Ramsøe, Abigail Daisy, Skorobogatov, Andrei, Schork, Andrew Joseph, Rosengren, Anders, Ruter, Anthony, Outram, Alan, Timoshenko, Aleksey A., Buzhilova, Alexandra, Coppa, Alfredo, Zubova, Alisa, Silva, Ana María, Hansen, Anders J., Gromov, Andrey, Logvin, Andrey, Gotfredsen, Anne Birgitte, Nielsen, Bjarne Henning, González-Rabanal, Borja, Lalueza-Fox, Carles, McKenzie, Catriona J., Gaunitz, Charleen, Blasco, Concepción, Liesau, Corina, Martínez-Labarga, Cristina, Pozdnyakov, Dmitri V., Cuenca-Solana, David, Lordkipanidze, David O., En’shin, Dmitri, Salazar García, Domingo Carlos, Price, T. Douglas, Borić, Dušan, Kostyleva, Elena, Veselovskaya, Elizaveta V., Usmanova, Emma R., Cappellini, Enrico, Petersen, Erik Brinch, Kannegaard, Esben, Radina, Francesca, Yediay, Fulya Eylem, Duday, Henri, Gutiérrez-Zugasti, Igor, Merts, Ilya, Potekhina, Inna, Shevnina, Irina, Altinkaya, Isin, Guilaine, Jean, Hansen, Jesper, Aura Tortosa, Joan Emili, Zilhão, João, Vega, Jorge, Pedersen, Kristoffer Buck, Tunia, Krzysztof, Zhao, Lei, Mylnikova, Liudmila N., Larsson, Lars, Metz, Laure, Yepiskoposyan, Levon, Pedersen, Lisbeth, Sarti, Lucia, Orlando, Ludovic, Slimak, Ludovic, Klassen, Lutz, Blank, Malou, González-Morales, Manuel, Silvestrini, Mara, Vretemark, Maria, Nesterova, Marina S., Rykun, Marina, Rolfo, Mario Federico, Szmyt, Marzena, Przybyła, Marcin, Calattini, Mauro, Sablin, Mikhail, Dobisíková, Miluše, Meldgaard, Morten, Johansen, Morten, Berezina, Natalia, Card, Nick, Saveliev, Nikolai A., Poshekhonova, Olga, Rickards, Olga, Lozovskaya, Olga V., Gábor, Olivér, Uldum, Otto Christian, Aurino, Paola, Kosintsev, Pavel, Courtaud, Patrice, Ríos, Patricia, Mortensen, Peder, Lotz, Per, Persson, Per, Bangsgaard, Pernille, Barros Damgaard, Peter de, Petersen, Peter Vang, Prieto Martínez, Pilar, Włodarczak, Piotr, Smolyaninov, Roman V., Maring, Rikke, Menduiña, Roberto, Badalyan, Ruben, Iversen, Rune, Turin, Ruslan, Vasilyev, Sergey, Wåhlin, Sidsel, Borutskaya, Svetlana, Skochina, Svetlana, Sørensen, Søren Anker, Andersen, Søren H., Jørgensen, Thomas, Serikov, Yuri B., Molodin, Vyacheslav I., Smrcka, Vaclav, Merts, Victor, Appadurai, Vivek, Moiseyev, Vyacheslav, Magnusson, Yvonne, Kjær, Kurt H., Lynnerup, Niels, Lawson, Daniel J., Sudmant, Peter H., Rasmussen, Simon, Korneliussen, Thorfinn Sand, Durbin, Richard, Nielsen, Rasmus, Delaneau, Olivier, Werge, Thomas, Racimo, Fernando, Kristiansen, Kristian, and Willerslev, Eske

Abstract

Western Eurasia witnessed several large-scale human migrations during the Holocene1,2,3,4,5. Here, to investigate the cross-continental effects of these migrations, we shotgun-sequenced 317 genomes—mainly from the Mesolithic and Neolithic periods—from across northern and western Eurasia. These were imputed alongside published data to obtain diploid genotypes from more than 1,600 ancient humans. Our analyses revealed a ‘great divide’ genomic boundary extending from the Black Sea to the Baltic. Mesolithic hunter-gatherers were highly genetically differentiated east and west of this zone, and the effect of the neolithization was equally disparate. Large-scale ancestry shifts occurred in the west as farming was introduced, including near-total replacement of hunter-gatherers in many areas, whereas no substantial ancestry shifts happened east of the zone during the same period. Similarly, relatedness decreased in the west from the Neolithic transition onwards, whereas, east of the Urals, relatedness remained high until around 4,000 BP, consistent with the persistence of localized groups of hunter-gatherers. The boundary dissolved when Yamnaya-related ancestry spread across western Eurasia around 5,000 BP, resulting in a second major turnover that reached most parts of Europe within a 1,000-year span. The genetic origin and fate of the Yamnaya have remained elusive, but we show that hunter-gatherers from the Middle Don region contributed ancestry to them. Yamnaya groups later admixed with individuals associated with the Globular Amphora culture before expanding into Europe. Similar turnovers occurred in western Siberia, where we report new genomic data from a ‘Neolithic steppe’ cline spanning the Siberian forest steppe to Lake Baikal. These prehistoric migrations had profound and lasting effects on the genetic diversity of Eurasian populations.

Published
2024

24. Population genomics of post-glacial western Eurasia

Author

Allentoft, Morten, Sikora, Martin, Refoyo-Martínez, Alba, Irving-Pease, Evan, Fischer, Anders, Barrie, William, Ingason, Andrés, Stenderup, Jesper, Sjögren, Karl-Göran, Pearson, Alice, Sousa da Mota, Bárbara, Schulz Paulsson, Bettina, Halgren, Alma, Macleod, Ruairidh, Schjellerup Jørkov, Marie Louise, Demeter, Fabrice, Sørensen, Lasse, Nielsen, Poul Otto, Henriksen, Rasmus, Vimala, Tharsika, McColl, Hugh, Margaryan, Ashot, Ilardo, Melissa, Vaughn, Andrew, Mortensen, Morten Fischer, Nielsen, Anne Birgitte, Ulfeldt Hede, Mikkel, Johannsen, Niels Nørkjær, Rasmussen, Peter, Vinner, Lasse, Renaud, Gabriel, Stern, Aaron, Jensen, Theis, Scorrano, Gabriele, Schroeder, Hannes, Lysdahl, Per, Ramsøe, Abigail Daisy, Skorobogatov, Andrei, Schork, Andrew Joseph, Rosengren, Anders, Ruter, Anthony, Outram, Alan, Timoshenko, Aleksey, Buzhilova, Alexandra, Coppa, Alfredo, Zubova, Alisa, Silva, Ana Maria, Hansen, Anders, Gromov, Andrey, Logvin, Andrey, Gotfredsen, Anne Birgitte, Nielsen, Bjarne Henning, González-Rabanal, Borja, Lalueza-Fox, Carles, McKenzie, Catriona, Gaunitz, Charleen, Blasco, Concepción, Liesau, Corina, Martinez-Labarga, Cristina, Pozdnyakov, Dmitri, Cuenca-Solana, David, Lordkipanidze, David, En’shin, Dmitri, Salazar-García, Domingo, Price, Douglas, Borić, Dušan, Kostyleva, Elena, Veselovskaya, Elizaveta, Usmanova, Emma, Cappellini, Enrico, Petersen, Erik Brinch, Kannegaard, Esben, Radina, Francesca, Yediay, Fulya Eylem, Duday, Henri, Gutiérrez-Zugasti, Igor, Merts, Ilya, Potekhina, Inna, Shevnina, Irina, Altinkaya, Isin, Guilaine, Jean, Hansen, Jesper, Aura Tortosa, Joan Emili, Zilhão, João, Vega, Jorge, Buck Pedersen, Kristoffer, Tunia, Krzysztof, Zhao, Lei, Mylnikova, Liudmila, Larsson, Lars, Metz, Laure, Yepiskoposyan, Levon, Pedersen, Lisbeth, Sarti, Lucia, Orlando, Ludovic, Slimak, Ludovic, Klassen, Lutz, Blank, Malou, González-Morales, Manuel, Silvestrini, Mara, Vretemark, Maria, Nesterova, Marina, Rykun, Marina, Rolfo, Mario Federico, Szmyt, Marzena, Przybyła, Marcin, Calattini, Mauro, Sablin, Mikhail, Dobisíková, Miluše, Meldgaard, Morten, Johansen, Morten, Berezina, Natalia, Card, Nick, Saveliev, Nikolai, Poshekhonova, Olga, Rickards, Olga, Lozovskaya, Olga, Gábor, Olivér, Uldum, Otto Christian, Aurino, Paola, Kosintsev, Pavel, Courtaud, Patrice, Ríos, Patricia, Mortensen, Peder, Lotz, Per, Persson, Per, Bangsgaard, Pernille, Damgaard, Peter de Barros, Petersen, Peter Vang, Prieto Martínez, Pilar, Włodarczak, Piotr, Smolyaninov, Roman, Maring, Rikke, Menduiña, Roberto, Badalyan, Ruben, Iversen, Rune, Turin, Ruslan, Vasilyev, Sergey, Wåhlin, Sidsel, Borutskaya, Svetlana, Skochina, Svetlana, Sørensen, Søren Anker, Andersen, Søren, Jørgensen, Thomas, Serikov, Yuri, Molodin, Vyacheslav, Smrcka, Vaclav, Merts, Victor, Appadurai, Vivek, Moiseyev, Vyacheslav, Magnusson,Yvonne, Kjær, Kurt, Lynnerup, Niels, Lawson, Daniel, Sudmant, Peter, Rasmussen, Simon, Korneliussen, Thorfinn Sand, Durbin, Richard, Nielsen, Rasmus, Delanea, Olivier, Werge, Thomas, Racimo, Fernando, Kristiansen, Kristian, Willerslev, Eske, Allentoft, Morten, Sikora, Martin, Refoyo-Martínez, Alba, Irving-Pease, Evan, Fischer, Anders, Barrie, William, Ingason, Andrés, Stenderup, Jesper, Sjögren, Karl-Göran, Pearson, Alice, Sousa da Mota, Bárbara, Schulz Paulsson, Bettina, Halgren, Alma, Macleod, Ruairidh, Schjellerup Jørkov, Marie Louise, Demeter, Fabrice, Sørensen, Lasse, Nielsen, Poul Otto, Henriksen, Rasmus, Vimala, Tharsika, McColl, Hugh, Margaryan, Ashot, Ilardo, Melissa, Vaughn, Andrew, Mortensen, Morten Fischer, Nielsen, Anne Birgitte, Ulfeldt Hede, Mikkel, Johannsen, Niels Nørkjær, Rasmussen, Peter, Vinner, Lasse, Renaud, Gabriel, Stern, Aaron, Jensen, Theis, Scorrano, Gabriele, Schroeder, Hannes, Lysdahl, Per, Ramsøe, Abigail Daisy, Skorobogatov, Andrei, Schork, Andrew Joseph, Rosengren, Anders, Ruter, Anthony, Outram, Alan, Timoshenko, Aleksey, Buzhilova, Alexandra, Coppa, Alfredo, Zubova, Alisa, Silva, Ana Maria, Hansen, Anders, Gromov, Andrey, Logvin, Andrey, Gotfredsen, Anne Birgitte, Nielsen, Bjarne Henning, González-Rabanal, Borja, Lalueza-Fox, Carles, McKenzie, Catriona, Gaunitz, Charleen, Blasco, Concepción, Liesau, Corina, Martinez-Labarga, Cristina, Pozdnyakov, Dmitri, Cuenca-Solana, David, Lordkipanidze, David, En’shin, Dmitri, Salazar-García, Domingo, Price, Douglas, Borić, Dušan, Kostyleva, Elena, Veselovskaya, Elizaveta, Usmanova, Emma, Cappellini, Enrico, Petersen, Erik Brinch, Kannegaard, Esben, Radina, Francesca, Yediay, Fulya Eylem, Duday, Henri, Gutiérrez-Zugasti, Igor, Merts, Ilya, Potekhina, Inna, Shevnina, Irina, Altinkaya, Isin, Guilaine, Jean, Hansen, Jesper, Aura Tortosa, Joan Emili, Zilhão, João, Vega, Jorge, Buck Pedersen, Kristoffer, Tunia, Krzysztof, Zhao, Lei, Mylnikova, Liudmila, Larsson, Lars, Metz, Laure, Yepiskoposyan, Levon, Pedersen, Lisbeth, Sarti, Lucia, Orlando, Ludovic, Slimak, Ludovic, Klassen, Lutz, Blank, Malou, González-Morales, Manuel, Silvestrini, Mara, Vretemark, Maria, Nesterova, Marina, Rykun, Marina, Rolfo, Mario Federico, Szmyt, Marzena, Przybyła, Marcin, Calattini, Mauro, Sablin, Mikhail, Dobisíková, Miluše, Meldgaard, Morten, Johansen, Morten, Berezina, Natalia, Card, Nick, Saveliev, Nikolai, Poshekhonova, Olga, Rickards, Olga, Lozovskaya, Olga, Gábor, Olivér, Uldum, Otto Christian, Aurino, Paola, Kosintsev, Pavel, Courtaud, Patrice, Ríos, Patricia, Mortensen, Peder, Lotz, Per, Persson, Per, Bangsgaard, Pernille, Damgaard, Peter de Barros, Petersen, Peter Vang, Prieto Martínez, Pilar, Włodarczak, Piotr, Smolyaninov, Roman, Maring, Rikke, Menduiña, Roberto, Badalyan, Ruben, Iversen, Rune, Turin, Ruslan, Vasilyev, Sergey, Wåhlin, Sidsel, Borutskaya, Svetlana, Skochina, Svetlana, Sørensen, Søren Anker, Andersen, Søren, Jørgensen, Thomas, Serikov, Yuri, Molodin, Vyacheslav, Smrcka, Vaclav, Merts, Victor, Appadurai, Vivek, Moiseyev, Vyacheslav, Magnusson,Yvonne, Kjær, Kurt, Lynnerup, Niels, Lawson, Daniel, Sudmant, Peter, Rasmussen, Simon, Korneliussen, Thorfinn Sand, Durbin, Richard, Nielsen, Rasmus, Delanea, Olivier, Werge, Thomas, Racimo, Fernando, Kristiansen, Kristian, and Willerslev, Eske

Abstract

Western Eurasia witnessed several large-scale human migrations during the Holocene1–5. Here, to investigate the cross-continental effects of these migrations, we shotgun-sequenced 317 genomes—mainly from the Mesolithic and Neolithic periods— from across northern and western Eurasia. These were imputed alongside published data to obtain diploid genotypes from more than 1,600 ancient humans. Our analyses revealed a ‘great divide’ genomic boundary extending from the Black Sea to the Baltic. Mesolithic hunter-gatherers were highly genetically differentiated east and west of this zone, and the effect of the neolithization was equally disparate. Large-scale ancestry shifts occurred in the west as farming was introduced, including near-total replacement of hunter-gatherers in many areas, whereas no substantial ancestry shifts happened east of the zone during the same period. Similarly, relatedness decreased in the west from the Neolithic transition onwards, whereas, east of the Urals, relatedness remained high until around 4,000 bp, consistent with the persistence of localized groups of hunter-gatherers. The boundary dissolved when Yamnaya-related ancestry spread across western Eurasia around 5,000 bp, resulting in a second major turnover that reached most parts of Europe within a 1,000-year span. The genetic origin and fate of the Yamnaya have remained elusive, but we show that hunter-gatherers from the Middle Don region contributed ancestry to them. Yamnaya groups later admixed with individuals associated with the Globular Amphora culture before expanding into Europe. Similar turnovers occurred in western Siberia, where we report new genomic data from a ‘Neolithic steppe’ cline spanning the Siberian forest steppe to Lake Baikal. These prehistoric migrations had profound and lasting effects on the genetic diversity of Eurasian populations.

Published
2024

25. Groundwater is a hidden global keystone ecosystem

Author

Saccò, Mattia, Mammola, Stefano, Altermatt, Florian, Alther, Roman, Bolpagni, Rossano, Brancelj, Anton, Brankovits, David, Fišer, Cene, Gerovasileiou, Vasilis, Griebler, Christian, Guareschi, Simone, Hose, Grant C., Korbel, Kathryn, Lictevout, Elisabeth, Malard, Florian, Martínez, Alejandro, Niemiller, Matthew L., Robertson, Anne, Tanalgo, Krizler C., Bichuette, Maria Elina, Borko, Špela, Brad, Traian, Campbell, Matthew A., Cardoso, Pedro, Celico, Fulvio, Cooper, Steven J. B., Culver, David, Di Lorenzo, Tiziana, Galassi, Diana M. P., Guzik, Michelle T., Hartland, Adam, Humphreys, William F., Ferreira, Rodrigo Lopes, Lunghi, Enrico, Nizzoli, Daniele, Perina, Giulia, Raghavan, Rajeev, Richards, Zoe, Reboleira, Ana Sofia P. S., Rohde, Melissa M., Fernández, David Sánchez, Schmidt, Susanne I., van der Heyde, Mieke, Weaver, Louise, White, Nicole E., Zagmajster, Maja, Hogg, Ian, Ruhi, Albert, Gagnon, Marthe M., Allentoft, Morten E., Reinecke, Robert, Saccò, Mattia, Mammola, Stefano, Altermatt, Florian, Alther, Roman, Bolpagni, Rossano, Brancelj, Anton, Brankovits, David, Fišer, Cene, Gerovasileiou, Vasilis, Griebler, Christian, Guareschi, Simone, Hose, Grant C., Korbel, Kathryn, Lictevout, Elisabeth, Malard, Florian, Martínez, Alejandro, Niemiller, Matthew L., Robertson, Anne, Tanalgo, Krizler C., Bichuette, Maria Elina, Borko, Špela, Brad, Traian, Campbell, Matthew A., Cardoso, Pedro, Celico, Fulvio, Cooper, Steven J. B., Culver, David, Di Lorenzo, Tiziana, Galassi, Diana M. P., Guzik, Michelle T., Hartland, Adam, Humphreys, William F., Ferreira, Rodrigo Lopes, Lunghi, Enrico, Nizzoli, Daniele, Perina, Giulia, Raghavan, Rajeev, Richards, Zoe, Reboleira, Ana Sofia P. S., Rohde, Melissa M., Fernández, David Sánchez, Schmidt, Susanne I., van der Heyde, Mieke, Weaver, Louise, White, Nicole E., Zagmajster, Maja, Hogg, Ian, Ruhi, Albert, Gagnon, Marthe M., Allentoft, Morten E., and Reinecke, Robert

Abstract

Groundwater is a vital ecosystem of the global water cycle, hosting unique biodiversity and providing essential services to societies. Despite being the largest unfrozen freshwater resource, in a period of depletion by extraction and pollution, groundwater environments have been repeatedly overlooked in global biodiversity conservation agendas. Disregarding the importance of groundwater as an ecosystem ignores its critical role in preserving surface biomes. To foster timely global conservation of groundwater, we propose elevating the concept of keystone species into the realm of ecosystems, claiming groundwater as a keystone ecosystem that influences the integrity of many dependent ecosystems. Our global analysis shows that over half of land surface areas (52.6%) has a medium-to-high interaction with groundwater, reaching up to 74.9% when deserts and high mountains are excluded. We postulate that the intrinsic transboundary features of groundwater are critical for shifting perspectives towards more holistic approaches in aquatic ecology and beyond. Furthermore, we propose eight key themes to develop a science-policy integrated groundwater conservation agenda. Given ecosystems above and below the ground intersect at many levels, considering groundwater as an essential component of planetary health is pivotal to reduce biodiversity loss and buffer against climate change.

Published
2024

26. Vittrup Man–The life-history of a genetic foreigner in Neolithic Denmark

Author

Fischer, Anders, Sjögren, Karl-Göran, Jensen, Theis Zetner Trolle, Jørkov, Marie Louise, Lysdahl, Per, Vimala, Tharsika, Refoyo-martínez, Alba, Scorrano, Gabriele, Price, T. Douglas, Gröcke, Darren R., Gotfredsen, Anne Birgitte, Sørensen, Lasse, Alexandersen, Verner, Wåhlin, Sidsel, Stenderup, Jesper, Bennike, Ole, Ingason, Andrés, Iversen, Rune, Sikora, Martin, Racimo, Fernando, Willerslev, Eske, Allentoft, Morten E., Kristiansen, Kristian, Fischer, Anders, Sjögren, Karl-Göran, Jensen, Theis Zetner Trolle, Jørkov, Marie Louise, Lysdahl, Per, Vimala, Tharsika, Refoyo-martínez, Alba, Scorrano, Gabriele, Price, T. Douglas, Gröcke, Darren R., Gotfredsen, Anne Birgitte, Sørensen, Lasse, Alexandersen, Verner, Wåhlin, Sidsel, Stenderup, Jesper, Bennike, Ole, Ingason, Andrés, Iversen, Rune, Sikora, Martin, Racimo, Fernando, Willerslev, Eske, Allentoft, Morten E., and Kristiansen, Kristian

Abstract

The lethally maltreated body of Vittrup Man was deposited in a Danish bog, probably as part of a ritualised sacrifice. It happened between c. 3300 and 3100 cal years BC, i.e., during the period of the local farming-based Funnel Beaker Culture. In terms of skull morphological features, he differs from the majority of the contemporaneous farmers found in Denmark, and associates with hunter-gatherers, who inhabited Scandinavia during the previous millennia. His skeletal remains were selected for transdisciplinary analysis to reveal his life-history in terms of a population historical perspective. We report the combined results of an integrated set of genetic, isotopic, physical anthropological and archaeological analytical approaches. Strontium signature suggests a foreign birthplace that could be in Norway or Sweden. In addition, enamel oxygen isotope values indicate that as a child he lived in a colder climate, i.e., to the north of the regions inhabited by farmers. Genomic data in fact demonstrates that he is closely related to Mesolithic humans known from Norway and Sweden. Moreover, dietary stable isotope analyses on enamel and bone collagen demonstrate a fisher-hunter way of life in his childhood and a diet typical of farmers later on. Such a variable life-history is also reflected by proteomic analysis of hardened organic deposits on his teeth, indicating the consumption of forager food (seal, whale and marine fish) as well as farmer food (sheep/goat). From a dietary isotopic transect of one of his teeth it is shown that his transfer between societies of foragers and farmers took place near to the end of his teenage years.

Published
2024

27. Spider webs capture environmental DNA from terrestrial vertebrates

Author

Newton, Joshua, Nevill, Paul, Bateman, Phillip, Campbell, Mathew, Allentoft, Morten, Newton, Joshua, Nevill, Paul, Bateman, Phillip, Campbell, Mathew, and Allentoft, Morten

Abstract

Environmental DNA holds significant promise as a non-invasive tool for tracking terrestrial biodiversity. However, in non-homogenous terrestrial environments, the continual exploration of new substrates is crucial. Here we test the hypothesis that spider webs can act as passive biofilters, capturing eDNA from vertebrates present in the local environment. Using a metabarcoding approach, we detected verte brate eDNA from all analyzed spider webs (N = 49). Spider webs obtained from an Australian woodland locality yielded vertebrate eDNA from 32 different species, including native mammals and birds. In contrast, webs from Perth Zoo, less than 50 km away, yielded eDNA from 61 different vertebrates and produced a highly distinct species composition, largely reflecting exotic species hosted in the zoo. We show that higher animal biomass and proximity to animal enclosures increased eDNA detection probabil ity in the zoo. Our results indicate a tremendous potential for using spider webs as a cost-effective means to monitor terrestrial vertebrates.

Published
2024

28. Far away from home? Ancient DNA shows the presence of bicolored shrew (Crocidura leucodon) in Bronze Age Denmark.

Author

Mousavi‐Derazmahalleh, Mahsa, Haue, Niels, Kanstrup, Marie, Laursen, Jørgen T., Lukehurst, Sherralee S., Kveiborg, Jacob, and Allentoft, Morten E.

Subjects
MITOCHONDRIAL DNA, FOSSIL DNA, BRONZE Age, IRON Age, NATURAL history
Abstract

An excavation of an Early Iron Age village near Aalborg in Denmark uncovered the jaws and skull fragments from a small mammal that were morphologically identified to the genus Crocidura (white‐toothed shrews). Three Crocidura species are known from prehistoric continental Europe but none of them are distributed in Scandinavia, which is why this surprising finding warranted further analyses. The bone was radiocarbon‐dated to 2840–2750 calibrated years before present (cal. BP), corresponding to the Late Bronze Age and hence earlier than the Iron Age archeological context in which it was found. Using highly optimized ancient DNA protocols, we extracted DNA from one tooth and shotgun‐sequenced the sample to reconstruct a near‐complete mitochondrial reference genome (17,317 bp, 32.6× coverage). Phylogenetic analyses determined this specimen as a bicolored shrew (Crocidura leucodon) but with a phylogenetic position basal to the clade of known sequences from this species. The confirmation of Crocidura presence in Denmark by the Late Bronze Age sheds new light on the prehistoric natural history of Scandinavia. We discuss the implications of this finding from both zoo‐archeological and ecological perspectives. Furthermore, the mitochondrial genome reconstructed in this study offers a valuable resource for future research exploring the genetic makeup and evolutionary history of Eurasian shrew populations. [ABSTRACT FROM AUTHOR]

Published
2024
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30. Vittrup Man–The life-history of a genetic foreigner in Neolithic Denmark

Author

Fischer, Anders, primary, Sjögren, Karl-Göran, additional, Jensen, Theis Zetner Trolle, additional, Jørkov, Marie Louise, additional, Lysdahl, Per, additional, Vimala, Tharsika, additional, Refoyo-Martínez, Alba, additional, Scorrano, Gabriele, additional, Price, T. Douglas, additional, Gröcke, Darren R., additional, Gotfredsen, Anne Birgitte, additional, Sørensen, Lasse, additional, Alexandersen, Verner, additional, Wåhlin, Sidsel, additional, Stenderup, Jesper, additional, Bennike, Ole, additional, Ingason, Andrés, additional, Iversen, Rune, additional, Sikora, Martin, additional, Racimo, Fernando, additional, Willerslev, Eske, additional, Allentoft, Morten E., additional, and Kristiansen, Kristian, additional

Published
2024
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31. Plastic and genomic change of a newly established lizard population following a founder event

Author

Sabolić, Iva, primary, Mira, Óscar, additional, Brandt, Débora Y. C., additional, Lisičić, Duje, additional, Stapley, Jessica, additional, Novosolov, Maria, additional, Bakarić, Robert, additional, Cizelj, Ivan, additional, Glogoški, Marko, additional, Hudina, Tomislav, additional, Taverne, Maxime, additional, Allentoft, Morten E., additional, Nielsen, Rasmus, additional, Herrel, Anthony, additional, and Štambuk, Anamaria, additional

Published
2023
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32. Groundwater is a hidden global keystone ecosystem

Author

Saccò, Mattia, primary, Mammola, Stefano, additional, Altermatt, Florian, additional, Alther, Roman, additional, Bolpagni, Rossano, additional, Brancelj, Anton, additional, Brankovits, David, additional, Fišer, Cene, additional, Gerovasileiou, Vasilis, additional, Griebler, Christian, additional, Guareschi, Simone, additional, Hose, Grant C., additional, Korbel, Kathryn, additional, Lictevout, Elisabeth, additional, Malard, Florian, additional, Martínez, Alejandro, additional, Niemiller, Matthew L., additional, Robertson, Anne, additional, Tanalgo, Krizler C., additional, Bichuette, Maria Elina, additional, Borko, Špela, additional, Brad, Traian, additional, Campbell, Matthew A., additional, Cardoso, Pedro, additional, Celico, Fulvio, additional, Cooper, Steven J. B., additional, Culver, David, additional, Di Lorenzo, Tiziana, additional, Galassi, Diana M. P., additional, Guzik, Michelle T., additional, Hartland, Adam, additional, Humphreys, William F., additional, Ferreira, Rodrigo Lopes, additional, Lunghi, Enrico, additional, Nizzoli, Daniele, additional, Perina, Giulia, additional, Raghavan, Rajeev, additional, Richards, Zoe, additional, Reboleira, Ana Sofia P. S., additional, Rohde, Melissa M., additional, Fernández, David Sánchez, additional, Schmidt, Susanne I., additional, van der Heyde, Mieke, additional, Weaver, Louise, additional, White, Nicole E., additional, Zagmajster, Maja, additional, Hogg, Ian, additional, Ruhi, Albert, additional, Gagnon, Marthe M., additional, Allentoft, Morten E., additional, and Reinecke, Robert, additional

Published
2023
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33. Plastic and genomic change of a newly established lizard population following a founder event.

Author

Sabolić, Iva, Mira, Óscar, Brandt, Débora Y. C., Lisičić, Duje, Stapley, Jessica, Novosolov, Maria, Bakarić, Robert, Cizelj, Ivan, Glogoški, Marko, Hudina, Tomislav, Taverne, Maxime, Allentoft, Morten E., Nielsen, Rasmus, Herrel, Anthony, and Štambuk, Anamaria

Subjects
LIZARD populations, GENOTYPE-environment interaction, HERITABILITY, PHENOTYPIC plasticity, POPULATION differentiation, LACERTIDAE, PHENOTYPES
Abstract

Understanding how phenotypic divergence arises among natural populations remains one of the major goals in evolutionary biology. As part of competitive exclusion experiment conducted in 1971, 10 individuals of Italian wall lizard (Podarcis siculus (Rafinesque‐Schmaltz, 1810)) were transplanted from Pod Kopište Island to the nearby island of Pod Mrčaru (Adriatic Sea). Merely 35 years after the introduction, the newly established population on Pod Mrčaru Island had shifted their diet from predominantly insectivorous towards omnivorous and changed significantly in a range of morphological, behavioural, physiological and ecological characteristics. Here, we combine genomic and quantitative genetic approaches to determine the relative roles of genetic adaptation and phenotypic plasticity in driving this rapid phenotypic shift. Our results show genome‐wide genetic differentiation between ancestral and transplanted population, with weak genetic erosion on Pod Mrčaru Island. Adaptive processes following the founder event are indicated by highly differentiated genomic loci associating with ecologically relevant phenotypic traits, and/or having a putatively adaptive role across multiple lizard populations. Diverged traits related to head size and shape or bite force showed moderate heritability in a crossing experiment, but between‐population differences in these traits did not persist in a common garden environment. Our results confirm the existence of sufficient additive genetic variance for traits to evolve under selection while also demonstrating that phenotypic plasticity and/or genotype by environment interactions are the main drivers of population differentiation at this early evolutionary stage. [ABSTRACT FROM AUTHOR]

Published
2024
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34. The landscape of ancient human pathogens in Eurasia from the Stone Age to historical times

Author

Sikora, Martin, primary, Canteri, Elisabetta, additional, Fernandez-Guerra, Antonio, additional, Oskolkov, Nikolay, additional, Agren, Rasmus, additional, Hansson, Lena, additional, Irving-Pease, Evan K, additional, Muehlemann, Barbara, additional, Holtsmark Nielsen, Sofie, additional, Scorrano, Gabriele, additional, Allentoft, Morten E, additional, Seersholm, Frederik Valeur, additional, Schroeder, Hannes, additional, Gaunitz, Charleen, additional, Stenderup, Jesper, additional, Vinner, Lasse, additional, Jones, Terry C, additional, Nystedt, Bjorn, additional, Parkhill, Julian, additional, Fugger, Lars, additional, Racimo, Fernando, additional, Kristiansen, Kristian, additional, Iversen, Astrid K N, additional, and Willerslev, Eske, additional

Published
2023
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35. Threatened North African seagrass meadows have supported green turtle populations for millennia

Author

de Kock, Willemien, primary, Mackie, Meaghan, additional, Ramsøe, Max, additional, Allentoft, Morten E., additional, Broderick, Annette C., additional, Haywood, Julia C., additional, Godley, Brendan J., additional, Snape, Robin T. E., additional, Bradshaw, Phil J., additional, Genz, Hermann, additional, von Tersch, Matthew, additional, Dee, Michael W., additional, Palsbøll, Per J., additional, Alexander, Michelle, additional, Taurozzi, Alberto J., additional, and Çakırlar, Canan, additional

Published
2023
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36. 42 imputed downsampled ancient human genomes

Author

Sousa da Mota, Bárbara, Rubinacci, Simone, Cruz Dávalos, Diana I., G. Amorim, Carlos Eduardo, Sikora, Martin, Johannsen, Niels N., Szmyt, Marzena H., Włodarcza, Piotr, Szczepanek, Anita, Przybyła, Marcin M., Schroeder, Hannes, Allentoft, Morten E., Willerslev, Eske, Malaspinas, Anna-Sapfo, and Delaneau, Olivier

Subjects
aDNA, genomics, imputation
Abstract

This is the dataset of imputed downsampled ancient genomes that we used to assess imputation accuracy of ancient human genomes (for more information, please read our manuscript "Imputation of ancient human genomes"). We downsampled 42 high-coverage ancient genomes (>10x) to coverages 0.1x, 0.25x, 0.5x, 0.75x, 1.0x and 2.0x and we imputed the downsampled data with GLIMPSE v1.1.1 (Rubinacci et al., Nature Genetics (2021)) and using 1000 Genomes as a reference panel., {"references":["Valdiosera, C. et al. Four millennia of Iberian biomolecular prehistory illustrate the impact of prehistoric migrations at the far end of Eurasia. Proc. Natl. Acad. Sci. U. S. A. 115, 3428–3433 (2018)","Lazaridis, I. et al. Ancient human genomes suggest three ancestral populations for present-day Europeans. Nature 513, 409–413 (2014)","Cassidy, L. M. et al. Neolithic and Bronze Age migration to Ireland and establishment of the insular atlantic genome. Proc. Natl. Acad. Sci. U. S. A. 113, 368–373 (2016)","Günther, T. et al. Population genomics of Mesolithic Scandinavia : Investigating early postglacial migration routes and high-latitude adaptation. PLoS Biol. 16, e2003703 (2018)","Gamba, C. et al. Genome flux and stasis in a five millennium transect of European prehistory. Nat. Commun. 5, 1–9 (2014)","Sikora, M. et al. Ancient genomes show social and reproductive behavior of early Upper Paleolithic foragers. Science 358, 659–662 (2017)","Ebenesersdóttir, S. S. et al. Ancient genomes from Iceland reveal the making of a human population. Science 360, 1028–1032 (2018)","Margaryan, A. et al. Population genomics of the Viking world. Nature 585, 390–396 (2020)","Amorim, C. E. G. et al. Understanding 6th-century barbarian social organization and migration through paleogenomics. Nat. Commun. 9, 3547 (2018)","Schlebusch, C. M. et al. Southern African ancient genomes estimate modern human divergence to 350,000 to 260,000 years ago. Science 358, 652–655 (2017)","Lipson, M. et al. Ancient West African foragers in the context of African population history. Nature 577, 665–670 (2020)","Gallego Llorente, M. et al. Ancient Ethiopian genome reveals extensive Eurasian admixture throughout the African continent. Science 350, 820–822 (2015)","Jones, E. R. et al. Upper Palaeolithic genomes reveal deep roots of modern Eurasians. Nat. Commun. 6, 1–8 (2015)","Broushaki, F. et al. Early Neolithic genomes from the eastern Fertile Crescent. Science 353, 499–503 (2016)","de Barros Damgaard, P. et al. The first horse herders and the impact of early Bronze Age steppe expansions into Asia. Science 360, (2018)","Moreno-Mayar, J. V. et al. Early human dispersals within the Americas. Science 362, (2018)","Fu, Q. et al. Genome sequence of a 45,000-year-old modern human from western Siberia. Nature 514, 445–449 (2014)","Sikora, M. et al. The population history of northeastern Siberia since the Pleistocene. Nature 570, 182–188 (2019)","Rasmussen, M. et al. Ancient human genome sequence of an extinct Palaeo-Eskimo. Nature 463, 757–762 (2010)","Moreno-Mayar, J. V. et al. Terminal Pleistocene Alaskan genome reveals first founding population of Native Americans. Nature 553, 203–207 (2018)","Schroeder, H. et al. Unraveling ancestry, kinship, and violence in a Late Neolithic mass grave. Proc. Natl. Acad. Sci. U. S. A. 166, 10705–10710 (2019)","Allentoft, M. E. et al. Population Genomics of Stone Age Eurasia. bioRxiv 36, 2022.05.04.490594 (2022)"]}

Published
2023
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37. Aquatic environmental DNA:A review of the macro-organismal biomonitoring revolution

Author

Takahashi, Miwa, Saccò, Mattia, Kestel, Joshua H., Nester, Georgia, Campbell, Matthew A., van der Heyde, Mieke, Heydenrych, Matthew J., Juszkiewicz, David J., Nevill, Paul, Dawkins, Kathryn L., Bessey, Cindy, Fernandes, Kristen, Miller, Haylea, Power, Matthew, Mousavi-Derazmahalleh, Mahsa, Newton, Joshua P., White, Nicole E., Richards, Zoe T., Allentoft, Morten E., Takahashi, Miwa, Saccò, Mattia, Kestel, Joshua H., Nester, Georgia, Campbell, Matthew A., van der Heyde, Mieke, Heydenrych, Matthew J., Juszkiewicz, David J., Nevill, Paul, Dawkins, Kathryn L., Bessey, Cindy, Fernandes, Kristen, Miller, Haylea, Power, Matthew, Mousavi-Derazmahalleh, Mahsa, Newton, Joshua P., White, Nicole E., Richards, Zoe T., and Allentoft, Morten E.

Abstract

Environmental DNA (eDNA) is the fastest growing biomonitoring tool fuelled by two key features: time efficiency and sensitivity. Technological advancements allow rapid biodiversity detection at both species and community levels with increasing accuracy. Concurrently, there has been a global demand to standardise eDNA methods, but this is only possible with an in-depth overview of the technological advancements and a discussion of the pros and cons of available methods. We therefore conducted a systematic literature review of 407 peer-reviewed papers on aquatic eDNA published between 2012 and 2021. We observed a gradual increase in the annual number of publications from four (2012) to 28 (2018), followed by a rapid growth to 124 publications in 2021. This was mirrored by a tremendous diversification of methods in all aspects of the eDNA workflow. For example, in 2012 only freezing was applied to preserve filter samples, whereas we recorded 12 different preservation methods in the 2021 literature. Despite an ongoing standardisation debate in the eDNA community, the field is seemingly moving fast in the opposite direction and we discuss the reasons and implications. Moreover, by compiling the largest PCR-primer database to date, we provide information on 522 and 141 published species-specific and metabarcoding primers targeting a wide range of aquatic organisms. This works as a user-friendly ‘distillation’ of primer information that was hitherto scattered across hundreds of papers, but the list also reflects which taxa are commonly studied with eDNA technology in aquatic environments such as fish and amphibians, and reveals that groups such as corals, plankton and algae are under-studied. Efforts to improve sampling and extraction methods, primer specificity and reference databases are crucial to capture these ecologically important taxa in future eDNA biomonitoring surveys. In a rapidly diversifying field, this review synthetises aquatic eDNA procedures and can gui

Published
2023

38. When nets meet environmental DNA metabarcoding:integrative approach to unveil invertebrate community patterns of hypersaline lakes

Author

Campbell, Matthew A., Laini, Alex, White, Nicole E., Allentoft, Morten E., Saccò, Mattia, Campbell, Matthew A., Laini, Alex, White, Nicole E., Allentoft, Morten E., and Saccò, Mattia

Abstract

Saline and hypersaline wetlands account for almost half of the volume of inland water globally. They provide pivotal habitat for a vast range of species, including crucial ecosystem services for humans such as carbon sink storage and extractive resource reservoirs. Despite their importance, effective ecological assessment is in its infancy compared to current conventional surveys carried out in freshwater ecosystems. The integration of environmental DNA (eDNA) analysis and traditional techniques has the potential to transform biomonitoring processes, particularly in remote and understudied saline environments. In this context, this preliminary study aims to explore the potential of eDNA coupled with conventional approaches by targeting five hypersaline lakes at Rottnest Island (Wadjemup) in Western Australia. We focused on the invertebrate community, a widely accepted key ecological indicator to assess the conservational status in rivers and lakes. The combination of metabarcoding with morphology-based taxonomic analysis described 16 taxa belonging to the orders Anostraca, Diptera, Isopoda, and Coleoptera. DNA-based diversity assessment revealed more taxa at higher taxonomic resolution than the morphology-based taxonomic analysis. However, certain taxa (i.e., Ephydridae, Stratyiomidae, Ceratopogonidae) were only identified via net surveying. Overall, our results indicate that great potential resides in combining conventional net-based surveys with novel eDNA approaches in saline and hypersaline lakes. Indeed, urgent and effective conservational frameworks are required to contrast the enormous pressure that these ecosystems are increasingly facing. Further investigations at larger spatial-temporal scales will allow consolidation of robust, reliable, and affordable biomonitoring frameworks in the underexplored world of saline wetlands.

Published
2023

39. Threatened North African seagrass meadows have supported green turtle populations for millennia

Author

de Kock, Willemien, Mackie, Meaghan, Ramsøe, Max, Allentoft, Morten E., Broderick, Annette C., Haywood, Julia C., Godley, Brendan J., Snape, Robin T.E., Bradshaw, Phil J., Genz, Hermann, von Tersch, Matthew, Dee, Michael W., Palsbøll, Per J., Alexander, Michelle, Taurozzi, Alberto J., Çakırlar, Canan, de Kock, Willemien, Mackie, Meaghan, Ramsøe, Max, Allentoft, Morten E., Broderick, Annette C., Haywood, Julia C., Godley, Brendan J., Snape, Robin T.E., Bradshaw, Phil J., Genz, Hermann, von Tersch, Matthew, Dee, Michael W., Palsbøll, Per J., Alexander, Michelle, Taurozzi, Alberto J., and Çakırlar, Canan

Abstract

"Protect and restore ecosystems and biodiversity" is the second official aim of the current UN Ocean Decade (2021 to 2030) calling for the identification and protection of critical marine habitats. However, data to inform policy are often lacking altogether or confined to recent times, preventing the establishment of long-term baselines. The unique insights gained from combining bioarchaeology (palaeoproteomics, stable isotope analysis) with contemporary data (from satellite tracking) identified habitats which sea turtles have been using in the Eastern Mediterranean over five millennia. Specifically, our analysis of archaeological green turtle (Chelonia mydas) bones revealed that they likely foraged on the same North African seagrass meadows as their modern-day counterparts. Here, millennia-long foraging habitat fidelity has been directly demonstrated, highlighting the significance (and long-term dividends) of protecting these critical coastal habitats that are especially vulnerable to global warming. We highlight the potential for historical ecology to inform policy in safeguarding critical marine habitats.

Published
2023

41. A 2-million-year-old ecosystem in Greenland uncovered by environmental DNA

Author

Kjær, Kurt H, Winther Pedersen, Mikkel, De Sanctis, Bianca, De Cahsan, Binia, Korneliussen, Thorfinn S, Michelsen, Christian S, Sand, Karina K, Jelavić, Stanislav, Ruter, Anthony H, Schmidt, Astrid MA, Kjeldsen, Kristian K, Tesakov, Alexey S, Snowball, Ian, Gosse, John C, Alsos, Inger G, Wang, Yucheng, Dockter, Christoph, Rasmussen, Magnus, Jørgensen, Morten E, Skadhauge, Birgitte, Prohaska, Ana, Kristensen, Jeppe Å, Bjerager, Morten, Allentoft, Morten E, Coissac, Eric, PhyloNorway Consortium, Rouillard, Alexandra, Simakova, Alexandra, Fernandez-Guerra, Antonio, Bowler, Chris, Macias-Fauria, Marc, Vinner, Lasse, Welch, John J, Hidy, Alan J, Sikora, Martin, Collins, Matthew J, Durbin, Richard, Larsen, Nicolaj K, Willerslev, Eske, Kjær, Kurt H [0000-0002-8871-5179], Winther Pedersen, Mikkel [0000-0002-7291-8887], De Sanctis, Bianca [0000-0002-0648-4224], De Cahsan, Binia [0000-0002-6978-6633], Sand, Karina K [0000-0002-0720-7229], Jelavić, Stanislav [0000-0001-7854-3724], Kjeldsen, Kristian K [0000-0002-8557-5131], Gosse, John C [0000-0002-0814-8574], Alsos, Inger G [0000-0002-8610-1085], Wang, Yucheng [0000-0002-7838-226X], Dockter, Christoph [0000-0001-5923-3667], Rasmussen, Magnus [0000-0001-6720-8832], Jørgensen, Morten E [0000-0001-6503-0495], Skadhauge, Birgitte [0000-0001-7317-4376], Prohaska, Ana [0000-0001-5459-6186], Kristensen, Jeppe Å [0000-0001-7168-9405], Coissac, Eric [0000-0001-7507-6729], Rouillard, Alexandra [0000-0001-5778-6620], Fernandez-Guerra, Antonio [0000-0002-8679-490X], Bowler, Chris [0000-0003-3835-6187], Macias-Fauria, Marc [0000-0002-8438-2223], Hidy, Alan J [0000-0003-2916-1425], Sikora, Martin [0000-0003-2818-8319], Durbin, Richard [0000-0002-9130-1006], Willerslev, Eske [0000-0002-7081-6748], and Apollo - University of Cambridge Repository

Subjects
704/158/2464, 631/158/857, Ecology, Fossils, FOS: Biological sciences, Greenland, 704/158/857, article, 45/23, DNA, Environmental, 631/158/2452, Ecosystem
Abstract

Acknowledgements: Acknowledgements: We acknowledge support from the Carlsberg Foundation for logistics to carry out two expeditions to Kap København in 2006 and 2012 (S. Funder, principal investigator for Carlsberg foundation grant to LongTerm and Kap København—the age). The fieldwork in 2016 was supported by a grant to N.K.L. from the Villum Foundation. We highly appreciate the collaborative support by Illumina Inc. that was crucial for the success of the project. E.W. and K.H.K. thank the Danish National Research Foundation (DNRF) and the Lundbeck Foundation (R302-2018-2155) for providing long-term funds to develop the necessary DNA technology that eventually made it possible to retrieve environmental DNA from these ancient deposits in the Kap København Formation. E.W. also acknowledges the Wellcome Trust (UNS69906), the Carlsberg Foundation (CF18-0024), Novo Foundation (NNF18SA0035006), Leverhume (RPG-2016-235) and GRF EXC CRS Chair - Cluster of Excellence (44113220) for their support. M.W.P. acknowledges support from the Carlsberg Foundation (CF17-0275). K.K.S. and S.J. acknowledge support from VILLUM FONDEN (00025352). I.G.A. and E.C. have received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement no. 819192). B.D.S. acknowledges support from the Wellcome Trust programme in Mathematical Genomics and Medicine (WT220023). J.Å.K. was supported by the Carlsberg Foundation (CF20-0238). C.B. acknowledges ERC Advanced Award Diatomic (grant agreement no. 835067). J.C.G. was supported by Natural Science and Engineering Research Council of Canada–Discovery Grant 06785 and Canada Foundation for Innovation grant 21305. M.J.C. acknowledges support from the Danish National Research Foundation DNRF128. We thank G. Yang for cosmogenic isotope AMS target chemistry; S. Funder for introducing us to the Kap København Formation and generating much of the platform that enabled us to conduct our research; T. O. Delmont for providing data and guidance on the SMAGs analysis; Minik Rosing for providing talc minerals; T. B. Zunic for providing tremolite, orthoclase and chlorite; Z. Vardanyan for help with the DNA extractions and library build; and L. B. Levy and D. Skov for their help collecting samples in 2016. This work was prepared in part by Lawrence Livermore National Laboratory under contract DE-AC52-07NA27344; LLNL-JRNL-830653. E.W. thanks St Johns College, Cambridge for providing him with a stimulating environment for scientific thoughts and discussion., Late Pliocene and Early Pleistocene epochs 3.6 to 0.8 million years ago1 had climates resembling those forecasted under future warming2. Palaeoclimatic records show strong polar amplification with mean annual temperatures of 11-19 °C above contemporary values3,4. The biological communities inhabiting the Arctic during this time remain poorly known because fossils are rare5. Here we report an ancient environmental DNA6 (eDNA) record describing the rich plant and animal assemblages of the Kap København Formation in North Greenland, dated to around two million years ago. The record shows an open boreal forest ecosystem with mixed vegetation of poplar, birch and thuja trees, as well as a variety of Arctic and boreal shrubs and herbs, many of which had not previously been detected at the site from macrofossil and pollen records. The DNA record confirms the presence of hare and mitochondrial DNA from animals including mastodons, reindeer, rodents and geese, all ancestral to their present-day and late Pleistocene relatives. The presence of marine species including horseshoe crab and green algae support a warmer climate than today. The reconstructed ecosystem has no modern analogue. The survival of such ancient eDNA probably relates to its binding to mineral surfaces. Our findings open new areas of genetic research, demonstrating that it is possible to track the ecology and evolution of biological communities from two million years ago using ancient eDNA.

Published
2023
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42. Tropostreptus Enghoff 2017

Author

Nielsen, Martin, Margaryan, Ashot, Nielsen, Tejs Lind, Enghoff, Henrik, and Allentoft, Morten E.

Subjects
Arthropoda, Diplopoda, Animalia, Tropostreptus, Biodiversity, Spirostreptidae, Spirostreptida, Taxonomy
Abstract

TROPOSTREPTUS PHYLOGENY AND EVOLUTION Twenty-one of our included mitochondrial genomes belong to Tropostreptus, allowing for a thorough investigation of the evolution of this genus in the Eastern Arc. We observe a clear genetic structure in Tropostreptus, with distinct lineages (both inter- and intraspecific) being defined by the mountain blocks (Fig. 4). This is consistent with previous genetic results of Eastern Arc gene pools (e.g. cat snakes: Gravlund, 2002; chameleons: Tolley et al., 2011; African violets: Dimitrov et al., 2012), where forestadapted species inhabit the montane forests and are absent from the adjacent savannah lowlands. Today, the mountains capture the oceanic winds from the Indian Ocean, which maintains sufficient humidity for dense rain forest to grow, resulting in the forest ‘sky islands’ (Lovett, 1993a, b; Burgess et al., 2007). Until 30 Mya, the Eastern Arc region is thought to have been covered by rain forest (Rodgers, 1998; Couvreur et al., 2008), and an uplifting of the Eastern Arc Mountains is believed to have occurred within the last 7 Myr (although this is debated), changing the whole topography of East Africa (Griffiths, 1993; Ring, 2014; Macgregor, 2015). Climatic and geological fluctuations through time have thus repeatedly affected the forest cover and, presumably, resulted in a multitude of vicariance events when species were isolated in patchy forest remnants (Lovett, 1993a; Sepulchre et al., 2006; Couvreur et al., 2008). For these reasons, the splitting order we observe in the Tropostreptus phylogeny might well reflect forest fragmentation in ancient times. We observe a general trend, whereby northern lineages appear to split off first. The earliest split in Tropostreptus separates the Tropostreptus austerus + Tropostreptus severus lineage from the rest, and the second split separates these two species, today occupying Nguru and Usambara Mountains in the north. A similar intraspecific pattern is evident in more recent splits in Tropostreptus hamatus and Tropostreptus sigmatospinus (Fig. 4), suggesting a repeated pattern of vicariance events occurring first in the north. A separation of species between northern and southern mountains has also been observed in several other Eastern Arc taxa, including amphibians (Blackburn & Measey, 2009), gastropods (Tattersfield et al., 1998) and reptiles (Gravlund, 2002; Tolley et al., 2011), but also in well-dispersing taxa, such as birds (Fjeldså & Bowie, 2008). This indicates a forest retraction southwards during dry periods, resulting in vicariance events, followed by forest expansion and thus northward recolonization of species during periods with higher humidity. Northward migration is also observed in other Eastern Arc species, such as chameleons (Tolley et al., 2011; Ceccarelli et al., 2014). A recent cycle of forest expansion/retraction can explain why Tropostreptus hamatus and Tropostreptus sigmatospinus exist across several of the mountains without having evolved into distinct species yet. Other events have isolated Tropostreptus kipunji in the forest on Mount Rungwe, the most south-westerly occurring species in the Eastern Arc region, in addition to Tropostreptus sigmatospinus in Zanzibar and, potentially, also the Rondo Plateau, from where Tropostreptus has been observed but for which molecular data are still lacking (Enghoff, 2017). Regarding the timing of the species splits (Fig. 5), several major events might have played a role. Around 30 Mya the Antarctic ice sheet started to form (Couvreur et al., 2008), along with rifting that started to occur in northern East Africa (Ring, 2014), possibly initiating the fragmentation of the pan-African forest. Through millions of years, the rifting would continue southwards (Ring, 2014), affecting the topology and possibly related to the forest fragmentation responsible for the divergence of Tropostreptus austerus and Tropostreptus severus observed ~22 Mya. The observed divergence of Tropostreptus hamatus and the split between Tropostreptus austerus and Tropostreptus severus correspond well to the closing of the Tethys Sea (17 Mya), which would have altered ocean currents and, probably, the climate of the area (Couvreur et al., 2008). Likewise, the isolation of the Tropostreptus kipunji lineage corresponds with the uplifting of Mount Rungwe from ~8 Mya (Ring, 2014). Finally, between 5 Mya and today, we observe a radiation in Tropostreptus hamatus and Tropostreptus sigmatospinus (Fig. 5). A reasonable explanation for this is the uplift of the Eastern Arc Mountains, shifting the precipitation from the lowlands to the mountains (Lovett, 1993a, b), in combination with the Antarctic ice sheet forming, thus decreasing global humidity (Polyak et al., 2010). This would lead to the emergence of savannah in the lowlands between the mountains (Sepulchre et al., 2006; Ségalen et al., 2007; Couvreur et al., 2008), isolating the montane forest and limiting migration between populations of forest-restricted species. We emphasize that we have neither good fossil records nor mtDNA mutation rates estimated specifically for millipedes, which is why the split times of our millipede phylogenetic tree should be interpreted with caution. Moreover, comparable studies with dated phylogenies of Eastern Arc species are sparse, hence it is difficult to compare the split times we have estimated with those of other species in the region. Examining two separate studies of chameleons (Kinyongia Tilbury, Tolley & Branch, 2006 and Trioceros Swainson, 1839) with dated phylogenies based on both mitochondrial and nuclear markers did show some correspondence with our dated splits (Tolley et al., 2011; Ceccarelli et al., 2014). Tolley et al. (2011) dated the earliest split between the northern and southern Eastern Arc species to ~28 Mya, and both studies show several radiation events between 5 and 20 Mya, corresponding to the same overall time frame that we are discussing for the millipedes. In contrast, the chameleons display fewer speciation events during the last 5 Myr than the millipedes, perhaps suggesting that the latter have been more susceptible to vicariance during more recent climatic events., Published as part of Nielsen, Martin, Margaryan, Ashot, Nielsen, Tejs Lind, Enghoff, Henrik & Allentoft, Morten E., 2022, Complete mitochondrial genomes from museum specimens clarify millipede evolution in the Eastern Arc Mountains, pp. 924-939 in Zoological Journal of the Linnean Society 196 (2) on pages 933-934, DOI: 10.1093/zoolinnean/zlac058, http://zenodo.org/record/7184588, {"references":["Gravlund P. 2002. Molecular phylogeny of Tornier's cat snake (Crotaphopeltis tornieri), endemic to East African mountain forests: biogeography, vicariance events and problematic species boundaries. Journal of Zoological Systematics and Evolutionary Research 40: 46 - 56.","Tolley KA, Tilbury CR, Measey GJ, Menegon M, Branch WR, Matthee CA. 2011. Ancient forest fragmentation or recent radiation? Testing refugial speciation models in chameleons within an African biodiversity hotspot. Journal of Biogeography 3 8: 1748 - 1760.","Dimitrov D, Nogues-Bravo D, Scharff N. 2012. Why do tropical mountains support exceptionally high biodiversity? The Eastern Arc mountains and the drivers of Saintpaulia diversity. PLoS One 7: e 48908.","Lovett JC. 1993 a. Climatic history and forest distribution in eastern Africa. In: JC Lovett, SK Wasser, eds. Biogeography and ecology of the rain forests of Eastern Africa. Cambridge: Cambridge University Press, 23 - 29.","Burgess N, Butynski TM, Cordeiro NJ, Doggart NH, Fjeldsa J, Howell KM, Kilahama FB, Loader SP, Lovett JC, Mbilinyi B, Menegon M. 2007. The biological importance of the Eastern Arc Mountains of Tanzania and Kenya. Biological Conservation 134: 209 - 231.","Rodgers WA. 1998. An introduction to the conservation of the Eastern Arc Mountains. Journal of East African Natural History 87: 7 - 18.","Couvreur TLP, Chatrou LW, Sosef MSM, Richardson JE. 2008. Molecular phylogenetics reveal multiple tertiary vicariance origins of the African rain forest trees. BMC Biology 6: 54.","Griffiths CJ. 1993. The geological evolution of East Africa. In: JC Lovett, SK Wasser, eds. Biogeography and Ecology of the Rain Forests of Eastern Africa, Cambridge: Cambridge University Press, 9 - 22.","Ring U. 2014. The East African rift system. Austrian Journal of Earth Sciences 107: 132 - 146.","Macgregor D. 2015. History of the development of the East African Rift System: a series of interpreted maps through time. Journal of African Earth Sciences 101: 232 - 252.","Sepulchre P, Ramstein G, Fluteau F, Schuster M, Tiercelin J-J, Brunet M. 2006. Tectonic uplift and Eastern Africa aridification. Science 313: 1419 - 1423.","Blackburn DC, Measey GJ. 2009. Dispersal to or from an African biodiversity hotspot? Molecular Ecology 18: 1904 - 1915.","Tattersfield P, Seddon MB, Meena C. 1998. Ecology and conservation of the land-snails of the Eastern Arc Mountains. Journal of East African Natural History 87: 119 - 138.","Fjeldsa J, Bowie RCK. 2008. New perspectives on the origin and diversification of Africa's forest avifauna. African Journal of Ecology 46: 235 - 247.","Ceccarelli FS, Menegon M, Tolley KA, Tilbury CR, Gower DJ, Laserna MH, Kasahun R, Rodriguez- Prieto A, Hagmann R, Loader SP. 2014. Evolutionary relationships, species delimitation and biogeography of Eastern Afromontane horned chameleons (Chamaeleonidae: Trioceros). Molecular Phylogenetics and Evolution 80: 125 - 136.","Enghoff H. 2017. A new East African genus of spirostreptid millipedes (Diplopoda, Spirostreptida, Spirostreptidae), with notes on their fungal ectoparasite Rickia gigas. Zootaxa 4273: 501 - 530.","Polyak L, Alley RB, Andrews JT, Brigham-Grette J, Cronin TM, Darby DA, Dyke AS, Fitzpatrick JJ, Funder S, Holland M, Jennings AE, Wolff E. 2010. History of sea ice in the Arctic. Quaternary Science Reviews 29: 1757 - 1778.","Segalen L, Lee-Thorp JA, Cerling T. 2007. Timing of C 4 grass expansion across sub-Saharan Africa. Journal of Human Evolution 53: 549 - 559."]}

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2022
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43. The Selection Landscape and Genetic Legacy of Ancient Eurasians

Author

Irving-Pease, Evan K., primary, Refoyo-Martínez, Alba, additional, Ingason, Andrés, additional, Pearson, Alice, additional, Fischer, Anders, additional, Barrie, William, additional, Sjögren, Karl-Göran, additional, Halgren, Alma S., additional, Macleod, Ruairidh, additional, Demeter, Fabrice, additional, Henriksen, Rasmus A., additional, Vimala, Tharsika, additional, McColl, Hugh, additional, Vaughn, Andrew, additional, Stern, Aaron J., additional, Speidel, Leo, additional, Scorrano, Gabriele, additional, Ramsøe, Abigail, additional, Schork, Andrew J., additional, Rosengren, Anders, additional, Zhao, Lei, additional, Kristiansen, Kristian, additional, Sudmant, Peter H., additional, Lawson, Daniel J., additional, Durbin, Richard, additional, Korneliussen, Thorfinn, additional, Werge, Thomas, additional, Allentoft, Morten E., additional, Sikora, Martin, additional, Nielsen, Rasmus, additional, Racimo, Fernando, additional, and Willerslev, Eske, additional

Published
2022
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45. Imputation of ancient genomes

Author

Sousa da Mota, Bárbara, primary, Rubinacci, Simone, additional, Cruz Dávalos, Diana Ivette, additional, Amorim, Carlos Eduardo G., additional, Sikora, Martin, additional, Johannsen, Niels N., additional, Szmyt, Marzena, additional, Włodarczak, Piotr, additional, Szczepanek, Anita, additional, Przybyła, Marcin M., additional, Schroeder, Hannes, additional, Allentoft, Morten E., additional, Willerslev, Eske, additional, Malaspinas, Anna-Sapfo, additional, and Delaneau, Olivier, additional

Published
2022
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46. The Selection Landscape and Genetic Legacy of Ancient Eurasians

Author

Irving-Pease, Evan, Refoyo Martínez, Alba, Ingason, Andrés, Pearson, Alice, Fischer, Anders, Barrie, William, Sjögren, Karl-Göran, Halgren, Alma S., Macleod, Ruairidh, Demeter, Fabrice, Henriksen, Rasmus Henrik Amund, Vimala, Tharsika, McColl, Hugh, Vaughn, Andrew, Stern, Aaron J., Speidel, Leo, Scorrano, Gabriele, Ramsøe, Abigail, Schork, Andrew J., Rosengren, Anders, Zhao, Lei, Kristiansen, Kristian, Sudmant, Peter H., Lawson, Daniel J., Durbin, Richard, Korneliussen, Thorfinn, Werge, Thomas, Allentoft, Morten E., Sikora, Martin, Nielsen, Rasmus, Racimo, Fernando, Willerslev, Eske, Irving-Pease, Evan, Refoyo Martínez, Alba, Ingason, Andrés, Pearson, Alice, Fischer, Anders, Barrie, William, Sjögren, Karl-Göran, Halgren, Alma S., Macleod, Ruairidh, Demeter, Fabrice, Henriksen, Rasmus Henrik Amund, Vimala, Tharsika, McColl, Hugh, Vaughn, Andrew, Stern, Aaron J., Speidel, Leo, Scorrano, Gabriele, Ramsøe, Abigail, Schork, Andrew J., Rosengren, Anders, Zhao, Lei, Kristiansen, Kristian, Sudmant, Peter H., Lawson, Daniel J., Durbin, Richard, Korneliussen, Thorfinn, Werge, Thomas, Allentoft, Morten E., Sikora, Martin, Nielsen, Rasmus, Racimo, Fernando, and Willerslev, Eske

Abstract

The Eurasian Holocene (beginning c. 12 thousand years ago) encompassed some of the most significant changes in human evolution, with far-reaching consequences for the dietary, physical and mental health of present-day populations. Using an imputed dataset of >1600 complete ancient genome sequences, and new computational methods for locating selection in time and space, we reconstructed the selection landscape of the transition from hunting and gathering, to farming and pastoralism across West Eurasia. We identify major selection signals related to metabolism, possibly associated with the dietary shift occurring in this period. We show that the selection on loci such as the FADS cluster, associated with fatty acid metabolism, and the lactase persistence locus, began earlier than previously thought. A substantial amount of selection is also found in the HLA region and other loci associated with immunity, possibly due to the increased exposure to pathogens during the Neolithic, which may explain the current high prevalence of auto-immune disease, such as psoriasis, due to genetic trade-offs. By using ancient populations to infer local ancestry tracks in hundreds of thousands of samples from the UK Biobank, we find strong genetic differentiation among ancient Europeans in loci associated with anthropometric traits and susceptibility to several diseases that contribute to present-day disease burden. These were previously thought to be caused by local selection, but in fact can be attributed to differential genetic contributions from various source populations that are ancestral to present-day Europeans. Thus, alleles associated with increased height seem to have increased in frequency following the Yamnaya migration into northwestern Europe around 5,000 years ago. Alleles associated with increased risk of some mood-related phenotypes are overrepresented in the farmer ancestry component entering Europe from Anatolia around 11,000 years ago, while western hunter-gathere

Published
2022

47. Complete mitochondrial genomes from museum specimens clarify millipede evolution in the Eastern Arc Mountains

Author

Nielsen, Martin, Margaryan, Ashot, Nielsen, Tejs Lind, Enghoff, Henrik, Allentoft, Morten E., Nielsen, Martin, Margaryan, Ashot, Nielsen, Tejs Lind, Enghoff, Henrik, and Allentoft, Morten E.

Abstract

The Eastern Arc Mountains in Tanzania represent a hotspot for biological diversity of global importance. The level of endemism is high, and Eastern Arc biodiversity has been studied extensively in vertebrates and invertebrates, including millipedes. However, millipede evolution is vastly understudied at the molecular level. Therefore, we used next-generation 'shotgun' sequencing to obtain mitochondrial genome sequences of 26 museum specimens, representing six genera and 12 millipede species found across the Eastern Arc Mountains. Bayesian and maximum likelihood methods yielded consistent topologies with high node support, confirming a high level of congruence between molecular and morphological analyses. The only exception was a Tropostreptus sigmatospinus individual from Zanzibar, which was placed outside an otherwise monophyletic group consisting of mainland individuals of the same assumed species. For two species with a distribution across several mountain blocks (Tropostreptus sigmatospinus and Tropostreptus hamatus), each mountain population represents a distinct monophyletic lineage. In contrast, we also observe that distinct species exist sympatrically in the same montane forests, indicative of older speciation events that are not defined by current forest distribution. Our results are important for understanding speciation mechanisms in montane rain forests and highlight that ethanol-preserved invertebrates exhibit a tremendous potential for genomic analyses.

Published
2022

48. Population Genomics of Stone Age Eurasia

Author

Allentoft, Morten, Sikora, Martin, Alba, Refoyo-Martínez, Evan K. Irving-Pease, Fischer, Anders, Barrie, William, Ingason, Andrés, Stenderup, Jesper, Sjögren, Karl-Göran, Pearson, Alice, Sousa da Mota, Bárbara, Schulz Paulsson, Bettina, Halgren, Alma, Macleod, Ruairidh, Schjellerup Jørkov, Marie Louise, Demeter, Fabrice, Novosolov, Maria, Sørensen, Lasse, Nielsen, Poul Otto, Henriksen, Rasmus H.A., Vimala, Tharsika, McColl, Hugh, Margaryan, Ashot, Ilardo, Melissa, Vaughn, Andrew, Mortensen, Morten Fischer, Nielsen, Anne Birgitte, Hede, Mikkel Ulfeldt, Rasmussen, Peter, Vinner, Lasse, Renaud, Gabriel, Stern, Aaron, Theis Zetner Trolle Jensen, Johannsen, Niels Nørkjær, Scorrano, Gabriele, Schroeder, Hannes, Lysdahl, Per, Daisy Ramsøe, Abigail, Skorobogatov, Andrei, Schork, Andrew Joseph, Rosengren, Anders, Ruter, Anthony, Outram, Alan, Timoshenko, Aleksey, Buzhilova, Alexandra, Coppa, Alfredo, Zubova, Alisa, Silva, Ana Maria, Hansen, Anders, Gromov, Andrey, Logvin, Andrey, Gotfredsen, Anne Birgitte, Nielsen, Bjarne Henning, González-Rabanal, Borja, Lalueza-Fox, Carles, McKenzie, Catriona, Gaunitz, Charleen, Blasco, Concepción, Liesau, Corina, Martinez-Labarga, Cristina, Pozdnyakov, Dmitri, Cuenca-Solana, David, Lordkipanidze, David, En’shin, Dmitri, Salazar-García, Domingo, Price, T. Douglas, Borić, Dušan, Kostyleva, Elena, Veselovskaya, Elizaveta, Usmanova, Emma, Cappellini, Enrico, Petersen, Erik Brinch, Kannegaard, Esben, Radina, Francesca, Yediay, Fulya Eylem, Duday, Henri, Gutiérrez-Zugasti, Igor, Potekhina, Inna, Shevnina, Irina, Altinkaya, Isin, Guilaine, Jean, Hansen, Jesper, Tortosa, Joan Emili Aura, Zilhão, João, Vega, Jorge, Pedersen, Kristoffer Buck, Tunia, Krzysztof, Zhao, Lei, Mylnikova, Liudmila, Larsson, Lars, Metz, Laure, Yepiskoposyan, Levon, Pedersen, Lisbeth, Sarti, Lucia, Orlando, Ludovic, Slimak, Ludovic, Klassen, Lutz, Blank, Malou, González-Morales, Manuel, Silvestrini, Mara, Vretemark, Maria, Nesterova, Marina, Rykun, Marina, Rolfo, Mario Federico, Szmyt, Marzena, Przybyła, Marcin, Calattini, Mauro, Sablin, Mikhail, Dobisíková, Miluše, Meldgaard, Morten, Johansen, Morten, Berezina, Natalia, Card, Nick, Saveliev, Nikolai, Poshekhonova, Olga, Rickards, Olga, Lozovskaya, Olga, Gábor, Olivér, Uldum, Otto Christian, Aurino, Paola, Kosintsev, Pavel, Courtaud, Patrice, Ríos, Patricia, Mortensen, Peder, Lotz, Per, Persson, Per, Bangsgaard, Pernille, Damgaard, Peter de Barros, Petersen, Peter Vang, Martinez, Pilar Prieto, Włodarczak, Piotr, Smolyaninov, Roman, Maring, Rikke, Menduiña, Roberto, Badalyan, Ruben, Turin, Ruslan, Vasilyiev, Sergey, Wåhlin, Sidsel, Borutskaya, Svetlana, Skochina, Svetlana, Sørensen, Søren Anker, Andersen, Søren, Jørgensen, Thomas, Serikov, Yuri, Molodin, Vyacheslav, Smrcka, Vaclav, Merz, Victor, Appadurai, Vivek, Moiseyev, Vyacheslav, Magnusson, Yvonne, Kjær, Kurt, Lynnerup, Niels, Lawson, Daniel, Sudmant, Peter, Rasmussen, Simon, Korneliussen, Thorfinn, Durbin, Richard, Nielsen, Rasmus, Delaneau, Olivier, Werge, Thomas, Racimo, Fernando, Kristiansen, Kristian, Willerslev, Eske, Allentoft, Morten, Sikora, Martin, Alba, Refoyo-Martínez, Evan K. Irving-Pease, Fischer, Anders, Barrie, William, Ingason, Andrés, Stenderup, Jesper, Sjögren, Karl-Göran, Pearson, Alice, Sousa da Mota, Bárbara, Schulz Paulsson, Bettina, Halgren, Alma, Macleod, Ruairidh, Schjellerup Jørkov, Marie Louise, Demeter, Fabrice, Novosolov, Maria, Sørensen, Lasse, Nielsen, Poul Otto, Henriksen, Rasmus H.A., Vimala, Tharsika, McColl, Hugh, Margaryan, Ashot, Ilardo, Melissa, Vaughn, Andrew, Mortensen, Morten Fischer, Nielsen, Anne Birgitte, Hede, Mikkel Ulfeldt, Rasmussen, Peter, Vinner, Lasse, Renaud, Gabriel, Stern, Aaron, Theis Zetner Trolle Jensen, Johannsen, Niels Nørkjær, Scorrano, Gabriele, Schroeder, Hannes, Lysdahl, Per, Daisy Ramsøe, Abigail, Skorobogatov, Andrei, Schork, Andrew Joseph, Rosengren, Anders, Ruter, Anthony, Outram, Alan, Timoshenko, Aleksey, Buzhilova, Alexandra, Coppa, Alfredo, Zubova, Alisa, Silva, Ana Maria, Hansen, Anders, Gromov, Andrey, Logvin, Andrey, Gotfredsen, Anne Birgitte, Nielsen, Bjarne Henning, González-Rabanal, Borja, Lalueza-Fox, Carles, McKenzie, Catriona, Gaunitz, Charleen, Blasco, Concepción, Liesau, Corina, Martinez-Labarga, Cristina, Pozdnyakov, Dmitri, Cuenca-Solana, David, Lordkipanidze, David, En’shin, Dmitri, Salazar-García, Domingo, Price, T. Douglas, Borić, Dušan, Kostyleva, Elena, Veselovskaya, Elizaveta, Usmanova, Emma, Cappellini, Enrico, Petersen, Erik Brinch, Kannegaard, Esben, Radina, Francesca, Yediay, Fulya Eylem, Duday, Henri, Gutiérrez-Zugasti, Igor, Potekhina, Inna, Shevnina, Irina, Altinkaya, Isin, Guilaine, Jean, Hansen, Jesper, Tortosa, Joan Emili Aura, Zilhão, João, Vega, Jorge, Pedersen, Kristoffer Buck, Tunia, Krzysztof, Zhao, Lei, Mylnikova, Liudmila, Larsson, Lars, Metz, Laure, Yepiskoposyan, Levon, Pedersen, Lisbeth, Sarti, Lucia, Orlando, Ludovic, Slimak, Ludovic, Klassen, Lutz, Blank, Malou, González-Morales, Manuel, Silvestrini, Mara, Vretemark, Maria, Nesterova, Marina, Rykun, Marina, Rolfo, Mario Federico, Szmyt, Marzena, Przybyła, Marcin, Calattini, Mauro, Sablin, Mikhail, Dobisíková, Miluše, Meldgaard, Morten, Johansen, Morten, Berezina, Natalia, Card, Nick, Saveliev, Nikolai, Poshekhonova, Olga, Rickards, Olga, Lozovskaya, Olga, Gábor, Olivér, Uldum, Otto Christian, Aurino, Paola, Kosintsev, Pavel, Courtaud, Patrice, Ríos, Patricia, Mortensen, Peder, Lotz, Per, Persson, Per, Bangsgaard, Pernille, Damgaard, Peter de Barros, Petersen, Peter Vang, Martinez, Pilar Prieto, Włodarczak, Piotr, Smolyaninov, Roman, Maring, Rikke, Menduiña, Roberto, Badalyan, Ruben, Turin, Ruslan, Vasilyiev, Sergey, Wåhlin, Sidsel, Borutskaya, Svetlana, Skochina, Svetlana, Sørensen, Søren Anker, Andersen, Søren, Jørgensen, Thomas, Serikov, Yuri, Molodin, Vyacheslav, Smrcka, Vaclav, Merz, Victor, Appadurai, Vivek, Moiseyev, Vyacheslav, Magnusson, Yvonne, Kjær, Kurt, Lynnerup, Niels, Lawson, Daniel, Sudmant, Peter, Rasmussen, Simon, Korneliussen, Thorfinn, Durbin, Richard, Nielsen, Rasmus, Delaneau, Olivier, Werge, Thomas, Racimo, Fernando, Kristiansen, Kristian, and Willerslev, Eske

Abstract

Several major migrations and population turnover events during the later Stone Age (after c. 11,000 cal. BP) are believed to have shaped the contemporary population genetic diversity in Eurasia. While the genetic impacts of these migrations have been investigated on regional scales, a detailed understanding of their spatiotemporal dynamics both within and between major geographic regions across Northern Eurasia remains largely elusive. Here, we present the largest shotgun-sequenced genomic dataset from the Stone Age to date, representing 317 primarily Mesolithic and Neolithic individuals from across Eurasia, with associated radiocarbon dates, stable isotope data, and pollen records. Using recent advances, we imputed >1,600 ancient genomes to obtain accurate diploid genotypes, enabling previously unachievable fine-grained population structure inferences. We show that 1) Eurasian Mesolitic hunter-gatherers were more genetically diverse than previously known, and deeply divergent between the west and the east; 2) Hitherto genetically undescribed huntergatherers from the Middle Don region contributed significant ancestry to the later Yamnaya steppe pastoralists; 3) The genetic impact of the transition from Mesolithic hunter-gatherers to Neolithic farmers was highly distinct, east and west of a “Great Divide” boundary zone extending from the Black Sea to the Baltic, with large-scale shifts in genetic ancestry to the west. This include an almost complete replacement of hunter-gatherers in Denmark, but no substantial shifts during the same period further to the east; 4) Within-group relatedness changes substantially during the Neolithic transition in the west, where clusters of Neolithic farmer-associated individuals show overall reduced relatedness, while genetic relatedness remains high until ~4,000 BP in the east, consistent with a much longer persistence of smaller localised hunter-gatherer groups; 5) A fastpaced second major genetic transformation beginning around 5,0

Published
2022

49. Applications of environmental DNA (eDNA) in agricultural systems:Current uses, limitations and future prospects

Author

Kestel, Joshua H., Field, David L., Bateman, Philip W., White, Nicole E., Allentoft, Morten E., Hopkins, Anna J. M., Gibberd, Mark, Nevill, Paul, Kestel, Joshua H., Field, David L., Bateman, Philip W., White, Nicole E., Allentoft, Morten E., Hopkins, Anna J. M., Gibberd, Mark, and Nevill, Paul

Abstract

Global food production, food supply chains and food security are increasingly stressed by human population growth and loss of arable land, becoming more vulnerable to anthropogenic and environmental perturbations. Numerous mutualistic and antagonistic species are interconnected with the cultivation of crops and livestock and these can be challenging to identify on the large scales of food production systems. Accurate identifications to capture this diversity and rapid scalable monitoring are necessary to identify emerging threats (i.e. pests and pathogens), inform on ecosystem health (i.e. soil and pollinator diversity), and provide evidence for new management practices (i.e. fertiliser and pesticide applications). Increasingly, environmental DNA (eDNA) is providing rapid and accurate classifications for specific organisms and entire species assemblages in substrates ranging from soil to air. Here, we aim to discuss how eDNA is being used for monitoring of agricultural ecosystems, what current limitations exist, and how these could be managed to expand applications into the future. In a systematic review we identify that eDNA-based monitoring in food production systems accounts for only 4 % of all eDNA studies. We found that the majority of these eDNA studies target soil and plant substrates (60 %), predominantly to identify microbes and insects (60 %) and are biased towards Europe (42 %). While eDNA-based monitoring studies are uncommon in many of the world's food production systems, the trend is most pronounced in emerging economies often where food security is most at risk. We suggest that the biggest limitations to eDNA for agriculture are false negatives resulting from DNA degradation and assay biases, as well as incomplete databases and the interpretation of abundance data. These require in silico, in vitro, and in vivo approaches to carefully design, test and apply eDNA monitoring for reliable and accurate taxonomic identifications. We explore future oppor

Published
2022

50. Genomic ancestry, diet and microbiomes of Upper Palaeolithic hunter-gatherers from San Teodoro cave (Sicily, Italy)

Author

Scorrano, Gabriele, Nielsen, Sofie Holtsmark, Vetro, Domenico Lo, Sawafuji, Rikai, Mackie, Meaghan, Margaryan, Ashot, Fotakis, Anna K., Martínez-Labarga, Cristina, Fabbri, Pier Francesco, Allentoft, Morten E., Carra, Marialetizia, Martini, Fabio, Rickards, Olga, Olsen, Jesper V., Pedersen, Mikkel Winther, Cappellini, Enrico, Sikora, Martin, Scorrano, Gabriele, Nielsen, Sofie Holtsmark, Vetro, Domenico Lo, Sawafuji, Rikai, Mackie, Meaghan, Margaryan, Ashot, Fotakis, Anna K., Martínez-Labarga, Cristina, Fabbri, Pier Francesco, Allentoft, Morten E., Carra, Marialetizia, Martini, Fabio, Rickards, Olga, Olsen, Jesper V., Pedersen, Mikkel Winther, Cappellini, Enrico, and Sikora, Martin

Abstract

Recent improvements in the analysis of ancient biomolecules from human remains and associated dental calculus have provided new insights into the prehistoric diet and genetic diversity of our species. Here we present a multi-omics study, integrating metagenomic and proteomic analyses of dental calculus, and human ancient DNA analysis of the petrous bones of two post-Last Glacial Maximum (LGM) individuals from San Teodoro cave (Italy), to reconstruct their lifestyle and the post-LGM resettlement of Europe. Our analyses show genetic homogeneity in Sicily during the Palaeolithic, representing a hitherto unknown Italian genetic lineage within the previously identified Villabruna cluster. We argue that this lineage took refuge in Italy during the LGM, followed by a subsequent spread to central-western Europe. Analysis of dental calculus showed a diet rich in animal proteins which is also reflected on the oral microbiome composition. Our results demonstrate the power of this approach in the study of prehistoric humans and will enable future research to reach a more holistic understanding of the population dynamics and ecology.

Published
2022

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