164 results on '"Alanio, Alexandre"'
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2. Laboratory practices for the diagnosis and management of mucormycosis in France, 2024
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Millon, Laurence, Botterel, Françoise, Bonhomme, Julie, Valot, Stéphane, Poirier, Philippe, Durieux, Marie-Fleur, Bigot, Jeanne, Desoubeaux, Guillaume, Chesnais, Adélaïde, Morio, Florent, Pihet, Marc, Brunet, Kévin, Bellanger, Anne-Pauline, Imbert, Sébastien, Nevez, Gilles, Gal, Solène Le, Bourgeois, Nathalie, Debourgogne, Anne, Cornu, Marjorie, Persat, Florence, Hasseine, Lilia, Bougnoux, Marie-Elisabeth, Brun, Sophie, Cornet, Muriel, Favennec, Loïc, Gargala, Gilles, Bonnal, Christine, Gangneux, Jean-Pierre, Alanio, Alexandre, Iriart, Xavier, Mahinc, Caroline, Chouaki, Taieb, Paugam, André, Letscher-Bru, Valérie, and Dannaoui, Eric
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- 2024
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3. Reappraising Cladophialophora bantiana phaeohyphomycosis in France: retrospective nation-based study
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Lortholary, Olivier, Garcia-Hermoso, Dea, Sturny-Leclère, Aude, Sitbon, Karine, Nourrisson, Céline, Letscher-Bru, Valérie, Desbois-Nogard, Nicole, Bani-Sadr, Ferouze, Bastides, Frédéric, Bienvenu, Boris, Cordier, Camille, Coste, Anne, Danion, François, Dégot, Tristan, Delarbre, David, Fekkar, Arnaud, Garcie, Christophe, Garrouste, Cyril, Gits-Muselli, Maud, Guemas, Emilie, Huguenin, Antoine, Janvier, Frédéric, Kamar, Nassim, Kervinio, Cyril, Le Gal, Solène, Lesens, Olivier, Machouart, Marie, Persat, Florence, Picot, Sandrine, Rouze, Anahita, Ranque, Stéphane, Ruch, Yvon, Saada, Matthieu, Stabler, Sarah, Alanio, Alexandre, Lanternier, Fanny, and Desoubeaux, Guillaume
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- 2024
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4. WHO global research priorities for antimicrobial resistance in human health
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Aanensen, David, Alanio, Alexandre, Alastruey-Izquierdo, Ana, Alemayehu, Tinsae, Al-Hasan, Majdi, Allegaert, Karel, Al-Maani, Amal Saif, Al-Salman, Jameela, Alshukairi, Abeer Nizar, Amir, Afreenish, Applegate, Tanya, Araj, George F, Villalobos, Marlen Arce, Årdal, Christine, Ashiru-Oredope, Diane, Ashley, Elizabeth A, Babin, François-Xavier, Bachmann, Laura H, Bachmann, Till, Baker, Kate Susan, Balasegaram, Manica, Bamford, Colleen, Baquero, Fernando, Barcelona, Laura Isabel, Bassat, Quique, Bassetti, Matteo, Basu, Sulagna, Beardsley, Justin, Vásquez, Grey Benoit, Berkley, James A, Bhatnagar, Anuj K, Bielicki, Julia, Bines, Julie, Bongomin, Felix, Bonomo, Robert A, Bradley, John S, Bradshaw, Catriona, Brett, Ana, Brink, Adrian, Brown, Colin, Brown, Jeremy, Buising, Kirsty, Carson, Carolee, Carvalho, Anna Cristina, Castagnola, Elio, Cavaleri, Marco, Cecchini, Michele, Chabala, Chishala, Chaisson, Richard E, Chakrabarti, Arunaloke, Chandler, Clare, Chandy, Sujith John, Charani, Esmita, Chen, Lisa, Chiara, Francesca, Chowdhary, Anuradha, Chua, Arlene, Chuki, Pem, Chun, Doo Ryeon, Churchyard, Gavin, Cirillo, Daniela, Clack, Lauren, Coffin, Susan E, Cohn, Jennifer, Cole, Michelle, Conly, John, Cooper, Ben, Corso, Alejandra, Cosgrove, Sara E, Cox, Helen, Daley, Charles L, Darboe, Saffiatou, Darton, Tom, Davies, Gerry, de Egea, Viviana, Dedeić-Ljubović, Amela, Deeves, Miranda, Denkinger, Claudia, Dillon, Jo-Anne R, Dramowski, Angela, Eley, Brian, Roberta Esposito, Susanna Maria, Essack, Sabiha Y, Farida, Helmia, Farooqi, Joveria, Feasey, Nicholas, Ferreyra, Cecilia, Fifer, Helen, Finlayson, Heather, Frick, Mike, Gales, Ana Cristina, Galli, Luisa, Gandra, Sumanth, Gerber, Jeffrey S, Giske, Christian, Gordon, Bruce, Govender, Nelesh, Guessennd, Nathalie, Guindo, Ibrehima, Gurbanova, Elmira, Gwee, Amanda, Hagen, Ferry, Harbarth, Stephan, Haze, John, Heim, Jutta, Hendriksen, Rene, Heyderman, Robert Simon, Holt, Kathryn Elizabeth, Hönigl, Martin, Hook, Edward W, Hope, William, Hopkins, Heidi, Hughes, Gwenda, Ismail, Ghada, Issack, Mohammad Iqbal, Jacobs, Jan, Jasovský, Dušan, Jehan, Fyeza, Pearson, Antonieta Jimenez, Jones, Makoto, Joshi, Mohan P, Kapil, Arti, Kariuki, Samuel, Karkey, Abhilasha, Kearns, Gregory L, Keddy, Karen Helena, Khanna, Nina, Kitamura, Akiko, Kolho, Kaija-Leena, Kontoyiannis, Dimitrios P, Kotwani, Anita, Kozlov, Roman S, Kranzer, Katharina, Kularatne, Ranmini, Lahra, Monica M, Langford, Bradley J, Laniado-Laborin, Rafael, Larsson, Joakim, Lass-Flörl, Cornelia, Le Doare, Kirsty, Lee, Hyukmin, Lessa, Fernanda, Levin, Anna S, Limmathurotsakul, Direk, Lincopan, Nilton, Lo Vecchio, Andrea, Lodha, Rakesh, Loeb, Mark, Longtin, Yves, Lye, David Chien, Mahmud, Asif Mujtaba, Manaia, Célia, Manderson, Lenore, Mareković, Ivana, Marimuthu, Kalisvar, Martin, Irene, Mashe, Tapfumanei, Mei, Zeng, Meis, Jacques F, Lyra Tavares De Melo, Flávio Augusto, Mendelson, Marc, Miranda, Angelica Espinosa, Moore, David, Morel, Chantal, Moremi, Nyambura, Moro, Maria Luisa, Moussy, Francis, Mshana, Stephen, Mueller, Arno, Ndow, Francis J, Nicol, Mark, Nunn, Andrew, Obaro, Stephen, Obiero, Christina W, Okeke, Iruka N, Okomo, Uduak, Okwor, Tochi J, Oladele, Rita, Omulo, Sylvia, Ondoa, Pascale, Ortellado de Canese, Juana Medarda, Ostrosky-Zeichner, Luis, Padoveze, Maria Clara, Pai, Madhukar, Park, Benjamin, Parkhill, Julian, Parry, Christopher M, Peeling, Rosanna, Sobreira Vieira Peixe, Luísa Maria, Perovic, Olga, Pettigrew, Melinda M, Principi, Nicola, Pulcini, Céline, Puspandari, Nelly, Rawson, Timothy, Reddy, Denasha Lavanya, Reddy, Kessendri, Redner, Paulo, Rodríguez Tudela, Juan Luis, Rodríguez-Baño, Jesús, Van Katwyk, Susan Rogers, Roilides, Emmanuel, Rollier, Christine, Rollock, Leslie, Ronat, Jean-Baptiste, Ruppe, Etienne, Sadarangani, Manish, Salisbury, David, Salou, Mounerou, Samison, Luc Hervé, Sanguinetti, Maurizio, Sartelli, Massimo, Schellack, Natalie, Schouten, Jeroen, Schwaber, Mitchell J, Seni, Jeremiah, Senok, Abiola, Shafer, William M, Shakoor, Sadia, Sheppard, Donald, Shin, Jong-Hee, Sia, Sonia, Sievert, Dawn, Singh, Ishwar, Singla, Rupak, Skov, Robert Leo, Soge, Olusegun O, Sprute, Rosanne, Srinivasan, Arjun, Srinivasan, Subasree, Sundsfjord, Arnfinn, Tacconelli, Evelina, Tahseen, Sabira, Tangcharoensathien, Viroj, Tängdén, Thomas, Thursky, Karin, Thwaites, Guy, Tigulini de Souza Peral, Renata, Tong, Deborah, Tootla, Hafsah Deepa, Tsioutis, Constantinos, Turner, Katy M, Turner, Paul, Omar, Shaheed Vally, van de Sande, Wendy WJ, van den Hof, Susan, van Doorn, Rogier, Veeraraghavan, Balaji, Verweij, Paul, Wahyuningsih, Retno, Wang, Hui, Warris, Adilia, Weinstock, Hillard, Wesangula, Evelyn, Whiley, David, White, Peter J, Williams, Phoebe, Xiao, Yonghong, Moscoso, Martin Yagui, Yang, Hsu Li, Yoshida, Sachiyo, Yu, Yunsong, Żabicka, Dorota, Zignol, Matteo, Rudan, Igor, Bertagnolio, Silvia, Dobreva, Zlatina, Centner, Chad M, Olaru, Ioana Diana, Donà, Daniele, Burzo, Stefano, Huttner, Benedikt D, Chaillon, Antoine, Gebreselassie, Nebiat, Wi, Teodora, Hasso-Agopsowicz, Mateusz, Allegranzi, Benedetta, Sati, Hatim, Ivanovska, Verica, Kothari, Kavita U, Balkhy, Hanan H, Cassini, Alessandro, Hamers, Raph L, and Weezenbeek, Kitty Van
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- 2024
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5. Epidemiology and prognostic factors of mucormycosis in France (2012–2022): a cross-sectional study nested in a prospective surveillance programme
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Gouzien, Laura, Che, Didier, Cassaing, Sophie, Lortholary, Olivier, Letscher-Bru, Valérie, Paccoud, Olivier, Obadia, Thomas, Morio, Florent, Moniot, Maxime, Cateau, Estelle, Bougnoux, Marie Elisabeth, Chouaki, Taieb, Hasseine, Lilia, Desoubeaux, Guillaume, Gautier, Cecile, Mahinc-Martin, Caroline, Huguenin, Antoine, Bonhomme, Julie, Sitbon, Karine, Durand, Julien, Alanio, Alexandre, Millon, Laurence, Garcia-Hermoso, Dea, and Lanternier, Fanny
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- 2024
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6. Cryptococcal meningitis and cerebral vasculitis in a patient with primary intestinal lymphangiectasia: a case report
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Mathurin, Martin, Devatine, Sandra, Kopp-Derouet, Aude, Guillonnet, Antoine, Alanio, Alexandre, Lourenco, Nelson, Manda, Victoria, Delcey, Véronique, Molina, Jean-Michel, and Sellier, Pierre
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- 2023
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7. Les infections à champignons dimorphiques en 2024
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Melenotte, Cléa, Françoise, Ugo, Sturny, Aude, Hermoso, Dea Garcia, Alanio, Alexandre, Lanternier, Fanny, and Lortholary, Olivier
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- 2024
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8. Candida auris : de quelle ampleur est la menace ?
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Desoubeaux, Guillaume and Alanio, Alexandre
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- 2024
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9. Global guideline for the diagnosis and management of cryptococcosis: an initiative of the ECMM and ISHAM in cooperation with the ASM
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Chang, Christina C, Harrison, Thomas S, Bicanic, Tihana A, Chayakulkeeree, Methee, Sorrell, Tania C, Warris, Adilia, Hagen, Ferry, Spec, Andrej, Oladele, Rita, Govender, Nelesh P, Chen, Sharon C, Mody, Christopher H, Groll, Andreas H, Chen, Yee-Chun, Lionakis, Michail S, Alanio, Alexandre, Castañeda, Elizabeth, Lizarazo, Jairo, Vidal, José E, Takazono, Takahiro, Hoenigl, Martin, Alffenaar, Jan-Willem, Gangneux, Jean-Pierre, Soman, Rajeev, Zhu, Li-Ping, Bonifaz, Alexandro, Jarvis, Joseph N, Day, Jeremy N, Klimko, Nikolai, Salmanton-García, Jon, Jouvion, Grégory, Meya, David B, Lawrence, David, Rahn, Sebastian, Bongomin, Felix, McMullan, Brendan J, Sprute, Rosanne, Nyazika, Tinashe K, Beardsley, Justin, Carlesse, Fabianne, Heath, Christopher H, Ayanlowo, Olusola O, Mashedi, Olga M, Queiroz-Telles Filho, Flavio, Hosseinipour, Mina C, Patel, Atul K, Temfack, Elvis, Singh, Nina, Cornely, Oliver A, Boulware, David R, Lortholary, Olivier, Pappas, Peter G, and Perfect, John R
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- 2024
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10. Features of cryptococcosis among 652 HIV-seronegative individuals in France: a cross-sectional observational study (2005-2020)
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Brieu, N., Durand, C., Bertei, D., Bouchara, J.P., Pihet, M., Bland, S., Bru, J.P., Pulik, M., Le Turdu, F., Lefrand C, H., Ferrand, M., Larrouy, M., Millon, L., Delhaes, L., Imbert, S., Accoceberry, I., Bachelier, M.N., Nevez, G., Quinio, D., Le Coustumier, A., Carmagnol, F., Rivière, B., Boex, P., Podac, B., Moniot, M., Nourrisson, C., Augereau, O., Emond, J.P., Belkacem-Belkaki, G., Bacri, J.L., Berthelot, G., Dalle, F., Vallee, E., Bizet, J., Noussair, L., Herrmann, J.L., Maubon, D., Brocard, C., Guiffault, P., Layet, A., Morel, A., Angoulvant, A., Penn, P., Gigandon, A., Sendid, B., Cornu, M., Darde, M.L., Jaccard, A., Bouteille, B., Azjenberg, D., Prades, N., Bienvenu, A.L., Benoit-Cattin, T., Fiacre, A., Levy, S., Pitsch, A., Kiefer, M.H., Debourgogne, A., Moquet, O., Colot, J., Courtellemont, L., Poisson, D., Laurens, V., Kauffmann-Lacroix, C., Martres, P., Gargala, G., Godineau, N., Picot, S., Chassagne, C., Djibo, N., Devallière, R., Sabou, M., Camin-Ravenne, A.M., Bissuel, F., Janvier, F., Aubert, X., Chadapaud, S., Delbeck, X., Lafeuillade, A., Raoult, X., Baclet, V., Coignard, C., Mouton, Y., Ravaux, I., Eloy, C., Fur, A., Rezzouk, L., Mazards, E., Eloy, O., Chachaty, E., Mihaila, L., Dellion, S., Patey, O., Thouvenot, A., Limousin, L., Paugam, A., Desplaces, N., Raguin, G., Sitterlé, E., Blaize, M., Gits-Muselli, M., Hennequin, C., Poirot, J.L., Bretagne, S., Lacroix, Claire, Hamane, Samia, Paccoud, Olivier, Desnos-Ollivier, Marie, Persat, Florence, Demar, Magalie, Boukris-Sitbon, Karine, Bellanger, Anne-Pauline, Bonhomme, Julie, Bonnal, Christine, Botterel, Françoise, Bougnoux, Marie-Elisabeth, Brun, Sophie, Cassaing, Sophie, Cateau, Estelle, Chouaki, Taieb, Cornet, Muriel, Dannaoui, Eric, Desbois-Nogard, Nicole, Durieux, Marie-Fleur, Favennec, Loïc, Fekkar, Arnaud, Gabriel, Frederic, Gangneux, Jean-Pierre, Guitard, Juliette, Hasseine, Lilia, Huguenin, Antoine, Le Gal, Solène, Letscher-Bru, Valérie, Mahinc, Caroline, Morio, Florent, Nicolas, Muriel, Poirier, Philippe, Ranque, Stéphane, Roosen, Gabrielle, Rouges, Célia, Roux, Anne-Laure, Sasso, Milène, Alanio, Alexandre, Lortholary, Olivier, and Lanternier, Fanny
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- 2024
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11. Development and validation of quantitative PCR assays for HIV-associated cryptococcal meningitis in sub-Saharan Africa: a diagnostic accuracy study
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Goodall, J, Mawoko, N, Milburn, J, Mmipi, R, Muthoga, C, Ponatshego, P, Rulaganyang, I, Seatla, K, Tlhako, N, Tsholo, K, April, S, Bekiswa, A, Boloko, L, Bookholane, H, Crede, T, Davids, L, Goliath, R, Hlungulu, S, Hoffman, R, Kyepa, H, Masina, N, Maughan, D, Mnguni, T, Moosa, S, Morar, T, Mpalali, M, Naude, J, Oliphant, I, Sayed, S, Sebesho, L, Shey, M, Swanepoel, L, Chasweka, M, Chimang’anga, W, Chimphambano, T, Dziwani, E, Gondwe, E, Kadzilimbile, A, Kateta, S, Kossam, E, Kukacha, C, Lipenga, B, Ndaferankhande, J, Ndalama, M, Shah, R, Singini, A, Stott, K, Zambasa, A, Banda, T, Chikaonda, T, Chitulo, G, Chiwoko, L, Chome, N, Gwin, M, Kachitosi, T, Kamanga, B, Kazembe, M, Kumwenda, E, Kumwenda, M, Maya, C, Mhango, W, Mphande, C, Msumba, L, Munthali, T, Ngoma, D, Nicholas, S, Simwinga, L, Stambuli, A, Tegha, G, Zambezi, J, Ahimbisibwe, C, Akampurira, A, Alice, A, Cresswell, F, Gakuru, J, Kiiza, D, Kisembo, J, Kwizera, R, Kugonza, F, Laker, E, Luggya, T, Lule, A, Musubire, A, Muyise, R, Namujju, O, Ndyetukira, J, Nsangi, L, Okirwoth, M, Sadiq, A, Tadeo, K, Tukundane, A, Williams, D, Atwine, L, Buzaare, P, Collins, M, Emily, N, Inyakuwa, C, Kariisa, S, Mwesigye, J, Niwamanya, S, Rodgers, A, Rukundo, J, Rwomushana, I, Ssemusu, M, Stead, G, Boyd, K, Gondo, S, Kufa, P, Makaha, E, Moyo, C, Mtisi, T, Mudzingwa, S, Mwarumba, T, Zinyandu, T, Dromer, F, Johnstone, P, Hafeez, S, Mbangiwa, Tshepiso, Sturny-Leclère, Aude, Lechiile, Kwana, Kajanga, Cheusisime, Boyer-Chammard, Timothée, Hoving, Jennifer C, Leeme, Tshepo, Moyo, Melanie, Youssouf, Nabila, Lawrence, David S, Mwandumba, Henry, Mosepele, Mosepele, Harrison, Thomas S, Jarvis, Joseph N, Lortholary, Olivier, and Alanio, Alexandre
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- 2024
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12. Cost-effectiveness of single, high-dose, liposomal amphotericin regimen for HIV-associated cryptococcal meningitis in five countries in sub-Saharan Africa: an economic analysis of the AMBITION-cm trial
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Goodall, Jack, Lechiile, Kwana, Mawoko, Norah, Mbangiwa, Tshepiso, Milburn, James, Mmipi, Refilwe, Ponatshego, Ponego, Rulaganyang, Ikanyang, Seatla, Kaelo, Siamisang, Keatlaretse, Tlhako, Nametso, Tsholo, Katlego, April, Samantha, Bekiswa, Abulele, Boloko, Linda, Bookholane, Hloni, Crede, Thomas, Davids, Lee-Ann, Goliath, Rene, Hlungulu, Siphokazi, Hoffman, Regina, Kyepa, Henriette, Masina, Noma, Maughan, Deborah, Mnguni, Trevor, Moosa, Sumaiyya, Morar, Tania, Mpalali, Mkanyiseli, Naude, Jonathan, Oliphant, Ida, Singh, Achita, Sayed, Sumaya, Sebesho, Leago, Shey, Muki, Swanepoel, Loraine, Chasweka, Madalitso, Chimang'anga, Wezi, Chimphambano, Tipatseni, Gondwe, Ebbie, Mzinganjira, Henry, Kadzilimbile, Aubrey, Kateta, Steven, Kossam, Evelyn, Kukacha, Christopher, Lipenga, Bright, Ndaferankhande, John, Ndalama, Maureen, Shah, Reya, Singini, Andreas, Stott, Katherine, Zambasa, Agness, Banda, Towera, Chikaonda, Tarsizio, Chitulo, Gladys, Chiwoko, Lorren, Chome, Nelecy, Gwin, Mary, Kachitosi, Timothy, Kamanga, Beauty, Kazembe, Mussah, Kumwenda, Emily, Kumwenda, Masida, Maya, Chimwemwe, Mhango, Wilberforce, Mphande, Chimwemwe, Msumba, Lusungu, Munthali, Tapiwa, Ngoma, Doris, Nicholas, Simon, Simwinga, Lusayo, Stambuli, Anthony, Tegha, Gerald, Zambezi, Janet, Ahimbisibwe, Cynthia, Akampurira, Andrew, Alice, Anamudde, Cresswell, Fiona, Gakuru, Jane, Kagimu, Enock, Kasibante, John, Kiiza, Daniel, Kisembo, John, Kwizera, Richard, Kugonza, Florence, Laker, Eva, Luggya, Tonny, Lule, Andrew, Musubire, Abdu, Muyise, Rhona, Namujju, Carol Olivie, Ndyetukira, Jane Francis, Nsangi, Laura, Okirworth, Michael, Rhein, Joshua, Rutakingirwa, Morris K, Sadiq, Alisat, Ssebambulidde, Kenneth, Tadeo, Kiiza, Tukundane, Asmus, Atwine, Leo, Buzaare, Peter, Collins, Muganzi, Emily, Ninsima, Inyakuwa, Christine, Kariisa, Samson, Mwesigye, James, Nuwamanya, Simpson, Rodgers, Ankunda, Rukundo, Joan, Rwomushana, Irene, Ssemusu, Mike, Stead, Gavin, Boyd, Kathyrn, Gondo, Secrecy, Kufa, Prosper, Makaha, Edward, Moyo, Colombus, Mtisi, Takudzwa, Mudzinga, Shepherd, Mutata, Constantine, Mwarumba, Taddy, Zinyandu, Tawanda, Alanio, Alexandre, Dromer, Francoise, Lortholary, Olivier, Sturny-Leclere, Aude, Griffin, Philippa, Hafeez, Sophia, Loyse, Angela, van Widenfelt, Erik, Lawrence, David S, Muthoga, Charles, Meya, David B, Tugume, Lillian, Williams, Darlisha, Rajasingham, Radha, Boulware, David R, Mwandumba, Henry C, Moyo, Melanie, Dziwani, Eltas N, Maheswaran, Hendramoorthy, Kanyama, Cecilia, Hosseinipour, Mina C, Chawinga, Chimwemwe, Meintjes, Graeme, Schutz, Charlotte, Comins, Kyla, Bango, Funeka, Muzoora, Conrad, Jjunju, Samuel, Nuwagira, Edwin, Mosepele, Mosepele, Leeme, Tshepo, Ndhlovu, Chiratidzo E, Hlupeni, Admire, Shamu, Shepherd, Boyer-Chammard, Timothée, Molloy, Síle F, Youssouf, Nabila, Chen, Tao, Shiri, Tinevimbo, Jaffar, Shabbar, Harrison, Thomas S, Jarvis, Joseph N, and Niessen, Louis W
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- 2022
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13. The role of glycosylphosphatidylinositol (gpi) anchored proteins in Cryptococcusneoformans
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Snelders, Eveline, Moyrand, Frédérique, Sturny-Leclère, Aude, Vernel-Pauillac, Frédérique, Volant, Stevenn, Janbon, Guilhem, and Alanio, Alexandre
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- 2022
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14. Auranofin is active against Histoplasma capsulatum and reduces the expression of virulence-related genes.
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de Abreu Almeida, Marcos, Baeza, Lilian Cristiane, Silva, Leandro B. R., Bernardes-Engemann, Andréa Reis, Almeida-Silva, Fernando, Coelho, Rowena Alves, de Andrade, Iara Bastos, Corrêa-Junior, Dario, Frases, Susana, Zancopé-Oliveira, Rosely Maria, Alanio, Alexandre, Taborda, Carlos Pelleschi, and Almeida-Paes, Rodrigo
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TENEBRIO molitor ,DRUG efficacy ,YEAST fungi ,AURANOFIN ,MYCOSES ,HISTOPLASMOSIS - Abstract
Background: Auranofin is an approved anti-rheumatic drug that has a broad-range inhibitory action against several microorganisms, including human pathogenic fungi. The auranofin activity against Histoplasma capsulatum, the dimorphic fungus that causes histoplasmosis, has not been properly addressed. Since there are few therapeutic options for this life-threatening systemic mycosis, this study evaluated the effects of auranofin on H. capsulatum growth and expression of virulence factors. Methodology/principal findings: Minimal inhibitory and fungicidal concentrations (MIC and MFC, respectively) of auranofin against 15 H. capsulatum strains with distinct genetic backgrounds were determined using the yeast form of the fungus and a microdilution protocol. Auranofin activity was also assessed on a macrophage model of infection and on a Tenebrio molitor invertebrate animal model. Expression of virulence-related genes was compared between auranofin treated and untreated H. capsulatum yeast cells using a quantitative PCR assay. Auranofin affected the growth of different strains of H. capsulatum, with MIC and MFC values ranging from 1.25 to 5.0 μM and from 2.5 to >10 μM, respectively. Auranofin was able to kill intracellular H. capsulatum yeast cells and conferred protection against the fungus in the experimental animal model of infection. Moreover, the expression of catalase A, HSP70, superoxide dismutase, thioredoxin reductase, serine proteinase, cytochrome C peroxidase, histone 2B, formamidase, metallopeptidase, Y20 and YPS3 proteins were reduced after six hours of auranofin treatment. CONCLUSIONS/SIGNIFICANCE: Auranofin is fungicidal against H. capsulatum and reduces the expression of several virulence-related genes, which makes this anti-rheumatic drug a good candidate for new medicines against histoplasmosis. Author summary: Histoplasmosis is a serious disease caused by the fungus Histoplasma capsulatum, which can be life-threatening and has limited treatment options. In this study, we investigated the potential of auranofin, a drug originally approved for treating rheumatoid arthritis, to combat H. capsulatum. We tested auranofin against different strains of the fungus and found that it effectively inhibited fungal growth. This drug was also effective in killing the fungus within cells from the immune system and provided protection in an experimental animal model. Furthermore, auranofin reduced the expression of several genes associated with the fungus's ability to cause disease. These genes are involved in critical functions such as detoxifying harmful substances and maintaining cellular structures. By suppressing these genes, auranofin hampers the fungus's ability to survive and cause infection. These findings suggest that auranofin could be repurposed as a treatment for histoplasmosis, offering a new avenue for therapy against this challenging infection. This research is significant because it highlights the potential of existing drugs to treat other diseases, broadening our arsenal of available treatments and contributing to better healthcare solutions. [ABSTRACT FROM AUTHOR]
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- 2024
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15. Strengths and limitations of molecular diagnostics for Pneumocystis jirovecii pneumonia.
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Brown, Lottie, Alanio, Alexandre, Cruciani, Mario, Barnes, Rosemary, Donnelly, J. Peter, Loeffler, Juergen, Rautemaa-Richardson, Riina, and White, P. Lewis
- Abstract
Introduction: While Pneumocystis pneumonia (PcP) remains a major AIDS-defining disease, the majority of cases of PcP now present in the HIV-negative cohort, causing significant mortality. PcP PCR diagnosis is not novel, and the optimal route of diagnosis remains unclear, with an imperfect reference method and complexity in result interpretation for alternative tests. Areas covered: This extensive review utilizing a literature search underpinning a recent systematic review/meta-analysis discusses the technical and clinical performance of PcP PCR, the added benefits of PCR testing, future aspects/considerations, and how PCR may be best used in clinical algorithms to provide a probability of PcP. Expert opinion: Given the current imperfect reference test for PcP, an alternative would be beneficial. Concerns over PcP PCR generating false positive results are valid but can be resolved by using positivity thresholds that drive specificity. Unfortunately, the extensive range of PCR assays complicates the provision of a PCR reference method. Combination testing incorporating PCR and B-D-Glucan, along with clinical and host risk factors, is key to understanding the individual probability of PcP. It is critical that access to PcP PCR testing is improved through technical and logistical development. Conversely, syndromic approaches including PcP need to be fully evaluated. [ABSTRACT FROM AUTHOR]
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- 2024
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16. Emergence of Difficult-to-Treat Tinea Corporis Caused by Trichophyton mentagrophytes Complex Isolates, Paris, France
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Delliere, Sarah, Joannard, Brune, Benderdouche, Mazouz, Mingui, Anselme, Gits-Muselli, Maud, Hamane, Samia, Alanio, Alexandre, Petit, Antoine, Gabison, Germaine, Bagot, Martine, and Bretagne, Stephane
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Drug resistance in microorganisms -- Causes of -- Genetic aspects ,Ringworm -- Drug therapy -- Causes of ,Dermatophytes -- Identification and classification -- Genetic aspects ,Health - Abstract
Dermatophytes are filamentous keratinophilic fungi responsible for superficial fungal diseases involving the skin, hair, or nails. On the basis of the site of the lesions, conditions are called tinea corporis, [...]
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- 2022
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17. COVID-19-associated mixed mold infection: A case report of aspergillosis and mucormycosis and a literature review
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Benhadid-Brahmi, Yasmine, Hamane, Samia, Soyer, Benjamin, Mebazaa, Alexandre, Alanio, Alexandre, Chousterman, Benjamin, Bretagne, Stéphane, and Dellière, Sarah
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- 2022
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18. Fungal infections in mechanically ventilated patients with COVID-19 during the first wave: the French multicentre MYCOVID study
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Gangneux, Jean-Pierre, Dannaoui, Eric, Fekkar, Arnaud, Luyt, Charles-Edouard, Botterel, Françoise, De Prost, Nicolas, Tadié, Jean-Marc, Reizine, Florian, Houzé, Sandrine, Timsit, Jean-François, Iriart, Xavier, Riu-Poulenc, Béatrice, Sendid, Boualem, Nseir, Saad, Persat, Florence, Wallet, Florent, Le Pape, Patrice, Canet, Emmanuel, Novara, Ana, Manai, Melek, Cateau, Estelle, Thille, Arnaud W, Brun, Sophie, Cohen, Yves, Alanio, Alexandre, Mégarbane, Bruno, Cornet, Muriel, Terzi, Nicolas, Lamhaut, Lionel, Sabourin, Estelle, Desoubeaux, Guillaume, Ehrmann, Stephan, Hennequin, Christophe, Voiriot, Guillaume, Nevez, Gilles, Aubron, Cécile, Letscher-Bru, Valérie, Meziani, Ferhat, Blaize, Marion, Mayaux, Julien, Monsel, Antoine, Boquel, Frédérique, Robert-Gangneux, Florence, Le Tulzo, Yves, Seguin, Philippe, Guegan, Hélène, Autier, Brice, Lesouhaitier, Matthieu, Pelletier, Romain, Belaz, Sorya, Bonnal, Christine, Berry, Antoine, Leroy, Jordan, François, Nadine, Richard, Jean-Christophe, Paulus, Sylvie, Argaud, Laurent, Dupont, Damien, Menotti, Jean, Morio, Florent, Soulié, Marie, Schwebel, Carole, Garnaud, Cécile, Guitard, Juliette, Le Gal, Solène, Quinio, Dorothée, Morcet, Jeff, Laviolle, Bruno, Zahar, Jean-Ralph, and Bougnoux, Marie-Elisabeth
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- 2022
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19. Polymerase Chain Reaction on Respiratory Tract Specimens of Immunocompromised Patients to Diagnose Pneumocystis Pneumonia: A Systematic Review and Meta-analysis
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Brown, Lottie, primary, Rautemaa-Richardson, Riina, additional, Mengoli, Carlo, additional, Alanio, Alexandre, additional, Barnes, Rosemary A, additional, Bretagne, Stéphane, additional, Chen, Sharon C A, additional, Cordonnier, Catherine, additional, Donnelly, J Peter, additional, Heinz, Werner J, additional, Jones, Brian, additional, Klingspor, Lena, additional, Loeffler, Juergen, additional, Rogers, Thomas R, additional, Rowbotham, Eleanor, additional, White, P Lewis, additional, and Cruciani, Mario, additional
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- 2024
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20. Global guideline for the diagnosis and management of cryptococcosis: an initiative of the ECMM and ISHAM in cooperation with the ASM
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MMB, MMB Medische Staf, Chang, Christina C., Harrison, Thomas S., Bicanic, Tihana A., Chayakulkeeree, Methee, Sorrell, Tania C., Warris, Adilia, Hagen, Ferry, Spec, Andrej, Oladele, Rita, Govender, Nelesh P., Chen, Sharon C., Mody, Christopher H., Groll, Andreas H., Chen, Yee Chun, Lionakis, Michail S., Alanio, Alexandre, Castañeda, Elizabeth, Lizarazo, Jairo, Vidal, José E., Takazono, Takahiro, Hoenigl, Martin, Alffenaar, Jan Willem, Gangneux, Jean Pierre, Soman, Rajeev, Zhu, Li Ping, Bonifaz, Alexandro, Jarvis, Joseph N., Day, Jeremy N., Klimko, Nikolai, Salmanton-García, Jon, Jouvion, Grégory, Meya, David B., Lawrence, David, Rahn, Sebastian, Bongomin, Felix, McMullan, Brendan J., Sprute, Rosanne, Nyazika, Tinashe K., Beardsley, Justin, Carlesse, Fabianne, Heath, Christopher H., Ayanlowo, Olusola O., Mashedi, Olga M., Queiroz-Telles Filho, Flavio, Hosseinipour, Mina C., Patel, Atul K., Temfack, Elvis, Singh, Nina, Cornely, Oliver A., Boulware, David R., Lortholary, Olivier, Pappas, Peter G., Perfect, John R., MMB, MMB Medische Staf, Chang, Christina C., Harrison, Thomas S., Bicanic, Tihana A., Chayakulkeeree, Methee, Sorrell, Tania C., Warris, Adilia, Hagen, Ferry, Spec, Andrej, Oladele, Rita, Govender, Nelesh P., Chen, Sharon C., Mody, Christopher H., Groll, Andreas H., Chen, Yee Chun, Lionakis, Michail S., Alanio, Alexandre, Castañeda, Elizabeth, Lizarazo, Jairo, Vidal, José E., Takazono, Takahiro, Hoenigl, Martin, Alffenaar, Jan Willem, Gangneux, Jean Pierre, Soman, Rajeev, Zhu, Li Ping, Bonifaz, Alexandro, Jarvis, Joseph N., Day, Jeremy N., Klimko, Nikolai, Salmanton-García, Jon, Jouvion, Grégory, Meya, David B., Lawrence, David, Rahn, Sebastian, Bongomin, Felix, McMullan, Brendan J., Sprute, Rosanne, Nyazika, Tinashe K., Beardsley, Justin, Carlesse, Fabianne, Heath, Christopher H., Ayanlowo, Olusola O., Mashedi, Olga M., Queiroz-Telles Filho, Flavio, Hosseinipour, Mina C., Patel, Atul K., Temfack, Elvis, Singh, Nina, Cornely, Oliver A., Boulware, David R., Lortholary, Olivier, Pappas, Peter G., and Perfect, John R.
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- 2024
21. WHO global research priorities for antimicrobial resistance in human health
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Bertagnolio, Silvia, Dobreva, Zlatina, Centner, Chad M, Olaru, Ioana Diana, Donà, Daniele, Burzo, Stefano, Huttner, Benedikt D, Chaillon, Antoine, Gebreselassie, Nebiat, Wi, Teodora, Hasso-Agopsowicz, Mateusz, Allegranzi, Benedetta, Sati, Hatim, Ivanovska, Verica, Kothari, Kavita U, Balkhy, Hanan H, Cassini, Alessandro, Hamers, Raph L, Weezenbeek, Kitty Van, Aanensen, David, Alanio, Alexandre, Alastruey-Izquierdo, Ana, Alemayehu, Tinsae, Al-Hasan, Majdi, Allegaert, Karel, Al-Maani, Amal Saif, Al-Salman, Jameela, Alshukairi, Abeer Nizar, Amir, Afreenish, Applegate, Tanya, Araj, George F, Villalobos, Marlen Arce, Årdal, Christine, Ashiru-Oredope, Diane, Ashley, Elizabeth A, Babin, François-Xavier, Bachmann, Laura H, Bachmann, Till, Baker, Kate Susan, Balasegaram, Manica, Bamford, Colleen, Baquero, Fernando, Barcelona, Laura Isabel, Bassat, Quique, Bassetti, Matteo, Basu, Sulagna, Beardsley, Justin, Vásquez, Grey Benoit, Berkley, James A, Bhatnagar, Anuj K, Bielicki, Julia, Bines, Julie, Bongomin, Felix, Bonomo, Robert A, Bradley, John S, Bradshaw, Catriona, Brett, Ana, Brink, Adrian, Brown, Colin, Brown, Jeremy, Buising, Kirsty, Carson, Carolee, Carvalho, Anna Cristina, Castagnola, Elio, Cavaleri, Marco, Cecchini, Michele, Chabala, Chishala, Chaisson, Richard E, Chakrabarti, Arunaloke, Chandler, Clare, Chandy, Sujith John, Charani, Esmita, Chen, Lisa, Chiara, Francesca, Chowdhary, Anuradha, Chua, Arlene, Chuki, Pem, Chun, Doo Ryeon, Churchyard, Gavin, Cirillo, Daniela, Clack, Lauren, Coffin, Susan E, Cohn, Jennifer, Cole, Michelle, Conly, John, Cooper, Ben, Corso, Alejandra, Cosgrove, Sara E, Cox, Helen, Daley, Charles L, Darboe, Saffiatou, Darton, Tom, Davies, Gerry, de Egea, Viviana, Dedeić-Ljubović, Amela, Deeves, Miranda, Denkinger, Claudia, Dillon, Jo-Anne R, Dramowski, Angela, Eley, Brian, Roberta Esposito, Susanna Maria, Essack, Sabiha Y, Farida, Helmia, Farooqi, Joveria, Feasey, Nicholas, Ferreyra, Cecilia, Fifer, Helen, Finlayson, Heather, Frick, Mike, Gales, Ana Cristina, Galli, Luisa, Gandra, Sumanth, Gerber, Jeffrey S, Giske, Christian, Gordon, Bruce, Govender, Nelesh, Guessennd, Nathalie, Guindo, Ibrehima, Gurbanova, Elmira, Gwee, Amanda, Hagen, Ferry, Harbarth, Stephan, Haze, John, Heim, Jutta, Hendriksen, Rene, Heyderman, Robert Simon, Holt, Kathryn Elizabeth, Hönigl, Martin, Hook, Edward W, Hope, William, Hopkins, Heidi, Hughes, Gwenda, Ismail, Ghada, Issack, Mohammad Iqbal, Jacobs, Jan, Jasovský, Dušan, Jehan, Fyeza, Pearson, Antonieta Jimenez, Jones, Makoto, Joshi, Mohan P, Kapil, Arti, Kariuki, Samuel, Karkey, Abhilasha, Kearns, Gregory L, Keddy, Karen Helena, Khanna, Nina, Kitamura, Akiko, Kolho, Kaija-Leena, Kontoyiannis, Dimitrios P, Kotwani, Anita, Kozlov, Roman S, Kranzer, Katharina, Kularatne, Ranmini, Lahra, Monica M, Langford, Bradley J, Laniado-Laborin, Rafael, Larsson, Joakim, Lass-Flörl, Cornelia, Le Doare, Kirsty, Lee, Hyukmin, Lessa, Fernanda, Levin, Anna S, Limmathurotsakul, Direk, Lincopan, Nilton, Lo Vecchio, Andrea, Lodha, Rakesh, Loeb, Mark, Longtin, Yves, Lye, David Chien, Mahmud, Asif Mujtaba, Manaia, Célia, Manderson, Lenore, Mareković, Ivana, Marimuthu, Kalisvar, Martin, Irene, Mashe, Tapfumanei, Mei, Zeng, Meis, Jacques F, Lyra Tavares De Melo, Flávio Augusto, Mendelson, Marc, Miranda, Angelica Espinosa, Moore, David, Morel, Chantal, Moremi, Nyambura, Moro, Maria Luisa, Moussy, Francis, Mshana, Stephen, Mueller, Arno, Ndow, Francis J, Nicol, Mark, Nunn, Andrew, Obaro, Stephen, Obiero, Christina W, Okeke, Iruka N, Okomo, Uduak, Okwor, Tochi J, Oladele, Rita, Omulo, Sylvia, Ondoa, Pascale, Ortellado de Canese, Juana Medarda, Ostrosky-Zeichner, Luis, Padoveze, Maria Clara, Pai, Madhukar, Park, Benjamin, Parkhill, Julian, Parry, Christopher M, Peeling, Rosanna, Sobreira Vieira Peixe, Luísa Maria, Perovic, Olga, Pettigrew, Melinda M, Principi, Nicola, Pulcini, Céline, Puspandari, Nelly, Rawson, Timothy, Reddy, Denasha Lavanya, Reddy, Kessendri, Redner, Paulo, Rodríguez Tudela, Juan Luis, Rodríguez-Baño, Jesús, Van Katwyk, Susan Rogers, Roilides, Emmanuel, Rollier, Christine, Rollock, Leslie, Ronat, Jean-Baptiste, Ruppe, Etienne, Sadarangani, Manish, Salisbury, David, Salou, Mounerou, Samison, Luc Hervé, Sanguinetti, Maurizio, Sartelli, Massimo, Schellack, Natalie, Schouten, Jeroen, Schwaber, Mitchell J, Seni, Jeremiah, Senok, Abiola, Shafer, William M, Shakoor, Sadia, Sheppard, Donald, Shin, Jong-Hee, Sia, Sonia, Sievert, Dawn, Singh, Ishwar, Singla, Rupak, Skov, Robert Leo, Soge, Olusegun O, Sprute, Rosanne, Srinivasan, Arjun, Srinivasan, Subasree, Sundsfjord, Arnfinn, Tacconelli, Evelina, Tahseen, Sabira, Tangcharoensathien, Viroj, Tängdén, Thomas, Thursky, Karin, Thwaites, Guy, Tigulini de Souza Peral, Renata, Tong, Deborah, Tootla, Hafsah Deepa, Tsioutis, Constantinos, Turner, Katy M, Turner, Paul, Omar, Shaheed Vally, van de Sande, Wendy WJ, van den Hof, Susan, van Doorn, Rogier, Veeraraghavan, Balaji, Verweij, Paul, Wahyuningsih, Retno, Wang, Hui, Warris, Adilia, Weinstock, Hillard, Wesangula, Evelyn, Whiley, David, White, Peter J, Williams, Phoebe, Xiao, Yonghong, Moscoso, Martin Yagui, Yang, Hsu Li, Yoshida, Sachiyo, Yu, Yunsong, Żabicka, Dorota, Zignol, Matteo, Bertagnolio, Silvia, Dobreva, Zlatina, Centner, Chad M, Olaru, Ioana Diana, Donà, Daniele, Burzo, Stefano, Huttner, Benedikt D, Chaillon, Antoine, Gebreselassie, Nebiat, Wi, Teodora, Hasso-Agopsowicz, Mateusz, Allegranzi, Benedetta, Sati, Hatim, Ivanovska, Verica, Kothari, Kavita U, Balkhy, Hanan H, Cassini, Alessandro, Hamers, Raph L, Weezenbeek, Kitty Van, Aanensen, David, Alanio, Alexandre, Alastruey-Izquierdo, Ana, Alemayehu, Tinsae, Al-Hasan, Majdi, Allegaert, Karel, Al-Maani, Amal Saif, Al-Salman, Jameela, Alshukairi, Abeer Nizar, Amir, Afreenish, Applegate, Tanya, Araj, George F, Villalobos, Marlen Arce, Årdal, Christine, Ashiru-Oredope, Diane, Ashley, Elizabeth A, Babin, François-Xavier, Bachmann, Laura H, Bachmann, Till, Baker, Kate Susan, Balasegaram, Manica, Bamford, Colleen, Baquero, Fernando, Barcelona, Laura Isabel, Bassat, Quique, Bassetti, Matteo, Basu, Sulagna, Beardsley, Justin, Vásquez, Grey Benoit, Berkley, James A, Bhatnagar, Anuj K, Bielicki, Julia, Bines, Julie, Bongomin, Felix, Bonomo, Robert A, Bradley, John S, Bradshaw, Catriona, Brett, Ana, Brink, Adrian, Brown, Colin, Brown, Jeremy, Buising, Kirsty, Carson, Carolee, Carvalho, Anna Cristina, Castagnola, Elio, Cavaleri, Marco, Cecchini, Michele, Chabala, Chishala, Chaisson, Richard E, Chakrabarti, Arunaloke, Chandler, Clare, Chandy, Sujith John, Charani, Esmita, Chen, Lisa, Chiara, Francesca, Chowdhary, Anuradha, Chua, Arlene, Chuki, Pem, Chun, Doo Ryeon, Churchyard, Gavin, Cirillo, Daniela, Clack, Lauren, Coffin, Susan E, Cohn, Jennifer, Cole, Michelle, Conly, John, Cooper, Ben, Corso, Alejandra, Cosgrove, Sara E, Cox, Helen, Daley, Charles L, Darboe, Saffiatou, Darton, Tom, Davies, Gerry, de Egea, Viviana, Dedeić-Ljubović, Amela, Deeves, Miranda, Denkinger, Claudia, Dillon, Jo-Anne R, Dramowski, Angela, Eley, Brian, Roberta Esposito, Susanna Maria, Essack, Sabiha Y, Farida, Helmia, Farooqi, Joveria, Feasey, Nicholas, Ferreyra, Cecilia, Fifer, Helen, Finlayson, Heather, Frick, Mike, Gales, Ana Cristina, Galli, Luisa, Gandra, Sumanth, Gerber, Jeffrey S, Giske, Christian, Gordon, Bruce, Govender, Nelesh, Guessennd, Nathalie, Guindo, Ibrehima, Gurbanova, Elmira, Gwee, Amanda, Hagen, Ferry, Harbarth, Stephan, Haze, John, Heim, Jutta, Hendriksen, Rene, Heyderman, Robert Simon, Holt, Kathryn Elizabeth, Hönigl, Martin, Hook, Edward W, Hope, William, Hopkins, Heidi, Hughes, Gwenda, Ismail, Ghada, Issack, Mohammad Iqbal, Jacobs, Jan, Jasovský, Dušan, Jehan, Fyeza, Pearson, Antonieta Jimenez, Jones, Makoto, Joshi, Mohan P, Kapil, Arti, Kariuki, Samuel, Karkey, Abhilasha, Kearns, Gregory L, Keddy, Karen Helena, Khanna, Nina, Kitamura, Akiko, Kolho, Kaija-Leena, Kontoyiannis, Dimitrios P, Kotwani, Anita, Kozlov, Roman S, Kranzer, Katharina, Kularatne, Ranmini, Lahra, Monica M, Langford, Bradley J, Laniado-Laborin, Rafael, Larsson, Joakim, Lass-Flörl, Cornelia, Le Doare, Kirsty, Lee, Hyukmin, Lessa, Fernanda, Levin, Anna S, Limmathurotsakul, Direk, Lincopan, Nilton, Lo Vecchio, Andrea, Lodha, Rakesh, Loeb, Mark, Longtin, Yves, Lye, David Chien, Mahmud, Asif Mujtaba, Manaia, Célia, Manderson, Lenore, Mareković, Ivana, Marimuthu, Kalisvar, Martin, Irene, Mashe, Tapfumanei, Mei, Zeng, Meis, Jacques F, Lyra Tavares De Melo, Flávio Augusto, Mendelson, Marc, Miranda, Angelica Espinosa, Moore, David, Morel, Chantal, Moremi, Nyambura, Moro, Maria Luisa, Moussy, Francis, Mshana, Stephen, Mueller, Arno, Ndow, Francis J, Nicol, Mark, Nunn, Andrew, Obaro, Stephen, Obiero, Christina W, Okeke, Iruka N, Okomo, Uduak, Okwor, Tochi J, Oladele, Rita, Omulo, Sylvia, Ondoa, Pascale, Ortellado de Canese, Juana Medarda, Ostrosky-Zeichner, Luis, Padoveze, Maria Clara, Pai, Madhukar, Park, Benjamin, Parkhill, Julian, Parry, Christopher M, Peeling, Rosanna, Sobreira Vieira Peixe, Luísa Maria, Perovic, Olga, Pettigrew, Melinda M, Principi, Nicola, Pulcini, Céline, Puspandari, Nelly, Rawson, Timothy, Reddy, Denasha Lavanya, Reddy, Kessendri, Redner, Paulo, Rodríguez Tudela, Juan Luis, Rodríguez-Baño, Jesús, Van Katwyk, Susan Rogers, Roilides, Emmanuel, Rollier, Christine, Rollock, Leslie, Ronat, Jean-Baptiste, Ruppe, Etienne, Sadarangani, Manish, Salisbury, David, Salou, Mounerou, Samison, Luc Hervé, Sanguinetti, Maurizio, Sartelli, Massimo, Schellack, Natalie, Schouten, Jeroen, Schwaber, Mitchell J, Seni, Jeremiah, Senok, Abiola, Shafer, William M, Shakoor, Sadia, Sheppard, Donald, Shin, Jong-Hee, Sia, Sonia, Sievert, Dawn, Singh, Ishwar, Singla, Rupak, Skov, Robert Leo, Soge, Olusegun O, Sprute, Rosanne, Srinivasan, Arjun, Srinivasan, Subasree, Sundsfjord, Arnfinn, Tacconelli, Evelina, Tahseen, Sabira, Tangcharoensathien, Viroj, Tängdén, Thomas, Thursky, Karin, Thwaites, Guy, Tigulini de Souza Peral, Renata, Tong, Deborah, Tootla, Hafsah Deepa, Tsioutis, Constantinos, Turner, Katy M, Turner, Paul, Omar, Shaheed Vally, van de Sande, Wendy WJ, van den Hof, Susan, van Doorn, Rogier, Veeraraghavan, Balaji, Verweij, Paul, Wahyuningsih, Retno, Wang, Hui, Warris, Adilia, Weinstock, Hillard, Wesangula, Evelyn, Whiley, David, White, Peter J, Williams, Phoebe, Xiao, Yonghong, Moscoso, Martin Yagui, Yang, Hsu Li, Yoshida, Sachiyo, Yu, Yunsong, Żabicka, Dorota, and Zignol, Matteo
- Abstract
The WHO research agenda for antimicrobial resistance (AMR) in human health has identified 40 research priorities to be addressed by the year 2030. These priorities focus on bacterial and fungal pathogens of crucial importance in addressing AMR, including drug-resistant pathogens causing tuberculosis. These research priorities encompass the entire people-centred journey, covering prevention, diagnosis, and treatment of antimicrobial-resistant infections, in addition to addressing the overarching knowledge gaps in AMR epidemiology, burden and drivers, policies and regulations, and awareness and education. The research priorities were identified through a multistage process, starting with a comprehensive scoping review of knowledge gaps, with expert inputs gathered through a survey and open call. The priority setting involved a rigorous modified Child Health and Nutrition Research Initiative approach, ensuring global representation and applicability of the findings. The ultimate goal of this research agenda is to encourage research and investment in the generation of evidence to better understand AMR dynamics and facilitate policy translation for reducing the burden and consequences of AMR.
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- 2024
22. Risk Factors and Outcome Associated With Fungal Infections in Patients With Severe Burn Injury: 10-year Retrospective IFI-BURN Study.
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Dudoignon, Emmanuel, Chevret, Sylvie, Tsague, Sorel, Hamane, Samia, Chaouat, Marc, Plaud, Benoit, Vicault, Eric, Mebazaa, Alexandre, Legrand, Matthieu, Alanio, Alexandre, Denis, Blandine, Dépret, Francois, and Dellière, Sarah
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MYCOSES ,RISK assessment ,BIOPSY ,BURNS & scalds ,RESEARCH funding ,POLYMERASE chain reaction ,SEVERITY of illness index ,RETROSPECTIVE studies ,INVASIVE candidiasis ,DESCRIPTIVE statistics ,LONGITUDINAL method ,BRONCHOALVEOLAR lavage ,CONFIDENCE intervals ,DISEASE risk factors - Abstract
Background In burn patients, skin barrier disruption and immune dysfunctions increase susceptibility to invasive fungal diseases (IFDs) like invasive candidiasis (IC) and invasive mold infections (IMI). We provide an in-depth analysis of IFD-related factors and outcomes in a 10-year cohort of severe burn patients. Methods This retrospective cohort study includes adult patients admitted to the burn intensive care unit (BICU) between April 2014 and May 2023 with total burn surface area (TBSA) ≥15%. Patients were classified as proven IFD according to EORTC/MSGERC criteria applicable for IC. Putative IMIs were defined with: ≥2 positive cultures from a skin biopsy/bronchoalveolar lavage or ≥2 positive blood specific-quantitative polymerase chain reactions (qPCRs) or a combination of both. Results Among 1381 patients admitted, 276 consecutive patients with TBSA ≥15% were included. Eighty-seven (31.5%; IC n = 30; IMI n = 43; both n = 14) patients fulfilled the criteria for probable/putative IFD. At Day 30 after the burn injury, the estimated cumulative incidence proven/putative (pr/pu) IFD was 26.4% (95% confidence interval [CI], 21.4%–31.8%). Factors independently associated with IFDs were TBSA, severity scores and indoor burn injury (ie, from confined space fire). Overall mortality was 15.3% and 36.8% in the no IFD, pr/pu IFD groups respectively (P <.0001). IFD was independently associated with a risk of death (hazard ratio [HR]: 1.94 for pr/pu IFD; 95% CI, 1.12–3.36; P =.019). Conclusions This study describes twenty-first-century characteristics of IFDs in severe burn patients confirming known risk factors with thresholds and identifying the indoor injury as an independent factor associated to IFDs. This suggests a link to contamination caused by fire damage, which is highly susceptible to aerosolizing spores. [ABSTRACT FROM AUTHOR]
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- 2024
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23. Epidemiology, Clinical Manifestations, Treatment, and Outcome of Mucormycosis: A Review of 77 Cases From a Single Center in France.
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Denis, Blandine, Resche-Rigon, Matthieu, Raffoux, Emmanuel, Ronchetti, Anne-Marie, Dudoignon, Emmanuel, Verillaud, Benjamin, Valade, Sandrine, Lorillon, Gwenaël, Rabian, Florence, Xhaard, Aliénor, Touratier, Sophie, Hamane, Samia, Alanio, Alexandre, and Castro, Nathalie De
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BLOOD diseases ,SYMPTOMS ,AMPHOTERICIN B ,MIXED infections ,POLYMERASE chain reaction ,MUCORMYCOSIS - Abstract
Background The aim of this study was to assess the epidemiology, clinical manifestations, and outcome of mucormycosis over 15 years in a single center in France. Methods We conducted a retrospective analysis of all mucormycosis cases in our institution from 1 January 2006 to 31 December 2020 and analyzed patients' medical records, laboratory results, and treatment to describe the epidemiology, clinical manifestations, diagnosis, treatment, and outcome. Mucorales quantitative polymerase chain reaction (qPCR) for the diagnosis was implemented in 2015. Results Seventy-seven mucormycosis cases were analyzed in 77 patients, with a median age of 54 years (60% male). Identified risk factors were hematological diseases (46 cases [60%]), solid malignancies (2 cases), solid organ transplants (3), burns (18), diabetes only (7), and trauma (1). Sites of infection were lungs (42%), sinus (36%), skin (31%), central nervous system (9%), liver (8%), others (6%), and disseminated (12%). Diagnosis remained difficult and qPCR contributed to mucormycosis diagnosis in 30% of cases. Among hematology patients, serum qPCR was the only positive test in 15% of cases. A mixed mold infection was diagnosed in 24 of 77 (31%) patients. Surgical treatment was undertaken in 43 (56%) cases. Most patients received liposomal amphotericin B (89%), with a combination therapy in 18 of 77 cases (23%). Three-month survival rate was 40% (95% confidence interval [CI],.30–.53]). As for treatment, adjunction of surgery (hazard ratio, 0.47 [95%CI,.25–.91); P = 0.02) was associated with lower mortality. Conclusions Mucormycosis remained associated with high mortality, especially in the hematological and burn populations. Surgery in combination with antifungal treatment was associated with improved survival. [ABSTRACT FROM AUTHOR]
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- 2024
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24. Population heterogeneity in Cryptococcus neoformans: Impact on pathogenesis.
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Agrawal, Ruchi, de Castro, Raffael J. Araújo, Sturny-Leclère, Aude, and Alanio, Alexandre
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CRYPTOCOCCUS neoformans ,MOLECULAR biology ,FUNGAL membranes ,HETEROGENEITY ,TRANSCRIPTION factors - Abstract
This article discusses the population heterogeneity in Cryptococcus neoformans and its impact on pathogenesis. Cryptococcus neoformans is a prevalent global threat, especially for people living with HIV. The article explores the different ways in which the human-Cryptococcus interaction can progress, including latent/dormant cryptococcosis, pulmonary cryptococcosis, disseminated cryptococcosis, and cryptococcal relapse. The article also examines population heterogeneity in C. neoformans, which provides benefits to the pathogen in coping with changing conditions and contributes to its survival. The specific manifestations of population heterogeneity discussed in the article include heteroresistance, viable but nonculturable state, antifungal persistence, and biofilm formation. The article highlights the influence of population heterogeneity on the survival and pathogenesis of C. neoformans. [Extracted from the article]
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- 2024
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25. Dual quantitative PCR assays for the rapid detection of Trichophyton indotineae from clinical samples.
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Baron, Audrey, Hamane, Samia, Gits-Muselli, Maud, Legendre, Lina, Benderdouche, Mazouz, Mingui, Anselme, Ghelfenstein-Ferreira, Théo, Alanio, Alexandre, and Dellière, Sarah
- Abstract
Trichophyton indotineae is an emerging species of the Trichophyton mentagrophytes complex (TMC), responsible for an epidemic of widespread hairless skin infections that is frequently (50–70%) resistant to terbinafine. In order to initiate appropriate treatment as quickly as possible without waiting for culture positivity (10–15 days) and molecular identification from the strain, we developed a dual quantitative PCR (qPCR) for the direct detection of T. indotineae in clinical samples. We first designed a T. indotineae -specific qPCR assay (TI -qPCR) targeting a single specific polymorphism in the internal transcribed spacer region. Although none of the 94 non-dermatophyte and 7 dermatophyte species were amplified, this TI -qPCR allowed amplification of other TMC species at a lower yield. With equal amounts (0.1 ng) of DNA per reaction, the mean quantitative cycle (Cq) values for T. indotineae and non- indotineae TMC were 27.9 (±0.1) and 38.9 (±0.3), respectively. Therefore, we normalized this assay against a previously validated pan-dermatophyte qPCR assay (PD-qPCR) and relied on the ΔCq [(TI -qPCR) − (PD-qPCR)] to identify T. indotineae versus other TMC species. Dual assay was validated using 86 clinical samples of culture-confirmed T. indotinea and 19 non- indotineae TMC cases. The mean ΔCq for non- indotineae TMC was 9.6 ± 2.7, whereas the ΔCq for T. indotinea was −1.46 ± 2.1 (P < .001). Setting the ΔCq at 4.5 as a cutoff value resulted in 100% specificity for the detection of T. indotineae. This dual qPCR assay quickly detects T. indotineae from skin scrapings, aiding in early diagnosis and treatment for patients with suspected infection. [ABSTRACT FROM AUTHOR]
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- 2024
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26. Revealing the hidden interplay: The unexplored relationship between fungi and viruses beyond HIV, SARS-CoV-2, and influenza
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Ghelfenstein-Ferreira, Théo, primary, Serris, Alexandra, additional, Salmona, Maud, additional, Lanternier, Fanny, additional, and Alanio, Alexandre, additional
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- 2024
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27. Features of cryptococcosis among 652 HIV-seronegative individuals in France: a cross-sectional observational study (2005-2020)
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Paccoud, Olivier, primary, Desnos-Ollivier, Marie, additional, Persat, Florence, additional, Demar, Magalie, additional, Boukris-Sitbon, Karine, additional, Bellanger, Anne-Pauline, additional, Bonhomme, Julie, additional, Bonnal, Christine, additional, Botterel, Françoise, additional, Bougnoux, Marie-Elisabeth, additional, Brun, Sophie, additional, Cassaing, Sophie, additional, Cateau, Estelle, additional, Chouaki, Taieb, additional, Cornet, Muriel, additional, Dannaoui, Eric, additional, Desbois-Nogard, Nicole, additional, Durieux, Marie-Fleur, additional, Favennec, Loïc, additional, Fekkar, Arnaud, additional, Gabriel, Frederic, additional, Gangneux, Jean-Pierre, additional, Guitard, Juliette, additional, Hasseine, Lilia, additional, Huguenin, Antoine, additional, Le Gal, Solène, additional, Letscher-Bru, Valérie, additional, Mahinc, Caroline, additional, Morio, Florent, additional, Nicolas, Muriel, additional, Poirier, Philippe, additional, Ranque, Stéphane, additional, Roosen, Gabrielle, additional, Rouges, Célia, additional, Roux, Anne-Laure, additional, Sasso, Milène, additional, Alanio, Alexandre, additional, Lortholary, Olivier, additional, Lanternier, Fanny, additional, Brieu, N., additional, Durand, C., additional, Bertei, D., additional, Bouchara, J.P., additional, Pihet, M., additional, Bland, S., additional, Bru (Annecy), J.P., additional, Pulik, M., additional, Le Turdu, F., additional, Lefrand, C, H., additional, Ferrand, M., additional, Larrouy, M., additional, Millon, L., additional, Delhaes, L., additional, Imbert, S., additional, Accoceberry, I., additional, Bachelier, M.N., additional, Nevez, G., additional, Quinio, D., additional, Le Coustumier, A., additional, Carmagnol, F., additional, Rivière, B., additional, Boex, P., additional, Podac, B., additional, Moniot, M., additional, Nourrisson, C., additional, Augereau, O., additional, Emond, J.P., additional, Belkacem-Belkaki, G., additional, Bacri, J.L., additional, Berthelot, G., additional, Dalle, F., additional, Vallee, E., additional, Bizet, J., additional, Noussair, L., additional, Herrmann, J.L., additional, Maubon, D., additional, Brocard, C., additional, Guiffault, P., additional, Layet, A., additional, Morel, A., additional, Angoulvant, A., additional, Penn, P., additional, Gigandon, A., additional, Sendid, B., additional, Cornu, M., additional, Darde, M.L., additional, Jaccard, A., additional, Bouteille, B., additional, Azjenberg, D., additional, Prades, N., additional, Bienvenu, A.L., additional, Benoit-Cattin, T., additional, Fiacre, A., additional, Levy, S., additional, Pitsch, A., additional, Kiefer, M.H., additional, Debourgogne, A., additional, Moquet, O., additional, Colot, J., additional, Courtellemont, L., additional, Poisson, D., additional, Laurens, V., additional, Kauffmann-Lacroix, C., additional, Martres, P., additional, Gargala, G., additional, Godineau, N., additional, Picot, S., additional, Chassagne, C., additional, Djibo, N., additional, Devallière, R., additional, Sabou, M., additional, Camin-Ravenne, A.M., additional, Bissuel, F., additional, Janvier, F., additional, Aubert, X., additional, Chadapaud, S., additional, Delbeck, X., additional, Lafeuillade, A., additional, Raoult, X., additional, Baclet, V., additional, Coignard, C., additional, Mouton, Y., additional, Ravaux, I., additional, Eloy, C., additional, Fur, A., additional, Rezzouk, L., additional, Mazards, E., additional, Eloy, O., additional, Chachaty, E., additional, Mihaila, L., additional, Dellion, S., additional, Patey, O., additional, Thouvenot, A., additional, Limousin, L., additional, Paugam, A., additional, Desplaces, N., additional, Raguin, G., additional, Sitterlé, E., additional, Blaize, M., additional, Gits-Muselli, M., additional, Hennequin, C., additional, Poirot, J.L., additional, Bretagne, S., additional, Lacroix, Claire, additional, and Hamane, Samia, additional
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- 2024
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28. Development and validation of quantitative PCR assays for HIV-associated cryptococcal meningitis in sub-Saharan Africa: a diagnostic accuracy study
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Mbangiwa, Tshepiso, primary, Sturny-Leclère, Aude, additional, Lechiile, Kwana, additional, Kajanga, Cheusisime, additional, Boyer-Chammard, Timothée, additional, Hoving, Jennifer C, additional, Leeme, Tshepo, additional, Moyo, Melanie, additional, Youssouf, Nabila, additional, Lawrence, David S, additional, Mwandumba, Henry, additional, Mosepele, Mosepele, additional, Harrison, Thomas S, additional, Jarvis, Joseph N, additional, Lortholary, Olivier, additional, Alanio, Alexandre, additional, Goodall, J, additional, Mawoko, N, additional, Milburn, J, additional, Mmipi, R, additional, Muthoga, C, additional, Ponatshego, P, additional, Rulaganyang, I, additional, Seatla, K, additional, Tlhako, N, additional, Tsholo, K, additional, April, S, additional, Bekiswa, A, additional, Boloko, L, additional, Bookholane, H, additional, Crede, T, additional, Davids, L, additional, Goliath, R, additional, Hlungulu, S, additional, Hoffman, R, additional, Kyepa, H, additional, Masina, N, additional, Maughan, D, additional, Mnguni, T, additional, Moosa, S, additional, Morar, T, additional, Mpalali, M, additional, Naude, J, additional, Oliphant, I, additional, Sayed, S, additional, Sebesho, L, additional, Shey, M, additional, Swanepoel, L, additional, Chasweka, M, additional, Chimang’anga, W, additional, Chimphambano, T, additional, Dziwani, E, additional, Gondwe, E, additional, Kadzilimbile, A, additional, Kateta, S, additional, Kossam, E, additional, Kukacha, C, additional, Lipenga, B, additional, Ndaferankhande, J, additional, Ndalama, M, additional, Shah, R, additional, Singini, A, additional, Stott, K, additional, Zambasa, A, additional, Banda, T, additional, Chikaonda, T, additional, Chitulo, G, additional, Chiwoko, L, additional, Chome, N, additional, Gwin, M, additional, Kachitosi, T, additional, Kamanga, B, additional, Kazembe, M, additional, Kumwenda, E, additional, Kumwenda, M, additional, Maya, C, additional, Mhango, W, additional, Mphande, C, additional, Msumba, L, additional, Munthali, T, additional, Ngoma, D, additional, Nicholas, S, additional, Simwinga, L, additional, Stambuli, A, additional, Tegha, G, additional, Zambezi, J, additional, Ahimbisibwe, C, additional, Akampurira, A, additional, Alice, A, additional, Cresswell, F, additional, Gakuru, J, additional, Kiiza, D, additional, Kisembo, J, additional, Kwizera, R, additional, Kugonza, F, additional, Laker, E, additional, Luggya, T, additional, Lule, A, additional, Musubire, A, additional, Muyise, R, additional, Namujju, O, additional, Ndyetukira, J, additional, Nsangi, L, additional, Okirwoth, M, additional, Sadiq, A, additional, Tadeo, K, additional, Tukundane, A, additional, Williams, D, additional, Atwine, L, additional, Buzaare, P, additional, Collins, M, additional, Emily, N, additional, Inyakuwa, C, additional, Kariisa, S, additional, Mwesigye, J, additional, Niwamanya, S, additional, Rodgers, A, additional, Rukundo, J, additional, Rwomushana, I, additional, Ssemusu, M, additional, Stead, G, additional, Boyd, K, additional, Gondo, S, additional, Kufa, P, additional, Makaha, E, additional, Moyo, C, additional, Mtisi, T, additional, Mudzingwa, S, additional, Mwarumba, T, additional, Zinyandu, T, additional, Dromer, F, additional, Johnstone, P, additional, and Hafeez, S, additional
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- 2024
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29. Cladophialophora Bantiana Phaeohyphomycosis in France: A Reappraisal of Epidemiology, Therapeutic Strategy, and Outcome
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Lortholary, Olivier, primary, Garcia-Hermoso, Dea, additional, Sturny Leclère, Aude, additional, Boukris-Sitbon, Karine, additional, Nourrisson, Celine, additional, Letscher-Bru, Valérie, additional, Desbois-Nogard, Nicole, additional, Bani-Sadr, Firouzé, additional, Bienvenu, Boris, additional, Cordier, Camille, additional, Coste, Anne, additional, Danion, François, additional, Degot, Tristan, additional, Delarbre, David, additional, Fekkar, Arnaud, additional, Garcie, Christophe, additional, Garrouste, Cyril, additional, Desoubeaux, Guillaume, additional, Gits-Muselli, Maud, additional, Guemas, Emilie, additional, Hugenin, Antoine, additional, Janvier, Frédéric, additional, Kamar, NAssim, additional, Kervinio, Cyril., additional, Le Gal, Solène, additional, Lesens, Olivier, additional, Machouart, Marie, additional, Persat, Florence, additional, Picot, Sandrine, additional, Rouze, Anahita, additional, Ranque, Stéphane, additional, Ruch, Yvon, additional, Saada, Matthieu, additional, Stabler, Sarah, additional, Alanio, Alexandre, additional, Lanternier, Fanny, additional, and Bastides, Frederic, additional
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- 2024
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30. Gradient concentration strip–specific epidemiological cut-off values of antifungal drugs in various yeast species and five prevalent Aspergillus species complexes
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Mercier, Victor, Letscher-Bru, Valérie, Bougnoux, Marie-Elisabeth, Delhaes, Laurence, Botterel, Francoise, Maubon, Danièle, Dalle, Frédéric, Alanio, Alexandre, Houzé, Sandrine, Dannaoui, Eric, Cassagne, Carole, Cassaing, Sophie, Durieux, Marie-Fleur, Fekkar, Arnaud, Bouchara, Jean-Philippe, Gangneux, Jean-Pierre, Bonhomme, Julie, Dupont, Damien, Costa, Damien, Sendid, Boualem, Chouaki, Taieb, Bourgeois, Nathalie, Huguenin, Antoine, Brun, Sophie, Mahinc, Caroline, Hasseine, Lilia, Le Gal, Solène, Bellanger, Anne-Pauline, Bailly, Eric, Morio, Florent, Nourrisson, Céline, Desbois-Nogard, Nicole, Perraud-Cateau, Estelle, Debourgogne, Anne, Yéra, Hélène, Lachaud, Laurence, and Sasso, Milène
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- 2023
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31. Imported leishmaniasis in travelers: a 7-year retrospective from a Parisian hospital in France
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Aissaoui, Nesrine, Hamane, Samia, Gits-Muselli, Maud, Petit, Antoine, Benderdouche, Mazouz, Denis, Blandine, Alanio, Alexandre, Dellière, Sarah, Bagot, Martine, and Bretagne, Stéphane
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- 2021
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32. Kicking sleepers out of bed: Macrophages promote reactivation of dormant Cryptococcus neoformans by extracellular vesicle release and non-lytic exocytosis
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de Castro, Raffael Júnio Araújo, primary, Marina, Clara Luna, additional, Sturny-Leclère, Aude, additional, Hoffmann, Christian, additional, Bürgel, Pedro Henrique, additional, Wong, Sarah Sze Wah, additional, Aimanianda, Vishukumar, additional, Varet, Hugo, additional, Agrawal, Ruchi, additional, Bocca, Anamélia Lorenzetti, additional, and Alanio, Alexandre, additional
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- 2023
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33. Bronchial aspirate obtained during bronchoscopy yields increased fungal load compared to bronchoalveolar lavage fluid in patients at risk of invasive aspergillosis and Pneumocystis pneumonia
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Dellière, Sarah, primary, Amar, Yaël, additional, Hamane, Samia, additional, Aissaoui, Nesrine, additional, Denis, Blandine, additional, Bergeron, Anne, additional, Tazi, Abdellatif, additional, and Alanio, Alexandre, additional
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- 2023
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34. Multiple colony antifungal susceptibility testing detects polyresistance in clinical Candida cultures: a European Confederation of Medical Mycology excellence centers study
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Knoll, Miriam A., Lackner, Nina, Ulmer, Hanno, Samardzic, Eldina, Steinmann, Joerg, Krause, Robert, Verhasselt, Hedda L., Rath, Peter-Michael, Fuchs, Frieder, Koehler, Philipp, Denis, Blandine, Hamane, Samia, Alanio, Alexandre, and Lass-Flörl, Cornelia
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- 2022
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35. Invasive fungal diseases in patients with autoimmune diseases: a case series from the French RESSIF network
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Galmiche, Simon, primary, Thoreau, Benjamin, additional, Bretagne, Stéphane, additional, Alanio, Alexandre, additional, Paugam, André, additional, Letscher-Bru, Valérie, additional, Cassaing, Sophie, additional, Gangneux, Jean-Pierre, additional, Guegan, Hélène, additional, Favennec, Loïc, additional, Minoza, Alida, additional, Morio, Florent, additional, Bonhomme, Julie, additional, Desoubeaux, Guillaume, additional, Eloy, Odile, additional, Hasseine, Lilia, additional, Sasso, Milène, additional, Millon, Laurence, additional, Bellanger, Anne-Pauline, additional, Poirier, Philippe, additional, Moniot, Maxime, additional, Chouaki, Taieb, additional, Huguenin, Antoine, additional, Dalle, Frédéric, additional, Bouteille, Bernard, additional, Nicolas, Muriel, additional, Desbois-Nogard, Nicole, additional, Bougnoux, Marie-Elisabeth, additional, Danion, François, additional, Poindron, Vincent, additional, Néel, Antoine, additional, Boukris-Sitbon, Karine, additional, Lanternier, Fanny, additional, and Terrier, Benjamin, additional
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- 2023
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36. Development and validation of a new quantitative reverse transcription PCR assay for the diagnosis of human sporotrichosis
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Marques de Macedo, Priscila, primary, Sturny-Leclère, Aude, additional, Freitas, Dayvison Francis Saraiva, additional, Ghelfenstein-Ferreira, Theo, additional, Gutierrez-Galhardo, Maria Clara, additional, Almeida, Marcos de Abreu, additional, Rodrigues, Anderson Messias, additional, Pautet, Thierry, additional, Hamane, Samia, additional, Almeida-Paes, Rodrigo, additional, Zancopé-Oliveira, Rosely Maria, additional, and Alanio, Alexandre, additional
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- 2023
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37. Cryptococcus neoformans Infections Differ Among Human Immunodeficiency Virus (HIV)–Seropositive and HIV-Seronegative Individuals: Results From a Nationwide Surveillance Program in France.
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Paccoud, Olivier, Desnos-Ollivier, Marie, Cassaing, Sophie, Boukris-Sitbon, Karine, Alanio, Alexandre, Bellanger, Anne-Pauline, Bonnal, Christine, Bonhomme, Julie, Botterel, Françoise, Bougnoux, Marie-Elisabeth, Brun, Sophie, Chouaki, Taieb, Cornet, Muriel, Dannaoui, Eric, Demar, Magalie, Desbois-Nogard, Nicole, Durieux, Marie-Fleur, Favennec, Loïc, Fekkar, Arnaud, and Gabriel, Frederic
- Subjects
HIV ,CRYPTOCOCCUS neoformans ,SYMPTOMS ,CRYPTOCOCCOSIS ,HIV status - Abstract
Among 1107 cryptococcosis cases from the French surveillance network (2005–2020), the proportion of HIV-seronegative individuals has recently surpassed that of HIV-seropositive individuals. We observed marked differences in patient characteristics, disease presentations, cryptococcal antigen results, infecting species, and mortality according to HIV serostatus. [ABSTRACT FROM AUTHOR]
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- 2024
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38. Coregulation of extracellular vesicle production and fluconazole susceptibility in Cryptococcus neoformans
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Rizzo, Juliana, primary, Trottier, Adèle, additional, Moyrand, Frédérique, additional, Coppée, Jean-Yves, additional, Maufrais, Corinne, additional, Zimbres, Ana Claudia G., additional, Dang, Thi Tuong Vi, additional, Alanio, Alexandre, additional, Desnos-Ollivier, Marie, additional, Mouyna, Isabelle, additional, Péhau-Arnaude, Gérard, additional, Commere, Pierre-Henri, additional, Novault, Sophie, additional, Ene, Iuliana V., additional, Nimrichter, Leonardo, additional, Rodrigues, Marcio L., additional, and Janbon, Guilhem, additional
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- 2023
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39. A multicentre external quality assessment: A first step to standardise PCR protocols for the diagnosis of histoplasmosis and coccidioidomycosis
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Wilmes, Dunja, primary, Hagen, Ferry, additional, Verissimo, Cristina, additional, Alanio, Alexandre, additional, Rickerts, Volker, additional, and Buitrago, Maria José, additional
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- 2023
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40. A multicentre external quality assessment: A first step to standardise PCR protocols for the diagnosis of histoplasmosis and coccidioidomycosis
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Wilmes, Dunja, Hagen, Ferry, Verissimo, Cristina, Alanio, Alexandre, Rickerts, Volker, Buitrago, Maria José, Wilmes, Dunja, Hagen, Ferry, Verissimo, Cristina, Alanio, Alexandre, Rickerts, Volker, and Buitrago, Maria José
- Abstract
BACKGROUND: In-house real-time PCR (qPCR) is increasingly used to diagnose the so-called endemic mycoses as commercial assays are not widely available.OBJECTIVES: To compare the performance of different molecular diagnostic assays for detecting Histoplasma capsulatum and Coccidioides spp. in five European reference laboratories.METHODS: Two blinded external quality assessment (EQA) panels were sent to each laboratory that performed the analysis with their in-house assays. Both panels included a range of concentrations of H. capsulatum (n = 7) and Coccidioides spp. (n = 6), negative control and DNA from other fungi. Four laboratories used specific qPCRs, and one laboratory a broad-range fungal conventional PCR (cPCR) and a specific cPCR for H. capsulatum with subsequent sequencing.RESULTS: qPCR assays were the most sensitive for the detection of H. capsulatum DNA. The lowest amount of H. capsulatum DNA detected was 1-4 fg, 0.1 pg and 10 pg for qPCRs, specific cPCR and broad-range cPCR, respectively. False positive results occurred with high concentrations of Blastomyces dermatitidis DNA in two laboratories and with Emergomyces spp. in one laboratory. For the Coccidioides panel, the lowest amount of DNA detected was 1-16 fg by qPCRs and 10 pg with the broad-range cPCR. One laboratory reported a false positive result by qPCR with high load of Uncinocarpus DNA.CONCLUSION: All five laboratories were able to correctly detect H. capsulatum and Coccidioides spp. DNA and qPCRs had a better performance than specific cPCR and broad-range cPCR. EQAs may help standardise in-house molecular tests for the so-called endemic mycoses improving patient management.
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- 2023
41. Multicenter Evaluation of the FilmArray Blood Culture Identification 2 Panel for Pathogen Detection in Bloodstream Infections
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Caméléna, François, primary, Péan de Ponfilly, Gauthier, additional, Pailhoriès, Hélène, additional, Bonzon, Lucas, additional, Alanio, Alexandre, additional, Poncin, Thibaut, additional, Lafaurie, Matthieu, additional, Dépret, François, additional, Cambau, Emmanuel, additional, Godreuil, Sylvain, additional, Chenouard, Rachel, additional, Le Monnier, Alban, additional, Jacquier, Hervé, additional, and Berçot, Béatrice, additional
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- 2023
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42. Coregulation of extracellular vesicle production and fluconazole susceptibility inCryptococcus neoformans
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Rizzo, Juliana, primary, Trottier, Adèle, additional, Moyrand, Frédérique, additional, Coppee, Jean-Yves, additional, Maufrais, Corinne, additional, Zimbres, Ana Claudia G., additional, Dang, Thi Tuong Vi, additional, Alanio, Alexandre, additional, Desnos-Ollivier, Marie, additional, Mouyna, Isabelle, additional, Péhau-Arnaudet, Gérard, additional, Commere, Pierre-Henri, additional, Novault, Sophie, additional, Ene, Iuliana V., additional, Nimrichter, Leonardo, additional, Rodrigues, Marcio L., additional, and Janbon, Guilhem, additional
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- 2023
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43. PCR on Respiratory Tract Specimens of Immunocompromised Patients to Diagnose Pneumocystosis (PcP): A Systematic Review and Meta-Analysis
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Brown, Lottie, primary, Rautemaa-Richardson, Riina, additional, Mengoli, Carlo, additional, Alanio, Alexandre, additional, Barnes, Rosemary A., additional, Bretagne, Stephane, additional, Chen, Sharon C-A., additional, Cordonnier, Catherine, additional, Donnelly, Peter J., additional, Heinz, Werner J., additional, Jones, Brian, additional, Klingspor, Lena, additional, Loeffler, Juergen, additional, Rogers, Thomas R., additional, Rowbotham, Eleanor, additional, White, Lewis, additional, and Cruciani, Mario, additional
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- 2023
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44. Cost-effectiveness of single, high-dose, liposomal amphotericin regimen for HIV-associated cryptococcal meningitis in five countries in sub-Saharan Africa: an economic analysis of the AMBITION-cm trial
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Lawrence, David S, primary, Muthoga, Charles, additional, Meya, David B, additional, Tugume, Lillian, additional, Williams, Darlisha, additional, Rajasingham, Radha, additional, Boulware, David R, additional, Mwandumba, Henry C, additional, Moyo, Melanie, additional, Dziwani, Eltas N, additional, Maheswaran, Hendramoorthy, additional, Kanyama, Cecilia, additional, Hosseinipour, Mina C, additional, Chawinga, Chimwemwe, additional, Meintjes, Graeme, additional, Schutz, Charlotte, additional, Comins, Kyla, additional, Bango, Funeka, additional, Muzoora, Conrad, additional, Jjunju, Samuel, additional, Nuwagira, Edwin, additional, Mosepele, Mosepele, additional, Leeme, Tshepo, additional, Ndhlovu, Chiratidzo E, additional, Hlupeni, Admire, additional, Shamu, Shepherd, additional, Boyer-Chammard, Timothée, additional, Molloy, Síle F, additional, Youssouf, Nabila, additional, Chen, Tao, additional, Shiri, Tinevimbo, additional, Jaffar, Shabbar, additional, Harrison, Thomas S, additional, Jarvis, Joseph N, additional, Niessen, Louis W, additional, Goodall, Jack, additional, Lechiile, Kwana, additional, Mawoko, Norah, additional, Mbangiwa, Tshepiso, additional, Milburn, James, additional, Mmipi, Refilwe, additional, Ponatshego, Ponego, additional, Rulaganyang, Ikanyang, additional, Seatla, Kaelo, additional, Siamisang, Keatlaretse, additional, Tlhako, Nametso, additional, Tsholo, Katlego, additional, April, Samantha, additional, Bekiswa, Abulele, additional, Boloko, Linda, additional, Bookholane, Hloni, additional, Crede, Thomas, additional, Davids, Lee-Ann, additional, Goliath, Rene, additional, Hlungulu, Siphokazi, additional, Hoffman, Regina, additional, Kyepa, Henriette, additional, Masina, Noma, additional, Maughan, Deborah, additional, Mnguni, Trevor, additional, Moosa, Sumaiyya, additional, Morar, Tania, additional, Mpalali, Mkanyiseli, additional, Naude, Jonathan, additional, Oliphant, Ida, additional, Singh, Achita, additional, Sayed, Sumaya, additional, Sebesho, Leago, additional, Shey, Muki, additional, Swanepoel, Loraine, additional, Chasweka, Madalitso, additional, Chimang'anga, Wezi, additional, Chimphambano, Tipatseni, additional, Gondwe, Ebbie, additional, Mzinganjira, Henry, additional, Kadzilimbile, Aubrey, additional, Kateta, Steven, additional, Kossam, Evelyn, additional, Kukacha, Christopher, additional, Lipenga, Bright, additional, Ndaferankhande, John, additional, Ndalama, Maureen, additional, Shah, Reya, additional, Singini, Andreas, additional, Stott, Katherine, additional, Zambasa, Agness, additional, Banda, Towera, additional, Chikaonda, Tarsizio, additional, Chitulo, Gladys, additional, Chiwoko, Lorren, additional, Chome, Nelecy, additional, Gwin, Mary, additional, Kachitosi, Timothy, additional, Kamanga, Beauty, additional, Kazembe, Mussah, additional, Kumwenda, Emily, additional, Kumwenda, Masida, additional, Maya, Chimwemwe, additional, Mhango, Wilberforce, additional, Mphande, Chimwemwe, additional, Msumba, Lusungu, additional, Munthali, Tapiwa, additional, Ngoma, Doris, additional, Nicholas, Simon, additional, Simwinga, Lusayo, additional, Stambuli, Anthony, additional, Tegha, Gerald, additional, Zambezi, Janet, additional, Ahimbisibwe, Cynthia, additional, Akampurira, Andrew, additional, Alice, Anamudde, additional, Cresswell, Fiona, additional, Gakuru, Jane, additional, Kagimu, Enock, additional, Kasibante, John, additional, Kiiza, Daniel, additional, Kisembo, John, additional, Kwizera, Richard, additional, Kugonza, Florence, additional, Laker, Eva, additional, Luggya, Tonny, additional, Lule, Andrew, additional, Musubire, Abdu, additional, Muyise, Rhona, additional, Namujju, Carol Olivie, additional, Ndyetukira, Jane Francis, additional, Nsangi, Laura, additional, Okirworth, Michael, additional, Rhein, Joshua, additional, Rutakingirwa, Morris K, additional, Sadiq, Alisat, additional, Ssebambulidde, Kenneth, additional, Tadeo, Kiiza, additional, Tukundane, Asmus, additional, Atwine, Leo, additional, Buzaare, Peter, additional, Collins, Muganzi, additional, Emily, Ninsima, additional, Inyakuwa, Christine, additional, Kariisa, Samson, additional, Mwesigye, James, additional, Nuwamanya, Simpson, additional, Rodgers, Ankunda, additional, Rukundo, Joan, additional, Rwomushana, Irene, additional, Ssemusu, Mike, additional, Stead, Gavin, additional, Boyd, Kathyrn, additional, Gondo, Secrecy, additional, Kufa, Prosper, additional, Makaha, Edward, additional, Moyo, Colombus, additional, Mtisi, Takudzwa, additional, Mudzinga, Shepherd, additional, Mutata, Constantine, additional, Mwarumba, Taddy, additional, Zinyandu, Tawanda, additional, Alanio, Alexandre, additional, Dromer, Francoise, additional, Lortholary, Olivier, additional, Sturny-Leclere, Aude, additional, Griffin, Philippa, additional, Hafeez, Sophia, additional, Loyse, Angela, additional, and van Widenfelt, Erik, additional
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- 2022
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45. Engineered Fluorescent Strains of Cryptococcus neoformans: a Versatile Toolbox for Studies of Host-Pathogen Interactions and Fungal Biology, Including the Viable but Nonculturable State
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de Castro, Raffael Júnio Araújo, primary, Rêgo, Marco Túlio Aidar Mariano, additional, Brandão, Fabiana S., additional, Pérez, Ana Laura Alfonso, additional, De Marco, Janice Lisboa, additional, Poças-Fonseca, Marcio José, additional, Nichols, Connie, additional, Alspaugh, J. Andrew, additional, Felipe, Maria Sueli S., additional, Alanio, Alexandre, additional, Bocca, Anamélia Lorenzetti, additional, and Fernandes, Larissa, additional
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- 2022
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46. First Patient-to-Patient Intrahospital Transmission of Clade I Candida auris in France Revealed after a Two-Month Incubation Period
- Author
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Alanio, Alexandre, primary, Snell, Hannah Marie, additional, Cordier, Camille, additional, Desnos-Olivier, Marie, additional, Dellière, Sarah, additional, Aissaoui, Nesrine, additional, Sturny-Leclère, Aude, additional, Da Silva, Elodie, additional, Eblé, Cyril, additional, Rouveau, Martine, additional, Thégat, Micheline, additional, Zebiche, Widad, additional, Lafaurie, Matthieu, additional, Denis, Blandine, additional, Touratier, Sophie, additional, Benyamina, Mourad, additional, Dudoignon, Emmanuel, additional, Hamane, Samia, additional, Cuomo, Christina A., additional, and Dépret, François, additional
- Published
- 2022
- Full Text
- View/download PDF
47. Performance of the beta-glucan test for the diagnosis of invasive fusariosis and scedosporiosis: a meta-analysis.
- Author
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Lamoth, Frederic, Nucci, Marcio, Fernandez-Cruz, Ana, Azoulay, Elie, Lanternier, Fanny, Bremerich, Jens, Einsele, Hermann, Johnson, Elizabeth, Lehrnbecher, Thomas, Mercier, Toine, Porto, Luciana, Verweij, Paul E, White, Lewis, Maertens, Johan, Alanio, Alexandre, and (ECIL-9), the 9th European Conference on Infections in Leukemia
- Abstract
The (1→3)-β-D-glucan (BDG) is a component of the fungal cell wall that can be detected in serum and used as an adjunctive tool for the diagnosis of invasive mold infections (IMI) in patients with hematologic cancer or other immunosuppressive conditions. However, its use is limited by modest sensitivity/specificity, inability to differentiate between fungal pathogens, and lack of detection of mucormycosis. Data about BDG performance for other relevant IMI, such as invasive fusariosis (IF) and invasive scedosporiosis/lomentosporiosis (IS) are scarce. The objective of this study was to assess the sensitivity of BDG for the diagnosis of IF and IS through systematic literature review and meta-analysis. Immunosuppressed patients diagnosed with proven or probable IF and IS, with interpretable BDG data were eligible. A total of 73 IF and 27 IS cases were included. The sensitivity of BDG for IF and IS diagnosis was 76.7% and 81.5%, respectively. In comparison, the sensitivity of serum galactomannan for IF was 27%. Importantly, BDG positivity preceded the diagnosis by conventional methods (culture or histopathology) in 73% and 94% of IF and IS cases, respectively. Specificity was not assessed because of lacking data. In conclusion, BDG testing may be useful in patients with suspected IF or IS. Combining BDG and galactomannan testing may also help differentiating between the different types of IMI. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
48. Investigation of CryptoPS LFA-positive sera in patients at risk of cryptococcosis
- Author
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Aissaoui, Nesrine, primary, Benhadid-Brahmi, Yasmine, additional, Sturny-Leclère, Aude, additional, Hamane, Samia, additional, Payet, Eliane, additional, Bonnal, Christine, additional, Munier, Anne-Lise, additional, Denis, Blandine, additional, and Alanio, Alexandre, additional
- Published
- 2022
- Full Text
- View/download PDF
49. S1.4d Cryptococcus qPCR assays: the future for routine mycology labs and clinical trials dealing with cryptococcosis
- Author
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Mbangiwa, Tshepiso, primary, Sturny-Leclère, Aude, additional, Lechiile, Kwana, additional, Kajanga, Cheusisime, additional, Chammard, Timothée Boyer, additional, Lortholary, Olivier, additional, Dromer, Francoise Dromer, additional, Hoving, Jennifer C., additional, Lawrence, David S., additional, Mwandumba, Henry, additional, Mosepele, Mosepele, additional, Harrison, Tom, additional, Jarvis, Joseph N, additional, and Alanio, Alexandre, additional
- Published
- 2022
- Full Text
- View/download PDF
50. P381 Kinetics of Titan cells generation and transcriptome modifications comparing three in vitro protocols
- Author
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Leclere, Aude Sturny, primary, Lemee, Laure, additional, Cokelaer, Thomas, additional, and Alanio, Alexandre, additional
- Published
- 2022
- Full Text
- View/download PDF
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