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This study was performed to evaluate the effect of different decontamination protocols applied to contaminated surfaces on the recovery of adhesion. Different decontamination protocols were applied to molar teeth contaminated with blood and saliva except the control group. 40 third molar teeth were divided into groups according to the applied decontamination protocols: Group 1(control), Group 2 (water rinse), Group 3 (15-sec re-etch), Group 4 (10-sec re-etch), Group 5 (5-sec re-etch), Group 6 (5% NaOCl), Group 7 (2% chlorhexidine gluconate), Group 8 (ozonated water). Universal adhesive resin (Gluma Universal, Heraus Kulzer) was used in the etch and rinse mode according to the manufacturer's instructions. Nanohybrid composite material was placed in all groups. Then the Shear Bond strengths of all groups were evaluated after applying 5000 thermal cycles (5–55 °C) to each sample. In the limitation of this in vitro study, it has been shown that rinsing with ozonated water of contaminated surfaces with blood and saliva after etching, has increased the micro-shear bond strength (μSBS) values more than that of in control group values. Also, the application of NaOCl, re-etching with 37% phosphoric acid for 15 seconds, and cleaning with CHX, significantly increase the bonding potential. [ABSTRACT FROM AUTHOR]