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39 results on '"Abe, Tomoko"'

17. ポストたんぽぽ計画での宇宙曝露実験の進捗情報報告2022

Author

T., Milojevic, CHANG-HYUN, Maeng, MITA, Hajime, YANO, Hajime, TOMITA-YOKOTANI, Kaori, SUGIMOTO, Manabu, FUJITA, Tomomichi, YOKOBORI, Shinichi, BESSHO, Yoshitaka, NAKAGAWA, Kazumichi, KOBAYASHI, Kensei, KEBUKAWA, Yoko, YAMAGISHI, Akihiko, KATOH, Hiroshi, ABE, Tomoko, KIMURA, Shunta, ONG, Midori, SUZUKI, Toshisada, ASANO, Maki, HIWATARI, Yuji, KUME, Atsushi, OKUDAIRA, Kyoko, and Tanpopo, Team

Abstract

第37回宇宙環境利用シンポジウム (2023年1月17日-18日. オンライン開催), Space Utilization Research (January 17-18, 2023. Online Meeting), 著者人数: 22名ほか, 資料番号: SA6000180007, G-1

Published
2023

18. Robust Mutants Isolated through Heavy-Ion Beam Irradiation and Endurance Screening in the Green Alga Haematococcus pluvialis

Author

Takeshita, Tsuyoshi, Takita, Kaori, Kotaro, Ishii, Kazama, Yusuke, Abe, Tomoko, and Kawano, Shigeyuki

Abstract

Heavy-ion beams have been widely utilized as a novel and effective mutagen for mutation breeding in diverse species, including algae, but a preferred mutant cannot be easily obtained without a suitable large-scale screening method. We devised a unique, convenient, and effective method for screening mutants of Haematococcus pluvialis to isolate a strain resistant to environmental stress with low white fluorescence, i.e., a robust strain. Haematococcus was irradiated with heavy-ion beams of carbon ions, argon ions, and iron ions at various doses, after which approximately 10,000 surviving colonies were inoculated into 96-well plates, cultured for approximately 2 weeks, and then left to dry in a refrigerator for 3–12 months without a lid. In these unattended 96-well plates, cells in approximately one-third of the wells died and became white, and the remaining wells were approximately evenly split between red and green. The robustness of wild-type and mutant strains isolated from red and green wells was compared under severe environmental-stress conditions (125 µmol photons m−2 s−1, continuous light period, 45 mM sodium acetate). In the wild-type strain, most cells died, and 93.9% of cells emitted white autofluorescence. In contrast, few G4 cells emitted white autofluorescence, indicating a survival rate of 91.8%. Strains with excellent carotenoid production, such as G7 and R1, showed greater robustness compared to wild-type strains.

Published
2021

19. Male/Female Trade-Off in Hermaphroditic Y-Chromosome Deletion Mutants of the Dioecious Plant Silene latifolia

Author

Aonuma, Wataru, Kawamoto, Hiroki, Kazama, Yusuke, Ishii, Kotaro, Abe, Tomoko, Kawano, Shigeyuki, and Kotaro, Ishii

Abstract

The evolution of dioecy from hermaphroditism allows for avoidance of self-pollination, and its evolutionary background has been investigated both experimentally and theoretically since it was first proposed by Darwin. To reproduce this evolution, we screened hermaphroditic mutants of Silene latifolia using heavy-ion beam or γ-ray irradiation and characterized the phenotypes of their floral organs. Our scatterplots indicate severe deviations from the trade-off relationships between pollen and ovule numbers and between seed and germinated pollen numbers in hermaphroditic mutant S. latifolia. These deviations presumably led to promotion of dioecy from the ancestral state of S. latifolia. To infer the likely flower phenotypic characteristics of the ancestral plant of S. latifolia before evolving dioecy, the flowers of Silene viscosa, a naturally hermaphroditic plant related to S. latifolia were also characterized. S. viscosa exhibits both spatial separation of stamens from pistils within the flower (reverse herkogamy) and temporal separation of stamen and pistil maturation (dichogamy), raising the question of whether hermaphroditic mutant S. latifolia, which is thought to be the ancestral state, would possess these functions. We show that two hermaphroditic mutants of the dioecious plant S. latifolia exhibit signs of protogyny (reverse dichogamy) and approach herkogamy, as pistils were constantly longer than stamens. These findings illustrate the evolution of dioecy from hermaphroditism as a self-pollination avoidance mechanism and to balance the investments into male and female functions.

Published
2021

20. Effects of dietary microalgal species and hormone treatment on the lorica size and reproductivity of heavy-ion beam irradiated rotifers

Author

Kim, Hee-Jin, primary, Nakamura, Kaichi, additional, Sakakura, Yoshitaka, additional, Suga, Koushirou, additional, Tsuneizumi, Kazuhide, additional, Abe, Tomoko, additional, Yamada, Mieko, additional, Kawada, Miki, additional, Katayama, Takashi, additional, Tezuka, Nobuhiro, additional, Kobayashi, Takanori, additional, Koiso, Masahiko, additional, and Hagiwara, Atsushi, additional

Published
2022
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26. Space Experiments in cyanobacteria on Tanpopo 1 and 2 mission

Author

たんぽぽ1, 2メンバ, TOMITA-YOKOTANI, Kaori, KIMURA, Shunta, ONG, Midori, TOKITA, Miku, KATOH, Hiroshi, ABE, Tomoko, HASHIMOTO, Hirofumi, SONOIKE, Kintake, OHMORI, Masayuki, YAMAGICHI, Akihiko, and MITA, Hajime

Subjects
International space station, Nostoc sp. HK-01. Space exposure experiment, Tanpopo mission, Cyanobacteria
Abstract

第36回宇宙環境利用シンポジウム (2022年1月18日-19日. オンライン開催), Space Utilization Research (January 18-19, 2022. Online Meeting), 著者人数: 11名ほか, 資料番号: SA6000168003, G-02

Published
2022

27. ポストたんぽぽ計画の進捗状況

Author

MILOJEVIC, Tetyana, MITA, Hajime, YANO, Hajime, SAKON, Itsuki, KOBAYASHI, Kensei, KEBUKAWA, Yoko, TOMITA-YOKOTANI, Kaori, NAKAGAWA, Kazumichi, SUGIMOTO, Manabu, YAMAGICHI, Akihiko, YOKOBORI, Shin-ichi, BESSHO, Yoshitaka, KATOH, Hiroshi, ABE, Tomoko, ENDO, Izumi, KIMURA, Shunta, ONG, Midori, OHMORI, Masayuki, FUJITA, Tomomichi, SUZUKI, TOshisada, ASANO, Maki, OKUDAIRA, Kyoko, and IMAI, Eiichi

Abstract

第36回宇宙環境利用シンポジウム (2022年1月18日-19日. オンライン開催), Space Utilization Research (January 18-19, 2022. Online Meeting), 著者人数: 23名ほか, 資料番号: SA6000168002, G-01

Published
2022

31. Responsible Gene Analysis of Phenotypic Mutants Revealed the Linear Energy Transfer (LET)-Dependent Mutation Spectrum in Rice

Author

Morita, Ryouhei, primary, Ichida, Hiroyuki, additional, Hayashi, Yoriko, additional, Ishii, Kotaro, additional, Shirakawa, Yuki, additional, Usuda-Kogure, Sachiko, additional, Ichinose, Katsunori, additional, Hatashita, Masanori, additional, Takagi, Keiichi, additional, Miura, Kotaro, additional, Kusajima, Miyuki, additional, Nakashita, Hideo, additional, Endo, Takashi, additional, Tojo, Yuka, additional, Okumoto, Yutaka, additional, Sato, Tadashi, additional, Toriyama, Kinya, additional, and Abe, Tomoko, additional

Published
2021
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38. CLAVATA3-like Gene Acts as a Gynoecium Suppression Function in White Campion.

Author

Kazama, Yusuke, Kitoh, Moe, Kobayashi, Taiki, Ishii, Kotaro, Krasovec, Marc, Yasui, Yasuo, Abe, Tomoko, Kawano, Shigeyuki, and Filatov, Dmitry A

Subjects
Y chromosome, X chromosome, GYNOECIUM, SILENE (Genus), SEX chromosomes, FEMALE infertility, GENES
Abstract

How do separate sexes originate and evolve? Plants provide many opportunities to address this question as they have diverse mating systems and separate sexes (dioecy) that evolved many times independently. The classic "two-factor" model for evolution of separate sexes proposes that males and females can evolve from hermaphrodites via the spread of male and female sterility mutations that turn hermaphrodites into females and males, respectively. This widely accepted model was inspired by early genetic work in dioecious white campion (Silene latifolia) that revealed the presence of two sex-determining factors on the Y-chromosome, though the actual genes remained unknown. Here, we report identification and functional analysis of the putative sex-determining gene in S. latifolia , corresponding to the gynoecium suppression factor (GSF). We demonstrate that GSF likely corresponds to a Y-linked CLV3 -like gene that is specifically expressed in early male flower buds and encodes the protein that suppresses gynoecium development in S. latifolia. Interestingly, GSFY has a dysfunctional X-linked homolog (GSFX) and their synonymous divergence (d S  = 17.9%) is consistent with the age of sex chromosomes in this species. We propose that female development in S. latifolia is controlled via the WUSCHEL-CLAVATA feedback loop, with the X-linked WUSCHEL -like and Y-linked CLV3 -like genes, respectively. Evolution of dioecy in the S. latifolia ancestor likely involved inclusion of ancestral GSFY into the nonrecombining region on the nascent Y-chromosome and GSFX loss of function, which resulted in disbalance of the WUSCHEL-CLAVATA feedback loop between the sexes and ensured gynoecium suppression in males. [ABSTRACT FROM AUTHOR]

Published
2022
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39. Rare occurrence of cryptic 5' splice sites by downstream 3' splice site/exon boundary mutations in a heavy-ion-induced egy1-4 allele of Arabidopsis thaliana .

Author

Sanjaya A, Nishijima R, Fujii Y, Asano M, Ishii K, Kazama Y, Abe T, and Fujiwara MT

Abstract

Pre-mRNA splicing is a fundamental process in eukaryotic gene expression, and the mechanism of intron definition, involving the recognition of the canonical GU (5'-splice site) and AG (3'-splice site) dinucleotides by splicing factors, has been postulated for most cases of splicing initiation in plants. Splice site mutations have played crucial roles in unraveling the mechanism of pre-mRNA splicing in planta . Typically, splice site mutations abolish splicing events or activate one or more cryptic splice sites surrounding the mutated region. In this report, we investigated the splicing pattern of the EGY1 gene in an Ar-ion-induced egy1-4 allele of Arabidopsis thaliana . egy1-4 has an AG-to-AC mutation in the 3'-end of intron 3, along with 4-bp substitutions and a 5-bp deletion in adjacent exon 4. RT-PCR, cDNA cloning, and amplicon sequencing analyses of EGY1 revealed that while most wild-type EGY1 mRNAs had a single splicing pattern, egy1-4 mRNAs had multiple splicing defects. Almost half of EGY1 transcripts showed 'intron retention' at intron 3, while the other half exhibited activation of 3' cryptic splice sites either upstream or downstream of the original 3'-splice site. Unexpectedly, around 8% of EGY1 transcripts in egy1-4 exhibited activation of cryptic 5'-splice sites positioned upstream of the authentic 5'-splice site of intron 3. Whole genome resequencing of egy1-4 indicated that it has no other known impactful mutations. These results may provide a rare, but real case of activation of cryptic 5'-splice sites by downstream 3'-splice site/exon mutations in planta ., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Sanjaya, Nishijima, Fujii, Asano, Ishii, Kazama, Abe and Fujiwara.)

Published
2024
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