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ABSTRACT Hydrocephalus is an important neurological disorder rarely described in birds. The present report aims to describe a natural case of hydrocephalus in a blue-fronted parrot (Amazona aestiva) from a conservationist breeding (Minas Gerais, Brazil), clinically affected with neurological signs. The post-mortem lesions noted were megalencephaly, distension and thinning of the skullcap, underdeveloped brain, thickening of the meninges and the presence of a large volume of approximately 10mL of clear cerebrospinal fluid (CSF). The histopathological evaluations demonstrated discrete multifocal malacia, associated with gliosis and neuronal satellitosis in the central nervous system. The screening for viruses revealed the absence of several common etiologies, including Alphavirus (genus universal primers), avian Bornavirus, Alphaherpesvirinae (subfamily universal primers), Paramyxovirus (family universal primers), Newcastle’s disease virus (avian Orthoavulavirus 1), influenza A virus (Alphainfluenzavirus), Marek's disease virus (Mardivirus, Gallid herpesvirus 2), West Nile virus, Flavivirus (Orthoflavivirus universal primers), Apicomplexa (universal primers) and Toxoplasma gondii, all tested negative by PCR or RT-PCR. The aerobic bacteriology and mycology of the CSF did not reveal growth. The results suggest that the chronic accumulation of CSF with increased cranial pressure resulted in distension of the skull, possibly due to congenital causes of increased production, drainage or circulation.

Background: Gliadins have aroused significant interest in the last decade as suitable biomaterials for food and pharmaceutical applications. In particular, the oral route is the preferred method of administration for gliadin-based formulations, due to the affinity of this biomaterial for the gut mucosa. However, up to now, this has been demonstrated only by means of in vivo or ex vivo studies. Methods: This is why, in this study, various in vitro techniques were employed in order to evaluate the ability of polymeric nanoparticles, made up of a commercial grade of the protein and an etheric surfactant, to interact with porcine gastric mucin. The nanosystems were also used for the encapsulation of thiamine hydrochloride, used as a model of a micronutrient. Results: The resulting systems were characterized by a mean diameter of ~160–170 nm, a narrow size distribution when 0.2–0.6 mg/mL of thiamine was used, and an encapsulation efficiency between 30 and 45% of the drug initially employed. The incubation of the gliadin nanosystems with various concentrations of porcine gastric mucin evidenced the ability of the carriers to interact with the mucus glycoprotein, showing a decreased Zeta potential after a 4 h incubation (from ~−30 to −40 mV), while demonstrating that the encapsulation of the drug did not affect its bioadhesive features. Conclusions: Altogether, these data support the conceivable application of gliadin nanoparticles as formulations for the oral administration of bioactive compounds. [ABSTRACT FROM AUTHOR]

The rapid resistance developed by pathogenic microorganisms against the current antimicrobial pool represents a serious global public health problem, leading to the search for new antibiotic agents. The scorpion Tityus stigmurus, an abundant species in Northeastern Brazil, presents a rich arsenal of bioactive molecules in its venom, with high potential for biotechnological applications. However, venom cytotoxicity constitutes a barrier to the therapeutic application of its different components. The objective of this study was to produce T. stigmurus-venom-loaded cross-linked chitosan nanoparticles (Tsv/CN) at concentrations of 0.5% and 1.0% to improve their biological antimicrobial activity. Polymeric nanoparticles were formed with a homogeneous particle size and spherical shape. Experimental formulation parameters were verified in relation to mean size (<180 nm), zeta potential, polydispersity index and encapsulation efficiency (>78%). Tsv/CN 1.0% demonstrated an ability to increase the antimicrobial venom effect against Staphylococcus aureus bacteria, exhibiting an MIC value of 44.6 μg/mL. It also inhibited different yeast species of the Candida genus, and Tsv/CN 0.5% and 1.0% led to a greater inhibitory effect of C. tropicalis and C. parapsilosis strains, presenting MIC values between 22.2 and 5.5 µg/mL, respectively. These data demonstrate the biotechnological potential of these nanosystems to obtain a new therapeutic agent with potential antimicrobial activity. [ABSTRACT FROM AUTHOR]

Stress shielding is a problem for traditional metal bone fixation plates made of magnesium and titanium alloys. This problem can be solved by using composite materials with a low elastic modulus. This study analyzed the effect of carbon fiber reinforced PEEK (CFRP) composites on stress shielding under static loading using finite element simulations. Callus formation times relative to the healing period were gradually imposed according to the elapsed time, considering 1% and 75% as healing stages. The Inventor© 3D CAD 2024 software was used for modeling, and the ANSYS© FEA R2023 software was used for analysis. The results showed that metal fixation plates made of titanium and magnesium alloys transferred less stress to the bone than the CFRP fixation plate. In particular, the use of the CFRP fixation plate resulted in a higher peak stress and a more uniform stress field in the bone, especially in the bone-plate contact area, where the risk of stress shielding is higher in the 1% and 75% healing phases. [ABSTRACT FROM AUTHOR]

As a wider range of organizations explore using data science systems, data science research has given growing attention to the role of domain experts. Most of this research still views data science systems as centered on the development of statistical models or algorithms by technical data scientists, with domain experts limited to the role of informers. Our paper turns attention to how domain experts mediate whether data science models or algorithms lead to action through their situated data practices. Drawing on ethnographic fieldwork and a pilot machine learning project at a craft brewery, we identify situations where the brewers' data practices led to unreliable, incomplete data, and unpack how such data limited the effectiveness of data science activities. Extending research in CSCW and STS on domain experts' data practices to the data science context, we aim to inform the design of data science systems that are more actionable for their end-users. [ABSTRACT FROM AUTHOR]

Wildfires burn annually over 400,000 ha in Mediterranean countries. By the end of the 21st century, wildfire Burnt Area (BA) and associated Green House Gas (GHG) emissions may double to triple due to climate change. Regional projections of future BA are urgently required to update wildfire policies. We present a robust methodology for estimating regional wildfire BA and GHG emissions under future climate change scenarios in the Mediterranean. The Fire Weather Index, selected drought indices, and meteorological variables were correlated against BA/GHG emissions data to create area-specific statistical projection models. State-of-the-art regional climate models (horizontal resolution: 12 km), developed within the EURO-CORDEX initiative, simulated data under three climate change scenarios (RCP2.6, RCP4.5, and RCP8.5) up to 2070. These data drove the statistical models to estimate future wildfire BA and GHG emissions in three pilot areas in Greece, Montenegro, and France. Wildfire BA is projected to increase by 20% to 130% up to 2070, depending on the study area and climate scenario. The future expansion of fire-prone areas into the north Mediterranean and mountain environments is particularly alarming, given the large biomass present here. Fire-smart landscape management may, however, greatly reduce the projected future wildfire BA and GHG increases. [ABSTRACT FROM AUTHOR]

Smart manufacturing is a popular concept for smarter decision-making and more efficient production. Although distributed methods for data management and processing in smart manufacturing have many advantages such as low cost of adaptation and convenience for local database, some methods are hard to manage variable data sources and discover proper range of data for smart decision-making. Therefore, Dataspace is considered in this article to be a feasible and effective method. From the relation-defined perspective of utilisation of industrial Big Data, the contribution is a novel industrial Dataspace design with static structure and working flow paths for smart manufacturing. In design, the industrial Dataspace platform has been proposed to accommodate smart manufacturing characteristics with the intelligence of pay-as-you-go, like harnessing distributed heterogenous data from industrial enterprises, understanding industrial data by ontology or knowledge, corelating the data with smart applications, and enabling related decisions. A further analytical case in Surface Mounting Technology manufacturing of welding procedure is provided to illustrate the execution of customisation, focused and related decision support, and system evolution within industrial Dataspace. [ABSTRACT FROM AUTHOR]

Biological denitrification is a generally economically viable and reliable technology for nitrate (NO3−-N) and nitrite (NO2−-N) removal from wastewater. Nitrate/nitrite reduction requires an electron donor, which can be supplied from influent wastewater organic compounds, added as an external carbon source, or by endogenous respiration. Decisions regarding the selection of an external carbon source should take into consideration its cost, sludge generation quantity, the rate and efficiency of denitrification, and chemical storage safety. The expense of waste management and external carbon sources accounts for up to 50% of the overall cost of wastewater treatment. The carbon source characteristics required for biological denitrification affect the microbial community structure, denitrification rate, and intermediate products. This review is based on a bibliometric analysis and a systematic literature review providing information and insight into the topic of the denitrification process using different carbon sources. In this review, the main focus was on discussing alternative carbon sources vs. traditional carbon sources in terms of the carbon source price, C/N ratio, denitrification efficiency and rate, operational parameters, and advantages and disadvantages, as well as the limitations in the denitrification process. Future perspectives may include the operating parameters influencing the stability of the removal performance; the maintenance and improvement of nitrate removal; a study of the diversity of the microbial community; research on the application of new alternative carbon sources in denitrification; and N2O emission detection and minimisation. [ABSTRACT FROM AUTHOR]

Gaining insight into the interplay between crucial fertilizers and their impact on crop characteristics is crucial for enhancing the agricultural output and promoting sustainable crop administration. The objective of this study was to assess the growth, productivity, and nutrient-use efficiency (NUE) of common bean genotypes with varying levels of NPK. In the RCBD field study, three genotypes of common bean were cultivated—SKUA-WB-5000/1446 (V1), SKUA-WB-5002/185 (V2), and SKUA-WB-5003/1492 (V3)—together with six combinations of NPK (N2:P2O5:K2O kg ha−1). The findings indicated that the height of the plants had a positive correlation with elevated NPK levels subsequent to the maximum point in NPK 10-20-10. The V1 genotype exhibited superior growth and productive traits, particularly with regard to its higher seed index and much greater quantity of pods plant−1. This indicates that the V1 genotype may be a suitable choice for cultivating common beans and highlights the importance of adjusting nutrient levels to ensure sustainable crop management. This study suggests applying 30-60-30 NPK kg ha−1 of fertilizer for genotypes V1 and V3, while genotype V2 should receive 40-80-40 NPK kg ha−1 under rainfed circumstances. [ABSTRACT FROM AUTHOR]

Corn (Zea mays) is the most widely planted crop in the world. Dalbulus maidis (Hemiptera: Cicadellidae) is currently a primary corn pest. The starting point for the development of pest control decision-making systems is the determination of a conventional sampling plan. Therefore, this study aimed to determine a practical conventional sampling plan for D. maidis in corn crops. Insect density was evaluated in 28 commercial fields. Subsequently, D. maidis densities were sampled from fields ranging from 1 to 100 ha. Insect density conformed to a negative binomial distribution in 89.29% of the fields. The insect densities determined using the sampling plan had a low error rate (up to 15%). Sampling time and costs ranged from 2.06 to 39.45 min/ha and 0.09 to 1.81 USD/ha for fields of 1–100 ha, respectively. These results provide the first precise and representative conventional sampling plan for scouting D. maidis adults grown in corn fields. Therefore, the conventional sampling plan for D. maidis determined in this study is practical and can be incorporated into integrated pest management programs for corn crops owing to its representativeness, precision, speed, and low cost. [ABSTRACT FROM AUTHOR]

Breast cancer is the most common cancer among women in the world as well as in the United States. Molecular and histological differentiation have helped clinicians optimize treatments with various therapeutics, including hormonal therapy, chemotherapy, immunotherapy, and radiation therapy. Recently, immunotherapy has become the standard of care in locally advanced triple-negative breast cancer and an option across molecular subtypes for tumors with a high tumor mutation burden. Despite the advancements in personalized medicine directing the management of localized and advanced breast cancers, the emergence of resistance to these therapies is the leading cause of death among breast cancer patients. Therefore, there is a critical need to identify and validate predictive biomarkers to direct treatment selection, identify potential responders, and detect emerging resistance to standard therapies. Areas of active scientific and clinical research include novel personalized and predictive biomarkers incorporating tumor microenvironment, tumor immune profiling, molecular characterization, and histopathological differentiation to predict response and the potential emergence of resistance. [ABSTRACT FROM AUTHOR]

This is a single-center retrospective study to assess the safety and tolerability of continuous inhaled iloprost use as rescue therapy for refractory pulmonary hypertension (PH) in critically ill neonates and infants. A retrospective chart review was performed on 58 infants and data were collected at baseline, 1, 6, 12, 24, 48 and 72 h of iloprost initiation. Primary outcomes were change in heart rate (HR), fraction of inspired oxygen (FiO2), mean airway pressures (MAP), blood pressure (BP) and oxygenation index (OI). Secondary outcomes were need for extracorporeal membrane oxygenation (ECMO) and death. 51 patients treated for >6 h were analyzed in 2 age groups, neonate (≤28 days: n = 32) and infant (29–365 days: n = 19). FiO2 (p < 0.001) and OI (p = 0.01) decreased, while there were no significant changes in MAP, BP and HR. Of the fifteen patients placed on ECMO, seven were bridged off ECMO on iloprost and eight died. Twenty-four out of fifty-one patients (47%) recovered without requiring ECMO, while twelve (23%) died. Iloprost as add-on therapy for refractory PH in critically ill infants in the NICU has an acceptable tolerability and safety profile. Large prospective multicenter studies using iloprost in the neonatal ICU are necessary to validate these results. [ABSTRACT FROM AUTHOR]

We aimed to analyze the usefulness of the 6–20 rating of perceived exertion (RPE) scale for prescribing and self-regulating high-intensity interval (HIIE) and moderate-intensity continuous (MICE) aerobic exercise performed in a heated swimming pool (32 °C). Fifteen older individuals (65 ± 3 years) treated for hypertension underwent a symptom-limited maximal graded exercise test to determine their heart rate at anaerobic threshold, and respiratory compensation point. On different days, participants were randomized to HIIE (walking/jogging between 11 and 17 of RPE; 25 min) and MICE (walking at 11–13 of RPE; 30 min). Heart rate during the low-intensity intervals of HIIE and MICE remained below the graded exercise test's heart rate at anaerobic threshold (−7 ± 18 bpm/−16 ± 15 bpm) and respiratory compensation point (−18 ± 18 bpm/−30 ± 16 bpm), respectively, and maintained in the aerobic training zone during the high-intensity intervals of HIIE (+8 ± 18 bpm/−4 ± 19 bpm). The RPE scale at 15–17 is a useful tool for prescribing and self-regulating heated water-based HIIE and may have important implications for water-based exercise in older individuals with hypertension. [ABSTRACT FROM AUTHOR]

This study investigated the potential of microbial fermentative transforming processes in valorizing the cashew apple by-product into a low-alcohol, health-benefiting beverage. We particularly investigated the use of a non-Saccharomyces yeast, Cyberlindnera rhodanensis DK, as the main targeted microbe. At 30 °C without agitation, C. rhodanensis DK caused changes in key parameters during the fermentation of cashew apple juice (CAJ) in terms of varied pH values and initial sugar concentrations. This result indicated that pure CAJ, with pH adjusted to 6 and with the original 6.85% (w/v) total sugar content, was the most feasible condition, as glucose and fructose were mostly consumed at 12 days of fermentation. A co-culture approach with either Saccharomyces cerevisiae TISTR 5088 or Lactobacillus pentosus A14-6 was investigated to improve both physicochemical and fermentation characteristics. Co-fermentation with S. cerevisiae TISTR 5088 resulted in significantly increased ethanol accumulation to 33.61 ± 0.11 g/L, but diminished bioactive compounds, antioxidant activity, and antidiabetic potential. In contrast, co-fermentation with L. pentosus A14-6 demonstrated excellent outcomes, as it significantly increased sugar consumption and finally remained at only 4.95 g/L compared to C. rhodanensis DK alone, produced lower levels of ethanol at only 19.47 ± 0.06 g/L, and higher total titratable acid (TTA), resulting in a final pH of 3.6. In addition, co-fermentation with this lactic acid bacterium significantly enhanced bioactive compounds and antioxidant activity and also retained potential antidiabetic properties. These findings highlight the feasibility of using tailored microbial fermentation strategies to produce low-alcohol beverages with enhanced health-promoting properties from CAJ; however, product-development processes following health food regulations and sensory evaluation are necessary. [ABSTRACT FROM AUTHOR]

In this study, an ecological framework was developed to sort out the existing forest fire prevention techniques. The subsequent analysis involved comparing the ecological values and application prospects of these techniques developed in different time periods. As ecological applications, fire regimes reflect vegetation response to wildfires, providing valuable insights for shaping the fire risk and behaviors in forests through fuel treatment and vegetation modification. Fuel treatment and the construction of green fire barriers are both rooted in existing ecosystems and possess ecological characteristics. While fuel thinning focuses on reducing the potential fire intensity and severity, green fire barriers have been more targeted for fire prevention purposes. Among these techniques, green fire barriers demonstrate unique sustainability and have the potential to generate long-term ecological and environmental benefits. Through the comprehensive utilization of several fuel management formulas, we can effectively combine the fire prevention demands with ecological maintenance and environment protection. This integrated approach promotes the development of fire-resilient ecosystems and desirable living environments in a more realistic and sustainable manner. [ABSTRACT FROM AUTHOR]

Large quantities of wine lees are produced annually by the wine industry. The high phenolic content makes them unsuitable for disposal in the environment or animal feed without a suitable treatment. In this study, wine lees were treated by Pleurotus ostreatus in submerged cultivation, producing a high-value biomass and elevated levels of laccase, an important industrial enzyme. Biomass and laccase production reached 21 g/L and 74,000 Units/L, respectively, at the optimal conditions of initial pH 6.0, 20% v/v wine lees, 30 g/L glucose, and 20 g/L yeast extract, while decolorization and dephenolization rates of the waste were over 90%. The mycelial biomass was rich in proteins and essential amino acids reaching up to 43% and 16% per dry weight, respectively. Carbohydrates and lipids were the second richest bioactive compound in biomass, with values of 29.4 ± 2.7% and 29.5 ± 2.7%, respectively. The crude laccase in the culture supernatant was purified via a simple two-step purification procedure by 4.4-fold with a recovery of 44%. The molecular weight of the enzyme was determined to be 62 kDa via SDS electrophoresis. Enzyme activity was optimal at pH 5.0 and 70 °C. The activation energy of the enzyme was calculated at a value of 20.0 ± 0.2 kJ/mol. The pH stability and thermostability of the purified laccase were studied. The enzyme was remarkably stable at pH 8.0 and at temperatures up to 40 °C. The thermal inactivation energy of the enzyme was determined to be 76.0 ± 1.2 kJ/mol. The thermodynamic parameters (ΔH*, ΔG*, and ΔS*) for the thermal deactivation of the purified laccase at a temperature range of 20–60 °C were: 73.8 ≤ ΔH* ≤ 74.3 kJ·mol−1, 98.7 ≤ ΔG* ≤ 101.9 kJ·mol−1, and −90.5 ≤ ΔS* ≤ −84.3 J·mol−1·K−1. Wine lees could be ideal substrates of fungal cultivation for laccase production and biomass with a high protein content in an eco-friendlier way. [ABSTRACT FROM AUTHOR]

In the present work, the powder metallurgy (PM) technique was used to synthesize biocompatible alloys Mg95–Zn5, Mg90–Zn10, and Mg85–Zn15 (wt %) under an argon atmosphere that employed stainless-steel vessels and spheres with a milling treatment of 360 rpm during 15 h. The obtained powder was consolidated through a sintering process for subsequent ion nitriding treatment to increase its corrosion resistance and hardness. The synthesized alloys analyzed by Scanning electron microscopy (SEM) images showed a reduction in particle size with increasing grinding time. The X-ray diffraction (XRD) results showed the formation of a MgZn intermetallic phase and, furthermore, that no impurities were found during the grinding process. The surface-modified Mg–Zn alloys showed some improvement in terms of corrosion (Ecorr 26% and Icorr 13%) and microhardness (HV 40%) compared to Mg. Cytotoxicity evaluation was conducted via an MTT ((3-(4,5-DIMETHYLTHIAZOL-2-YL)-2,5-DIPHENYLTETRAZOLIUM BROMIDE)) assay, which revealed that the Mg–Zn alloys and nitrided samples did not exhibit cytotoxicity towards fibroblast cells after 96 h. [ABSTRACT FROM AUTHOR]

This study aims to address the challenges associated with conventional metallic bone fixation plates in biomechanical applications, such as stainless steel and titanium alloys, including stress shielding, allergic reactions, corrosion resistance, and interference with medical imaging. The use of materials with a low elastic modulus is regarded as an effective approach to overcome these problems. In this study, the impact of different types of chopped carbon fiber-reinforced polyether ether ketone (CCF/PEEK) functionally graded material (FGM) bone plates on stress shielding under static and instantaneous dynamic loading was explored using finite element analysis (FEA). The FGM bone plate models were established using ABAQUS and the user's subroutine USDFLD and VUSDFLD, and each model was established with an equivalent overall elastic modulus and distinctive distributions. The results revealed that all FGM bone plates exhibited lower stress shielding effects compared to metal bone plates. Particularly, the FGM plate with an elastic modulus gradually increased from the centre to both sides and provided maximum stress stimulation and the most uniform stress distribution within the fractured area. These findings offer crucial insights for designing implantable medical devices that possess enhanced mechanical adaptability. [ABSTRACT FROM AUTHOR]

Chagas disease is a neglected infectious disease caused by the protozoan Trypanosoma cruzi, primarily transmitted by triatomine vectors, and it threatens approximately seventy-five million people worldwide. This parasite undergoes a complex life cycle, transitioning between hosts and shifting from extracellular to intracellular stages. To ensure its survival in these diverse environments, T. cruzi undergoes extreme morphological and molecular changes. The metacyclic trypomastigote (MT) form, which arises from the metacyclogenesis (MTG) process in the triatomine hindgut, serves as a crucial link between the insect and human hosts and can be considered the starting point of Chagas disease. This review provides an overview of the current knowledge regarding the parasite's life cycle, molecular pathways, and mechanisms involved in metabolic and morphological adaptations during MTG, enabling the MT to evade the immune system and successfully infect human cells. [ABSTRACT FROM AUTHOR]

"Green" strategies to build up novel organic nanocarriers with bioperformance are modern trends in nanotechnology. In this way, the valorization of bio-wastes and the use of living systems to develop multifunctional organic and biogenic nanocarriers (OBNs) have revolutionized the nanotechnological and biomedical fields. This paper is a comprehensive review related to OBNs for bioactives' delivery, providing an overview of the reports on the past two decades. In the first part, several classes of bioactive compounds and their therapeutic role are briefly presented. A broad section is dedicated to the main categories of organic and biogenic nanocarriers. The major challenges regarding the eco-design and the fate of OBNs are suggested to overcome some toxicity-related drawbacks. Future directions and opportunities, and finding "green" solutions for solving the problems related to nanocarriers, are outlined in the final of this paper. We believe that through this review, we will capture the attention of the readers and will open new perspectives for new solutions/ideas for the discovery of more efficient and "green" ways in developing novel bioperformant nanocarriers for transporting bioactive agents. [ABSTRACT FROM AUTHOR]

Alzheimer's disease (AD) is the main neurodegenerative disorder characterized by several pathophysiological features, including the misfolding of the tau protein and the amyloid beta (Aβ) peptide, neuroinflammation, oxidative stress, synaptic dysfunction, metabolic alterations, and cognitive impairment. These mechanisms collectively contribute to neurodegeneration, necessitating the exploration of therapeutic approaches with multiple targets. Physical exercise has emerged as a promising non-pharmacological intervention for AD, with demonstrated effects on promoting neurogenesis, activating neurotrophic factors, reducing Aβ aggregates, minimizing the formation of neurofibrillary tangles (NFTs), dampening inflammatory processes, mitigating oxidative stress, and improving the functionality of the neurovascular unit (NVU). Overall, the neuroprotective effects of exercise are not singular, but are multi-targets. Numerous studies have investigated physical exercise's potential in both AD patients and animal models, employing various exercise protocols to elucidate the underlying neurobiological mechanisms and effects. The objective of this review is to analyze the neurological therapeutic effects of these exercise protocols in animal models and compare them with studies conducted in AD patients. By translating findings from different approaches, this review aims to identify opportune, specific, and personalized therapeutic windows, thus advancing research on the use of physical exercise with AD patients. [ABSTRACT FROM AUTHOR]

The benefits of maternal physical activity during pregnancy are well documented, but long-term effects on the child have been less studied. Therefore, we conducted a pilot follow-up study of a lifestyle intervention during pregnancy that aimed to investigate whether exercise (endurance and strength training) during pregnancy affects motor performance and body composition of children up to 9 years of age, as well as possible influencing factors like brain-derived neurotrophic factor (BDNF) and lifestyle. Eleven mother−child pairs from the intervention and eight mother−child pairs from the control group were included. From birth up to 9 years of age, no differences in body mass index (BMI) or body mass index standard deviation scores (BMI-SDS) were found between the groups. Lifestyle intervention was one of the influencing factors for children's cardiorespiratory endurance capacity and coordination. Moreover, maternal BDNF in the last trimester was significantly associated with running performance, which may be due to better neuronal development. This is the first study evaluating the effects of a lifestyle intervention during pregnancy on the motor performance 9 years after birth. Children's participation in exercise programs over the past 9 years was not continuously recorded and therefore not included in the analysis. Even a cautious interpretation of these results indicates that a healthy lifestyle during pregnancy is essential in promoting child health. Larger studies and randomized control trials are necessary to confirm our results, especially those pertaining to the role of BDNF. [ABSTRACT FROM AUTHOR]

Trypanosoma cruzi, the agent of Chagas disease, must adapt to a diversity of environmental conditions that it faces during its life cycle. The adaptation to these changes is mediated by signaling pathways that coordinate the cellular responses to the new environmental settings. Cyclic AMP (cAMP) and Calcium (Ca2+) signaling pathways regulate critical cellular processes in this parasite, such as differentiation, osmoregulation, host cell invasion and cell bioenergetics. Although the use of CRISPR/Cas9 technology prompted reverse genetics approaches for functional analysis in T. cruzi, it is still necessary to expand the toolbox for genome editing in this parasite, as for example to perform multigene analysis. Here we used an efficient T7RNAP/Cas9 strategy to tag and delete three genes predicted to be involved in cAMP and Ca2+ signaling pathways: a putative Ca2+/calmodulin‐dependent protein kinase (CAMK), Flagellar Member 6 (FLAM6) and Cyclic nucleotide‐binding domain/C2 domain‐containing protein (CC2CP). We endogenously tagged these three genes and determined the subcellular localization of the tagged proteins. Furthermore, the strategy used to knockout these genes allows us to presume that TcCC2CP is an essential gene in T. cruzi epimastigotes. Our results will open new venues for future research on the role of these proteins in T. cruzi. [ABSTRACT FROM AUTHOR]

Sporothrix brasiliensis is an emerging fungal pathogen causing cat-transmitted sporotrichosis, an epi-zoonosis affecting humans, cats and dogs in Brazil and now spreading to neighboring South American countries. Here, we report the first two autochthonous cases of cat-transmitted sporotrichosis in Paraguay. The first case was a four-year-old male cat showing several ulcerative lesions, nasal deformity and respiratory symptoms. The second case was a one-year-old male cat showing a single ulcerated lesion, respiratory symptoms and nasal deformity. Both cases were admitted to a veterinary clinic in Ciudad del Este, Paraguay. Isolates were recovered from swabs of the two cases. Using molecular methods, the isolates were identified as S. brasiliensis. [ABSTRACT FROM AUTHOR]

Urban areas adjacent to wildlands are very dangerous zones for residents and their properties during a wildfire event. We attempted to connect wildfire simulations with field inventories and surveys to create a framework that can be used to enhance the fire resistance of residential structures located in the wildland-urban interface (WUI). Legal restrictions and the lack of economic incentives for WUI residents greatly limit the potential to appropriately intervene to enhance their property's fire resistance. By studying in situ the resilience of building materials and combining them with exposure metrics produced from wildfire simulations, we created an index that helps to assess fire risk at the property level. The proposed index can support property owners to optimally manage the vegetation near or inside their property. State agencies can use our proposed index to estimate with a consistent methodology which properties are more exposed and with higher risk from fire damage so that specific fuel and vegetation management practices on and around them can be suggested or enforced. [ABSTRACT FROM AUTHOR]

FIRELAN was developed as a model expected to foster the resilience to fire and sustainability of a landscape that is based on a number of premises about fire behaviour. We critically review FIRELAN and find that flawed ecological concepts and terminology are used, and that six fallacies are pervasive throughout the paper, namely begging the question regarding the effectiveness of land cover changes; the appeal to nature on the preference of native species over non-native species; confirmation bias on the flammability of native vs. non-native species; the oversimplification of fire behaviour drivers; questionable causation regarding the effect of land cover on fire hazard; and non-sequitur in respect to the flammability–resilience relationship. We conclude that FIRELAN overall lacks supporting scientific evidence, both theoretical and empirical, and would be unable to deliver adequate wildfire mitigation. Recommendations are given to guide the landscape-level process of planning and implementing wildfire impacts mitigation. [ABSTRACT FROM AUTHOR]

Climate and land-use changes have been contributing to the increase in the occurrence of extreme wildfires, shifting fire regimes and driving desertification, particularly in Mediterranean-climate regions. However, few studies have researched the influence of land use/cover on fire regimes and carbon storage at the broad national scale. To address this gap, we used spatially explicit data from annual burned areas in mainland Portugal to build a typology of fire regimes based on the accumulated burned area and its temporal concentration (Gini Index) between 1984 and 2019. This typology was then combined with carbon stock data and different landscapes to explore relationships between landscape types and two important ecosystem services: wildfire reduction and carbon stock. Multivariate analyses were performed on these data and the results revealed a strong relationship between landscapes dominated by maritime pine and eucalypt plantations and highly hazardous fire regimes, which in turn hold the highest carbon stocks. Shrubland and mixed landscapes were associated with low carbon stocks and less hazardous fire regimes. Specialized agricultural landscapes, as well as mixed native forests and mixed agroforestry landscapes, were the least associated with wildfires. In the case of agricultural landscapes, however, this good wildfire performance is achieved at the cost of the poorest carbon stock, whereas native forests and agroforestry landscapes strike the best trade-off between carbon stock and fire regime. Our findings support how nature-based solutions promoting wildfire mitigation and carbon stock ecosystem services may prevent and revert land degradation harming Mediterranean regions. [ABSTRACT FROM AUTHOR]

Notch signaling is an evolutionarily conserved pathway which functions between adjacent cells to establish their distinct identities. Despite operating in a simple mechanism, Notch signaling plays remarkably diverse roles in development to regulate cell fate determination, organ growth and tissue patterning. While initially discovered and characterized in the model insect Drosophila melanogaster, recent studies across various insect species have revealed the broad involvement of Notch signaling in shaping insect tissues. This review focuses on providing a comprehensive picture regarding the roles of the Notch pathway in insect development. The roles of Notch in the formation and patterning of the insect embryo, wing, leg, ovary and several specific structures, as well as in physiological responses, are summarized. These results are discussed within the developmental context, aiming to deepen our understanding of the diversified functions of the Notch signaling pathway in different insect species. [ABSTRACT FROM AUTHOR]

Elevated plasma lipoprotein(a) [Lp(a)] is a relatively common and highly heritable trait conferring individuals time-dependent risk of developing atherosclerotic cardiovascular disease (CVD). Following its first description, Lp(a) triggered enormous scientific interest in the late 1980s, subsequently dampened in the mid-1990s by controversial findings of some prospective studies. It was only in the last decade that a large body of evidence has provided strong arguments for a causal and independent association between elevated Lp(a) levels and CVD, causing renewed interest in this lipoprotein as an emerging risk factor with a likely contribution to cardiovascular residual risk. Accordingly, the 2022 consensus statement of the European Atherosclerosis Society has suggested inclusion of Lp(a) measurement in global risk estimation. The development of highly effective Lp(a)-lowering drugs (e.g., antisense oligonucleotides and small interfering RNA, both blocking LPA gene expression) which are still under assessment in phase 3 trials, will provide a unique opportunity to reduce "residual cardiovascular risk" in high-risk populations, including patients with arterial hypertension. The current evidence in support of a specific role of Lp(a) in hypertension is somehow controversial and this narrative review aims to overview the general mechanisms relating Lp(a) to blood pressure regulation and hypertension-related cardiovascular and renal damage. [ABSTRACT FROM AUTHOR]

Due to the associated fire risk, the wildland–urban interface (WUI) has drawn the attention of researchers and managers from a range of backgrounds. From a land management point of view, it is important to identify the WUI to determine areas to prioritise for fire risk prevention. It is also important to know the fire risk mitigation measures available to select the most appropriate for each specific context. In this systematic review, definitions of the WUI were investigated and physical mitigation measures for reducing the risk of fire were examined from a land management perspective. The PRISMA 2020 Statement was applied to records published until 31 December 2022 and retrieved from the Web of Science, Scopus, and other research engines. A total of 162 publications from scientific journals and the grey literature were scrutinised and selected for analysis. Only publications providing an original definition of the WUI or proposing physical measures to reduce fire risk at the interface were retained, while those relating to emergency management and social perception were not considered. The risk of bias was reduced by internal cross-assessment by the research team. Definitions of the WUI (n = 40 publications) changed according to the research objective, varying broadly in identification of the anthropogenic and the wildland components of the interface. Terminology varied according to the definition, and the term wildland–human interface (WHI) was found to be more comprehensive than WUI. Methodological definitions of the interface ranged from using aggregated data through to identification of the buildings at risk in the interface with considerable precision. Five categories of physical fire risk mitigation measures (n = 128 publications) were identified: clearance distances, landscaping, wildland fuel management, land planning, and buildings design and materials. The most effective measures were those applied at early stages of urban development, and maintenance of assets and vegetation is crucial for preparedness. This review represents an analysis of scientific evidence on which land managers can base their actions to reduce the fire hazard risk in the WUI. The number of studies investigating the WUI is considerable, but experimental studies and quantitative results are scarce, and better communication and coordination among research groups and land management agencies is advisable. This systematic review was not registered. [ABSTRACT FROM AUTHOR]

Doxorubicin (DOX) is an effective chemotherapeutic drug; however, its cardiotoxicity and resistance compromise its therapeutic index. The Notch pathway was reported to contribute to DOX cancer resistance. The role of Notch pathway in DOX cardiotoxicity has not been identified yet. Notch receptors are characterized by their extracellular (NECD) and intracellular (NICD) domains (NICD). The γ-secretase enzyme helps in the release of NICD. Dibenzazepine (DBZ) is a γ-secretase inhibitor. The present study investigated the effect of Notch pathway inhibition on DOX cardiotoxicity. Twenty-four male Wistar rats were divided into four groups: control group, DOX group, acute cardiotoxicity was induced by a single dose of DOX (20 mg/kg) i.p., DOX (20 mg/kg) plus DBZ group, and DBZ group. The third and fourth groups received i.p. injection of DBZ daily for 14 days at 2 mg/kg dose. DOX cardiotoxicity increased the level of serum creatine kinase-MB and cardiac troponin I, and it was confirmed by the histopathological examination. Moreover, the antioxidants glutathione peroxidase and superoxide dismutase levels were markedly decreased, and the inflammatory markers, inducible nitric oxide synthase, nuclear factor-ķB, and tumor necrosis factor-α were markedly increased. Furthermore, DOX increased BAX protein and downregulated BCL-2. In addition, DOX upregulated Notch pathway-related parameters: Hes1 and Hey1 mRNA levels, and increased Hes1 protein levels. DBZ ameliorated DOX-induced cardiotoxicity, evidenced by reducing the cardiac injury biomarkers, improving cardiac histopathological changes, correcting antioxidant levels, and reducing inflammatory and apoptotic proteins. Our study indicates the protective effect of Notch inhibitor against DOX-induced cardiotoxicity., (© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

Featured Application: This article presents a numerical simulation model built using COMSOL software to study the thermophysical properties of different breast sizes. Specifically, the effects of varied blood perfusion, tumor size, location depth, and thermal conductivity were studied in relation to breast size. Pennes' bioheat formula was applied to illustrate thermal distribution by applying thermal conductivity characteristics in each layer in the breast using COMSOL software. This was followed by an emulation experiment to demonstrate the effectiveness of using situ-cooling gel on the breast surface area in providing better temperature contrast and amplifying heat detection. Simulation results demonstrated that both large breast size and small breast size both have heat detection issues, while experimental results showed how situ-cooling can help mitigate these issues. The findings offer valuable insights for future research in this field. This article presents a numerical simulation model using COMSOL software to study breast thermophysical properties. It analyzes tumor heat at different locations within the breast, records breast surface temperatures, investigates the effects of factors such as blood perfusion, size, depth, and thermal conductivity on breast size, and applies Pennes' bioheat formula to illustrate thermal distribution on the breast skin surface. An analysis was conducted to examine how changes in tumor location depth, size, metabolism, blood flow, and heat conductivity affect breast skin surface temperature. The simulation model results showed that the highest variations in skin temperatures for breasts with tumors and without tumors can range from 2.58 °C to 0.274 °C. Further, large breast size with a large surface area consistently reduces the temperature variations on the skin and might have difficulty in yielding observable temperature contrast. For small breast sizes, however, heat from tumor sizes below 0.5 cm might be quite difficult to detect, while tumors located deep within the breast layers could not produce observable temperature variations. Motivated by the above interesting results, an emulation experiment was conducted to enhance the observable heat and temperature background contrast, using situ-cooling gel applied to silicon breasts, while the tumor source was emulated using LEDs. The experiment was used to evaluate the effectiveness of adding situ-cooling to the breast surface area, and to study the modulated effect of tumor size and depth. Experimental results showed that situ-cooling enhances thermal contrast in breast thermal images. For example, for a tumor location at a depth of 10 cm, a difference of 6 °C can still be achieved with situ-cooling gel applied, a feat that was not possible in the simulation model. Furthermore, changes in tumor size and location depth significantly impacted surface temperature distribution. [ABSTRACT FROM AUTHOR]

Edible packaging has been a topic of much discussion during recent years, mainly due to its lower environmental impact. This study aimed to investigate the properties of an edible packaging made from a combination of carrageenan, orange essential oil (OEO), and trehalose (Tre) under different humidity conditions. The films were analyzed based on their water content, solubility, and textural properties, such as strength and breaking strain. The results of the study showed that the addition of trehalose reduced the water content and increased the strength of the packaging, regardless of the humidity conditions. The inclusion of orange essential oil also contributed to lower water content, which led to more water-resistant packaging (during standard humidity conditions (45%)—c: 15.31%; Tre3OT80: 4.04%, Tre1OT80: 4.48%). The findings of this study have important implications for the production of stable and environmentally friendly edible packaging. The results demonstrate the potential of trehalose and orange essential oil as additives to enhance the properties of edible packaging, particularly in terms of its resistance to moisture. The best results were found in Tre1OT80 and Tre3OT80 samples. Moreover, the study emphasizes the importance of considering storage conditions in the development of edible packaging, as different humidity levels can significantly affect the packaging's properties and shelf life. The findings have practical applications for the food industry, particularly in the development of sustainable packaging solutions and for further studies where the application of this packaging can be analyzed for different foodstuffs. [ABSTRACT FROM AUTHOR]

Forest fires are becoming a more significant problem in Central Europe, but their danger is not as high as that in Southern Europe. The exception, however, is forest fires occurring in disturbed areas (windthrow and bark beetle outbreak areas), which are comparable in severity and danger to the most serious forest fires. In this study, we describe the current situation in Central European countries in terms of fire protection for disturbed areas in managed forests and forest stands left to spontaneously develop (secondary succession). If a country has regulations and strategies in this area, they are often only published in the local language. In this review, we combine information from all Central European countries and summarize it in a unified international language, provide an opportunity for local authorities to express their own experiences, and integrate data from worldwide scientific research. Thus, this paper may be considered a universal guide for managing fire protection and preparedness in disturbed areas and can serve as a reference for the establishment of strict legislative rules at the state level. These laws must be obligatory for all stakeholders in individual countries. The motivation for this study was two large forest fires in an area left to spontaneously develop in the Bohemian Switzerland National Park in the Czech Republic and Harz Mountains in Germany in the summer of 2022. These incidents revealed that fire prevention legislation was inadequate or nonexistent in these areas. The strategy of the European Union is to increase the size of protected areas and spontaneous development areas. Therefore, we consider it necessary to provide governments with relevant information on this topic to create conditions for better management of these destructive events. [ABSTRACT FROM AUTHOR]

Species of the genus Kalanchoe have a long history of therapeutic use in ethnomedicine linked to their remarkable healing properties. Several species have chemical and anatomical similarities, often leading to confusion when they are used in folk medicine. This review aims to provide an overview and discussion of the reported traditional uses, botanical aspects, chemical constituents, and pharmacological potential of the Kalanchoe species. Published scientific materials were collected from the PubMed and SciFinder databases without restriction regarding the year of publication through April 2023. Ethnopharmacological knowledge suggests that these species have been used to treat infections, inflammation, injuries, and other disorders. Typically, all parts of the plant are used for medicinal purposes either as crude extract or juice. Botanical evaluation can clarify species differentiation and can enable correct identification and validation of the scientific data. Flavonoids are the most common classes of secondary metabolites identified from Kalanchoe species and can be correlated with some biological studies (antioxidant, anti-inflammatory, and antimicrobial potential). This review summarizes several topics related to the Kalanchoe genus, supporting future studies regarding other unexplored research areas. The need to conduct further studies to confirm the popular uses and biological activities of bioactive compounds is also highlighted. [ABSTRACT FROM AUTHOR]

Cowpea (Vigna unguiculata) is one of the main edible legume vegetables in China, and it can improve spleen and stomach function. A polysaccharide component (VUP80-3) has been isolated from V. unguiculata in this study. The average molecular weight of VUP80-3 is 6.43 × 104 Da, and the main monosaccharide group is glucose. The mass ratio of monosaccharide groups in the polysaccharide was glucose:galactose:arabinose:rhamnose:xylose:mannose:fucose = 152.36:24.50:16.53:8.13:1.26:0.97:0.82. NMR analysis showed that VUP80-3 has →4)-α-D-Galp (1→ and →4)-α-D-Glcp(1→ main chain and →3,4)-β-D-Glcp(1→, →4,6)-α-D-Glcp(1→ branch chains, and the terminal sugar is α-D-Glcp(1→. Biological activity test results showed that VUP80-3 at 1000 μg·mL−1 significantly increased the activity of ethanol injured GES-1 cells (p < 0.01) and significantly reduced reactive oxygen species (ROS) in ethanol injured GES-1 cells and inflammatory factors (IL-8, IL-1β and TNF-α,) in GES-1 cells. This compound also reduced the apoptosis rate (p < 0.05), thereby significantly reducing the oxidative damage caused by ethanol in GES-1 cells. Therefore, VUP80-3 is a potential drug to protect the gastric mucosa from damage. [ABSTRACT FROM AUTHOR]

Failure mode and effect analysis (FMEA) is one of the most used techniques in risk management due to its potential to solve multidisciplinary engineering problems. The role of experts is fundamental when developing the FMEA; they identify the failure modes by expressing their opinion based on their experience. A relevant aspect is a way in which the experts evaluate to obtain the indicator of the risk priority number (RPN), which is based on qualitative analysis and a table of criteria where they subjectively and intuitively determine the factor level (severity, occurrence, and detection) for each of the failures. With this, imprecision is present due to the interpretation that each one has regarding the failures. Therefore, this research proposes a fuzzy logic evaluation system with a solid mathematical basis that integrates these conditions of imprecision and uncertainty, thus offering a robust system capable of emulating the evaluation form of experts to support and improve decision making. One of the main contributions of this research is in the defuzzification stage, adjusting the centroid method and treating each set individually. With this, the RPN values approximate to the conventional technique were obtained. Simulations were carried out to test and determine the system's best structure. The system was validated in a textile company in southern Guanajuato. The results demonstrate that the system reliably represents how experts perform risk assessment. [ABSTRACT FROM AUTHOR]

Maize (Zea mays) is the most consumed food in the world. The leafhopper, Dalbulus maidis, (Hemiptera: Cicadellidae) is one of the most important maize pests, and due to its direct and indirect damage, it can cause losses of up to 100%. Sampling plans are essential components for integrated pest management programs. The first two components to be determined in these plans are the sampling unit and the sampling technique. The sampling unit consists of determining the plant organ to be evaluated in the sampling and the techniques are the devices used to sample populations of pests in crops. Thus, the objective of this work was to select the unit and technique to be used in the sampling of D. maidis in maize crops. For this purpose, pest populations in plants at stages V4, V6, V8, and V10 were monitored in 16 commercial crops in the Atlantic Forest and Cerrado biomes over two years. The sample units studied were the leaves at different positions in the canopy of the plants. The techniques studied were direct counting, shaking the apex of the plants in a plastic tray and using a beating cloth. In the process of selecting the unit and technique to be used in sampling populations of pests in crops, adequate criteria must be used. These criteria include precision, representativeness, sampling time and that the use of these components in the sampling plans should not cause damage to the plants. The most suitable sampling unit for evaluating these pest populations was the whorl leaves and the best technique was the direct counting. Therefore, the unit and technique selected in this work can be used in sampling plans for D. maidis in maize crops. [ABSTRACT FROM AUTHOR]

Bioluminescence is a common phenomenon in nature, especially in the deep ocean. The physiological role of bacterial bioluminescence involves protection against oxidative and UV stresses. Yet, it remains unclear if bioluminescence contributes to deep-sea bacterial adaptation to high hydrostatic pressure (HHP). In this study, we constructed a non-luminescent mutant of ΔluxA and its complementary strain c-ΔluxA of Photobacterium phosphoreum ANT-2200, a deep-sea piezophilic bioluminescent bacterium. The wild-type strain, mutant and complementary strain were compared from aspects of pressure tolerance, intracellular reactive oxygen species (ROS) level and expression of ROS-scavenging enzymes. The results showed that, despite similar growth profiles, HHP induced the accumulation of intracellular ROS and up-regulated the expression of ROS-scavenging enzymes such as dyp, katE and katG, specifically in the non-luminescent mutant. Collectively, our results suggested that bioluminescence functions as the primary antioxidant system in strain ANT-2200, in addition to the well-known ROS-scavenging enzymes. Bioluminescence contributes to bacterial adaptation to the deep-sea environment by coping with oxidative stress generated from HHP. These results further expanded our understanding of the physiological significance of bioluminescence as well as a novel strategy for microbial adaptation to a deep-sea environment. [ABSTRACT FROM AUTHOR]

Scorpions are incredible venomous animals found on almost every continent. According to fossil data, these animals have been able to adapt to the different environments from the Cambrian period until today with minimal anatomical changes. Scorpions are mostly nocturnal animals, and their ability to detect and tolerate light stimuli seems to be an essential tool for their subsistence, homing and mating. Centruroides suffuses is the most predominant specie of scorpions in Durango City, Mexico. Interestingly, and despite their life-threatening venom, these predatory arthropod animals have been adopted by locals as part of the landscape and daily life, by including them as part of their folklore and their economic resources, and learning how to take advantage of their abundance. In addition, the venom of scorpions possesses potential for therapeutic uses, while the scorpions themselves represent a nutritional food resource rich in protein, which has been poorly explored so far. Therefore, they are an excellent model for exploring the interplay between light sensibilities, survival and therapeutic–medicinal uses. Here, we review some of the potential benefits of scorpions and share the ways people in Durango City, Mexico, use UV light devices to detect and avoid or catch them for business and research purposes. [ABSTRACT FROM AUTHOR]

Naproxen is one of the most used non-steroidal anti-inflammatory drugs (NSAIDs). It is used to treat pain of various origins, inflammation and fever. Pharmaceutical preparations containing naproxen are available with prescription and over-the-counter (OTC). Naproxen in pharmaceutical preparations is used in the form of acid and sodium salt. From the point of view of pharmaceutical analysis, it is crucial to distinguish between these two forms of drugs. There are many costly and laborious methods to do this. Therefore, new, faster, cheaper and, at the same time, simple-to-perform identification methods are sought. In the conducted studies, thermal methods such as thermogravimetry (TGA) supported by calculated differential thermal analysis (c-DTA) were proposed to identify the type of naproxen in commercially available pharmaceutical preparations. In addition, the thermal methods used were compared with pharmacopoeial methods for the identification of compounds, such as high-performance liquid chromatography (HPLC), Fourier-transform infrared spectroscopy (FTIR), UV-Vis spectrophotometry, and a simple colorimetric analyses. In addition, using nabumetone, a close structural analog of naproxen, the specificity of the TGA and c-DTA methods was assessed. Studies have shown that the thermal analyses used are effective and selective in distinguishing the form of naproxen in pharmaceutical preparations. This indicates the potential possibility of using TGA supported by c-DTA as an alternative method. [ABSTRACT FROM AUTHOR]

Mucosal drug delivery permits direct and prompt drug absorption, which is capable of reducing undesirable decomposition that occurs before absorption. However, mucus clearance of those mucosal drug delivery systems strongly retards their actual application. Herein, we propose chromatophore nanoparticles embedded with FOF1-ATPase motors to promote mucus penetration. The FOF1-ATPase motor-embedded chromatophores were firstly extracted from Thermus thermophilus by using a gradient centrifugation method. Then, the model drug (curcumin) was loaded onto the chromatophores. The drug loading efficiency and entrapment efficiency were optimized by using different loading approaches. The activity, motility, stability and mucus permeation of the drug-loaded chromatophore nanoparticles were thoroughly investigated. Both the in vitro and in vivo studies revealed that the FOF1-ATPase motor-embedded chromatophore successfully enhanced mucus penetration glioma therapy. This study indicates that the FOF1-ATPase motor-embedded chromatophore is a promising alternative as a mucosal drug delivery system. [ABSTRACT FROM AUTHOR]

Fire spread behavior models are used to estimate fire behavior metrics, fire hazard, exposure, and risk across the landscape. One of the most widely used fire spread models is the minimum travel time (MTT), which requires a very time-consuming, interactive, trial-and-error calibration process to reproduce observed fire regimens. This study presents the MTTfireCAL package for R, a tool that enables fast calibration of the MTT fire spread models by testing and combining multiple settings and then ranking them based on the model's capacity to reproduce historical fire patterns, such as fire size distribution and fire frequency. Here, we explain the main methodological steps and validate the package by comparing it against the typical calibration procedures in two study areas. In addition, we estimate the minimum number of fire runs required to ensure a reliable calibration. Overall, the use of MTTfireCAL R package and the optimization of the number of ignitions used allowed for a faster calibration of the MTT modeling system than the typical trial-and-error calibration. The MTT modeling system calibrated using MTTfireCAL was also able to better reproduce the historical fire patterns. This tool has the potential to support the academic and operational community working with MTT. [ABSTRACT FROM AUTHOR]

Sporotrichosis, an invasive fungal infection caused by Sporothrix schenckii, has emerged in Southeast Asia, affecting cats and posing a potential zoonotic risk to humans. We evaluated 38 feline sporotrichosis cases in and around Bangkok, Thailand, from 2017 to 2021. The isolates were phenotypically and genotypically characterized. The cats infected with sporotrichosis were mainly young adults, males, and domestic short hairs with uncontrolled outdoor access, and they lived in Bangkok. All isolates showed low thermotolerance and converted to the yeast phase at 35 °C. Based on the internal transcribed spacer region of rDNA sequences, our strains belonged to S. schenckii sensu stricto and clustered with clinical clade D. Based on the concatenated tree of calmodulin and beta-tubulin genes, five groups of S. schenckii were generated, and the monophyletic clade, Group II, of Thai strains was recognized. In vitro antifungal susceptibility testing demonstrated that the MIC50 of our isolates to amphotericin B, itraconazole, and posaconazole were within the limit of the species-specific epidemiological cutoff values, suggesting that the organisms were the wild type. Addressing the outbreak of feline sporotrichosis in Thailand by providing guidelines for diagnosis and effective treatment may help control the spread of disease and reduce the risk of cat-transmitted sporotrichosis to humans. [ABSTRACT FROM AUTHOR]

Sporotrichosis is known as a subacute or chronic infection, which is caused by thermodimorphic fungi of the genus Sporothrix. It is a cosmopolitan infection, which is more prevalent in tropical and subtropical regions and can affect both humans and other mammals. The main etiological agents causing this disease are Sporothrix schenckii, Sporothrix brasiliensis, and Sporothrix globosa, which have been recognized as members of the Sporothrix pathogenic clade. Within this clade, S. brasiliensis is considered the most virulent species and represents an important pathogen due to its distribution and prevalence in different regions of South America, such as Brazil, Argentina, Chile, and Paraguay, and Central American countries, such as Panama. In Brazil, S. brasiliensis has been of great concern due to the number of zoonotic cases that have been reported over the years. In this paper, a detailed review of the current literature on this pathogen and its different aspects will be carried out, including its genome, pathogen-host interaction, resistance mechanisms to antifungal drugs, and the caused zoonosis. Furthermore, we provide the prediction of some putative virulence factors encoded by the genome of this fungal species. [ABSTRACT FROM AUTHOR]

Adhesives are used to join and seal electronic packaging. In these application, they are often used to bond aluminum components and, during the process of machining aluminum, oils and lubricants are used to facilitate the process. To remove these fluids, detergents such as surfactants are often used. However, if the surfactant itself is not removed completely and remains on the aluminum part, it can affect the bond properties of the joint, leading to abrupt joint failure. In this work, adhesive joint properties as a function of the amount of surfactant at the surface were studied. The joint substrates are Al6082-T6 aluminum, the adhesive a two-part silicone and the surfactant a cocosalkylaminethoxylate. For this, double cantilever beam (DCB) and single lap joints (SLJ) were used. An additional scanning electron microscopy (SEM) analysis was conducted to clarify the results. It was found that the adhesive bond strength continuously decreases with increasing amount of contaminant. Similarly, with increasing amounts of contaminant applied to the surface, the failure mode progressively changed from cohesive to adhesive, which was attributed to the adhesive at the interphase absorbing the contaminant during curing, moving the failure to this region, until the absorption limit of the adhesive is surpassed and the surfactant remains at the interface, leading to interfacial failure. A numerical model was created to simulate the behavior of the experimental joints and obtain the properties of the interphase by applying the inverse method, and a numerical study was done on the sensitivity of the model to variations in cohesive peel properties for the DCB and shear properties for the SLJ. [ABSTRACT FROM AUTHOR]

Failure modes, effects, and criticality analysis (FMECA) is a qualitative risk analysis method widely used in various industrial and service applications. Despite its popularity, the method suffers from several shortcomings analyzed in the literature over the years. The classical approach to obtain the failure modes' risk level does not consider any relative importance between the risk factors and may not necessarily represent the real risk perception of the FMECA team members, usually expressed by natural language. This paper introduces the application of Type-I fuzzy inference systems (FIS) as an alternative to improve the failure modes' risk level computation in the classic FMECA analysis and its use in cyber-power grids. Our fuzzy-based FMECA considers first a set of fuzzy variables defined by FMECA experts to embody the uncertainty associated with the human language. Second, the "seven plus or minus two" criterion is used to set the number of fuzzy sets to each variable, forming a rule base consisting of 125 fuzzy rules to represent the risk perception of the experts. In the electrical power systems framework, the new fuzzy-based FMECA is utilized for reliability analysis of cyber-power grid systems, assessing its benefits relative to a classic FMECA. The paper provides the following three key contributions: (1) representing the uncertainty associated with the FMECA experts using fuzzy sets, (2) representing the FMECA experts' reasoning and risk perception through fuzzy-rule-based reasoning, and (3) applying the proposed fuzzy approach, which is a promissory method to accurately define the prioritization of failure modes in the context of reliability analysis of cyber-power grid systems. [ABSTRACT FROM AUTHOR]

This systematic review aimed to assess the available evidence on the effects of neuromuscular training on physical performance in older adults. A literature search was conducted across four databases (Psychology and Behavioral (EBSCO), Scopus, Web of Science and PubMed). The PRISMA guidelines were followed. The PEDro scale and Cochrane risk of bias tool were used to assess the quality of and risk of bias in the studies, respectively. The protocol was registered in PROSPERO (code: CRD42022319239). The outcomes were muscle strength, cardiorespiratory fitness, postural balance and gait speed. From 610 records initially found, 10 were finally included in the systematic review, involving 354 older people with a mean age of 67.3 years. Nine of them reported significant changes in at least one variable related to physical performance in the intervention compared to the control groups. The neuromuscular training caused significant improvements in postural balance, flexibility, cardiorespiratory fitness, strength power of the upper and lower limbs and autonomy. The available evidence indicates that neuromuscular training has a positive effect on some variables of physical performance, especially in postural balance; however, the methodological quality and certainty of the evidence in the available literature are limited. Therefore, a greater number of high-quality studies are required to draw definitive conclusions. [ABSTRACT FROM AUTHOR]

Understanding the spatial variability of factors that influence crop yield is essential to apply site-specific management. The present study aimed to evaluate apparent soil electrical conductivity (ECa) in two fields (A = rainfeed; B = central-pivot irrigation), based on delimited management zones (MZs). In each MZ, the soil density (Sd) was characterized at two soil depths, and whether the delimitation of MZs, based on the spatial variability of ECa, was able to identify regions of the field with different Sd was assessed. In general, MZs with the highest mean value of ECa also presented the highest mean values of Sd. The highest Sd values were observed in the 0.1–0.2 m layer, regardless of the studied area. Regardless of soil texture, the proposed ECa was able to detect in-field differences in Sd. The delimitation of MZs, based on the spatial variability of ECa mapping, was able to differentiate the mean values of Sd between MZ 1 (1.53 g cm−3) and MZ 2 (1.67 g cm−3) in field A, in the 0.1–0.2 m layer. A statistical difference was observed for the mean values of Sd, in MZ 1, at layer 0.1–0.2 m, when comparing the two fields: A (1.53 g cm−3) and B (1.64 g cm−3). We suggest that further studies should be carried out to confirm the efficiency of ECa in detecting the soil bulk density at different soil depths. [ABSTRACT FROM AUTHOR]