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The aim of this study was the genotyping of Yersinia pseudotuberculosis strains isolated in Russia using multilocus sequence typing (MLST), a comparative analysis of MLST results with INDEL typing results. Materials and methods. 348 strains of various serotypes isolated in different regions of the world, including 59 Russian strains, were analyzed. 302 strains from the NCBI database were used. The nucleotide sequences of the strains were determined using the MiSeq technology platform. The author MLSTtyper program was used for MLST analysis. Results and discussion. It has been found out that strains of at least, 2, 9, 19, 26, 32, 42, 43 ST-types circulate in Russia. The majority of the studied Russian strains belong to the ST2 type (58 %). Phylogenetic analysis conducted using MLST data showed that a number of clusters contains strains that have been circulating in Russia for a long time, and strains of these genotypes are not found abroad. Some of the Russian strains are included in clusters containing strains isolated in various regions of the world and can be attributed to strains of either “Asian” or “European” types. The data obtained using the MLSTtyper program coincided with the data of foreign authors when analyzing the same strains using the MLST method. Comparison of the results of genetic differentiation of Y. pseudotuberculosis strains using MLST and INDEL typing indicates that MLST has a greater discriminatory ability; however, in some cases INDEL genotyping allows differentiating those strains that had the same ST genotype.

RELEVANCE of the study lies in the analysis of the influence of specifying the wind speed interval on the calculation of electricity generation by certain wind turbines used in the Crimea. PURPOSE. Analysis of techniques that can be used to estimate electric power generation by wind turbines in various cases, as well as an assessment of the impact on the accuracy of the forecast of the speed indication interval when using "semi-aggregated" data. METHODS. Analytical and computational methods were used in the study, in particular, the variable substitution method, Rayleigh distribution, and the Milewski method. RESULTS. In the article the methods of calculating the electric power generation for three cases are considered. The first case uses primary observational data, so it is applicable only when a weather station is directly present in the area. The second case describes the course of calculations when the wind characteristics sensors are partially shaded and when the terrain is more complex. Here it is necessary to classify the degree of openness of the wind speed sensor. If the site is located far from weather stations or weather posts, the third method is used. In this case, the choice of interpolation nodes of the indicators of potential power generation can be quite complex. We also evaluated the effect of wind speed on electricity generation by a wind turbine. The number of aggregation intervals and the aggregation interval itself were changed, and the results were found for two wind turbines USW56-100 and T600-48, common in the Crimea. CONCLUSIONS. Wind energy is one of the promising areas, but often because of the differences between the forecast data and the actual, there are difficulties in integrating into the overall energy system. Therefore, it is important to develop methods for estimating the generation and accuracy in their use.

The ontological status of the game, according to the authors, today needs a particularly thorough analysis, and traditional approaches to game research require a deeper methodological justification. The growing importance of game culture in modern social dynamics sharply raises questions about the essence of games, to which the previous paradigm, based on an activity approach, did not give clear answers. The hypothesis of the study is that the game is one of the oldest ontological practices and serves to identify the ethical, semantic, existential boundaries of certain everyday situations in which a person develops acceptable or possible behavioral strategies for himself. In this status, game is comparable to ritual and performs similar functions in social dynamics. The study used phenomenological and systemic methods with elements of the structural-functional approach and the method of logical reconstruction, as an auxiliary toolkit of sociological research was used, in particular, the focus group method. As a result of the study, the authors came to the conclusion that modern computer, virtual games act as tools for self-knowledge for users, and also serve to mark socially acceptable and unacceptable behavior patterns. Social reality plays a double role here. On the one hand, it forms a dichotomous relationship with virtual reality, which allows the player to form their own ethical principles related to behavior in various environments. On the other hand, social reality is a source for game plots, implicitly offering the player to treat it contemplatively and detachedly. Thus, the study revealed a new socio-cultural significance of the game in social and personal development. The proposed methodology for the interpretation of games is a universal method, with the help of which previously difficult to resolve issues, in particular, the phenomenon of ludomania, can be clearly verified.

The aim of this study was to develop a new method of intraspecific genetic differentiation of Yersinia pseudotuberculosis, based on the detection of INDEL-markers using PCR. Materials and methods. Analyzed were 308 strains from the NCBI database and 15 strains sequenced within the frames of this study. The nucleotide sequences of the strains were determined using the MiSeq technology platform. The genomes of the strains sequenced in the work, as well as genomes from the NCBI database, were assessed using in silico PCR with 7 pairs of primers designed in the study. As a result of a comparison of genome-wide sequences of 22 Y. pseudotuberculosis strains from the NCBI database, using the author’s software (GenExpert), 7 INDEL-markers were selected that make it possible to effectively distinguish between strains of the causative agent of pseudotuberculosis. Based on these markers, 7 pairs of primers were designed and synthesized for the analysis of different strains using PCR. Analysis of 323 strains in PCR in silico and 70 strains in PCR in vitro allowed for dividing them into 30 genetic groups. Comparison of the results of PCR in silico and in vitro confirmed the possibility of using the proposed primers for intraspecific differentiation of Y. pseudotuberculosis. Based on the data obtained, a dendrogram reflecting the phylogenetic relations of different strains of Y. pseudotuberculosis was constructed. When analyzing the distribution of Y. pseudotuberculosis strains by various clusters and genetic groups, a number of patterns were revealed. Conducted in silico and in vitro PCR show that the proposed method of INDEL-typing can be used for intraspecific genetic differentiation of the causative agent of pseudotuberculosis.

Importance: Antiphospholipid syndrome in neonates and children is a rare, but in some cases life-threatening condition with arterial and/or venous thrombosis and/or non-thrombotic neurological, skin, ophthalmological and other manifestations. Observations: This review highlights the available information about the features of pediatric APS, including the rare catastrophic form, the differences between pediatric and adult APS, and the role of genetic thrombophilia in APS manifestation. Conclusions and relevance: The clinical manifestations and treatment options for APS in children may differ from those in adults, and prescribing therapy can be challenging due to the unique clinical and morphological characteristics of the pediatric patient. Pediatric APS may be a predictor of the development of certain autoimmune diseases and classic manifestations of APS in adulthood, therefore, a revision of the existing criteria for the diagnosis and treatment of APS in children is necessary. [ABSTRACT FROM AUTHOR]

Neologisms that appeared under the influence of the pandemic of a new coronavirus infection Covid-19 are considered, based on the material of English, French and German languages. The basis of the study is formed by neologisms collected by continuous sampling from available lexicographic sources and foreign language Internet resources. The relevance of this work is confirmed by the rapid growth of neologisms in all the languages under consideration. The novelty of the study lies in a comparative approach to the analysis of nomination processes and in determining the dominant areas of semantic attraction in these linguistic cultures. The elements that have shown the greatest productivity and stable functioning are singled out. The author’s classification of covid neologisms is proposed. The question is raised about the susceptibility of these languages to new realities, their productivity in the process of responding to changes in various spheres of society. It is concluded that the number of English neologisms prevails over the corresponding units in French and German. It is confirmed that the structure of the lexico-semantic groups identified during the analysis is heterogeneous and in some cases has a lacunar character. It is suggested that the reason for this may be both the specificity of the word formation of languages, and the peculiarities of the mentality inherent in a particular linguocultural community.

See the retraction notice E3S Web of Conferences 420, 00001 (2023), https://doi.org/10.1051/e3sconf/202342000001