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Copyright of Journal of Cryptologic Research (2097-4116) is the property of Editorial Board of Journal of Cryptologic Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To explore the effects of health education based on the transtheoretical model (TTM) on intestinal preparation quality and psychological stress of patients undergoing endoscopic treatment. Methods A total of 134 patients treated with digestive endoscopy in the Second People's Hospital Zhengzhou from February 2021 to October 2022 were selected and divided into the observation group(n=67) and the control group(n=67) according to the random number table method. The control group received routine nursing intervention, and the observation group received health education intervention based on TTM. The quality of intestinal readiness [Boston Bowel Preparation Scale(BBPS), Ottawa Bowel Preparation Scale (OBPS)], psychological stress [Connor-Davidson resilience scale(CD-RISC)],complications(nausea. choking, abdominal pain, bloating), quality of life [Activity of Daily Living Scale(ADL)] and nursing satisfaction of the two groups were statistically compared. Results After intervention, BBPS, CD-RISC and ADL scores in the observation group were higher than those in the control group, and the OBPS scores were lower than those in the control group, and the differences were statistically significant (P<0.05). After the intervention, the incidence of complications in the observation group was 1.49%, lower than that in the control group(11.94%), and the difference was statistically significant(P<0.05). After intervention, the satisfaction of the observation group was 100.00%, higher than that of the control group (88.06%), the difference was statistically significant(P<0.05). Conclusion Health education based on TTM can regulate the psychological stress of patients undergoing endoscopic treatment, improve the quality of intestinal preparation, reduce complications. improve the quality of life, and enhance nursing satisfaction. [ABSTRACT FROM AUTHOR]

Copyright of Modern Food Science & Technology is the property of Editorial Office of Modern Food Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To study the methods of primary culture and purity identification of bone marrow mesenchymal stem cells. Methods: In a sterile environment, the bone marrow was collected from the femur and tibia of SD rats, digested with enzymes first, and subcultured the extracted BMSCs by using the whole bone marrow cell suspension adherence method, and the third generation cells with good growth were selected for identification; BMSCs were induced to differentiate into adipogenic and osteogenic, and the differentiation effect of BMSCs was identified by oil red O (ORO) and alizarin red (ARS) staining; CD34, CD44 and CD90 were detected by flow cytometry (FCM). The expression of three types of BMSCs surface markers was analyzed. Results: BMSCs are long spindle-shaped adherent cells, and the growth state is fibroblast-like swirl. During the passage of the third generation BMSCs, they developed to the growth latent period on the 1st-3d, showing a slower growth rate, and developed on the 3rd-5d. In the logarithmic growth phase, it grows at a high speed. After the 7th day, the growth speed reaches the maximum, and the speed stops increasing and enters the plateau phase. After the induction of osteogenesis and adipogenicity of BMSCs, the identification of the induced differentiation found that the cells showed obvious morphological changes, Staining fat by ORO, the cells showed orange red; after the end of osteogenic induction culture, staining calcium salt by ARS showed red, and mineralized nodules appeared, indicating that BMSCs had good osteogenic and adipogenic differentiation capabilities; FCM It was found that the expression of CD34 was negative (1.09 %), and both CD90 (96.8 %) and CD44 (92.4 %) were positive, which was consistent with the phenotype of BMSCs. Conclusion: BMSCs were effectively isolated and cultured by the whole bone marrow adherent culture technique. [ABSTRACT FROM AUTHOR]

Copyright of Modern Food Science & Technology is the property of Editorial Office of Modern Food Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)