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【Objective】The aim of this paper is to analyze the genetic diversity of faba bean germplasm resources by transcriptome sequencing and SSR molecular marker technology， and to explore the molecular markers associated with bruchid resistance traits in faba bean. So as to provide a reference basis for the conservation and utilization of bruchid resistance germplasm resources and the selection and breeding of new varieties. 【Method】Transcriptome sequencing of two faba bean varieties Y134 and Y078 was performed by PacBio triple full-length sequencing technology combined with RNA-seq.. Based on this， all Unigene of the transcriptome were searched for Unigene containing SSR core motifs using the software MISA， and primers were designed in batch using Primer 3.0. One hundred pairs of SSR primers were randomly selected for genetic diversity analysis of 80 faba bean germplasm resources. 【Result】Fifty of the 100 SSR primer pairs amplified products， with an effective amplification rate of 50%; 32 of the primer pairs showed polymorphism. They accounted for 64% of the effective products and 32% of the total primers. Among the 32 polymorphic primer pairs， each pair amplified 2 to 21 alleles with an average of 6.875; The polymorphic information content （PIC） values ranged from 0.25 to 0.82 with an average of 0.58; The Shannon’s information index varied from 0.34 to 2.31 with an average of 1.21. Based on UPGMA cluster analysis， the 80 faba bean materials were classified into two major groups at a similarity coefficient of 0.70. The first category contained 65 germplasm， including 62 highly resistant （HR） varieties， 2 highly sensitive （HS） varieties and 1 sensitive （S） variety; The second category contained 15 germplasm， 7 highly susceptible （HS） varieties， 2 sensitive （S） varieties， 5 highly resistant （HR） varieties and 1 moderately resistant （MR） variety. 【Conclusion】The SSR markers developed were used to analyze the genetic diversity of 80 faba bean germplasm resources and to lay the theoretical foundation for research on the identification of faba bean germplasm resources resistant to bean weevil and molecular marker-assisted selection for breeding. [ABSTRACT FROM AUTHOR]