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Summary: In heterogeneous access network, Multiple‐Input Multiple‐Output (MIMO) radio‐over‐fiber (RoF) system is an efficient approach for multiple signal transmission with low cost and complexity. The performance of RoF fronthaul system in MIMO system will be varied with different nonlinear effects. By adjusting various transmission parameters, such as the input signal power or the laser bias current, the nonlinear impacts produced by the RoF system can be reduced. In this paper, a novel algorithm Improved Aquila Optimization (IAO) is proposed to optimize transmission circumstances of MIMO RoF system. It determines the appropriate bias current for both lasers and Radio Frequency (RF) signal power in a short period. The input signals are wavelength multiplexed with Intensity Modulation and Direct Detection (IM/DD) applied. The carrier as well as transmission frequency is governed by the MIMO‐Long‐Term Evolution (LTE) standard. The proposed system is implemented in MATLAB, and the performance is evaluated. The experimental results show that fast convergence and trade‐off between noise and nonlinearity are obtained with varying bandwidth. In the experimental scenario, the maximum Error Vector Magnitude (EVM) of 1.88, 3.14, and signal‐to‐noise ratio (SNR) of 3.204, and 2.698 was attained for both quadrature phase shift keying (QPSK) and quadrature amplitude modulation (QAM) modulation. [Correction added on 24 April 2023, after first online publication: the SNR values were corrected in the preceding sentence.] For 100 iterations, the processing time was reduced to 0.137 s. When compared with the conventional state‐of‐the‐art approaches, the accuracy and computational complexity of the proposed approach are improved. [ABSTRACT FROM AUTHOR]