1. Exposure to O3 and NO2 on the interfacial chemistry of the pulmonary surfactant and the mechanism of lung oxidative damage.
- Author
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Li, Jie, Song, Haoran, Luo, Tao, Cao, Yan, Zhang, Linfeng, Zhao, Qun, Li, Zhanchao, Hu, Xuewei, Gu, Junjie, and Tian, Senlin
- Subjects
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PULMONARY surfactant , *REACTIVE oxygen species , *HYDROXYL group , *SURFACE tension , *RESPIRATORY organs - Abstract
Exposure to ozone (O 3) and nitrogen dioxide (NO 2) are related to pulmonary dysfunctions and various lung diseases, but the underlying biochemical mechanisms remain uncertain. Herein, the effect of inhalable oxidizing gas pollutants on the pulmonary surfactant (PS, extracted from porcine lungs), a mixture of active lipids and proteins that plays an important role in maintaining normal respiratory mechanics, is investigated in terms of the interfacial chemistry using in-vitro experiments; and the oxidative stress induced by oxidizing gases in the simulated lung fluid (SLF) supplemented with the PS is explored. The results showed that O 3 and NO 2 individually increased the surface tension of the PS and reduced its foaming ability; this was accompanied by the surface pressure-area isotherms of the PS monolayers shifting toward lower molecular areas, with O 3 exhibiting more severe effects than NO 2. Moreover, both O 3 and NO 2 produced reactive oxygen species (ROS) resulting in lipid peroxidation and protein damage to the PS. The formation of superoxide radicals (O 2 •–) was correlated with the decomposition of O 3 and the reactions of O 3 and NO 2 with antioxidants in the SLF. These radicals, in the presence of antioxidants, led to the formation of hydrogen peroxide and hydroxyl radicals (•OH). Additionally, the direct oxidation of unsaturated lipids by O 3 and NO 2 further caused an increase in the ROS content. This change in the ROS chemistry and increased •OH production tentatively explain how inhalable oxidizing gases lead to oxidative stress and adverse health effects. In summary, our results indicated that inhaled O 3 and NO 2 exposure can significantly alter the interfacial properties of the PS, oxidize its active ingredients, and induce ROS formation in the SLF. The results of this study provide a basis for the elucidation of the potential hazards of inhaled oxidizing gas pollutants in the human respiratory system. [Display omitted] • Oxidizing gases impacted the surface tension, phase behavior, and foaming ability of the PS. • Lipid peroxidation and protein damage of the PS were induced by oxidizing gas-phase air pollutants. • O 3 exhibited stronger adverse effects to the PS in comparison to NO 2. • Both ROS and oxidizing gas air pollutants contributed to oxidative damage of the PS. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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