1. Metarhizium anisopliae E6 secretome reveals molecular players in host specificity and toxicity linked to cattle tick infection.
- Author
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Saciloto-de-Oliveira, Laura Rascovetzki, Broetto, Leonardo, Alves, Camila Innocente, da Rosa, Rafael Lopes, Calegari Alves, Yohana Porto, da Silva, Rodrigo Campos, Berger, Markus, Macedo, Alexandre José, Dalberto, Pedro Ferrari, Bizarro, Cristiano Valim, Guimarães, Jorge Almeida, Yates, John R., Santi, Lucélia, and Beys-da-Silva, Walter Orlando
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CATTLE tick , *METARHIZIUM anisopliae , *ENTOMOPATHOGENIC fungi , *BEAUVERIA bassiana , *PROTEOMICS , *PROTEIN expression , *GREATER wax moth , *BIOLOGICAL pest control agents , *ACARICIDES - Abstract
Although Metarhizium anisopliae is one of the most studied fungal biocontrol agents, its infection mechanism is far from being completely understood. Using multidimensional protein identification technology (MudPIT), we evaluated the differential secretome of M. anisopliae E6 induced by the host Rhipicephalus microplus cuticle. The proteomic result showed changes in the expression of 194 proteins after exposure to host cuticle, such as proteins involved in adhesion, penetration, stress and fungal defense. Further, we performed a comparative genomic distribution of differentially expressed proteins of the M. anisopliae secretome against another arthropod pathogen, using the Beauveria bassiana ARSEF2860 protein repertory. Among 47 analyzed protein families, thirty were overexpressed in the M. anisopliae E6 predicted genome compared to B. bassiana. An in vivo toxicity assay using a Galleria mellonella model confirmed that the M. anisopliae E6 secretome was more toxic in cattle tick infections compared to other secretomes, including B. bassiana with cattle ticks and M. anisopliae E6 with the insect Dysdereus peruvianus , which our proteomic results had also suggested. These results help explain molecular aspects associated with host infection specificity due to genetic differences and gene expression control at the protein level in arthropod-pathogenic fungi. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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