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Objective: To study the relationship between the angiomotin (Amot) and Yes-associated protein 1 (YAP1) expression and clinicopathological parameters and prognosis in type I endometrial cancer (EC) tissues. Methods: 75 type I EC patients who were diagnosed and treated in Affiliated Hospital of Xuzhou Medical University from January 2014 to June 2017 were selected as the study object (EC group), 30 patients with pathologically confirmed atypical hyperplasia endometrial tissue specimens diagnosed and treated during the same period were selected as the atypical hyperplasia endometrial group, and 30 patients with pathologically confirmed normal endometrial tissue specimens during the same period were selected as the normal endometrial group. The Amot and YAP1 protein expressions in each group were detected by immunohistochemistry. Spearman rank correlation was used to analyze the correlation between Amot and YAP1 protein expression in type I EC cancer tissues. The differences of Amot and YAP1 protein expression in cancer tissues of type I EC patients with different clinicopathological characteristics were compared. Kaplan-Meier analysis (Log-rank test) was used to analyze the relationship between the expression of Amot and YAP1 protein and the survival prognosis of type I EC patients. Univariate and multivariate COX regression analysis was used to analyze the factors affecting the survival prognosis of type I EC patients. Results: The positive rates of Amot and YAP1 protein expression in type I EC tissues were 80.00%(60/75) and 85.33%(64/75), respectively, which were significantly higher than those in atypical hyperplasia endometrial tissue 43.33% (13/30) and 46.67% (14/30), and the difference was statistically significant (all P＜0.05). The positive rates of Amot and YAP1 protein in atypical hyperplasia endometrial tissue were significantly higher than those in normal endometrial tissue with 16.67% (5/30) and 20.00% (6/30), and the difference was statistically significant (all P＜0.05). There was a significant positive correlation between Amot and YAP1 protein expression in type I EC cancer tissues(P＜0.05). The positive rates of Amot and YAP1 protein expression in EC type I patients with different International Federation of Gynecology and Obstetrics (FIGO) stages, histological grades and lymph node metastasis were statistically significant (P＜0.05). The cumulative survival time of Amot-positive expression typeⅠEC patients was significantly lower than that of Amot-negative expression typeⅠEC patients (P＜0.05). The cumulative survival time of patients with YAP1-positive expression of typeⅠEC was significantly lower than that of patients with YAP1-negative expression of typeⅠEC (P＜0.05). Amot-positive expression, YAP1 positive expression, FIGO stage Ⅲ and lymph node metastasis were independent risk factors for poor prognosis in patients with EC type I (P＜0.05). Conclusion: The Amot and YAP1 protein positive expression increased in type I EC cancer tissues, which are related to FIGO stage, histological grade and lymph node metastasis. The positive expression of Amot protein and YAP1 protein is one of the risk factors affecting the poor prognosis of patients with type I EC. [ABSTRACT FROM AUTHOR]

Background and purpose: Perineural invasion is a prognostic factor in colorectal cancer. It is unclear whether perineural invasion is a prognostic factor in in node-negative (ypⅠ-Ⅱ) rectal cancer patients who received neoadjuvant radiotherapy because there may be varying degrees of tumor regression. This study aimed to investigate prognostic value of perineural invasion in node-negative rectal cancer patients who received neoadjuvant radiotherapy. Methods: A total of 5 222 patients who received neoadjuvant radiotherapy and were diagnosed with stage ypⅠ-Ⅱ rectal cancer from the Surveillance, Epidemiology, and End Results (SEER) database between 2000 and 2018 were identified and clinicopathological features and cancer-specific survival outcomes were retrospectively analyzed. Chi-square analysis was used to compare clinicopathological features between two groups. Kaplan- Meier survival analysis and the log-rank test were used to estimate the 5-year cancer-specific survival. Multivariable Cox regression analyses were used to identify the independent prognostic factor. Results: Compared with patients without perineural invasion, patients with perineural invasion were more likely to be black, have elevated carcinoembryonic antigen (CEA), stage Ⅱ disease, tumor＜5 cm and poorer differentiated tumors (P＜0.05). Patients without perineural invasion had superior cancer-specific survival than those with perineural invasion (5-years survival rate: 85.3% vs 68.9%). The survival difference was more evident in patients with stage ypⅡ cancer. Multivariable Cox regression analyses demonstrated that perineural invasion was an independent prognostic factor in node-negative rectal cancer patients who received neoadjuvant radiotherapy. Conclusion: Perineural invasion is associated with oncological outcomes of node-negative rectal cancer patients who received neoadjuvant radiotherapy, thus it could serve as a prognostic factor in these patients. [ABSTRACT FROM AUTHOR]